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The map label for this gene is potI [H]

Identifier: 238027225

GI number: 238027225

Start: 1763605

End: 1764423

Strand: Direct

Name: potI [H]

Synonym: bglu_1g16110

Alternate gene names: 238027225

Gene position: 1763605-1764423 (Clockwise)

Preceding gene: 238027224

Following gene: 238027228

Centisome position: 45.15

GC content: 66.06

Gene sequence:

>819_bases
ATGATGCCGAATCGTTATCTGCGATTGCTCGCGCTGGCGGTCGGCTTCGCGTTCCTCTACATCCCGATCCTGAGCCTCGT
CGTCTACTCGTTCAACGAGTCCCAGCTGGTCACGGTCTGGTCCGGCTTCTCGACGCGCTGGTACGCGGCGCTGCTTGGCG
ACGACGAACTGATCGCGGCCGCCTGGCTGTCGCTGAAGATTGGCATTCTGACGGCGTTCGCCTCGGTGGCGATCGGCACC
TGGGCCGGCTACGTGCTGGCGCGCATGGGGCGCTTCCGCGGTTTCGCGCTTTATTCCGGGATGATCAACGCGCCGCTGGT
GATCCCCGAGGTGATCCAGGGCATCTCGCTGCTGCTGCTGTTCATCGAGCTGGCGAAGTGGATCGGCTGGCCGGCGCAGC
GCGGCATGCTGACGATCTGGCTCGGCCACGTGATGCTGTGCCTGTCCTATGTCGCGATCATCGTGCAGTCGCGCGTGCGC
GAACTCGATCCGTCGCTGGAGGAGGCCGCGCTCGATCTCGGCGCCACGCCGCTGAAGGTGTTCTTCACGATCACGCTGCC
GCTGATCTCGCAGGCGCTGGTGTCGGGCTGGCTGCTGTCGTTTACGCTGTCGATCGACGATCTGGTGCTGTCCGCGTTCC
TGTCCGGCCCCGGCTCGACCACGCTGCCGCTGGTGGTGTTCTCGCGCGTGCGGCTCGGCCTGAATCCGGAAATGAACGCA
CTCGCGACGCTGTTCATCGTCGCCGTGACGATCGGCGTGATCGGCGCGAACATGATGATGCTGCGCCGCGAGCGGCGCCG
GCTCGCGGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
CGATGGCCTCGGCCGTGACCTGCGCGATGGTGCTGCTGCTGCTCGTGCCGATGGCGGTCTTCCAGCATTACCAGGCCAAG
GAAGCGGAGGGCCGTCGCAA

Downstream 100 bases:

>100_bases
GCGTGGGCGGGCCACACGCCCCGCCCACTTGCCGATCGCGACGCCCCGCATCGTCGCGCGCCGCGCCCCTGCATCGGGCC
CCGCGCGAGCGCCTGAGGCC

Product: putrescine ABC transporter permease PotI

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MMPNRYLRLLALAVGFAFLYIPILSLVVYSFNESQLVTVWSGFSTRWYAALLGDDELIAAAWLSLKIGILTAFASVAIGT
WAGYVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAQRGMLTIWLGHVMLCLSYVAIIVQSRVR
ELDPSLEEAALDLGATPLKVFFTITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVIGANMMMLRRERRRLAAAA

Sequences:

>Translated_272_residues
MMPNRYLRLLALAVGFAFLYIPILSLVVYSFNESQLVTVWSGFSTRWYAALLGDDELIAAAWLSLKIGILTAFASVAIGT
WAGYVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAQRGMLTIWLGHVMLCLSYVAIIVQSRVR
ELDPSLEEAALDLGATPLKVFFTITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVIGANMMMLRRERRRLAAAA
>Mature_272_residues
MMPNRYLRLLALAVGFAFLYIPILSLVVYSFNESQLVTVWSGFSTRWYAALLGDDELIAAAWLSLKIGILTAFASVAIGT
WAGYVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLLFIELAKWIGWPAQRGMLTIWLGHVMLCLSYVAIIVQSRVR
ELDPSLEEAALDLGATPLKVFFTITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
LATLFIVAVTIGVIGANMMMLRRERRRLAAAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=263, Percent_Identity=60.8365019011407, Blast_Score=290, Evalue=5e-80,
Organism=Escherichia coli, GI1787368, Length=240, Percent_Identity=38.3333333333333, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787714, Length=254, Percent_Identity=31.1023622047244, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788764, Length=232, Percent_Identity=28.8793103448276, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29782; Mature: 29782

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPNRYLRLLALAVGFAFLYIPILSLVVYSFNESQLVTVWSGFSTRWYAALLGDDELIAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHEECCCCHHHHH
AWLSLKIGILTAFASVAIGTWAGYVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FIELAKWIGWPAQRGMLTIWLGHVMLCLSYVAIIVQSRVRELDPSLEEAALDLGATPLKV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFTITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIVAVTIGVIGANMMMLRRERRRLAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMPNRYLRLLALAVGFAFLYIPILSLVVYSFNESQLVTVWSGFSTRWYAALLGDDELIAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHEECCCCHHHHH
AWLSLKIGILTAFASVAIGTWAGYVLARMGRFRGFALYSGMINAPLVIPEVIQGISLLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FIELAKWIGWPAQRGMLTIWLGHVMLCLSYVAIIVQSRVRELDPSLEEAALDLGATPLKV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
FFTITLPLISQALVSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
LATLFIVAVTIGVIGANMMMLRRERRRLAAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]