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The map label for this gene is cobB [H]

Identifier: 238027453

GI number: 238027453

Start: 2033915

End: 2035231

Strand: Direct

Name: cobB [H]

Synonym: bglu_1g18560

Alternate gene names: 238027453

Gene position: 2033915-2035231 (Clockwise)

Preceding gene: 238027452

Following gene: 238027455

Centisome position: 52.06

GC content: 75.09

Gene sequence:

>1317_bases
ATGCCAGCCTGTCCCGCGCTGCTGATCGGCGCGAGCGGGTCCGGCCAGGGCAAGACCTCCGTGACCGCCGGGCTCGCGCG
GCTGCACCGGCGGCTCGGCCGGCGCGTGCGCGTGTTCAAGACCGGCCCGGACTTCCTCGACCCGATGATCCTCGCGCGCG
CGAGCGGCGCCGAGGTCGAGTCGCTCGATCTCGGCATGGTCGGCGAGGCCGGCTGCCGCGCGCTGCTCGCGCACGCGGCG
GCCGAAGCCGACCTGATCCTGATCGAGGGCGTGATGGGGCTCTACGACGGCACGCCGAGCAGCGCCGACCTGGCCTGCCG
GTTCGGCGTGCCGGTGGCCGCGGTGATGTCGGCGACCTCGATGGCGCAGACCTTCGCCGCGCTCGCCTTCGGGCTCGCGC
GGTTTCGCCCCGAGGTGAGGTTTCACGGAGTGCTCGCCAACCGGGTCGGCTCGGCGCGCCATGCCACGCTGCTGCGGGCC
GCGCTGCCCGACGACATCCGCTGGCTCGGTCATCTGCCGACCGATGCCGGCATCGCGCTGCCCGAGCGACACCTCGGGCT
GCACCAGGCCGGCGACATCGCCGATCTCGACACGCGGCTGGAGCGCGCGGCCGACGCGCTCGCGGCCACCGCCCTCGCCG
AGCTGCCGCCGGTGGTCGGCTTCGCGCCGCCCGAGCCGGCGCCGCCGCTGCCGCGCTGGCTCGACGGCCTGCGCGTGGCG
GTGGCGCATGACGCCGCGTTCGCGTTCATCTATCCGGCCAACCTCGCGCTGCTGGCAGCGCTCGGCGCGCGCGTGACGCG
CTTCTCGCCGCTCGCCGACGAGCCGCTGCCCGACTGCGACGCGCTCTATCTGCCGGGCGGCTATCCCGAACTGCATGCGG
CACGCCTGGCCGGCAACGCCCGCAGCGCGGCCGGCATCGCCGCGCACGTGGCGGCCGGCCGGCGCGTGCTGGCCGAATGC
GGCGGGCTGCTCTACCTGTGCGAGACGCTGACCGACGCCAACGGCACGACCACGCCGATGCTCGGGCTGCTGCCGGGCGC
CGCCACCATGCAGCGGCGCTTCACGGCGCTCGGCATGCAACGGCTCGACACGCCGCACGGGCCGCTCAACGGCCATACCT
TCCATTATTCGAAGCTGGAGATGCCGCTCGCGCCGGCCGCGGTGGCCACGCGCCCCGACGACGGCGCGCCCGGCGAGGCG
GTTTACCGGCACGGGCCGATCGTGGCCAGCTACCTGCATGGGTACTGGCCGTCGAACCCGCGCGGCCTTGCGAGCCTCAT
GCTCGGCGAATGGCCCGCGGCCTCGCCGCGTCGCTGA

Upstream 100 bases:

>100_bases
CCGCCGATCTCGTCACCGAGATGCGGCTCGTCAAGCACCCGTACCGCGAGCAGCACGTGAAGGCGCAGCGCGGCGTGGAG
TTCTGATGACGGCGGCCGGC

Downstream 100 bases:

>100_bases
GCCCTCGCCAGGCAGCGATAGCCGCGGCCGACCCGATGCGGCATGCGCCGGCTCAGGCCGAGCCGATCAGCAGGTCGGCC
GGAATCCCGAGCCCCTGGTG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPACPALLIGASGSGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMILARASGAEVESLDLGMVGEAGCRALLAHAA
AEADLILIEGVMGLYDGTPSSADLACRFGVPVAAVMSATSMAQTFAALAFGLARFRPEVRFHGVLANRVGSARHATLLRA
ALPDDIRWLGHLPTDAGIALPERHLGLHQAGDIADLDTRLERAADALAATALAELPPVVGFAPPEPAPPLPRWLDGLRVA
VAHDAAFAFIYPANLALLAALGARVTRFSPLADEPLPDCDALYLPGGYPELHAARLAGNARSAAGIAAHVAAGRRVLAEC
GGLLYLCETLTDANGTTTPMLGLLPGAATMQRRFTALGMQRLDTPHGPLNGHTFHYSKLEMPLAPAAVATRPDDGAPGEA
VYRHGPIVASYLHGYWPSNPRGLASLMLGEWPAASPRR

Sequences:

>Translated_438_residues
MPACPALLIGASGSGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMILARASGAEVESLDLGMVGEAGCRALLAHAA
AEADLILIEGVMGLYDGTPSSADLACRFGVPVAAVMSATSMAQTFAALAFGLARFRPEVRFHGVLANRVGSARHATLLRA
ALPDDIRWLGHLPTDAGIALPERHLGLHQAGDIADLDTRLERAADALAATALAELPPVVGFAPPEPAPPLPRWLDGLRVA
VAHDAAFAFIYPANLALLAALGARVTRFSPLADEPLPDCDALYLPGGYPELHAARLAGNARSAAGIAAHVAAGRRVLAEC
GGLLYLCETLTDANGTTTPMLGLLPGAATMQRRFTALGMQRLDTPHGPLNGHTFHYSKLEMPLAPAAVATRPDDGAPGEA
VYRHGPIVASYLHGYWPSNPRGLASLMLGEWPAASPRR
>Mature_437_residues
PACPALLIGASGSGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMILARASGAEVESLDLGMVGEAGCRALLAHAAA
EADLILIEGVMGLYDGTPSSADLACRFGVPVAAVMSATSMAQTFAALAFGLARFRPEVRFHGVLANRVGSARHATLLRAA
LPDDIRWLGHLPTDAGIALPERHLGLHQAGDIADLDTRLERAADALAATALAELPPVVGFAPPEPAPPLPRWLDGLRVAV
AHDAAFAFIYPANLALLAALGARVTRFSPLADEPLPDCDALYLPGGYPELHAARLAGNARSAAGIAAHVAAGRRVLAECG
GLLYLCETLTDANGTTTPMLGLLPGAATMQRRFTALGMQRLDTPHGPLNGHTFHYSKLEMPLAPAAVATRPDDGAPGEAV
YRHGPIVASYLHGYWPSNPRGLASLMLGEWPAASPRR

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 45776; Mature: 45645

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPACPALLIGASGSGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMILARASGAEVE
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCCE
SLDLGMVGEAGCRALLAHAAAEADLILIEGVMGLYDGTPSSADLACRFGVPVAAVMSATS
ECCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCEEEECCCCHHHHHHHHH
MAQTFAALAFGLARFRPEVRFHGVLANRVGSARHATLLRAALPDDIRWLGHLPTDAGIAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCC
PERHLGLHQAGDIADLDTRLERAADALAATALAELPPVVGFAPPEPAPPLPRWLDGLRVA
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEE
VAHDAAFAFIYPANLALLAALGARVTRFSPLADEPLPDCDALYLPGGYPELHAARLAGNA
EECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCC
RSAAGIAAHVAAGRRVLAECGGLLYLCETLTDANGTTTPMLGLLPGAATMQRRFTALGMQ
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
RLDTPHGPLNGHTFHYSKLEMPLAPAAVATRPDDGAPGEAVYRHGPIVASYLHGYWPSNP
HCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCHHHHCCCHHHHHHHCCCCCCC
RGLASLMLGEWPAASPRR
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PACPALLIGASGSGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMILARASGAEVE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCCE
SLDLGMVGEAGCRALLAHAAAEADLILIEGVMGLYDGTPSSADLACRFGVPVAAVMSATS
ECCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCEEEECCCCHHHHHHHHH
MAQTFAALAFGLARFRPEVRFHGVLANRVGSARHATLLRAALPDDIRWLGHLPTDAGIAL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCC
PERHLGLHQAGDIADLDTRLERAADALAATALAELPPVVGFAPPEPAPPLPRWLDGLRVA
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEE
VAHDAAFAFIYPANLALLAALGARVTRFSPLADEPLPDCDALYLPGGYPELHAARLAGNA
EECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCC
RSAAGIAAHVAAGRRVLAECGGLLYLCETLTDANGTTTPMLGLLPGAATMQRRFTALGMQ
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
RLDTPHGPLNGHTFHYSKLEMPLAPAAVATRPDDGAPGEAVYRHGPIVASYLHGYWPSNP
HCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCHHHHCCCHHHHHHHCCCCCCC
RGLASLMLGEWPAASPRR
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]