Click here to switch to the map view.

The map label for this gene is grpE [H]

Identifier: 238026299

GI number: 238026299

Start: 673498

End: 674061

Strand: Direct

Name: grpE [H]

Synonym: bglu_1g06330

Alternate gene names: 238026299

Gene position: 673498-674061 (Clockwise)

Preceding gene: 238026297

Following gene: 238026300

Centisome position: 17.24

GC content: 68.62

Gene sequence:

>564_bases
ATGGAAAACACGCAAGAGAATCCGGCTGGTCAACCGGCCGACGAGAGCGGCCGCGACGCGCAGGCTGCGCAACCCCAGGC
CGCCGAAGCGGCGCCGGCGCAGCCGGCAGGCGAGGCCGAGGCCGCGCTGGCCGAGGCACAGGCCAAGATCGCCGAGCTGC
AGGAAAGCTTCCTGCGGGCCAAGGCCGAAACCGAGAACGTGCGCCGCCGCGGCCAGGAAGACGTTGCCAAGGCCCACAAG
TTCGCGATCGAGAGCTTCGCCGAGCATCTGCTGCCGGTGATCGACAGCCTGGAAGCCGCGGTCGGCGACAAGTCCGAGGA
CATCGCGAAGGTCCGCGAGGGCGTCGAGCTGACGCTGCGCCAGTTGCAGAGCGCGCTCGAGAAGGGCCGCGTCAGCGTGA
TCGATCCGGCCGGCGCGAAGTTCGATCCGCACCAGCACCAGGCCATCTCGATGGTGCCGGCCGAGCAGGAGCCGAATACC
GTCGTCACCGTGCTGCAAAAGGGCTATATGATCGCCGACCGCGTGCTGCGCCCCGCGCTCGTGACGGTGTCGCAGCCGAA
GTAA

Upstream 100 bases:

>100_bases
GGTGTCTTCGGCCCCATCTGCGCCAACGTTGTGCGGCGGTGCGTTGCCCGTGCGAGGCGCCGCCGGTCTGGCTTTCATCA
CTCAATTCGATTTCAGCGAC

Downstream 100 bases:

>100_bases
GCCAGCACGCCGCCGTGCGGCGGCCGGCATGGCTTGAGCGCCGCCCGGCCGGCGTCCGGGGCGCCGCGCCGCGCGGGCCT
CGCCGGCGGCGGTGGCGCGA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MENTQENPAGQPADESGRDAQAAQPQAAEAAPAQPAGEAEAALAEAQAKIAELQESFLRAKAETENVRRRGQEDVAKAHK
FAIESFAEHLLPVIDSLEAAVGDKSEDIAKVREGVELTLRQLQSALEKGRVSVIDPAGAKFDPHQHQAISMVPAEQEPNT
VVTVLQKGYMIADRVLRPALVTVSQPK

Sequences:

>Translated_187_residues
MENTQENPAGQPADESGRDAQAAQPQAAEAAPAQPAGEAEAALAEAQAKIAELQESFLRAKAETENVRRRGQEDVAKAHK
FAIESFAEHLLPVIDSLEAAVGDKSEDIAKVREGVELTLRQLQSALEKGRVSVIDPAGAKFDPHQHQAISMVPAEQEPNT
VVTVLQKGYMIADRVLRPALVTVSQPK
>Mature_187_residues
MENTQENPAGQPADESGRDAQAAQPQAAEAAPAQPAGEAEAALAEAQAKIAELQESFLRAKAETENVRRRGQEDVAKAHK
FAIESFAEHLLPVIDSLEAAVGDKSEDIAKVREGVELTLRQLQSALEKGRVSVIDPAGAKFDPHQHQAISMVPAEQEPNT
VVTVLQKGYMIADRVLRPALVTVSQPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=190, Percent_Identity=35.2631578947368, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI40255109, Length=146, Percent_Identity=36.3013698630137, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1788967, Length=195, Percent_Identity=42.0512820512821, Blast_Score=125, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17552458, Length=171, Percent_Identity=34.5029239766082, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=152, Percent_Identity=32.8947368421053, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653432, Length=173, Percent_Identity=35.2601156069364, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20046; Mature: 20046

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENTQENPAGQPADESGRDAQAAQPQAAEAAPAQPAGEAEAALAEAQAKIAELQESFLRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAETENVRRRGQEDVAKAHKFAIESFAEHLLPVIDSLEAAVGDKSEDIAKVREGVELTLR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QLQSALEKGRVSVIDPAGAKFDPHQHQAISMVPAEQEPNTVVTVLQKGYMIADRVLRPAL
HHHHHHHHCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHH
VTVSQPK
HCCCCCC
>Mature Secondary Structure
MENTQENPAGQPADESGRDAQAAQPQAAEAAPAQPAGEAEAALAEAQAKIAELQESFLRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAETENVRRRGQEDVAKAHKFAIESFAEHLLPVIDSLEAAVGDKSEDIAKVREGVELTLR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QLQSALEKGRVSVIDPAGAKFDPHQHQAISMVPAEQEPNTVVTVLQKGYMIADRVLRPAL
HHHHHHHHCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHH
VTVSQPK
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA