BacMap

Definition	Burkholderia glumae BGR1 chromosome chromosome 1, complete sequence.
Accession	NC_012724
Length	3,906,507

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The map label for this gene is fadB1 [H]

Identifier: 238026120

GI number: 238026120

Start: 467957

End: 468733

Strand: Direct

Name: fadB1 [H]

Synonym: bglu_1g04500

Alternate gene names: 238026120

Gene position: 467957-468733 (Clockwise)

Preceding gene: 238026115

Following gene: 238026121

Centisome position: 11.98

GC content: 64.22

Gene sequence:

>777_bases
ATGGCTTATGAAAACCTCCTGGTGGAGACCCGCGGACGTGTCGGGCTGATCACGCTGCATCGTCCGAAGGCGCTGAACGC
ATTGAACGACGCCCTGATGGACGAACTCGGTGCGGCGCTCAAAGCGTTCGACGCCGACGAAGCGATCGGCGCGATCGTGC
TGACCGGTAGCGAGAAGGCATTCGCGGCCGGCGCCGACATCGGGATGATGGCCAACTACTCGTATATGGACGTCTACAAG
GGCGACTACATCACCCGCAACTGGGAGACGATCCGGCAGATCCGCAAGCCGATCCTCGCGGCCGTGGCGGGGTTCGCACT
GGGCGGCGGCTGCGAGCTCGCGATGATGTGCGACATCATCATCGCGGCCGACACGGCGAAGTTCGGCCAGCCCGAGATCC
GGCTCGGCGTGATGCCAGGCGCGGGTGGTACGCAGCGGCTGCCGCGCGCGGTGTCGAAGGCCAAGGCCATGGATATGTGC
CTGACCGCGCGCTTCATGGAGGCCGAAGAGGCGGAGCGCGCCGGGCTCGTGTCACGCGTGGTGCCGGCCGCCTCGTTGCT
CGATGAGGCGCTCGCGGCTGCCACGACGATTGCCGAATTCTCGCTGCCGGCAGTGATGATGGTGAAGGAGTCGGTCAATC
GTGCCTATGAGACGACGCTTTCGGAGGGCGTGCATTTCGAACGGCGCGTGTTCCATTCGATCTTCGCCACCGAGGATCAG
AAGGAAGGCATGGCGGCCTTCGTCGAGAAACGCAAGCCCGTGTTCAAACATCGTTGA

Upstream 100 bases:

>100_bases
GCGGACGTAATTAATTAACCGACCGGTTGGTCGGCAAATGTTAGCATCCTTCCCGGCGCGTGTGCGCAATGAATGACGTT
TCTTTGATGAGGAGACAGCG

Downstream 100 bases:

>100_bases
GCACGATGGCGTGTGCCATGATGGTTGCGTCCGCAACCTGATTGCGCGCCTCCCCGTTTCCGATCAAGGGCTTGGACGAC
CAAGGGCGCCGCGCGAGCGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MAYENLLVETRGRVGLITLHRPKALNALNDALMDELGAALKAFDADEAIGAIVLTGSEKAFAAGADIGMMANYSYMDVYK
GDYITRNWETIRQIRKPILAAVAGFALGGGCELAMMCDIIIAADTAKFGQPEIRLGVMPGAGGTQRLPRAVSKAKAMDMC
LTARFMEAEEAERAGLVSRVVPAASLLDEALAAATTIAEFSLPAVMMVKESVNRAYETTLSEGVHFERRVFHSIFATEDQ
KEGMAAFVEKRKPVFKHR

Sequences:

>Translated_258_residues
MAYENLLVETRGRVGLITLHRPKALNALNDALMDELGAALKAFDADEAIGAIVLTGSEKAFAAGADIGMMANYSYMDVYK
GDYITRNWETIRQIRKPILAAVAGFALGGGCELAMMCDIIIAADTAKFGQPEIRLGVMPGAGGTQRLPRAVSKAKAMDMC
LTARFMEAEEAERAGLVSRVVPAASLLDEALAAATTIAEFSLPAVMMVKESVNRAYETTLSEGVHFERRVFHSIFATEDQ
KEGMAAFVEKRKPVFKHR
>Mature_257_residues
AYENLLVETRGRVGLITLHRPKALNALNDALMDELGAALKAFDADEAIGAIVLTGSEKAFAAGADIGMMANYSYMDVYKG
DYITRNWETIRQIRKPILAAVAGFALGGGCELAMMCDIIIAADTAKFGQPEIRLGVMPGAGGTQRLPRAVSKAKAMDMCL
TARFMEAEEAERAGLVSRVVPAASLLDEALAAATTIAEFSLPAVMMVKESVNRAYETTLSEGVHFERRVFHSIFATEDQK
EGMAAFVEKRKPVFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=55.984555984556, Blast_Score=295, Evalue=3e-80,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=35.15625, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=30.6122448979592, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.9548872180451, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI68989263, Length=227, Percent_Identity=31.7180616740088, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=37.2881355932203, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=31.5573770491803, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI37594471, Length=214, Percent_Identity=28.9719626168224, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI37594469, Length=214, Percent_Identity=28.9719626168224, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI45643119, Length=246, Percent_Identity=27.6422764227642, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260275230, Length=246, Percent_Identity=27.6422764227642, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI260274832, Length=246, Percent_Identity=27.6422764227642, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=24.9027237354086, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=24.9027237354086, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=25, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=25, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4757966, Length=249, Percent_Identity=24.8995983935743, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538581, Length=249, Percent_Identity=24.8995983935743, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136756, Length=232, Percent_Identity=26.2931034482759, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221136753, Length=232, Percent_Identity=26.2931034482759, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221307494, Length=232, Percent_Identity=26.2931034482759, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI213417737, Length=234, Percent_Identity=22.2222222222222, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI157694516, Length=234, Percent_Identity=22.2222222222222, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=32.5925925925926, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI116235487, Length=183, Percent_Identity=28.9617486338798, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=44.2687747035573, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=37.3076923076923, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=35.0806451612903, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=32.0158102766798, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=31.9230769230769, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788682, Length=207, Percent_Identity=31.8840579710145, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790281, Length=175, Percent_Identity=30.2857142857143, Blast_Score=87, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=54.1832669322709, Blast_Score=283, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=53.9094650205761, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=34.251968503937, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=32.9113924050633, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=29.5964125560538, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=184, Percent_Identity=36.9565217391304, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=36.2637362637363, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=36.2637362637363, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=36.2637362637363, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.5625, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=27.9245283018868, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=218, Percent_Identity=29.3577981651376, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=218, Percent_Identity=29.3577981651376, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=213, Percent_Identity=30.0469483568075, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=263, Percent_Identity=25.4752851711027, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=238, Percent_Identity=25.2100840336134, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=29.8429319371728, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=57.3643410852713, Blast_Score=294, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=57.3643410852713, Blast_Score=294, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=37.3540856031128, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=32.3076923076923, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=33.7254901960784, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=28.8537549407115, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=29.4117647058824, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24583077, Length=189, Percent_Identity=34.3915343915344, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24583079, Length=189, Percent_Identity=34.3915343915344, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19921000, Length=189, Percent_Identity=34.3915343915344, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28571729, Length=230, Percent_Identity=26.5217391304348, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571730, Length=230, Percent_Identity=26.5217391304348, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27940; Mature: 27809

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYENLLVETRGRVGLITLHRPKALNALNDALMDELGAALKAFDADEAIGAIVLTGSEKA
CCCHHHEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADIGMMANYSYMDVYKGDYITRNWETIRQIRKPILAAVAGFALGGGCELAMMCDII
EECCCCCCHHCCCCCCEEECCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IAADTAKFGQPEIRLGVMPGAGGTQRLPRAVSKAKAMDMCLTARFMEAEEAERAGLVSRV
HEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPAASLLDEALAAATTIAEFSLPAVMMVKESVNRAYETTLSEGVHFERRVFHSIFATEDQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYENLLVETRGRVGLITLHRPKALNALNDALMDELGAALKAFDADEAIGAIVLTGSEKA
CCHHHEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADIGMMANYSYMDVYKGDYITRNWETIRQIRKPILAAVAGFALGGGCELAMMCDII
EECCCCCCHHCCCCCCEEECCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IAADTAKFGQPEIRLGVMPGAGGTQRLPRAVSKAKAMDMCLTARFMEAEEAERAGLVSRV
HEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPAASLLDEALAAATTIAEFSLPAVMMVKESVNRAYETTLSEGVHFERRVFHSIFATEDQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
KEGMAAFVEKRKPVFKHR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]