Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 238026545

GI number: 238026545

Start: 966903

End: 968183

Strand: Reverse

Name: mltB [H]

Synonym: bglu_1g08890

Alternate gene names: 238026545

Gene position: 968183-966903 (Counterclockwise)

Preceding gene: 238026547

Following gene: 238026528

Centisome position: 24.78

GC content: 69.71

Gene sequence:

>1281_bases
ATGACTTTCCGATCGTCCGCCGTCTTCCATCCGCTTCGTTTCCGCTCACGTTTGCCGCTCGCGTCGCTCTCGCTCGCGGC
CGTGGTCGCGACGCTGGCCGCATGCGCGCAGACGCCGACCGAGCCGCAGGCCGCAGCGCCGGCAACGCCCACTGCGCCGG
TCGCGCCCGCTGCCGTGCCTGACGCCCCTGCCTCCGCTTCCGCGCCGGCCCCTGCCTCCGCCCCCGCCGCCGCGCCGGCC
GCGGCCACCGCGCCCCAACTGGTGCCGGCCGCCGGGCTCTCGGCCTCCGAAGAGCAGATCGTGCCGCAGCAATACGCGAA
CAATCCGAAGATCGACGCGTTCATCGACGAAGTGGCCGCGCGCGACGGCTTCGATCCGGCGAAGCTGCATGCCTTGTTCG
CGAGTGCGAACTATTCGGCGACGGCAGTGCGGCTGGTGCGCCCCGCCCGGTCGCCGTCGGTCAAGAACTGGCGCGCCTAT
CAGGCGCGCTTTCTCGATGCGATCCGCATCAACGCGGGCGTGAAGTTCTGGCGCGCGAACCAGGCGACGCTGCAGCGCGC
GTCGGCCGAGTTCGGCGTGCCGCCCGAGGTGATCGTCGGCATCATCGGCGTCGAGACGATCTACGGCCGCTACATGGGCA
ACTTCCCGACGCTCGACGCGCTGACGACGCTCGCGTTCGACTATCCCGACACGCCGAACCGCGACGACCGGCAGGCCACC
TTCCGCAAGAACCTCGAGGATTTCCTGGTCTGGACGCGCGACAGCCAGCTCGATCCGACCAGCGTGCTCGGTTCGTACAC
CGGTGCGATCGGCATTCCGCAGTTCCTGCCGAGCAGCATTCGCAGCTACGCGGTCGACTACGAGGGCAACGGCCACATCG
ACCTGCGCGCGAGCACCGCCGACGCGATCGGCAGCGTCGCGAACTATCTGAAGCAGCACGGCTGGGAGCCGGGCCGCCCG
GTGGTATGGCAGATCGCGCCGGACGCGGGCAGCCAGGGTGTCGCGCAGGCCGCGGCCGACGGCAAGCCCGAGCCGCACCT
CAAGCTGTCGCAGCTCACCCAGGCCGGCCTGGTGCTCGACGAGCCGTCGGTGGACCCGGCCGCCGAGGCCGGCACGCTGG
TCACGGTCGTCGATCTGCCTAGCCCGGGGCGCGCGACCGAGTACAAGCTCGGCCTGAAGAACTTCTATGTGCTGACACGC
TACAACCGCAGCTTCTTCTACGCGCTGGCCGTCTATCAGCTCGGCGAGCGCGTGAAGCAAAAGATGCTCGCCACGCAGTG
A

Upstream 100 bases:

>100_bases
TCGCCGCTAACTTACCACAACGGCCTGCGTCGATCCGTCGCCTTAGAGCCCTCGGTTATACTGGACCGACTTCCACCGCG
TCGTCCACCCTCCGGTACCC

Downstream 100 bases:

>100_bases
GGCCCAGGCACCTCGCCGGACCCATGAAAAAAGCGCCCTCGTGGCGCTTTTTTCTTGGGCGAAGACGGCAGCGAGCCGCT
TGCGGCACTCAGGCGGGGAA

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTFRSSAVFHPLRFRSRLPLASLSLAAVVATLAACAQTPTEPQAAAPATPTAPVAPAAVPDAPASASAPAPASAPAAAPA
AATAPQLVPAAGLSASEEQIVPQQYANNPKIDAFIDEVAARDGFDPAKLHALFASANYSATAVRLVRPARSPSVKNWRAY
QARFLDAIRINAGVKFWRANQATLQRASAEFGVPPEVIVGIIGVETIYGRYMGNFPTLDALTTLAFDYPDTPNRDDRQAT
FRKNLEDFLVWTRDSQLDPTSVLGSYTGAIGIPQFLPSSIRSYAVDYEGNGHIDLRASTADAIGSVANYLKQHGWEPGRP
VVWQIAPDAGSQGVAQAAADGKPEPHLKLSQLTQAGLVLDEPSVDPAAEAGTLVTVVDLPSPGRATEYKLGLKNFYVLTR
YNRSFFYALAVYQLGERVKQKMLATQ

Sequences:

>Translated_426_residues
MTFRSSAVFHPLRFRSRLPLASLSLAAVVATLAACAQTPTEPQAAAPATPTAPVAPAAVPDAPASASAPAPASAPAAAPA
AATAPQLVPAAGLSASEEQIVPQQYANNPKIDAFIDEVAARDGFDPAKLHALFASANYSATAVRLVRPARSPSVKNWRAY
QARFLDAIRINAGVKFWRANQATLQRASAEFGVPPEVIVGIIGVETIYGRYMGNFPTLDALTTLAFDYPDTPNRDDRQAT
FRKNLEDFLVWTRDSQLDPTSVLGSYTGAIGIPQFLPSSIRSYAVDYEGNGHIDLRASTADAIGSVANYLKQHGWEPGRP
VVWQIAPDAGSQGVAQAAADGKPEPHLKLSQLTQAGLVLDEPSVDPAAEAGTLVTVVDLPSPGRATEYKLGLKNFYVLTR
YNRSFFYALAVYQLGERVKQKMLATQ
>Mature_425_residues
TFRSSAVFHPLRFRSRLPLASLSLAAVVATLAACAQTPTEPQAAAPATPTAPVAPAAVPDAPASASAPAPASAPAAAPAA
ATAPQLVPAAGLSASEEQIVPQQYANNPKIDAFIDEVAARDGFDPAKLHALFASANYSATAVRLVRPARSPSVKNWRAYQ
ARFLDAIRINAGVKFWRANQATLQRASAEFGVPPEVIVGIIGVETIYGRYMGNFPTLDALTTLAFDYPDTPNRDDRQATF
RKNLEDFLVWTRDSQLDPTSVLGSYTGAIGIPQFLPSSIRSYAVDYEGNGHIDLRASTADAIGSVANYLKQHGWEPGRPV
VWQIAPDAGSQGVAQAAADGKPEPHLKLSQLTQAGLVLDEPSVDPAAEAGTLVTVVDLPSPGRATEYKLGLKNFYVLTRY
NRSFFYALAVYQLGERVKQKMLATQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=362, Percent_Identity=38.9502762430939, Blast_Score=217, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45368; Mature: 45236

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRSSAVFHPLRFRSRLPLASLSLAAVVATLAACAQTPTEPQAAAPATPTAPVAPAAVP
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DAPASASAPAPASAPAAAPAAATAPQLVPAAGLSASEEQIVPQQYANNPKIDAFIDEVAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHCCCCCHHHHHHHHHH
RDGFDPAKLHALFASANYSATAVRLVRPARSPSVKNWRAYQARFLDAIRINAGVKFWRAN
HCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEECCCCEEEECC
QATLQRASAEFGVPPEVIVGIIGVETIYGRYMGNFPTLDALTTLAFDYPDTPNRDDRQAT
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHH
FRKNLEDFLVWTRDSQLDPTSVLGSYTGAIGIPQFLPSSIRSYAVDYEGNGHIDLRASTA
HHHCHHHHEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHEEEECCCCCEEEEEECCH
DAIGSVANYLKQHGWEPGRPVVWQIAPDAGSQGVAQAAADGKPEPHLKLSQLTQAGLVLD
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCEEEE
EPSVDPAAEAGTLVTVVDLPSPGRATEYKLGLKNFYVLTRYNRSFFYALAVYQLGERVKQ
CCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHH
KMLATQ
HHHCCC
>Mature Secondary Structure 
TFRSSAVFHPLRFRSRLPLASLSLAAVVATLAACAQTPTEPQAAAPATPTAPVAPAAVP
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DAPASASAPAPASAPAAAPAAATAPQLVPAAGLSASEEQIVPQQYANNPKIDAFIDEVAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHCCCCCHHHHHHHHHH
RDGFDPAKLHALFASANYSATAVRLVRPARSPSVKNWRAYQARFLDAIRINAGVKFWRAN
HCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEECCCCEEEECC
QATLQRASAEFGVPPEVIVGIIGVETIYGRYMGNFPTLDALTTLAFDYPDTPNRDDRQAT
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHH
FRKNLEDFLVWTRDSQLDPTSVLGSYTGAIGIPQFLPSSIRSYAVDYEGNGHIDLRASTA
HHHCHHHHEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHEEEECCCCCEEEEEECCH
DAIGSVANYLKQHGWEPGRPVVWQIAPDAGSQGVAQAAADGKPEPHLKLSQLTQAGLVLD
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCEEEE
EPSVDPAAEAGTLVTVVDLPSPGRATEYKLGLKNFYVLTRYNRSFFYALAVYQLGERVKQ
CCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHH
KMLATQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]