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The map label for this gene is icaA [H]

Identifier: 238026728

GI number: 238026728

Start: 1188858

End: 1190405

Strand: Reverse

Name: icaA [H]

Synonym: bglu_1g10850

Alternate gene names: 238026728

Gene position: 1190405-1188858 (Counterclockwise)

Preceding gene: 238026729

Following gene: 238026724

Centisome position: 30.47

GC content: 72.8

Gene sequence:

>1548_bases
GTGAGCACGACCTCGCCCGCCCCGAACCGGGAGCCTCGCCAGGGCCATCCGCGCCGCCTCGCCGCGAGCCGCGCCCATGC
CGTCACCGCCGCCGCGCGCGACGCGCTCGATCTGGCCCGCACGCGCCTGTCGCCGCGCCTCACGCCGCGCGCCGCGCCGC
TGGCGAGCCGGCTGATCCACGGCGGCGCGCTGCTGCTCTGGATCGCGCTGTTCGCGCGCGCGCTCGCACCGGGCCTGGCG
TCGTGGTCGGTGGGCATCGCCTACGTGATCTACGACACGCTGCTGCTGAGCTTCGTGGCCGTCAAGATCCTGCCGCTCGC
CGGGCCGCTCGCGCGCGTCCACGCCCCGGCCGCCCCCGCGCCCGGCACGCCTCGCCCGTCGCTCGGCGTGGTGGTGGCCG
CCTATAACGAGGCCCCCGTGATCCAGGTCACGCTCGACGGTCTGTTCGCGCAGACCGAGCCGCTGCGGCAGATCGTGTTC
GCCGACGACGGCTCGACCGATGCCACCATCGAGGTGCTGACCACCCGGTACGGCCTGGTGCAGGCGCCCGACGGCGAGCT
GAGCGCGCCCAGCACGCGCCACCCGAACCTGCGCTGGCTGCGCGTGCCGCATGGCGGCAAGGCGGCGGCGCTGAACGCCG
CGCTGCTCCACCTCGACACCGACGCGGTGCTGACCGTGGACGCCGACACGCGGCTCGCCGCCGACGCCTGCGCCGCGATG
CGCCGCGCGTTCGCGGCCGAGCCGGCGCTGGTGGCCGCCACCGGCATCCTCACGCCGATCTGCGCGCGCGACCTGTCGGG
CCGCTTCTTCCAGTGGTTCCAGACCTACGAGTACATCCGCAACTTCATCTCGCGCTTCGCCTGGATGCGCGCCGACAGCC
TGCTGCTGATCTCGGGCGCGTTCGCCTGCTTCCGGCGCGACGCGCTGCTGAAGGTGGGCGGCTTCGACGGCGCCTGCCTG
GTGGAGGATTACGAACTGATTCACCGGCTGCGCCGCTACGCGGTGGAGAACGGCGAGACCTGGCGCGTGCGCGTGATCGG
CGACGCCCACGCACGCACCGACGCGCCCGGCACGCTGCGCAGCTTCCTGCGCCAGCGCCGCCGCTGGTTCGCCGGCTTCC
TGCAGACGCAGTACTGGAACCGCGACATGACCGGCAACCGCCGCTACGGCCAGCTCGGCCTGCTGATGCTGCCCGTCAAG
GCGCTCGACACGCTGCAGCCGATCTACGGGCTCACCGCGATCGTGCTGCTGATGCTGTTCCTCTGCCAGCACCGCTTCCA
CGTCGCGCTGTCGATCTCGGGCGTGATCGCGGCGAAGATCGTGATCGACTTCGCGTTCCATCTCTGGTCGATCCACCTGT
ACCGCCGCTGGACCGGCAGCCGCGAGCATTCGAGCCTCGCGATCGCGTTCGTGGCCTCGGTGCTCGAGCCGTTCAGCTTC
CAGGTGTTGCGCCACGCCGGCGCCGCGCTCGGCTGGATCCACTTCCTGCGCGGCAACCAGGGCTGGGGCGCCCAGGAGCG
CACCGGGCTGGTCGGCGACGGACCATGA

Upstream 100 bases:

>100_bases
GGGGCGCCTACGCGCTCTACGCGGTGCTGGCGATGCATATCCTCGGCGCGCTCAAGCACGAGCTGCTCGATCGCGAATCG
GTCCTGCGCCGCATGCTGCC

Downstream 100 bases:

>100_bases
GAGCGCGGCGGCAAGGCGGCCGCGCCCCGCCCGGGGCCGCTCAGCGCCCCGCGCCGCCGCCCAGCCGCACCGAGCGGTAG
GTCTGCGCGCGCTCGGGATG

Product: family 2 glycosyl transferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSTTSPAPNREPRQGHPRRLAASRAHAVTAAARDALDLARTRLSPRLTPRAAPLASRLIHGGALLLWIALFARALAPGLA
SWSVGIAYVIYDTLLLSFVAVKILPLAGPLARVHAPAAPAPGTPRPSLGVVVAAYNEAPVIQVTLDGLFAQTEPLRQIVF
ADDGSTDATIEVLTTRYGLVQAPDGELSAPSTRHPNLRWLRVPHGGKAAALNAALLHLDTDAVLTVDADTRLAADACAAM
RRAFAAEPALVAATGILTPICARDLSGRFFQWFQTYEYIRNFISRFAWMRADSLLLISGAFACFRRDALLKVGGFDGACL
VEDYELIHRLRRYAVENGETWRVRVIGDAHARTDAPGTLRSFLRQRRRWFAGFLQTQYWNRDMTGNRRYGQLGLLMLPVK
ALDTLQPIYGLTAIVLLMLFLCQHRFHVALSISGVIAAKIVIDFAFHLWSIHLYRRWTGSREHSSLAIAFVASVLEPFSF
QVLRHAGAALGWIHFLRGNQGWGAQERTGLVGDGP

Sequences:

>Translated_515_residues
MSTTSPAPNREPRQGHPRRLAASRAHAVTAAARDALDLARTRLSPRLTPRAAPLASRLIHGGALLLWIALFARALAPGLA
SWSVGIAYVIYDTLLLSFVAVKILPLAGPLARVHAPAAPAPGTPRPSLGVVVAAYNEAPVIQVTLDGLFAQTEPLRQIVF
ADDGSTDATIEVLTTRYGLVQAPDGELSAPSTRHPNLRWLRVPHGGKAAALNAALLHLDTDAVLTVDADTRLAADACAAM
RRAFAAEPALVAATGILTPICARDLSGRFFQWFQTYEYIRNFISRFAWMRADSLLLISGAFACFRRDALLKVGGFDGACL
VEDYELIHRLRRYAVENGETWRVRVIGDAHARTDAPGTLRSFLRQRRRWFAGFLQTQYWNRDMTGNRRYGQLGLLMLPVK
ALDTLQPIYGLTAIVLLMLFLCQHRFHVALSISGVIAAKIVIDFAFHLWSIHLYRRWTGSREHSSLAIAFVASVLEPFSF
QVLRHAGAALGWIHFLRGNQGWGAQERTGLVGDGP
>Mature_514_residues
STTSPAPNREPRQGHPRRLAASRAHAVTAAARDALDLARTRLSPRLTPRAAPLASRLIHGGALLLWIALFARALAPGLAS
WSVGIAYVIYDTLLLSFVAVKILPLAGPLARVHAPAAPAPGTPRPSLGVVVAAYNEAPVIQVTLDGLFAQTEPLRQIVFA
DDGSTDATIEVLTTRYGLVQAPDGELSAPSTRHPNLRWLRVPHGGKAAALNAALLHLDTDAVLTVDADTRLAADACAAMR
RAFAAEPALVAATGILTPICARDLSGRFFQWFQTYEYIRNFISRFAWMRADSLLLISGAFACFRRDALLKVGGFDGACLV
EDYELIHRLRRYAVENGETWRVRVIGDAHARTDAPGTLRSFLRQRRRWFAGFLQTQYWNRDMTGNRRYGQLGLLMLPVKA
LDTLQPIYGLTAIVLLMLFLCQHRFHVALSISGVIAAKIVIDFAFHLWSIHLYRRWTGSREHSSLAIAFVASVLEPFSFQ
VLRHAGAALGWIHFLRGNQGWGAQERTGLVGDGP

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=310, Percent_Identity=24.8387096774194, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 56461; Mature: 56330

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTSPAPNREPRQGHPRRLAASRAHAVTAAARDALDLARTRLSPRLTPRAAPLASRLIH
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GGALLLWIALFARALAPGLASWSVGIAYVIYDTLLLSFVAVKILPLAGPLARVHAPAAPA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
PGTPRPSLGVVVAAYNEAPVIQVTLDGLFAQTEPLRQIVFADDGSTDATIEVLTTRYGLV
CCCCCCCCCEEEEEECCCCEEEEEECCHHHCCHHHHEEEEECCCCCCCEEEEEEECCCEE
QAPDGELSAPSTRHPNLRWLRVPHGGKAAALNAALLHLDTDAVLTVDADTRLAADACAAM
ECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHEEEEEECCCEEEEECCCCHHHHHHHHHH
RRAFAAEPALVAATGILTPICARDLSGRFFQWFQTYEYIRNFISRFAWMRADSLLLISGA
HHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
FACFRRDALLKVGGFDGACLVEDYELIHRLRRYAVENGETWRVRVIGDAHARTDAPGTLR
HHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHH
SFLRQRRRWFAGFLQTQYWNRDMTGNRRYGQLGLLMLPVKALDTLQPIYGLTAIVLLMLF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
LCQHRFHVALSISGVIAAKIVIDFAFHLWSIHLYRRWTGSREHSSLAIAFVASVLEPFSF
HHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QVLRHAGAALGWIHFLRGNQGWGAQERTGLVGDGP
HHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STTSPAPNREPRQGHPRRLAASRAHAVTAAARDALDLARTRLSPRLTPRAAPLASRLIH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
GGALLLWIALFARALAPGLASWSVGIAYVIYDTLLLSFVAVKILPLAGPLARVHAPAAPA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
PGTPRPSLGVVVAAYNEAPVIQVTLDGLFAQTEPLRQIVFADDGSTDATIEVLTTRYGLV
CCCCCCCCCEEEEEECCCCEEEEEECCHHHCCHHHHEEEEECCCCCCCEEEEEEECCCEE
QAPDGELSAPSTRHPNLRWLRVPHGGKAAALNAALLHLDTDAVLTVDADTRLAADACAAM
ECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHEEEEEECCCEEEEECCCCHHHHHHHHHH
RRAFAAEPALVAATGILTPICARDLSGRFFQWFQTYEYIRNFISRFAWMRADSLLLISGA
HHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
FACFRRDALLKVGGFDGACLVEDYELIHRLRRYAVENGETWRVRVIGDAHARTDAPGTLR
HHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHH
SFLRQRRRWFAGFLQTQYWNRDMTGNRRYGQLGLLMLPVKALDTLQPIYGLTAIVLLMLF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
LCQHRFHVALSISGVIAAKIVIDFAFHLWSIHLYRRWTGSREHSSLAIAFVASVLEPFSF
HHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QVLRHAGAALGWIHFLRGNQGWGAQERTGLVGDGP
HHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA