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The map label for this gene is topB [H]

Identifier: 238027970

GI number: 238027970

Start: 2720058

End: 2722226

Strand: Reverse

Name: topB [H]

Synonym: bglu_1g24160

Alternate gene names: 238027970

Gene position: 2722226-2720058 (Counterclockwise)

Preceding gene: 238027971

Following gene: 238027969

Centisome position: 69.68

GC content: 68.65

Gene sequence:

>2169_bases
GTGCGTCTTTGGATCTTCGAGAAACCGTCCATGGCCAAGGCCGTGATCCCCCATCTGCCGAAGCCGCACACGCGGCACGA
GGGCTACTACGAGACCGGCGACGGCTACGTGTCCTGGCTGGCCGGCCACGTGATGGAGCAGGTGCAGCCGGGCGACTACG
ACGAGCGCTGGGGCAAGTTCCCCTGGGCGTTCGAGGACCTGCCGATCATCCCGAAGGACTGGAAGCTCAAGGTCTCGGAG
GACAAGAAGGGCCAGGTCAAGATCATCGAGACGCTGCTGAAAAAGTGCACGTCGATCGTGAACGGCGGGGACGCCGACCG
CGAGGGCCAGCTCCTGGTCGACGAGGTGCTGCTGCACTTCGGCAACACCAAGCCCGTGGCGCGGATCCACCTGAGCGCGA
TGGACCCGACCAGCGTCAAGAAGGCGATCGCCTCGCTCGAGGACAACGCCACCTTCCGTCCGCTCTACGAGGCGGGCCTG
GGCCGGCAGCGCGCCGACTGGCTGGTGGGCATGAACATGTCCCGGGCCTACACCATCCTGGCGAAGAAGCAGAACTACCC
CGGCGTGCTGTCGGTCGGGCGCGTGCAATCGCCCACGCTCGCCCTGGTGGTCAAGCGCGACCGCGAGATCGACGGGTTCG
TCCCGAAGGACTTCTGGACCGTCGAAGCGCAGTTCGCCGACCCGGGCCAGTCGGCGTTGCCGTTCTGGTCGCGCTGGCTC
CCGCCGGGCCTGTCGCTCGAATCCGCGGAGAAGCAGGCGGACGTCGACGCGAAGGGCGAGGCCGACGAGGACGAGGAGGG
CGACGAGATCGAGGGCGAGGGCGGCACCGTCACCGGTCAGCGCCCGGCCTGGCTGGACGAGCAGAACCGCATCATCAACA
AGGCGGTGGCGGACCAGATCGCGGCGAATGTGCAGGCCGCCGGCCAGGGCACCGTCACGCGCTACGTGAACAAGCCGGCG
GAGGAGCAGCCCCCGCTGCCGTTCGAGCTGACCGGTCTGCAGACCGCGCTGAACGGGAAGTACGGCTACTCGGCCAAGGA
GGTGCTCGACACCTGCCAGGAGCTGTATCTCCAGGGGCACACCACCTACCCGCGCTCGGACTGCCCGTACCTGCCCGTAT
CGCAGCACGCGGAAGCCCCGGACGTGCTGGACGCGATCGCACGGGGCGCCCCGCACCTGGCCTCCCTGGCGGCTAACGCT
AACGTGACGATCCGTTCGCGGGTGTGGAACGACAGCAAGGTGACCGAGCACCACGCGCTGATCCCGACGCGCCAGGCCCC
GGACTGGTCGACCATGACCGAGATCAAGCGCCGCACGTACGAGATGATCGCGCAGCGCTACCTGGCCCAGCTCTACCCGA
ACTGCCAGGTGGACAAGGCCAAGGTCGAGGTGTCGATCGCGGGCGAGCGTTTCGCGGCAAGCGGTCGCGTGGTGAAGGAC
CCGGGCTGGCGCGTGGTGTTCCAGGGCGAGGCCCAGGCCACCGACGCGAAGAAGGCGAAGGACGCCACCCCGACCCTGCC
GGTGCTGACCGAGCAGGGCCCGGTGAACCGCCTGGCGGTGAGGGTGGACGCGAAGCGCACCACGCCCCCGCCGCGCTACA
CCGAGGGCTCGCTGCTGACGGCGATGAAGCACGTCCACCGGACCGTCACGGACCCGGTGGAGCGCAAGAAGCTCAAGGCC
CTGGACGGCATCGGCCGGTCGGCGACCCGCGCCGGGATCATCGAAATGATCATCAAGCGCGGCTTCGTGGAGGTGAAGGG
CAAGCAGCTCGTGGCCACCGTGGTCGGCAAGATCCTGGTGGACGCGTTGCCGGCCAAGCTCACGGACCCGGGCATGACCG
CCCGGTGGGAGGCCCTGCTCGACGCGATCGCGAAGAAGCAGATCCCGCTGCACGCGTTCATGGCGAAGCAGGAGGAGGCC
ATCGGCGAGCTGGTGGCCGCGGCGCGCGTCTCGACCCTGCCGACCATCCCGCCCGACGCGATGCCGGCGCGCAAGCCGTA
CACCGGATCGTCCACCCCGCGGCCGGCCCCGGACGGCGCGAAGAAGTGCCCGAAGTGCAAGAAGGGCTACCTGGTCCCCC
GGACGGTGAAGAACGGTCCGAAGGCGGGGCAGACCTTCCACGGGTGCAACAACTACCCGGAATGCAAGCACAGCGTCTGG
CCGAAGTAA

Upstream 100 bases:

>100_bases
GCCCCCTCCCGCAGCACCCTGACCCCCCTGGTTTGACAAGCATCTGGGACCTGCTAGGTACGAGTACCCGTGCCATGGCA
CAGATTGAAGGAGGCCCGCC

Downstream 100 bases:

>100_bases
GGCCGGGCTCCCGACGTTCACGAAGCCTGCCTGGCGACCACCGGGCGGGCTTTTTTCATGGCCATTCACCCGTTCCCATC
CAGGAGGTACCACCATGCAG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MRLWIFEKPSMAKAVIPHLPKPHTRHEGYYETGDGYVSWLAGHVMEQVQPGDYDERWGKFPWAFEDLPIIPKDWKLKVSE
DKKGQVKIIETLLKKCTSIVNGGDADREGQLLVDEVLLHFGNTKPVARIHLSAMDPTSVKKAIASLEDNATFRPLYEAGL
GRQRADWLVGMNMSRAYTILAKKQNYPGVLSVGRVQSPTLALVVKRDREIDGFVPKDFWTVEAQFADPGQSALPFWSRWL
PPGLSLESAEKQADVDAKGEADEDEEGDEIEGEGGTVTGQRPAWLDEQNRIINKAVADQIAANVQAAGQGTVTRYVNKPA
EEQPPLPFELTGLQTALNGKYGYSAKEVLDTCQELYLQGHTTYPRSDCPYLPVSQHAEAPDVLDAIARGAPHLASLAANA
NVTIRSRVWNDSKVTEHHALIPTRQAPDWSTMTEIKRRTYEMIAQRYLAQLYPNCQVDKAKVEVSIAGERFAASGRVVKD
PGWRVVFQGEAQATDAKKAKDATPTLPVLTEQGPVNRLAVRVDAKRTTPPPRYTEGSLLTAMKHVHRTVTDPVERKKLKA
LDGIGRSATRAGIIEMIIKRGFVEVKGKQLVATVVGKILVDALPAKLTDPGMTARWEALLDAIAKKQIPLHAFMAKQEEA
IGELVAAARVSTLPTIPPDAMPARKPYTGSSTPRPAPDGAKKCPKCKKGYLVPRTVKNGPKAGQTFHGCNNYPECKHSVW
PK

Sequences:

>Translated_722_residues
MRLWIFEKPSMAKAVIPHLPKPHTRHEGYYETGDGYVSWLAGHVMEQVQPGDYDERWGKFPWAFEDLPIIPKDWKLKVSE
DKKGQVKIIETLLKKCTSIVNGGDADREGQLLVDEVLLHFGNTKPVARIHLSAMDPTSVKKAIASLEDNATFRPLYEAGL
GRQRADWLVGMNMSRAYTILAKKQNYPGVLSVGRVQSPTLALVVKRDREIDGFVPKDFWTVEAQFADPGQSALPFWSRWL
PPGLSLESAEKQADVDAKGEADEDEEGDEIEGEGGTVTGQRPAWLDEQNRIINKAVADQIAANVQAAGQGTVTRYVNKPA
EEQPPLPFELTGLQTALNGKYGYSAKEVLDTCQELYLQGHTTYPRSDCPYLPVSQHAEAPDVLDAIARGAPHLASLAANA
NVTIRSRVWNDSKVTEHHALIPTRQAPDWSTMTEIKRRTYEMIAQRYLAQLYPNCQVDKAKVEVSIAGERFAASGRVVKD
PGWRVVFQGEAQATDAKKAKDATPTLPVLTEQGPVNRLAVRVDAKRTTPPPRYTEGSLLTAMKHVHRTVTDPVERKKLKA
LDGIGRSATRAGIIEMIIKRGFVEVKGKQLVATVVGKILVDALPAKLTDPGMTARWEALLDAIAKKQIPLHAFMAKQEEA
IGELVAAARVSTLPTIPPDAMPARKPYTGSSTPRPAPDGAKKCPKCKKGYLVPRTVKNGPKAGQTFHGCNNYPECKHSVW
PK
>Mature_722_residues
MRLWIFEKPSMAKAVIPHLPKPHTRHEGYYETGDGYVSWLAGHVMEQVQPGDYDERWGKFPWAFEDLPIIPKDWKLKVSE
DKKGQVKIIETLLKKCTSIVNGGDADREGQLLVDEVLLHFGNTKPVARIHLSAMDPTSVKKAIASLEDNATFRPLYEAGL
GRQRADWLVGMNMSRAYTILAKKQNYPGVLSVGRVQSPTLALVVKRDREIDGFVPKDFWTVEAQFADPGQSALPFWSRWL
PPGLSLESAEKQADVDAKGEADEDEEGDEIEGEGGTVTGQRPAWLDEQNRIINKAVADQIAANVQAAGQGTVTRYVNKPA
EEQPPLPFELTGLQTALNGKYGYSAKEVLDTCQELYLQGHTTYPRSDCPYLPVSQHAEAPDVLDAIARGAPHLASLAANA
NVTIRSRVWNDSKVTEHHALIPTRQAPDWSTMTEIKRRTYEMIAQRYLAQLYPNCQVDKAKVEVSIAGERFAASGRVVKD
PGWRVVFQGEAQATDAKKAKDATPTLPVLTEQGPVNRLAVRVDAKRTTPPPRYTEGSLLTAMKHVHRTVTDPVERKKLKA
LDGIGRSATRAGIIEMIIKRGFVEVKGKQLVATVVGKILVDALPAKLTDPGMTARWEALLDAIAKKQIPLHAFMAKQEEA
IGELVAAARVSTLPTIPPDAMPARKPYTGSSTPRPAPDGAKKCPKCKKGYLVPRTVKNGPKAGQTFHGCNNYPECKHSVW
PK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=668, Percent_Identity=23.0538922155689, Blast_Score=110, Evalue=7e-24,
Organism=Homo sapiens, GI10835218, Length=666, Percent_Identity=23.7237237237237, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1788061, Length=657, Percent_Identity=38.6605783866058, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32563869, Length=546, Percent_Identity=22.8937728937729, Blast_Score=102, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323263, Length=444, Percent_Identity=25.2252252252252, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585251, Length=717, Percent_Identity=23.5704323570432, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79597; Mature: 79597

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLWIFEKPSMAKAVIPHLPKPHTRHEGYYETGDGYVSWLAGHVMEQVQPGDYDERWGKF
CEEEEECCCCHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCHHHHCCC
PWAFEDLPIIPKDWKLKVSEDKKGQVKIIETLLKKCTSIVNGGDADREGQLLVDEVLLHF
CCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
GNTKPVARIHLSAMDPTSVKKAIASLEDNATFRPLYEAGLGRQRADWLVGMNMSRAYTIL
CCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHCCCCCCCEEEEE
AKKQNYPGVLSVGRVQSPTLALVVKRDREIDGFVPKDFWTVEAQFADPGQSALPFWSRWL
EECCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHCC
PPGLSLESAEKQADVDAKGEADEDEEGDEIEGEGGTVTGQRPAWLDEQNRIINKAVADQI
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHH
AANVQAAGQGTVTRYVNKPAEEQPPLPFELTGLQTALNGKYGYSAKEVLDTCQELYLQGH
HHHHHCCCCCHHHHHHCCCCCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHCCC
TTYPRSDCPYLPVSQHAEAPDVLDAIARGAPHLASLAANANVTIRSRVWNDSKVTEHHAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHCC
IPTRQAPDWSTMTEIKRRTYEMIAQRYLAQLYPNCQVDKAKVEVSIAGERFAASGRVVKD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHCCCCEEEC
PGWRVVFQGEAQATDAKKAKDATPTLPVLTEQGPVNRLAVRVDAKRTTPPPRYTEGSLLT
CCCEEEEECCCCCCCHHHHCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHH
AMKHVHRTVTDPVERKKLKALDGIGRSATRAGIIEMIIKRGFVEVKGKQLVATVVGKILV
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHH
DALPAKLTDPGMTARWEALLDAIAKKQIPLHAFMAKQEEAIGELVAAARVSTLPTIPPDA
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
MPARKPYTGSSTPRPAPDGAKKCPKCKKGYLVPRTVKNGPKAGQTFHGCNNYPECKHSVW
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCEECCHHHCCCCCCCCCCCCCCCCCCCCCCCC
PK
CC
>Mature Secondary Structure
MRLWIFEKPSMAKAVIPHLPKPHTRHEGYYETGDGYVSWLAGHVMEQVQPGDYDERWGKF
CEEEEECCCCHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCHHHHCCC
PWAFEDLPIIPKDWKLKVSEDKKGQVKIIETLLKKCTSIVNGGDADREGQLLVDEVLLHF
CCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
GNTKPVARIHLSAMDPTSVKKAIASLEDNATFRPLYEAGLGRQRADWLVGMNMSRAYTIL
CCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHCCCCCCCEEEEE
AKKQNYPGVLSVGRVQSPTLALVVKRDREIDGFVPKDFWTVEAQFADPGQSALPFWSRWL
EECCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHCC
PPGLSLESAEKQADVDAKGEADEDEEGDEIEGEGGTVTGQRPAWLDEQNRIINKAVADQI
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHH
AANVQAAGQGTVTRYVNKPAEEQPPLPFELTGLQTALNGKYGYSAKEVLDTCQELYLQGH
HHHHHCCCCCHHHHHHCCCCCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHCCC
TTYPRSDCPYLPVSQHAEAPDVLDAIARGAPHLASLAANANVTIRSRVWNDSKVTEHHAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHCC
IPTRQAPDWSTMTEIKRRTYEMIAQRYLAQLYPNCQVDKAKVEVSIAGERFAASGRVVKD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHCCCCEEEC
PGWRVVFQGEAQATDAKKAKDATPTLPVLTEQGPVNRLAVRVDAKRTTPPPRYTEGSLLT
CCCEEEEECCCCCCCHHHHCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHH
AMKHVHRTVTDPVERKKLKALDGIGRSATRAGIIEMIIKRGFVEVKGKQLVATVVGKILV
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHH
DALPAKLTDPGMTARWEALLDAIAKKQIPLHAFMAKQEEAIGELVAAARVSTLPTIPPDA
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
MPARKPYTGSSTPRPAPDGAKKCPKCKKGYLVPRTVKNGPKAGQTFHGCNNYPECKHSVW
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCEECCHHHCCCCCCCCCCCCCCCCCCCCCCCC
PK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]