Click here to switch to the map view.

The map label for this gene is gspO [H]

Identifier: 238026148

GI number: 238026148

Start: 505368

End: 506258

Strand: Reverse

Name: gspO [H]

Synonym: bglu_1g04780

Alternate gene names: 238026148

Gene position: 506258-505368 (Counterclockwise)

Preceding gene: 238026149

Following gene: 238026147

Centisome position: 12.96

GC content: 71.83

Gene sequence:

>891_bases
ATGATCTCGCCCCTCGCCCTGTCCGATCCGTCGCTCGCGTTCGCCGCGTTGCCGCCCGCCGTCCGCTACGTGTTCGCCGC
GGCGATCGGGCTCGTGGTCGGCAGCTTCCTGAACGTGGTGGGGCATCGGCTGCCGGTCATGATGCAGCGCGCCTGGCAGG
CGGAACTCGCCGAGGCGACGGGCGGCGACCCGCCCCCCGACGACGGGCTGCCGGCGCGCTACGACCTGTCGGTGCCGCGC
AGCGCCTGCCCGCATTGCGGCCACGTGCTGCGCGCCTGGGAAAACGTGCCGGTGCTCAGCTACCTGGCGCTGCGCGGCCG
CTGCAGCCGGTGCCGCGCGCCGATCGGCATCCGCTATCCGCTCGTCGAGCTCGTCTCGGGCGCGCTCGCGGTCGCCTCGC
TCGCGGTGTTCGGCCCGGTGCCGGCCGCGCTCGCCGCGTTTGGCCTCTGCGCGATGCTGGTCGCGATGAGCTTGATCGAC
ATCAAGACAGGCTACCTGCCCGATTCGATGACGCTGCCGCTGCTGTGGGCCGGCCTCGTCGTCAATTTGTGGGGCAGCTT
CGTGGACCTGCGCGAGGCAGTGATCGGCGCGATCGCCGGCTATCTGTTCCTCTGGTGCGTGTTCTGGCTGTTCAAGTGGC
TGCGCGGCATCGAGGGCATCGGCTACGGCGACCTGAAGCTGCTCGCCGCGCTAGGCGCCTGGCTCGGCTGGATCGCGCTG
CCGCAGATCCTGCTGGTCGCGGCGCTCAGCGGCGCGGTGGTGGGTCTGGCCGCGACCTGGCGCGGCCGGATGCGCTTCGA
GGAGCCGCTGCCGTTCGGGCCGTTTCTCGCGCTCGGCGGCATTGCGACGCTGCTCTGCGGCACGCCGTTCTATCCGGCGA
TCGGGGGCTGA

Upstream 100 bases:

>100_bases
TGGTCGCGATGTACCTGCCGATCGTTCAACTCGGCAACGTGGTGTAGCATCGCTCCCGGTTGCCGCGCTTCGCTTTCTCC
CTCTTTCCCGAACGGTTTCG

Downstream 100 bases:

>100_bases
TCCTATGCTTTCGATCGGCTTGACGGGCGGCATCGGCAGCGGCAAGACCACCATCGCGAACCGCTTCGGCGAGCGCGGCG
CGAGCCTCGTCGACACCGAC

Product: type IV pilus prepilin peptidase PilD

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MISPLALSDPSLAFAALPPAVRYVFAAAIGLVVGSFLNVVGHRLPVMMQRAWQAELAEATGGDPPPDDGLPARYDLSVPR
SACPHCGHVLRAWENVPVLSYLALRGRCSRCRAPIGIRYPLVELVSGALAVASLAVFGPVPAALAAFGLCAMLVAMSLID
IKTGYLPDSMTLPLLWAGLVVNLWGSFVDLREAVIGAIAGYLFLWCVFWLFKWLRGIEGIGYGDLKLLAALGAWLGWIAL
PQILLVAALSGAVVGLAATWRGRMRFEEPLPFGPFLALGGIATLLCGTPFYPAIGG

Sequences:

>Translated_296_residues
MISPLALSDPSLAFAALPPAVRYVFAAAIGLVVGSFLNVVGHRLPVMMQRAWQAELAEATGGDPPPDDGLPARYDLSVPR
SACPHCGHVLRAWENVPVLSYLALRGRCSRCRAPIGIRYPLVELVSGALAVASLAVFGPVPAALAAFGLCAMLVAMSLID
IKTGYLPDSMTLPLLWAGLVVNLWGSFVDLREAVIGAIAGYLFLWCVFWLFKWLRGIEGIGYGDLKLLAALGAWLGWIAL
PQILLVAALSGAVVGLAATWRGRMRFEEPLPFGPFLALGGIATLLCGTPFYPAIGG
>Mature_296_residues
MISPLALSDPSLAFAALPPAVRYVFAAAIGLVVGSFLNVVGHRLPVMMQRAWQAELAEATGGDPPPDDGLPARYDLSVPR
SACPHCGHVLRAWENVPVLSYLALRGRCSRCRAPIGIRYPLVELVSGALAVASLAVFGPVPAALAAFGLCAMLVAMSLID
IKTGYLPDSMTLPLLWAGLVVNLWGSFVDLREAVIGAIAGYLFLWCVFWLFKWLRGIEGIGYGDLKLLAALGAWLGWIAL
PQILLVAALSGAVVGLAATWRGRMRFEEPLPFGPFLALGGIATLLCGTPFYPAIGG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=272, Percent_Identity=38.2352941176471, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789732, Length=148, Percent_Identity=46.6216216216216, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31363; Mature: 31363

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPLALSDPSLAFAALPPAVRYVFAAAIGLVVGSFLNVVGHRLPVMMQRAWQAELAEAT
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGDPPPDDGLPARYDLSVPRSACPHCGHVLRAWENVPVLSYLALRGRCSRCRAPIGIRYP
CCCCCCCCCCCCCEECCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHH
LVELVSGALAVASLAVFGPVPAALAAFGLCAMLVAMSLIDIKTGYLPDSMTLPLLWAGLV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VNLWGSFVDLREAVIGAIAGYLFLWCVFWLFKWLRGIEGIGYGDLKLLAALGAWLGWIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
PQILLVAALSGAVVGLAATWRGRMRFEEPLPFGPFLALGGIATLLCGTPFYPAIGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MISPLALSDPSLAFAALPPAVRYVFAAAIGLVVGSFLNVVGHRLPVMMQRAWQAELAEAT
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGDPPPDDGLPARYDLSVPRSACPHCGHVLRAWENVPVLSYLALRGRCSRCRAPIGIRYP
CCCCCCCCCCCCCEECCCCHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHH
LVELVSGALAVASLAVFGPVPAALAAFGLCAMLVAMSLIDIKTGYLPDSMTLPLLWAGLV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VNLWGSFVDLREAVIGAIAGYLFLWCVFWLFKWLRGIEGIGYGDLKLLAALGAWLGWIAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
PQILLVAALSGAVVGLAATWRGRMRFEEPLPFGPFLALGGIATLLCGTPFYPAIGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]