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The map label for this gene is gatA [H]

Identifier: 238026507

GI number: 238026507

Start: 920839

End: 922215

Strand: Reverse

Name: gatA [H]

Synonym: bglu_1g08510

Alternate gene names: 238026507

Gene position: 922215-920839 (Counterclockwise)

Preceding gene: 238026511

Following gene: 238026506

Centisome position: 23.61

GC content: 75.02

Gene sequence:

>1377_bases
ATGACCGCTTTCGCCCCCTTCCCCCCGCTCGCCACGCTGGCCGCCGATCTCGCGGCCGGCCGCACGACCAGCCGCGCGCT
CGTCGACGCCGCGCTCGACCGCATCGCGGCGCCCGACGGCCAGGGTGCGGTCGCGTTCACCGCCGTCGACGCCGACGCGG
CCCGCGCGACGGCCGACGCGCACGACCGCCTGCGCGCGGCCGGCACGGTGCTGTCGCCGCTGGCCGGCATCCCGGTGTCG
GTCAAGGACCTGTTCGACATCGCCGGCCAGGTCACGCGGGCCGGCTCGCGCGCGCTCGCCGACGCGCCGCCGGCGGCGGC
CGACGCGCCGGCGGTGGCACGGCTGCGCCGGGCCGGCGCGGTGCTGGTCGGGCGCACCAACATGAGCGAATTCGCGTTCT
CCGGGCTCGGCCTGAACCCGCACTACGGCACGCCGCGCTCGCCTTACCGGCGCGCGGTGGCGGGCGACGCGCGCGTGGCC
GGCGGCTCATCGTCGGGTGCCGCCGTTTCGGTGGCCGACGGCATGGCGGCCGCCGCACTCGGCACCGACACCGGCGGCTC
GCTGCGGATTCCGGCCGCGCTGTGCGGGCTGACCGGCTTCAAGCCGACTGCGAGCCGGGTGCCGAAGGACGGCGGCGTAC
CGCTATCGTCGACGCTCGACTCGTTCGGCCCGATCGGCGTGTCGGTGGCCTGCTGCGCGCTGGTGGACCGGATTCTGGCC
GGGCTCGCGCCGCGCGTGCCGGCCATGCGGCCGCTCGAAGGCGTGCGGCTCGGCGTGCTCGATCACTATGTGACGGACGG
CATGGACGCCGAGGTGACGGCGGCGTTCAGCGCCGCGCTCGAACGTCTGGCGGCGGCCGGCGCAATCCTGTCCGACCTGC
GCTTCGCCCCGCTCGAGCGGCTCGACGATATCAACCGCTTCGGCTTCTCGCCGATCGAGGCCTACGCCTGGCACCGGCCC
TTGCTCGAGCGCCGCCGCGACACCTACGATCCGCGCGTGCTGGCGCGCATCATGAAGGGCCAGCCCGCCACGGCTGCCGA
TTATCTCGATCTGCTCGCCGCCCGCGCGGCCATGCTCGACGAGGCCGAGCGCACGCTCTGGCCGAGCGTGGACGCGGTGC
TCGCGCCGACCGTGCCGGTGGTGCCGCCGCGCCTGGCCGAACTCGAACGCGACGACGACGCGTTCGCGCGCACCAACGCG
CTGATCCTGCGCAATCCGAGCGCGTTCAACTTCCTCGACGCCTGCGCGCTGTCGGTACCATGCCATCAGCGCGACGGCGC
ACCGGTCGGCCTGATGATCGCGGCCGCGCCCCATCGCGACGACACGCTGCTCGCCATCGGCCAGTCGATCGAGGCGGTGC
TCGCGACGAGCCGCTGA

Upstream 100 bases:

>100_bases
GATCCCCCTACCATCGACAGCCTGCGAGCCGGCACGCCGCGCAAGCCACGCGACGCCAGCCGACGCCCCACCCTTCGCCT
GCTCTCGCCCCGACGCCGCC

Downstream 100 bases:

>100_bases
CCGGCCCGCGGCGCGCGGCAGTTGCCGGCCATCCGCCGCACGGCGCGGCGCCTGCCACCCGGCGAGCGCCGGCCCGGTTG
GCCGACGTGCGCGAAGTCGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTAFAPFPPLATLAADLAAGRTTSRALVDAALDRIAAPDGQGAVAFTAVDADAARATADAHDRLRAAGTVLSPLAGIPVS
VKDLFDIAGQVTRAGSRALADAPPAAADAPAVARLRRAGAVLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRAVAGDARVA
GGSSSGAAVSVADGMAAAALGTDTGGSLRIPAALCGLTGFKPTASRVPKDGGVPLSSTLDSFGPIGVSVACCALVDRILA
GLAPRVPAMRPLEGVRLGVLDHYVTDGMDAEVTAAFSAALERLAAAGAILSDLRFAPLERLDDINRFGFSPIEAYAWHRP
LLERRRDTYDPRVLARIMKGQPATAADYLDLLAARAAMLDEAERTLWPSVDAVLAPTVPVVPPRLAELERDDDAFARTNA
LILRNPSAFNFLDACALSVPCHQRDGAPVGLMIAAAPHRDDTLLAIGQSIEAVLATSR

Sequences:

>Translated_458_residues
MTAFAPFPPLATLAADLAAGRTTSRALVDAALDRIAAPDGQGAVAFTAVDADAARATADAHDRLRAAGTVLSPLAGIPVS
VKDLFDIAGQVTRAGSRALADAPPAAADAPAVARLRRAGAVLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRAVAGDARVA
GGSSSGAAVSVADGMAAAALGTDTGGSLRIPAALCGLTGFKPTASRVPKDGGVPLSSTLDSFGPIGVSVACCALVDRILA
GLAPRVPAMRPLEGVRLGVLDHYVTDGMDAEVTAAFSAALERLAAAGAILSDLRFAPLERLDDINRFGFSPIEAYAWHRP
LLERRRDTYDPRVLARIMKGQPATAADYLDLLAARAAMLDEAERTLWPSVDAVLAPTVPVVPPRLAELERDDDAFARTNA
LILRNPSAFNFLDACALSVPCHQRDGAPVGLMIAAAPHRDDTLLAIGQSIEAVLATSR
>Mature_457_residues
TAFAPFPPLATLAADLAAGRTTSRALVDAALDRIAAPDGQGAVAFTAVDADAARATADAHDRLRAAGTVLSPLAGIPVSV
KDLFDIAGQVTRAGSRALADAPPAAADAPAVARLRRAGAVLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRAVAGDARVAG
GSSSGAAVSVADGMAAAALGTDTGGSLRIPAALCGLTGFKPTASRVPKDGGVPLSSTLDSFGPIGVSVACCALVDRILAG
LAPRVPAMRPLEGVRLGVLDHYVTDGMDAEVTAAFSAALERLAAAGAILSDLRFAPLERLDDINRFGFSPIEAYAWHRPL
LERRRDTYDPRVLARIMKGQPATAADYLDLLAARAAMLDEAERTLWPSVDAVLAPTVPVVPPRLAELERDDDAFARTNAL
ILRNPSAFNFLDACALSVPCHQRDGAPVGLMIAAAPHRDDTLLAIGQSIEAVLATSR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=24.748490945674, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI195972892, Length=144, Percent_Identity=36.1111111111111, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI166795287, Length=192, Percent_Identity=32.8125, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=25.0569476082005, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=205, Percent_Identity=30.2439024390244, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=170, Percent_Identity=36.4705882352941, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=25.8585858585859, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=135, Percent_Identity=37.7777777777778, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=154, Percent_Identity=38.3116883116883, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=154, Percent_Identity=38.3116883116883, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=387, Percent_Identity=30.4909560723514, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=25.462962962963, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648113, Length=303, Percent_Identity=32.6732673267327, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648435, Length=223, Percent_Identity=29.5964125560538, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648437, Length=223, Percent_Identity=29.5964125560538, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI45550774, Length=223, Percent_Identity=29.5964125560538, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648441, Length=223, Percent_Identity=29.5964125560538, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648439, Length=223, Percent_Identity=29.5964125560538, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21356731, Length=137, Percent_Identity=35.7664233576642, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652985, Length=420, Percent_Identity=24.2857142857143, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922090, Length=420, Percent_Identity=24.2857142857143, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652981, Length=420, Percent_Identity=24.2857142857143, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652983, Length=420, Percent_Identity=24.2857142857143, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47282; Mature: 47151

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFAPFPPLATLAADLAAGRTTSRALVDAALDRIAAPDGQGAVAFTAVDADAARATADA
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHH
HDRLRAAGTVLSPLAGIPVSVKDLFDIAGQVTRAGSRALADAPPAAADAPAVARLRRAGA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHCCE
VLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRAVAGDARVAGGSSSGAAVSVADGMAAAAL
EEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCCEEEECCCHHHHHC
GTDTGGSLRIPAALCGLTGFKPTASRVPKDGGVPLSSTLDSFGPIGVSVACCALVDRILA
CCCCCCCEECHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
GLAPRVPAMRPLEGVRLGVLDHYVTDGMDAEVTAAFSAALERLAAAGAILSDLRFAPLER
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDINRFGFSPIEAYAWHRPLLERRRDTYDPRVLARIMKGQPATAADYLDLLAARAAMLD
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EAERTLWPSVDAVLAPTVPVVPPRLAELERDDDAFARTNALILRNPSAFNFLDACALSVP
HHHHHHCCCCHHHHCCCCCCCCCHHHHCCCCCHHHHHCCEEEEECCCHHHHHHHHHHCCC
CHQRDGAPVGLMIAAAPHRDDTLLAIGQSIEAVLATSR
CCCCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
TAFAPFPPLATLAADLAAGRTTSRALVDAALDRIAAPDGQGAVAFTAVDADAARATADA
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHH
HDRLRAAGTVLSPLAGIPVSVKDLFDIAGQVTRAGSRALADAPPAAADAPAVARLRRAGA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHCCE
VLVGRTNMSEFAFSGLGLNPHYGTPRSPYRRAVAGDARVAGGSSSGAAVSVADGMAAAAL
EEEECCCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCCEEEECCCHHHHHC
GTDTGGSLRIPAALCGLTGFKPTASRVPKDGGVPLSSTLDSFGPIGVSVACCALVDRILA
CCCCCCCEECHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
GLAPRVPAMRPLEGVRLGVLDHYVTDGMDAEVTAAFSAALERLAAAGAILSDLRFAPLER
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDDINRFGFSPIEAYAWHRPLLERRRDTYDPRVLARIMKGQPATAADYLDLLAARAAMLD
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EAERTLWPSVDAVLAPTVPVVPPRLAELERDDDAFARTNALILRNPSAFNFLDACALSVP
HHHHHHCCCCHHHHCCCCCCCCCHHHHCCCCCHHHHHCCEEEEECCCHHHHHHHHHHCCC
CHQRDGAPVGLMIAAAPHRDDTLLAIGQSIEAVLATSR
CCCCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA