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The map label for this gene is spoT [H]

Identifier: 238026488

GI number: 238026488

Start: 896614

End: 898989

Strand: Direct

Name: spoT [H]

Synonym: bglu_1g08320

Alternate gene names: 238026488

Gene position: 896614-898989 (Clockwise)

Preceding gene: 238026487

Following gene: 238026489

Centisome position: 22.95

GC content: 65.78

Gene sequence:

>2376_bases
ATGAGCAACTCACCATCGTCCGCCTCCGCGGAAGCCGGCCCGGCCGAAAGCACGGAAGCGACGGCCACCTCGCCGGCGCG
GCAATACATCGACGCGGTCCTCGAACAGTCGTTTCGCCACCTGTTCGGGCCGACCGCCACGCCGGAGCAGCCTCGCAAGC
ACGGCGTCGTTTCGATCGCGAAGCTGACGGCCGCGCTTGCCGAGTATCTGCCCCCGGAAGAAATCAAAGAGGTGAAGGCG
GCGTTTCACTTCAGTGACGAAGCCCACCTCGGTCAATATCGCCAGAGCGGCGAACCCTACATCACCCATCCCGTCGCCGT
CGCCGAAATCTGCGCCGGCTGGAAGCTCGACGCCCAGGCCGTCATGGCCGCGTTGCTGCACGACGTGATGGAGGACCAGG
GCGTCATGAAGAGCGAACTCGCTGAGCGCTTCGGCCCGAAGGTGGCCGAGCTCGTTGACGGGCTCTCCAAGCTCGACAAG
ATGGAGTTTCGCAGCCGCGAGGAAGCGCAGGCGGAAAACTTCCGCAAGATGCTGCTCGCGATGGCGCGCGACGTGCGCGT
GATCCTCGTGAAGCTGGCCGACCGCCTGCACAACATGCGCACGCTCGGCGCGGTGCCGATGGAGAAGCGCCGGCGCGTGG
CGCGCGAGACGCTCGACATCTACGCGCCGATCGCGCATCGGCTCGGCCTGAACAACACCTATCGCGAGCTGCAGGAGATG
AGCTTCGCGAACTTCAACCCGCATCGCTACGCGACGCTCGAGAAGGCCGTGAAGGCCGCGCGCGGCAATCGCCGCGAGGT
GGTCGGCAAGATCCTCGAGGCCGCGCATCGCACGGTGGTGGACGCCAAGCTCGATGCCGAGATCACCGGCCGCGAGAAGA
CCATCTACAGCATCTACCGCAAGATGCGCGACAAGCAGCTGTCGTTCTCGCAGGTGCTCGACGTATACGGCTTTCGCGTG
GTGGTCGAGCATCCGCTCGAGTGCTATACCTGCATCGGTGCGCTGCATGCGCTCTACAAGCCGGTGCCGGGCAAGTTCAA
GGATTACATCGCGATCCCGAAGGTGAACGGCTACCAGTCGCTGCACACCACGGTGGTCGGCCCGTTCGGCGCGCCGATCG
AGTTCCAGGTCCGTACCCGCAAGATGCACGAAATCGCCGAGGCAGGCGTGGCCGCGCACTGGCTCTACAAGAATGGCGGT
GCCGACCTCAACGACGTGCAGAAGCGCGCCCACCAGTGGCTCAAGTCGCTGCTCGATATTCAGAGCGAGGCGGGCGACTC
AAGCGAGTTCCTCGAACACGTCAAGATCGACCTGTTCCCCGACGCCGTCTACGTGTTCACGCCGAAGTCGAAGATCATGG
CGCTGCCGCGCGGCGCGACGGCGCTCGACTTCGCCTACGCCGTGCACAGCGATCTCGGCAACCAGTGCGTGGCGGTCAAG
ATCAACAACGAACTGCTGCCGCTGCGCACCGAACTCAAGAGCGGCGACATCGTCGAGGTGATCACGGCGCCGTACTCGAA
GCCGAACCCGGTCTGGCTCGGCTTCGTGCGTACCGGCAAGGCGCGTTCGGCGATCCGCCACTACCTGAAGACGATGCGTC
TGAACGAATCGGTGCAGCTCGGCGAACGGCTCGTCGACCAGAGCCTCAAGGGTTACGGTCTCGCGCTGGCCGACGTGCAG
CCCGATGTATGGGACAAGCTCGTGCAGTGGACCGGCAACAAGAGCCGCCAGGAAATCTTCGCGGACATCGGCCTCGGCCG
GCGCGTGCCCGCGGTGATGGCCAAGCGCATCGAGGTGCTGATGAGCGGGCGCGACGCCGACGACGATCTGCCGCGCGCCG
ACCGTCCGGCGCACAACGCGCCGCCGGTCGCGATCACCGGCACCGAGGGGATGTCGGTGCAGCTCTCGGCGTGCTGCCGG
CCGATTCCCGGCGACAGCATCATGGGCTACATCGGCATCGGTCTGGGCATGGCGATCCATACGACGGATTGCCGCGTGGC
GCAGCGCATTCACCGGCGCGACCCGGGCCGCTGGATCGACGTTGCCTGGGCGCCGCAGCCGGGCCGCCTGTTCGACGTGG
CGGTGAAGGCGCTGGTGAAGAATACCAAGGGTATTTTCGCGCGCGTCGCGGCCGACATCACCTCGGCCGACGCGAATATC
GTCCACATTGCGATGGACGAGGATCTGTCGCACGAATCGACGGTGCTGCGCTTCGTGATCCAGGTGAGCGACCGCGTCCA
CCTCGCGAACGTGATGCGGCGCGTGCGAACCAACCCGGACGTGATGCGGATCATGCGCGAGCGGTCGAGCGACGACACGA
CCCATACGCGCCACGACGGCGGGATGCGCATCGACCGGGAACGGCACGACTACTGA

Upstream 100 bases:

>100_bases
CGGCATCGAGATGCTGAAGAAAGTGCCGGTTTAAGGCACACTCGGTCACTCGAGTCATGTAGTCCCGTGCAACCCGACGT
GCCAACCTGAGGAGGCGAAT

Downstream 100 bases:

>100_bases
CAGGCCGGCGGCGCCGGCTGCCGGGCCAGGAAAAATCCGACCCGAAAAACAAAAGGGCCTTCCCGGAGGAAGGCCCTTCA
AAATATGGCGCGGCTGGCAG

Product: Guanosine polyphosphate pyrophosphohydrolases/synthetase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 791; Mature: 790

Protein sequence:

>791_residues
MSNSPSSASAEAGPAESTEATATSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPEEIKEVKA
AFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVMKSELAERFGPKVAELVDGLSKLDK
MEFRSREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQEM
SFANFNPHRYATLEKAVKAARGNRREVVGKILEAAHRTVVDAKLDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRV
VVEHPLECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTVVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGG
ADLNDVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYAVHSDLGNQCVAVK
INNELLPLRTELKSGDIVEVITAPYSKPNPVWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVQ
PDVWDKLVQWTGNKSRQEIFADIGLGRRVPAVMAKRIEVLMSGRDADDDLPRADRPAHNAPPVAITGTEGMSVQLSACCR
PIPGDSIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANI
VHIAMDEDLSHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSSDDTTHTRHDGGMRIDRERHDY

Sequences:

>Translated_791_residues
MSNSPSSASAEAGPAESTEATATSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPEEIKEVKA
AFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVMKSELAERFGPKVAELVDGLSKLDK
MEFRSREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQEM
SFANFNPHRYATLEKAVKAARGNRREVVGKILEAAHRTVVDAKLDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRV
VVEHPLECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTVVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGG
ADLNDVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYAVHSDLGNQCVAVK
INNELLPLRTELKSGDIVEVITAPYSKPNPVWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVQ
PDVWDKLVQWTGNKSRQEIFADIGLGRRVPAVMAKRIEVLMSGRDADDDLPRADRPAHNAPPVAITGTEGMSVQLSACCR
PIPGDSIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANI
VHIAMDEDLSHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSSDDTTHTRHDGGMRIDRERHDY
>Mature_790_residues
SNSPSSASAEAGPAESTEATATSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPEEIKEVKAA
FHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVMKSELAERFGPKVAELVDGLSKLDKM
EFRSREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQEMS
FANFNPHRYATLEKAVKAARGNRREVVGKILEAAHRTVVDAKLDAEITGREKTIYSIYRKMRDKQLSFSQVLDVYGFRVV
VEHPLECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTVVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGA
DLNDVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYAVHSDLGNQCVAVKI
NNELLPLRTELKSGDIVEVITAPYSKPNPVWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVQP
DVWDKLVQWTGNKSRQEIFADIGLGRRVPAVMAKRIEVLMSGRDADDDLPRADRPAHNAPPVAITGTEGMSVQLSACCRP
IPGDSIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIV
HIAMDEDLSHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSSDDTTHTRHDGGMRIDRERHDY

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=709, Percent_Identity=45.5571227080395, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=692, Percent_Identity=31.0693641618497, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 88237; Mature: 88106

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSPSSASAEAGPAESTEATATSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHH
KLTAALAEYLPPEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQA
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHH
VMAALLHDVMEDQGVMKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLA
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQEM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SFANFNPHRYATLEKAVKAARGNRREVVGKILEAAHRTVVDAKLDAEITGREKTIYSIYR
HCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHH
KMRDKQLSFSQVLDVYGFRVVVEHPLECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQS
HHHHHCCCHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHH
LHTTVVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDI
HHHHEECCCCCCEEEEHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHH
QSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYAVHSDLGNQCVAVK
HHCCCCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEE
INNELLPLRTELKSGDIVEVITAPYSKPNPVWLGFVRTGKARSAIRHYLKTMRLNESVQL
ECCCEECHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCHHHH
GERLVDQSLKGYGLALADVQPDVWDKLVQWTGNKSRQEIFADIGLGRRVPAVMAKRIEVL
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
MSGRDADDDLPRADRPAHNAPPVAITGTEGMSVQLSACCRPIPGDSIMGYIGIGLGMAIH
HCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHCCEEE
TTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANI
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VHIAMDEDLSHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSSDDTTHTRHDG
EEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
GMRIDRERHDY
CCEECHHCCCC
>Mature Secondary Structure 
SNSPSSASAEAGPAESTEATATSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHH
KLTAALAEYLPPEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQA
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHH
VMAALLHDVMEDQGVMKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLA
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQEM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SFANFNPHRYATLEKAVKAARGNRREVVGKILEAAHRTVVDAKLDAEITGREKTIYSIYR
HCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHH
KMRDKQLSFSQVLDVYGFRVVVEHPLECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQS
HHHHHCCCHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHH
LHTTVVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDI
HHHHEECCCCCCEEEEHHHHHHHHHHHCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHH
QSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYAVHSDLGNQCVAVK
HHCCCCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEE
INNELLPLRTELKSGDIVEVITAPYSKPNPVWLGFVRTGKARSAIRHYLKTMRLNESVQL
ECCCEECHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCHHHH
GERLVDQSLKGYGLALADVQPDVWDKLVQWTGNKSRQEIFADIGLGRRVPAVMAKRIEVL
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
MSGRDADDDLPRADRPAHNAPPVAITGTEGMSVQLSACCRPIPGDSIMGYIGIGLGMAIH
HCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHCCEEE
TTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANI
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VHIAMDEDLSHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSSDDTTHTRHDG
EEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
GMRIDRERHDY
CCEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]