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The map label for this gene is cmlA [H]

Identifier: 238027308

GI number: 238027308

Start: 1882011

End: 1883261

Strand: Direct

Name: cmlA [H]

Synonym: bglu_1g16960

Alternate gene names: 238027308

Gene position: 1882011-1883261 (Clockwise)

Preceding gene: 238027307

Following gene: 238027313

Centisome position: 48.18

GC content: 69.54

Gene sequence:

>1251_bases
ATGAGCCTGCGTTCGAATCCCTCATCGTCCATCGTCTGGATCCTGGTACCCGTCGTCATGCTGGGGCAGCTGTCGATGGA
TCTCTACCTGCCCGCGCTCGATGTGTTGCGCGACGCGCTCGGCGCGACGCCCAACCAGCTGCAGCTAACGCTGAGCGCCT
TCGTGGTCTCGCTGGGTGCCACCCAGTTCGCGATCGGCGAGCTGGCCCGCCGTATCGGCCGTGGCCAAGCGATCCGCTTC
GCGCTGGTGGCCTTCACGCTGGCCACGGCCGGCTGCGCCGCGACCTCGTCGATCGCGCTGTTCATCGCCTGCCGGGTCGT
GCAGGGCGCCGCCGCCGCGATCTGCGTCTCGCTTTCGTATGCGGCGATCGCCGACCAGGCGCGTGACCGCGCCGACAACC
TGCGCGGCACCACGCTGTTGACGGTCGCGATCACGGCCGCACCGCTGGCCGGGCCGGTGGTCGGCTCGCAGGCACTACAG
CTGGGCGGCTCGTGGCGCGCGGTGTTCCTGCTGCTGCTGGCGATCTCGGTGGCGACCATGGTGTTTTTCGCGCGACAGCC
GTTGGCGCGTTTCGGGGTTCGCGGCCTGGATGCCCGGCAGCCTTCGCGCGAGGTCTATCTGCGCATCCTCGGTGCATCGC
GCTTCTGGCGCCACGCGCTGCTCTGCGCGATCGGCACGACCATCAACTTCTGTTTCTTCTCGTTCCTGCCCGGCATCCTG
TTCGGCAGCTACGGTCTGAGCCAGAACGAATTCTCGATCGCGTTCTGTTGCTGCGGCGTGTTCGAGATTGCCGGTTCGCT
GCTCGCGCCTCCCGCCGGCCGCCGCTTCGGCGAGGCCCGCCGCACCCGGCTCGCCTGTGCCTGCGCCTTGCTGGGCGGGC
TCGCGGTGGCCGCCACGGCTAGCCTTTGCACGGCGGTCGTGCCGGTCATCGCGGCCTTTGCGCTGGTGATGTTCGGCACC
GGCATGCTGATCGCGCCGAATTTCGCCGCCGCCTTGTATCCGTTCCGCGAGCATGCGGACCAGGCATCGACGATCTGCAG
CGTGCAGCAGTTCATGATCGCGGCCGGCGTGGCCAGTTGCGCGACGCTGATGCAATGGCAGTCGGCTTCGGCGCTCGGCT
TGCTGATCGCCGCCGTCGCGACGCTGGCCCTCGCCATCGGCTTCGCGCCGATGGTTGTCGCCCCCGAGGCCGGCCTGCCG
GGCGGCGCCGAGCAGCCGGTGGCGAGCCGGGGCGGTGAGGTTTCCGGGTGA

Upstream 100 bases:

>100_bases
TCTGCCCGGGACCGCCGGGTCCGGCCGTGCATCGCAGGCCGGGCCCGGCCACCCGCTCTCCCATCCGCCTGCCTCCTTTC
CCATCACGAGACGCGTTCCC

Downstream 100 bases:

>100_bases
GTGAAGCGGAGCCGCCAAGGCGGGCGAGGTGAGGCGCGGCGCCGGCTGCTTGCCGCGGCTCGACGAGTCGGGCGCCGGGC
CAGCGCAGGCCGCCAGGCTG

Product: putative transport/efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSLRSNPSSSIVWILVPVVMLGQLSMDLYLPALDVLRDALGATPNQLQLTLSAFVVSLGATQFAIGELARRIGRGQAIRF
ALVAFTLATAGCAATSSIALFIACRVVQGAAAAICVSLSYAAIADQARDRADNLRGTTLLTVAITAAPLAGPVVGSQALQ
LGGSWRAVFLLLLAISVATMVFFARQPLARFGVRGLDARQPSREVYLRILGASRFWRHALLCAIGTTINFCFFSFLPGIL
FGSYGLSQNEFSIAFCCCGVFEIAGSLLAPPAGRRFGEARRTRLACACALLGGLAVAATASLCTAVVPVIAAFALVMFGT
GMLIAPNFAAALYPFREHADQASTICSVQQFMIAAGVASCATLMQWQSASALGLLIAAVATLALAIGFAPMVVAPEAGLP
GGAEQPVASRGGEVSG

Sequences:

>Translated_416_residues
MSLRSNPSSSIVWILVPVVMLGQLSMDLYLPALDVLRDALGATPNQLQLTLSAFVVSLGATQFAIGELARRIGRGQAIRF
ALVAFTLATAGCAATSSIALFIACRVVQGAAAAICVSLSYAAIADQARDRADNLRGTTLLTVAITAAPLAGPVVGSQALQ
LGGSWRAVFLLLLAISVATMVFFARQPLARFGVRGLDARQPSREVYLRILGASRFWRHALLCAIGTTINFCFFSFLPGIL
FGSYGLSQNEFSIAFCCCGVFEIAGSLLAPPAGRRFGEARRTRLACACALLGGLAVAATASLCTAVVPVIAAFALVMFGT
GMLIAPNFAAALYPFREHADQASTICSVQQFMIAAGVASCATLMQWQSASALGLLIAAVATLALAIGFAPMVVAPEAGLP
GGAEQPVASRGGEVSG
>Mature_415_residues
SLRSNPSSSIVWILVPVVMLGQLSMDLYLPALDVLRDALGATPNQLQLTLSAFVVSLGATQFAIGELARRIGRGQAIRFA
LVAFTLATAGCAATSSIALFIACRVVQGAAAAICVSLSYAAIADQARDRADNLRGTTLLTVAITAAPLAGPVVGSQALQL
GGSWRAVFLLLLAISVATMVFFARQPLARFGVRGLDARQPSREVYLRILGASRFWRHALLCAIGTTINFCFFSFLPGILF
GSYGLSQNEFSIAFCCCGVFEIAGSLLAPPAGRRFGEARRTRLACACALLGGLAVAATASLCTAVVPVIAAFALVMFGTG
MLIAPNFAAALYPFREHADQASTICSVQQFMIAAGVASCATLMQWQSASALGLLIAAVATLALAIGFAPMVVAPEAGLPG
GAEQPVASRGGEVSG

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=323, Percent_Identity=28.4829721362229, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788509, Length=394, Percent_Identity=27.1573604060914, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43041; Mature: 42910

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00213 LIPOCALIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRSNPSSSIVWILVPVVMLGQLSMDLYLPALDVLRDALGATPNQLQLTLSAFVVSLGA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
TQFAIGELARRIGRGQAIRFALVAFTLATAGCAATSSIALFIACRVVQGAAAAICVSLSY
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIADQARDRADNLRGTTLLTVAITAAPLAGPVVGSQALQLGGSWRAVFLLLLAISVATM
HHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCHHCHHHHHCCCCHHHHHHHHHHHHHHHH
VFFARQPLARFGVRGLDARQPSREVYLRILGASRFWRHALLCAIGTTINFCFFSFLPGIL
HHHHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSYGLSQNEFSIAFCCCGVFEIAGSLLAPPAGRRFGEARRTRLACACALLGGLAVAATA
HHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SLCTAVVPVIAAFALVMFGTGMLIAPNFAAALYPFREHADQASTICSVQQFMIAAGVASC
HHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLMQWQSASALGLLIAAVATLALAIGFAPMVVAPEAGLPGGAEQPVASRGGEVSG
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
SLRSNPSSSIVWILVPVVMLGQLSMDLYLPALDVLRDALGATPNQLQLTLSAFVVSLGA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
TQFAIGELARRIGRGQAIRFALVAFTLATAGCAATSSIALFIACRVVQGAAAAICVSLSY
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIADQARDRADNLRGTTLLTVAITAAPLAGPVVGSQALQLGGSWRAVFLLLLAISVATM
HHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCHHCHHHHHCCCCHHHHHHHHHHHHHHHH
VFFARQPLARFGVRGLDARQPSREVYLRILGASRFWRHALLCAIGTTINFCFFSFLPGIL
HHHHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGSYGLSQNEFSIAFCCCGVFEIAGSLLAPPAGRRFGEARRTRLACACALLGGLAVAATA
HHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SLCTAVVPVIAAFALVMFGTGMLIAPNFAAALYPFREHADQASTICSVQQFMIAAGVASC
HHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLMQWQSASALGLLIAAVATLALAIGFAPMVVAPEAGLPGGAEQPVASRGGEVSG
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]