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The map label for this gene is gdhA [H]

Identifier: 238026227

GI number: 238026227

Start: 589797

End: 591104

Strand: Direct

Name: gdhA [H]

Synonym: bglu_1g05580

Alternate gene names: 238026227

Gene position: 589797-591104 (Clockwise)

Preceding gene: 238026226

Following gene: 238026228

Centisome position: 15.1

GC content: 67.74

Gene sequence:

>1308_bases
ATGTCTTCGCAATCGCAGTCCTCGCCCGTCATCGCGCAGTCCATTCCGTCATATCTGAACGCGGAAGCGTTGGGCCCGTG
GGGCAACTACCTGCAGCAGGTCGACCGCGTCGCGCCGTATCTCGGCTCGCTGTCGCGCTGGCTCGAAACGCTGAAGCGCC
CGAAGCGCATCCTGGTGGTCGACGTGCCGATCGAGCTCGATAACGGCACCGTGGCGCACTTCGAGGGCTACCGCGTGCAG
CACAACGTCTCGCGCGGCCCGGGCAAGGGCGGCGTGCGCTACCACCAGGACGTCACGCTGTCCGAGGTGATGGCGCTGTC
GGCCTGGATGTCGGTCAAGAATGCGGCCGTCAACGTGCCGTACGGCGGCGCGAAGGGCGGCATCCGCGTCGATCCGCGCA
AGCTGTCGCGCGGCGAGCTCGAGCGCATGACGCGCCGCTACACGAGCGAGATCGGCATCATCATCGGGCCGAACACCGAT
ATCCCGGCACCGGACGTCAACACCAACGAACAGGTGATGGCGTGGATGATGGACACCTACTCGATGAACCAGGGCCAGAC
GGCCACCGGCGTGGTGACGGGCAAGCCGATCACGCTCGGCGGCTCGCTCGGCCGCCGCGAGGCGACCGGCCGCGGCGTGT
TCGTGGTGGGTTGCGAGGCGGCCCAGAAGATCGGCCTCGAGATCCGCGGCGCGCGCATCGCGGTGCAGGGCTTCGGCAAC
GTCGGCGGGATCGCGGGCAAGCTGTTCCAGGAGGCCGGCGCGACCGTCATCGCCGTGCAGGACCACACCGGCACGATCTA
CCAGCCGGCCGGCTTGGACACCGTGAAGCTGCTCGACCATGTGGCGCGCACGGGCGGCGTGGCGGGCTTCGAGGGCGCCG
AGTCGATGGCCAACGACGAGTTCTGGACCGTCGAGACCGACATCCTGATCCCGGCCGCGCTCGAGAACCAGATCACCGAG
AAGAACGCGGGCAAGATCCGCACGAAGATCGTCGTCGAGGGCGCGAACGGCCCGACCACCACGGCGGCCGACGACATCCT
CGCGGCCAATGGCGTGCTGGTGATTCCCGACGTGATCGCCAACGCGGGCGGCGTGACCGTCTCGTACTTCGAGTGGGTGC
AGGATTTCTCGAGCTTCTTCTGGACCGAGGACGAGATCAACCAGCGGCTCGAGCGCATCATGCGCGAGGCGTTCGCCGGC
GTCTGGTCGGTTGCGCAGGAGCACAATGTCACGGTGCGCACGGCGGCGTTCATCGTGGCCTGCAAGCGGATCCTGATGGC
GCGCGAGATGCGCGGCCTCTATCCCTGA

Upstream 100 bases:

>100_bases
CGATCATGCGCGAAACGCATAATCGAATAAACAAACGGCATTGGATTTCGAAAATCCGTTTTTCGACAATGTGCGTCATT
CGCTGACCGTTGGAGAATCC

Downstream 100 bases:

>100_bases
TCCCCCTGGACCGGCGGCTTGCCGCCGGTCGACGCGGCGGCGCGGCTCACCCCGCCGTGCCGCCACCCCCCCGCGGGCGA
TACCGATCCGGTATCGCCCG

Product: putative glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSSQSQSSPVIAQSIPSYLNAEALGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILVVDVPIELDNGTVAHFEGYRVQ
HNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERMTRRYTSEIGIIIGPNTD
IPAPDVNTNEQVMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAAQKIGLEIRGARIAVQGFGN
VGGIAGKLFQEAGATVIAVQDHTGTIYQPAGLDTVKLLDHVARTGGVAGFEGAESMANDEFWTVETDILIPAALENQITE
KNAGKIRTKIVVEGANGPTTTAADDILAANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLERIMREAFAG
VWSVAQEHNVTVRTAAFIVACKRILMAREMRGLYP

Sequences:

>Translated_435_residues
MSSQSQSSPVIAQSIPSYLNAEALGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILVVDVPIELDNGTVAHFEGYRVQ
HNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERMTRRYTSEIGIIIGPNTD
IPAPDVNTNEQVMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAAQKIGLEIRGARIAVQGFGN
VGGIAGKLFQEAGATVIAVQDHTGTIYQPAGLDTVKLLDHVARTGGVAGFEGAESMANDEFWTVETDILIPAALENQITE
KNAGKIRTKIVVEGANGPTTTAADDILAANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLERIMREAFAG
VWSVAQEHNVTVRTAAFIVACKRILMAREMRGLYP
>Mature_434_residues
SSQSQSSPVIAQSIPSYLNAEALGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILVVDVPIELDNGTVAHFEGYRVQH
NVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRKLSRGELERMTRRYTSEIGIIIGPNTDI
PAPDVNTNEQVMAWMMDTYSMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAAQKIGLEIRGARIAVQGFGNV
GGIAGKLFQEAGATVIAVQDHTGTIYQPAGLDTVKLLDHVARTGGVAGFEGAESMANDEFWTVETDILIPAALENQITEK
NAGKIRTKIVVEGANGPTTTAADDILAANGVLVIPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLERIMREAFAGV
WSVAQEHNVTVRTAAFIVACKRILMAREMRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=343, Percent_Identity=43.731778425656, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI4885281, Length=343, Percent_Identity=42.5655976676385, Blast_Score=273, Evalue=3e-73,
Organism=Escherichia coli, GI1788059, Length=360, Percent_Identity=34.7222222222222, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=425, Percent_Identity=38.1176470588235, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319256, Length=394, Percent_Identity=30.7106598984772, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=387, Percent_Identity=29.4573643410853, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45553475, Length=362, Percent_Identity=42.5414364640884, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI45549226, Length=368, Percent_Identity=42.3913043478261, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24649283, Length=331, Percent_Identity=43.202416918429, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24649460, Length=314, Percent_Identity=42.6751592356688, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46948; Mature: 46817

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQSQSSPVIAQSIPSYLNAEALGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILVV
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DVPIELDNGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVP
EEEEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEECC
YGGAKGGIRVDPRKLSRGELERMTRRYTSEIGIIIGPNTDIPAPDVNTNEQVMAWMMDTY
CCCCCCCEEECHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
SMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAAQKIGLEIRGARIAVQGFGN
CCCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEEECHHHHHCCEEEECCEEEEEECCC
VGGIAGKLFQEAGATVIAVQDHTGTIYQPAGLDTVKLLDHVARTGGVAGFEGAESMANDE
CCHHHHHHHHHCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCC
FWTVETDILIPAALENQITEKNAGKIRTKIVVEGANGPTTTAADDILAANGVLVIPDVIA
EEEEEECEEECHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEECHHHC
NAGGVTVSYFEWVQDFSSFFWTEDEINQRLERIMREAFAGVWSVAQEHNVTVRTAAFIVA
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
CKRILMAREMRGLYP
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSQSQSSPVIAQSIPSYLNAEALGPWGNYLQQVDRVAPYLGSLSRWLETLKRPKRILVV
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DVPIELDNGTVAHFEGYRVQHNVSRGPGKGGVRYHQDVTLSEVMALSAWMSVKNAAVNVP
EEEEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEECC
YGGAKGGIRVDPRKLSRGELERMTRRYTSEIGIIIGPNTDIPAPDVNTNEQVMAWMMDTY
CCCCCCCEEECHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHH
SMNQGQTATGVVTGKPITLGGSLGRREATGRGVFVVGCEAAQKIGLEIRGARIAVQGFGN
CCCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEEECHHHHHCCEEEECCEEEEEECCC
VGGIAGKLFQEAGATVIAVQDHTGTIYQPAGLDTVKLLDHVARTGGVAGFEGAESMANDE
CCHHHHHHHHHCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCC
FWTVETDILIPAALENQITEKNAGKIRTKIVVEGANGPTTTAADDILAANGVLVIPDVIA
EEEEEECEEECHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCEEEECHHHC
NAGGVTVSYFEWVQDFSSFFWTEDEINQRLERIMREAFAGVWSVAQEHNVTVRTAAFIVA
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
CKRILMAREMRGLYP
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]