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The map label for this gene is nitA [H]

Identifier: 238025831

GI number: 238025831

Start: 163277

End: 164200

Strand: Reverse

Name: nitA [H]

Synonym: bglu_1g01570

Alternate gene names: 238025831

Gene position: 164200-163277 (Counterclockwise)

Preceding gene: 238025834

Following gene: 238025830

Centisome position: 4.2

GC content: 72.84

Gene sequence:

>924_bases
ATGCCCCAATCCCGCATCGCCGCGCTGCAGATCGGCGCCTCCCCGCTCGGCAAGGCCGAGACCTTGCAGCAGATCCTCGC
CTTCGAGGACGCGATCCGCGCCGAGCGGCTGGCGCTGGTGGTGATGCCCGAGGCGCTGCTGGGCGGCTATCCGAAAGGCG
AGACGTTCGGCACCCGGCTCGGCTACCGGCTGCCCGAAGGACGCGACGCGTTCGCGCGCTACCACGGCAACGCGATCGAC
GTGCCGGGGCCGGAGACCGACGCGCTGGCCGGGCTGTCGGCACGCAGCGGCGCCTCGCTCGTGACCGGCGTGATCGAGCG
CGCCGGCGCGACGCTGTACTGCACGGCGCTGTTCTTCGACCCGGCCGCCGGCCTCGTCGCGGCCCACCGCAAGCTGATGC
CGACCGGCACCGAGCGGCTGATCTGGGGGCAAGGCGACGGCTCGACGCTGCCGGTGGTGGAGACGGCGGCCGGCCGTGCC
GGCGCGGCGATCTGCTGGGAGAACCACATGCCGCTGCTGCGCATGGCGATGTACGCGAAGGGCGTAGAGATCTGGTGCGC
ACCGACCGTGGACGAGCGCGAGATCTGGCAGAGCTCGATGCGCCACATCGCGCACGAGGGGCGCTGCTTCGTGGTGAGCG
CCTGCCAGGTGCAACCGTCGCCGGCCGCGCTCGGCATCGAGGTGCCGGGCTGGGACCCGCAGCGGCCGCTGATCAACGGC
GGCAGCGTGATCGTCGGGCCGCTCGGCGAGGTGCTGGCGGGGCCGCTCGTCGGCGAGACCGGGCTCGTGATCACCGAGGT
CGACACCGCCGAACTCACGCGCGCGCGCTACGACTTCGACGTGGCCGGCCATTACGCGCGCCCCGACGTGTTCACGCTGG
CCGTCGACGAGCGCCCGAAGCGCAGCGTGGTGTTCCACCGGTGA

Upstream 100 bases:

>100_bases
ACGTCGCGGCGCGTTTCGAAGTGCCTGATCGATAACGCAAACCCCACGAACGCACCCGCCCCTGCGATCCAGCGGCTGCG
TCGTCTCACCGGAGTCCGCC

Downstream 100 bases:

>100_bases
GGCGGCGGCCACGCCGCCGCTCGGGCCGCCGGCCGGCTGCGCCCGACCTCGATGGCGCGCCCCGGGCGGCGGAGCGCCCG
AACCCGGCCGCCAGCCGGAT

Product: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPQSRIAALQIGASPLGKAETLQQILAFEDAIRAERLALVVMPEALLGGYPKGETFGTRLGYRLPEGRDAFARYHGNAID
VPGPETDALAGLSARSGASLVTGVIERAGATLYCTALFFDPAAGLVAAHRKLMPTGTERLIWGQGDGSTLPVVETAAGRA
GAAICWENHMPLLRMAMYAKGVEIWCAPTVDEREIWQSSMRHIAHEGRCFVVSACQVQPSPAALGIEVPGWDPQRPLING
GSVIVGPLGEVLAGPLVGETGLVITEVDTAELTRARYDFDVAGHYARPDVFTLAVDERPKRSVVFHR

Sequences:

>Translated_307_residues
MPQSRIAALQIGASPLGKAETLQQILAFEDAIRAERLALVVMPEALLGGYPKGETFGTRLGYRLPEGRDAFARYHGNAID
VPGPETDALAGLSARSGASLVTGVIERAGATLYCTALFFDPAAGLVAAHRKLMPTGTERLIWGQGDGSTLPVVETAAGRA
GAAICWENHMPLLRMAMYAKGVEIWCAPTVDEREIWQSSMRHIAHEGRCFVVSACQVQPSPAALGIEVPGWDPQRPLING
GSVIVGPLGEVLAGPLVGETGLVITEVDTAELTRARYDFDVAGHYARPDVFTLAVDERPKRSVVFHR
>Mature_306_residues
PQSRIAALQIGASPLGKAETLQQILAFEDAIRAERLALVVMPEALLGGYPKGETFGTRLGYRLPEGRDAFARYHGNAIDV
PGPETDALAGLSARSGASLVTGVIERAGATLYCTALFFDPAAGLVAAHRKLMPTGTERLIWGQGDGSTLPVVETAAGRAG
AAICWENHMPLLRMAMYAKGVEIWCAPTVDEREIWQSSMRHIAHEGRCFVVSACQVQPSPAALGIEVPGWDPQRPLINGG
SVIVGPLGEVLAGPLVGETGLVITEVDTAELTRARYDFDVAGHYARPDVFTLAVDERPKRSVVFHR

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=307, Percent_Identity=45.928338762215, Blast_Score=284, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6322027, Length=199, Percent_Identity=59.7989949748744, Blast_Score=242, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6322026, Length=111, Percent_Identity=45.945945945946, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 32804; Mature: 32673

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSRIAALQIGASPLGKAETLQQILAFEDAIRAERLALVVMPEALLGGYPKGETFGTRL
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECHHHHCCCCCCCCHHHHC
GYRLPEGRDAFARYHGNAIDVPGPETDALAGLSARSGASLVTGVIERAGATLYCTALFFD
CCCCCCCCHHHHHHCCCEECCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEC
PAAGLVAAHRKLMPTGTERLIWGQGDGSTLPVVETAAGRAGAAICWENHMPLLRMAMYAK
CHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCCCCCEEEEECCCCHHHHHHHHHC
GVEIWCAPTVDEREIWQSSMRHIAHEGRCFVVSACQVQPSPAALGIEVPGWDPQRPLING
CCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEECC
GSVIVGPLGEVLAGPLVGETGLVITEVDTAELTRARYDFDVAGHYARPDVFTLAVDERPK
CCEEECCCHHHHCCCCCCCCCEEEEECCHHHHHHHHCCEEECCCCCCCCEEEEEECCCCC
RSVVFHR
CCCCCCC
>Mature Secondary Structure 
PQSRIAALQIGASPLGKAETLQQILAFEDAIRAERLALVVMPEALLGGYPKGETFGTRL
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECHHHHCCCCCCCCHHHHC
GYRLPEGRDAFARYHGNAIDVPGPETDALAGLSARSGASLVTGVIERAGATLYCTALFFD
CCCCCCCCHHHHHHCCCEECCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEC
PAAGLVAAHRKLMPTGTERLIWGQGDGSTLPVVETAAGRAGAAICWENHMPLLRMAMYAK
CHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCCCCCEEEEECCCCHHHHHHHHHC
GVEIWCAPTVDEREIWQSSMRHIAHEGRCFVVSACQVQPSPAALGIEVPGWDPQRPLING
CCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEECC
GSVIVGPLGEVLAGPLVGETGLVITEVDTAELTRARYDFDVAGHYARPDVFTLAVDERPK
CCEEECCCHHHHCCCCCCCCCEEEEECCHHHHHHHHCCEEECCCCCCCCEEEEEECCCCC
RSVVFHR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]