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The map label for this gene is pcnB [H]

Identifier: 238026306

GI number: 238026306

Start: 681709

End: 683286

Strand: Reverse

Name: pcnB [H]

Synonym: bglu_1g06400

Alternate gene names: 238026306

Gene position: 683286-681709 (Counterclockwise)

Preceding gene: 238026307

Following gene: 238026305

Centisome position: 17.49

GC content: 71.55

Gene sequence:

>1578_bases
GTGATCAAGAAACTCATCCGCAAGCTGCTCGGGCAGGACGATTCCGCCGCCCGCGACAACGCCGGCGCGGCCGATGCCGC
CCCGCCCTCCACCGGCCCCGCGGCCGCCTCGCCGGCCGCCGTGGCAGCCGCCAAGAAACAGGCCGCGCGCGACGCCGCGA
AGGTCGCCGCGAGCCGCACCGAGCCGACCATCGTGCCGGCCGCCGTGCATGGCATCGACCCGTCGCTGATCTCGCGCAAC
GCCGTGCGCGTGACCGATACGCTGCAGCAGGCCGGCTTCCGCGCGTTCATCGTCGGCGGCGCGGTACGCGACCTGCTGCT
CGGCATCGCCCCGAAGGATTTCGACGTCGCCACCGACGCGACGCCCACCGAGGTGCAGCGCCTGTTCCGCCGCGCCCGGC
TGATCGGCCGGCGTTTCCAGATCGTCCACGTGCAGTTCGGCCAGGAGCTGATCGAGGTCTCGACCTTCCGCGCGCTGGTG
GACGCGCCGGCCGACGCGCCCGCGTCGGCGCCCGCCGACGCCGCCCCGCCGCGGCGCATGAAGCGCGACGAGCTGGACCG
CCGCACCCATGCGGTGGACGCGAGCGGGCGCGTGCTGCGCGACAACGTCTGGGGCGAGCAGCACGAGGACGCGGCGCGCC
GCGACTTCACGATCAACGCGATGTACTACGACCCGGCGACCCAGACCGTGCTCGACTACCACGACGGCATGGCCGACGTG
CGCGCACGATTGTTGCGCATGATCGGCGACCCGGCCACGCGCTACCGCGAGGATCCGGTGCGGATGCTGCGCGTGGTGCG
CTTCGCGGCCAAGCTCGGCTTCGACATCGAGCCGCACACGCGCGAGCCGATCCAGCCGCTCGCCGATCTGATCAACAACG
TGCCCGCCGCGCGCCTGTTCGACGAGATGCTGAAGCTGCTGCTCTCGGGCCATGCGCTCGCCTGCCTCAGGCAGTTGCGC
AGCGAGGGCCTGCACCATGGGCTGCTGCCGCTGCTCGACGTGGTGCTGGAGCAGCCGCAGGGCGAGAAGTTCATCACGCT
CGCGCTCAACAACACCGACGCGCGCGTGCGCGCCGGCAAGTCCGTGTCGCCGGGCTTTTTGTTCGCGACGCTGCTCTGGC
ACGACATGCGCCAGCGCTGGAACCAGAACGTCGCGAACGGCGAGTTCCCGGTGCCGGCGCTGCAGCGCGCGATGGACGAC
GTGCTCGAGATGCAGACCGAGAAGCTCGCGATCCACAAGCGCTACTCGGCCGACATGCGCGAGATCTGGGGGCTGCAGCT
GCGCCTCGAGAAACGCTCGGGCCGCAGCGCCATGAGGCTGCTGGAACACCAAAGGTTTAGAGCGGGGTATGATTTCCTCC
TCCTGCGCTGCGAGTCGGGCGAGCTGGACCCGGCCGTGGCGCAATGGTGGACGGATTTCATCGACGGCGATGCCGCGGCT
CGCGAGGCCTTGCTCACGAACGGCGCCCCGCGCGATCCGGCGGCACCGCGCAAGCGCCGCCGCCGCGGCGGCGCGCGCAA
CCGCAGGCCGGCCGACGGCACCGTGCAACAGGCCGACGCCCAGGACGCGCAGGACTGA

Upstream 100 bases:

>100_bases
TCGAGAAAGTGACCGATCCGGTCGCGACCAACCCCGACGACACGCTGCGTGCGCATGCCCGCGAACACGGCTGGCGCGTC
CTCGATCTCTTCCAGCCCTC

Downstream 100 bases:

>100_bases
GCCGTCGAACGACGTAGGATGTGGATGCCATGACGGTTGCTTATCTCGGGCTGGGCGCGAATCTCGGCGATGCGCGCCAG
GCGTTGAAGGACGCGGTGGT

Product: putative polynucleotide adenylyltransferase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MIKKLIRKLLGQDDSAARDNAGAADAAPPSTGPAAASPAAVAAAKKQAARDAAKVAASRTEPTIVPAAVHGIDPSLISRN
AVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALV
DAPADAPASAPADAAPPRRMKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPATQTVLDYHDGMADV
RARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFDIEPHTREPIQPLADLINNVPAARLFDEMLKLLLSGHALACLRQLR
SEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKSVSPGFLFATLLWHDMRQRWNQNVANGEFPVPALQRAMDD
VLEMQTEKLAIHKRYSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDPAVAQWWTDFIDGDAAA
REALLTNGAPRDPAAPRKRRRRGGARNRRPADGTVQQADAQDAQD

Sequences:

>Translated_525_residues
MIKKLIRKLLGQDDSAARDNAGAADAAPPSTGPAAASPAAVAAAKKQAARDAAKVAASRTEPTIVPAAVHGIDPSLISRN
AVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALV
DAPADAPASAPADAAPPRRMKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPATQTVLDYHDGMADV
RARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFDIEPHTREPIQPLADLINNVPAARLFDEMLKLLLSGHALACLRQLR
SEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKSVSPGFLFATLLWHDMRQRWNQNVANGEFPVPALQRAMDD
VLEMQTEKLAIHKRYSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDPAVAQWWTDFIDGDAAA
REALLTNGAPRDPAAPRKRRRRGGARNRRPADGTVQQADAQDAQD
>Mature_525_residues
MIKKLIRKLLGQDDSAARDNAGAADAAPPSTGPAAASPAAVAAAKKQAARDAAKVAASRTEPTIVPAAVHGIDPSLISRN
AVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALV
DAPADAPASAPADAAPPRRMKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPATQTVLDYHDGMADV
RARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFDIEPHTREPIQPLADLINNVPAARLFDEMLKLLLSGHALACLRQLR
SEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKSVSPGFLFATLLWHDMRQRWNQNVANGEFPVPALQRAMDD
VLEMQTEKLAIHKRYSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDPAVAQWWTDFIDGDAAA
REALLTNGAPRDPAAPRKRRRRGGARNRRPADGTVQQADAQDAQD

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=429, Percent_Identity=44.7552447552448, Blast_Score=340, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 58122; Mature: 58122

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKLIRKLLGQDDSAARDNAGAADAAPPSTGPAAASPAAVAAAKKQAARDAAKVAASRT
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EPTIVPAAVHGIDPSLISRNAVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDA
CCCEEHHHHHCCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCC
TPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPADAPASAPADAAPPRRM
CHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPATQTVLDYHDGMADV
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHCCEEEEEEEECCCHHHHHHHHCCHHHH
RARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFDIEPHTREPIQPLADLINNVPAARLF
HHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHH
DEMLKLLLSGHALACLRQLRSEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGK
HHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCC
SVSPGFLFATLLWHDMRQRWNQNVANGEFPVPALQRAMDDVLEMQTEKLAIHKRYSADMR
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDPAVAQWWTDFIDGDAAA
HHCCHHEEEEHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHH
REALLTNGAPRDPAAPRKRRRRGGARNRRPADGTVQQADAQDAQD
HHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MIKKLIRKLLGQDDSAARDNAGAADAAPPSTGPAAASPAAVAAAKKQAARDAAKVAASRT
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EPTIVPAAVHGIDPSLISRNAVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDA
CCCEEHHHHHCCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCC
TPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPADAPASAPADAAPPRRM
CHHHHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPATQTVLDYHDGMADV
HHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHCCEEEEEEEECCCHHHHHHHHCCHHHH
RARLLRMIGDPATRYREDPVRMLRVVRFAAKLGFDIEPHTREPIQPLADLINNVPAARLF
HHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHH
DEMLKLLLSGHALACLRQLRSEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGK
HHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHCCCC
SVSPGFLFATLLWHDMRQRWNQNVANGEFPVPALQRAMDDVLEMQTEKLAIHKRYSADMR
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDPAVAQWWTDFIDGDAAA
HHCCHHEEEEHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHH
REALLTNGAPRDPAAPRKRRRRGGARNRRPADGTVQQADAQDAQD
HHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]