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The map label for this gene is purB [H]

Identifier: 238026225

GI number: 238026225

Start: 587098

End: 588537

Strand: Reverse

Name: purB [H]

Synonym: bglu_1g05560

Alternate gene names: 238026225

Gene position: 588537-587098 (Counterclockwise)

Preceding gene: 238026232

Following gene: 238026220

Centisome position: 15.07

GC content: 67.01

Gene sequence:

>1440_bases
ATGGTAAAATTGCGGGTTGACCCTCTCCACGCCCGAACCGGCCGCCCCATCATGTCCGACACCCGCCCCGACACCCTTTT
CGCCCTCACCGCGCTCTCGCCGCTCGACGGCCGCTACGCGAGCAAGACCGAAGCGCTGCGCGAGTGGCTCTCCGAGGCCG
CGTTCATGCGCCACCGCGTGACGGTCGAGGCTCATTGGCTGATCGCGCTGTCGCGCGCCGGCTTCGCCGAAGTGCCGCGC
TTCTCGGCGGCCTCCGAGCAGTTCCTGCTGCAGCTGGTCGAGCGCTTCACCGCGCACGACGCGGCACGCATCAAGGACAT
CGAGCGCGTCACCAACCACGACGTGAAGGCGGTCGAATACTGGCTCAAGGAATCGGTGAAGGGCCAGCCGGAACTCGAGG
CCGCGAGCGAATTCATCCACTTCGCCTGCACCTCGGAAGACATCAACAACACCTCGCACGGCATGATGCTGGCCGGTGCG
CGCGACAAGGTGATCGTGCCGGCGCTGCGCAGCGTGCAGCAGCGGCTCGTCGCGCTCGCGCACGCGCTGGCCGACCAGCC
GATGCTGTCGCGCACGCACGGCCAGCCGGCCAGCCCGACCACGCTCGGCAAGGAAATCGCCAACGTCGCGGCGCGCCTCG
CGCGCGCGATCGAGCGCATCGAGAAGGTCGAGCTGCTGGGCAAGATGAACGGCGCGGTCGGCAACTTCAACGCGCACCTG
TCGGCGTATCCGGAATTCGACTGGGAGGCGTTCTCCAAGGACGTGGTCGAGAACCGCCTGAAGCTCGCCTTCAACCCGTA
CACCATCCAGATCGAGCCGCACGACTACATGGCCGAGCTGTTCGACGCGGTGGCGCGCGCCAACACGATCCTGCTCGACC
TCGACCGCGACGTCTGGGGCTACATCGCGCTCGGCTACTTCAAGCAGAAGACCAAGGCCGGCGAGATCGGCTCGTCGACC
ATGCCGCACAAGGTCAACCCGATCGATTTCGAGAACTCGGAAGGCAACCTCGGCCTGGCCAACGCCACGCTGCGCCATCT
CTCCGAGAAGCTGCCGGTGTCGCGCTGGCAGCGCGACCTGACCGACTCCACGGTGCTGCGCAACATCGGCGTGGCGTTCG
GCTACTCGCTGCTCGCCTACGAATCGCTCACGCGCGGTCTCGACAAGCTCGAGGTGAACCCCGCGCGCCTGAACGAGGAC
CTCGACAACTGCTGGGAAGTGCTGGCCGAGCCGGTGCAGACCGTGATGCGCCGCTACGGCATCGAGAACCCCTACGAGCA
GCTGAAGGAACTGACGCGCGGCAAGGGCATCACGCGCGAGGCGCTGCAGCAGTTCGTGGGCGGCCTGGCGATCCCGCAGG
ACGCGAAGGACCTGCTGCTGGCGATGACGCCGGCCTCGTACATCGGCAAGGCGGTCGAGCTCGCGAAGCGGATCGCGTAA

Upstream 100 bases:

>100_bases
CCCGCATTGTAGCCGGGCGGCGGCGGCGCAGGCGAAGGCGCCCGCGCGGGCCGCCCCCCGGCGCCCTGCCCGCACCGCGT
GCCGGAGCCGGCCGCCGCCG

Downstream 100 bases:

>100_bases
CGCGCGAGGCATCACGGCGTGCCGCCTCGCGGCGGCACGCAAAAACCAAGGCGCCCGGGGCATTCGCCGCCGGGCGCCTT
GTTTTGTCTGGTCGCGCGGG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRVTVEAHWLIALSRAGFAEVPR
FSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGA
RDKVIVPALRSVQQRLVALAHALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL
SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIGSST
MPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNED
LDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA

Sequences:

>Translated_479_residues
MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRVTVEAHWLIALSRAGFAEVPR
FSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGA
RDKVIVPALRSVQQRLVALAHALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL
SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIGSST
MPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNED
LDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA
>Mature_479_residues
MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRVTVEAHWLIALSRAGFAEVPR
FSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGA
RDKVIVPALRSVQQRLVALAHALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL
SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIGSST
MPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNED
LDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=454, Percent_Identity=62.9955947136564, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53467; Mature: 53467

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRV
CEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TVEAHWLIALSRAGFAEVPRFSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEY
EEEEHEEEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
WLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGARDKVIVPALRSVQQRLVALA
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
HALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL
HHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWG
ECCCCCCHHHHHHHHHHHHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCCHHH
YIALGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
TDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNEDLDNCWEVLAEPVQTVMRRYG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
IENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRV
CEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TVEAHWLIALSRAGFAEVPRFSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEY
EEEEHEEEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
WLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGARDKVIVPALRSVQQRLVALA
HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
HALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL
HHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWG
ECCCCCCHHHHHHHHHHHHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCCHHH
YIALGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
TDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNEDLDNCWEVLAEPVQTVMRRYG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
IENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]