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The map label for this gene is mdlB [H]

Identifier: 238027837

GI number: 238027837

Start: 2473730

End: 2474902

Strand: Direct

Name: mdlB [H]

Synonym: bglu_1g22680

Alternate gene names: 238027837

Gene position: 2473730-2474902 (Clockwise)

Preceding gene: 238027832

Following gene: 238027842

Centisome position: 63.32

GC content: 75.19

Gene sequence:

>1173_bases
ATGCCCTCACCGATCAACGTCGACGATTTCCGTATGCTGGCCCGCCGCAGGCTGCCGCGCCGCGTGTTCGACTACCTGGA
CGGCGGCGCCGAGGACGAGCGCGGGCTGCGCCGCAATCGCGCCGCGTTCGAGCGGCTCGCGTTCGTGCCGCGGCGGCTTG
CCGACGTCGGCACGCGCGAGCTGTCCACCACGCTGCTCGGCACGCGCCTGGCCGCGCCGTTCGTGATCGCCCCCACCGGG
CTCAACGGCCTGATCCATCCCGACGGCGATCTGGCGCTGGCGCGCGCGGCGCGCCGCGCCGGCATTCCATTCGCGATGTC
CACCGCCTCGAACGTCTCGCTCGAACGGCTCGCGGGGGAGGCGGGCGGCGAGCTGTGGTTTCAGCTCTACGTGATGCACC
GGGAACTGGCCGACAGCCTCGTGCAGCGCGCCGCGCGAGCCGGCTACCGCACGCTGGTGGTGACGGTGGACGTGCCGCTC
AACGGCAAGCGCGAACGCGATCTGCGCAACGGCTTCGCGCTGCCGCTGCGCTGCACGCCGGGCGTGCTGCTCGACGGGCT
CCTGCATCCGCGCTGGTGGTACGCGCTGCTGCGCGGCGGCGGCCTGCCGACGCTCGCCAACCTGGGCGCCGACGGCAACG
CGGGCATCGAGGCGAAAACGGCGCTGCTGCGCCGCCAGATGGATGCGAGCTTCGGCTGGGACGACCTGCGCCGGCTGCGC
GAGCGCTGGCCGCACCGGCTGCTCGTGAAGGGCATCCTCCACACCGGGGACGCCGTGGCCTGCCTCGAGGCGGGCGCCGA
CGGCTTGATCCTCTCGAACCACGGCGCGCGCCAGCTCGACGACGCGGTCGCGCCGCTCGACGTGCTAAGCGCCGCCCGGC
AGGCCTGCGGCGCGCGTGGCGCGCTGCTGGTGGACAGCGGCGTGCGCCGCGGCTCCGACGTGGTGAAGGCGCTCGCGCTC
GGCGCGAACGCGGTGATGCTCGGGCGCGCGACGCTCTACGGGCTCGCCGCGGCCGGCGAGGCGGGCGTGACGCGCGTGCT
CGAGATCCTGCGCGACGAAGTGGATCGCACGCTGGCCATGCTCGGCTGCCGCGGCCTGGCCGAGCTGTCGGCCTCGCATC
TGGCGCCGGCCGCGCCGGCCGCCCCCGCCGCGCCGGGCCGCCAACGCCCCTGA

Upstream 100 bases:

>100_bases
CCCGCCGCGGCGAGCCGTTGCGTATACACTCGTGCGCTCCTGGCGGCCCGGCGGCCCGACTGCCGCCGCATCGTGCCCGA
TCGCCCAGCGCCTCCACGCC

Downstream 100 bases:

>100_bases
CGGCCTGCGCCGCGCCGGCCGATTCCCTCTTGACGCGAACCCGCGGCAGGGCTGACGGCGACCGGGCCGGCCAGGCCGGC
GGGATCACGCCGGCCGCCCC

Product: MdlB

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPSPINVDDFRMLARRRLPRRVFDYLDGGAEDERGLRRNRAAFERLAFVPRRLADVGTRELSTTLLGTRLAAPFVIAPTG
LNGLIHPDGDLALARAARRAGIPFAMSTASNVSLERLAGEAGGELWFQLYVMHRELADSLVQRAARAGYRTLVVTVDVPL
NGKRERDLRNGFALPLRCTPGVLLDGLLHPRWWYALLRGGGLPTLANLGADGNAGIEAKTALLRRQMDASFGWDDLRRLR
ERWPHRLLVKGILHTGDAVACLEAGADGLILSNHGARQLDDAVAPLDVLSAARQACGARGALLVDSGVRRGSDVVKALAL
GANAVMLGRATLYGLAAAGEAGVTRVLEILRDEVDRTLAMLGCRGLAELSASHLAPAAPAAPAAPGRQRP

Sequences:

>Translated_390_residues
MPSPINVDDFRMLARRRLPRRVFDYLDGGAEDERGLRRNRAAFERLAFVPRRLADVGTRELSTTLLGTRLAAPFVIAPTG
LNGLIHPDGDLALARAARRAGIPFAMSTASNVSLERLAGEAGGELWFQLYVMHRELADSLVQRAARAGYRTLVVTVDVPL
NGKRERDLRNGFALPLRCTPGVLLDGLLHPRWWYALLRGGGLPTLANLGADGNAGIEAKTALLRRQMDASFGWDDLRRLR
ERWPHRLLVKGILHTGDAVACLEAGADGLILSNHGARQLDDAVAPLDVLSAARQACGARGALLVDSGVRRGSDVVKALAL
GANAVMLGRATLYGLAAAGEAGVTRVLEILRDEVDRTLAMLGCRGLAELSASHLAPAAPAAPAAPGRQRP
>Mature_389_residues
PSPINVDDFRMLARRRLPRRVFDYLDGGAEDERGLRRNRAAFERLAFVPRRLADVGTRELSTTLLGTRLAAPFVIAPTGL
NGLIHPDGDLALARAARRAGIPFAMSTASNVSLERLAGEAGGELWFQLYVMHRELADSLVQRAARAGYRTLVVTVDVPLN
GKRERDLRNGFALPLRCTPGVLLDGLLHPRWWYALLRGGGLPTLANLGADGNAGIEAKTALLRRQMDASFGWDDLRRLRE
RWPHRLLVKGILHTGDAVACLEAGADGLILSNHGARQLDDAVAPLDVLSAARQACGARGALLVDSGVRRGSDVVKALALG
ANAVMLGRATLYGLAAAGEAGVTRVLEILRDEVDRTLAMLGCRGLAELSASHLAPAAPAAPAAPGRQRP

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=369, Percent_Identity=37.940379403794, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI54234014, Length=370, Percent_Identity=36.7567567567568, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI7705393, Length=370, Percent_Identity=36.7567567567568, Blast_Score=228, Evalue=9e-60,
Organism=Escherichia coli, GI1790033, Length=369, Percent_Identity=37.940379403794, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI193208036, Length=373, Percent_Identity=36.1930294906166, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI32566217, Length=301, Percent_Identity=33.5548172757475, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323587, Length=378, Percent_Identity=33.0687830687831, Blast_Score=203, Evalue=3e-53,
Organism=Drosophila melanogaster, GI78707188, Length=370, Percent_Identity=37.027027027027, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI281363140, Length=370, Percent_Identity=37.027027027027, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI78707190, Length=370, Percent_Identity=37.027027027027, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 41796; Mature: 41665

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPINVDDFRMLARRRLPRRVFDYLDGGAEDERGLRRNRAAFERLAFVPRRLADVGTRE
CCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSTTLLGTRLAAPFVIAPTGLNGLIHPDGDLALARAARRAGIPFAMSTASNVSLERLAGE
HHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHCC
AGGELWFQLYVMHRELADSLVQRAARAGYRTLVVTVDVPLNGKRERDLRNGFALPLRCTP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCEEEEECCC
GVLLDGLLHPRWWYALLRGGGLPTLANLGADGNAGIEAKTALLRRQMDASFGWDDLRRLR
HHHHHHHCCHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
ERWPHRLLVKGILHTGDAVACLEAGADGLILSNHGARQLDDAVAPLDVLSAARQACGARG
HHCCHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
ALLVDSGVRRGSDVVKALALGANAVMLGRATLYGLAAAGEAGVTRVLEILRDEVDRTLAM
CEEECCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LGCRGLAELSASHLAPAAPAAPAAPGRQRP
HHCCHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSPINVDDFRMLARRRLPRRVFDYLDGGAEDERGLRRNRAAFERLAFVPRRLADVGTRE
CCCCCHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSTTLLGTRLAAPFVIAPTGLNGLIHPDGDLALARAARRAGIPFAMSTASNVSLERLAGE
HHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHCC
AGGELWFQLYVMHRELADSLVQRAARAGYRTLVVTVDVPLNGKRERDLRNGFALPLRCTP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHCCCEEEEECCC
GVLLDGLLHPRWWYALLRGGGLPTLANLGADGNAGIEAKTALLRRQMDASFGWDDLRRLR
HHHHHHHCCHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
ERWPHRLLVKGILHTGDAVACLEAGADGLILSNHGARQLDDAVAPLDVLSAARQACGARG
HHCCHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
ALLVDSGVRRGSDVVKALALGANAVMLGRATLYGLAAAGEAGVTRVLEILRDEVDRTLAM
CEEECCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LGCRGLAELSASHLAPAAPAAPAAPGRQRP
HHCCHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]