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The map label for this gene is pit [H]

Identifier: 238027022

GI number: 238027022

Start: 1540427

End: 1541437

Strand: Direct

Name: pit [H]

Synonym: bglu_1g14000

Alternate gene names: 238027022

Gene position: 1540427-1541437 (Clockwise)

Preceding gene: 238027021

Following gene: 238027036

Centisome position: 39.43

GC content: 66.17

Gene sequence:

>1011_bases
ATGCATTCGATACAACTCGCCATCTGGGCCGTCGCGACGCTCGTGATCGTCGCGCTCGTGTTCGACTTCATGAACGGCTT
CCACGACGCGGCGAACTCGATCGCCACCGTCGTTTCCACCGGCGTGCTGAAGCCGCAGCAGGCGGTGGCATTCGCCGCCG
TGTTCAACGTGGTCGCCTATTTCGTGTTCCACCTGAAGGTCGCGCAGACCGTGGGCAAGGGCACCATCGATCCCGCCATC
GTCGATCATTACGTGGTGTTCGGCGCGCTGGTCGGCGCGATCGGCTGGAACGTCATCACCTGGTATTACGGGATTCCGTC
GAGTTCGTCGCATGCGCTGATCGGCGGGCTGGTCGGCGCGGCCGTCGCGAAATCGGGCTGGGGCTCGCTGAATCTCGACG
GTCTGATGAAGACGGTGGCCTTCATTTTCGTCTCGCCGCTGCTCGGTTTCGTGCTCGGCTCGCTGTTCATGCTCGTGGTG
TCCTGGTTCTATTTCCGCACGCCGCCGAGCAGGGTGGACCGGCGCTTCCGCAAGCTGCAACTGGTCTCGGCCGGGCTCTA
CAGCCTCGGCCACGGCGGCAACGACGCACAGAAGACGATCGGCATGATCTGGATGCTGCTGATCGCGACCGGCTACGCCT
CGGCCGGCGCCGATGCGCCGCCCATCTGGGTGATCGGCGCCTGCTACCTGTCGATGGGGCTCGGCACGCTGTTCGGCGGC
TGGCGCATCGTGCGCACGATGGGGCAGAAGATCACCAAGCTCAAGCCGGTCGGCGGGTTCTGCGCCGAGCTGGGCGGGGC
GATCACGCTGTTCTCGGCGTCCTGGCTCGGCATCCCCGTGTCGACCACGCACACCATCACGGGCGCGATCGTCGGCGTGG
GCGCGACGCGCAAGCTGTCGGCCGTGCGTTGGGGCGTGGCCGGTAATATCGTGTGGGCCTGGGTGCTGACGATTCCGGCC
TCGGCGCTGATCGCAGCGGCCGGCTGGTGGGTGGGGCACCAGGTGTTTTGA

Upstream 100 bases:

>100_bases
AAGGCGATCTACGAACTGCTCGAGGAAATCACCGACAAGTGCGAGGACGTGGCGAACATCATCGAAGGCATCGTGCTGGA
AAACGCCTGAGCCGACGACG

Downstream 100 bases:

>100_bases
TGCGTTGGACCGGCGGCTGTTGCCGCCGGATTCAGCGGGATGGTGATGTCGCGCCGCGTGCCGATGGCCGCGGCGCGCTT
TGCTTTGGGCGGCCGCGGGC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MHSIQLAIWAVATLVIVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAVFNVVAYFVFHLKVAQTVGKGTIDPAI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAAVAKSGWGSLNLDGLMKTVAFIFVSPLLGFVLGSLFMLVV
SWFYFRTPPSRVDRRFRKLQLVSAGLYSLGHGGNDAQKTIGMIWMLLIATGYASAGADAPPIWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAELGGAITLFSASWLGIPVSTTHTITGAIVGVGATRKLSAVRWGVAGNIVWAWVLTIPA
SALIAAAGWWVGHQVF

Sequences:

>Translated_336_residues
MHSIQLAIWAVATLVIVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAVFNVVAYFVFHLKVAQTVGKGTIDPAI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAAVAKSGWGSLNLDGLMKTVAFIFVSPLLGFVLGSLFMLVV
SWFYFRTPPSRVDRRFRKLQLVSAGLYSLGHGGNDAQKTIGMIWMLLIATGYASAGADAPPIWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAELGGAITLFSASWLGIPVSTTHTITGAIVGVGATRKLSAVRWGVAGNIVWAWVLTIPA
SALIAAAGWWVGHQVF
>Mature_336_residues
MHSIQLAIWAVATLVIVALVFDFMNGFHDAANSIATVVSTGVLKPQQAVAFAAVFNVVAYFVFHLKVAQTVGKGTIDPAI
VDHYVVFGALVGAIGWNVITWYYGIPSSSSHALIGGLVGAAVAKSGWGSLNLDGLMKTVAFIFVSPLLGFVLGSLFMLVV
SWFYFRTPPSRVDRRFRKLQLVSAGLYSLGHGGNDAQKTIGMIWMLLIATGYASAGADAPPIWVIGACYLSMGLGTLFGG
WRIVRTMGQKITKLKPVGGFCAELGGAITLFSASWLGIPVSTTHTITGAIVGVGATRKLSAVRWGVAGNIVWAWVLTIPA
SALIAAAGWWVGHQVF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=173, Percent_Identity=38.150289017341, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI5803173, Length=159, Percent_Identity=37.1069182389937, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789907, Length=225, Percent_Identity=32.8888888888889, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789360, Length=239, Percent_Identity=32.2175732217573, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=32.0987654320988, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17539280, Length=179, Percent_Identity=33.5195530726257, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71981576, Length=163, Percent_Identity=31.2883435582822, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI32566716, Length=169, Percent_Identity=29.585798816568, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536725, Length=172, Percent_Identity=31.9767441860465, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17557328, Length=162, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319773, Length=158, Percent_Identity=32.9113924050633, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356511, Length=182, Percent_Identity=31.3186813186813, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35515; Mature: 35515

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]