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The map label for this gene is nucA [H]

Identifier: 238027417

GI number: 238027417

Start: 1991336

End: 1992088

Strand: Reverse

Name: nucA [H]

Synonym: bglu_1g18130

Alternate gene names: 238027417

Gene position: 1992088-1991336 (Counterclockwise)

Preceding gene: 238027418

Following gene: 238027416

Centisome position: 50.99

GC content: 66.93

Gene sequence:

>753_bases
ATGACGATGAAATGGTTAGCCGCGCTGGGCCTGCTGTCGATCGCGGCGAGCATCCACGCCGCGCCGTGTTCGCAGTTTTC
CCCGGCGGGCCTCGAGCCCGAACTGACGAATCCGAAGATGGTCAGGCAGGCGCGCCTGCTCTGTTATTCCGACTTCGCGG
TGCTGCACTCCGGCATCACGCACACGCCGCTCTGGTCGGCCGAGCATCTGACCGCCGCCCATCTGGCCGACGCCAGGGAC
GAGGTGCGCACCAATCGCTTCTTCGCGGAAACGCGCCTGCCGGCGGGCGAGGGCGCGACGCTGGCCGACTACCGGCGCAG
CGGCTTCGACCGCGGCCACATGAGCCCGGCGGGCGACCGCTGGAACGATGTGGCGATGGCCGAATCGTTCTCGCTGGCGA
ACATGATTCCGCAGAACCCGCAGAACAACCGGCGCCTGTGGGCCCGCGTGGAGGAAGCGGTGCGCGCGATGGCGATGCGC
GACGGCGAGGCCTACGTGGTGACCGGCCCGATGTTCCATGGCAGCGAACTGCAGACGATCGGCGCGAGCCGCGTGATCGT
GCCGACCGAGATCTTCAAGCTGGTCTACCTGCCGGCGCGGCGCGTGGCGTTCGCGATCGTCGTGAAGAATGCCGACGTGA
AGCGCTACGATATCGAAACCGTGCATCAACTGGAGGCCGCCAGCGGGCTGCGTTTCCCCGGCGTGCCGGAACGACTGAAG
GATATGAAGGTAGGAGGGCTGTCCGGTGTCTAA

Upstream 100 bases:

>100_bases
CCGCCGTCAATCCCGAAAAAGATCGACCTGACGTGCTCACCGGCGCCCACGCGGGTGAATCGTCCCGCTACAATGCGCCC
TTTTTTCTCGAAAAAGACGC

Downstream 100 bases:

>100_bases
GACGAATTTCCCCGCATTTGCCGACGACAGCACCGTCGTCAACCTGGCCGGCGACGCGCTGACCATCAGCAACGATCCGT
CGCGCATCCGCGTGAGCGGC

Product: DNA/RNA non-specific endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTMKWLAALGLLSIAASIHAAPCSQFSPAGLEPELTNPKMVRQARLLCYSDFAVLHSGITHTPLWSAEHLTAAHLADARD
EVRTNRFFAETRLPAGEGATLADYRRSGFDRGHMSPAGDRWNDVAMAESFSLANMIPQNPQNNRRLWARVEEAVRAMAMR
DGEAYVVTGPMFHGSELQTIGASRVIVPTEIFKLVYLPARRVAFAIVVKNADVKRYDIETVHQLEAASGLRFPGVPERLK
DMKVGGLSGV

Sequences:

>Translated_250_residues
MTMKWLAALGLLSIAASIHAAPCSQFSPAGLEPELTNPKMVRQARLLCYSDFAVLHSGITHTPLWSAEHLTAAHLADARD
EVRTNRFFAETRLPAGEGATLADYRRSGFDRGHMSPAGDRWNDVAMAESFSLANMIPQNPQNNRRLWARVEEAVRAMAMR
DGEAYVVTGPMFHGSELQTIGASRVIVPTEIFKLVYLPARRVAFAIVVKNADVKRYDIETVHQLEAASGLRFPGVPERLK
DMKVGGLSGV
>Mature_249_residues
TMKWLAALGLLSIAASIHAAPCSQFSPAGLEPELTNPKMVRQARLLCYSDFAVLHSGITHTPLWSAEHLTAAHLADARDE
VRTNRFFAETRLPAGEGATLADYRRSGFDRGHMSPAGDRWNDVAMAESFSLANMIPQNPQNNRRLWARVEEAVRAMAMRD
GEAYVVTGPMFHGSELQTIGASRVIVPTEIFKLVYLPARRVAFAIVVKNADVKRYDIETVHQLEAASGLRFPGVPERLKD
MKVGGLSGV

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI224451073, Length=220, Percent_Identity=29.5454545454545, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI53759134, Length=222, Percent_Identity=32.8828828828829, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI224451075, Length=201, Percent_Identity=29.8507462686567, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17505967, Length=158, Percent_Identity=33.5443037974684, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322253, Length=207, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24581065, Length=208, Percent_Identity=30.2884615384615, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI20129899, Length=160, Percent_Identity=35.625, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28571642, Length=214, Percent_Identity=23.3644859813084, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 27498; Mature: 27367

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKWLAALGLLSIAASIHAAPCSQFSPAGLEPELTNPKMVRQARLLCYSDFAVLHSGIT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
HTPLWSAEHLTAAHLADARDEVRTNRFFAETRLPAGEGATLADYRRSGFDRGHMSPAGDR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
WNDVAMAESFSLANMIPQNPQNNRRLWARVEEAVRAMAMRDGEAYVVTGPMFHGSELQTI
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHC
GASRVIVPTEIFKLVYLPARRVAFAIVVKNADVKRYDIETVHQLEAASGLRFPGVPERLK
CCCEEEEHHHHHHHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHH
DMKVGGLSGV
HCCCCCCCCC
>Mature Secondary Structure 
TMKWLAALGLLSIAASIHAAPCSQFSPAGLEPELTNPKMVRQARLLCYSDFAVLHSGIT
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
HTPLWSAEHLTAAHLADARDEVRTNRFFAETRLPAGEGATLADYRRSGFDRGHMSPAGDR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
WNDVAMAESFSLANMIPQNPQNNRRLWARVEEAVRAMAMRDGEAYVVTGPMFHGSELQTI
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHC
GASRVIVPTEIFKLVYLPARRVAFAIVVKNADVKRYDIETVHQLEAASGLRFPGVPERLK
CCCEEEEHHHHHHHHHCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHH
DMKVGGLSGV
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]