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The map label for this gene is outE [H]

Identifier: 238025715

GI number: 238025715

Start: 35030

End: 36514

Strand: Reverse

Name: outE [H]

Synonym: bglu_1g00370

Alternate gene names: 238025715

Gene position: 36514-35030 (Counterclockwise)

Preceding gene: 238025716

Following gene: 238025714

Centisome position: 0.93

GC content: 70.64

Gene sequence:

>1485_bases
GTGAGCGAGCTCGCCAGCGCCGCGCCGGGCGAGCCGGCGGCCCCGTCCGCGGTGGCCGCGCGGCTGCTGCCGTACGGCTT
CGCGCGGGCCGGCCAGATCCTCGTCGCGCAACAGGACGGCGATGCGCTAGAAGTCTGGATCAGCGAGCGCACCACCGATG
CCGCGCTCGCCGAGGTGGCGCGCAACTTCGGCGCGCTGACGGCGCGCCGGCTGCCCGCCGACGAACTGGCGCAGGCCATC
AACACCGCTTATGCGCGCCAGGACGGCAGCGCGGCGCAGGTGGTTGGCGAGGTCGAGGGCGAGGTGGACCTGTCGCGGCT
GATGCAGGACATCCCCGAGGTGGAGGACCTGCTCGAATCGGAGGACGACGCGCCGATCATCCGCATGATCAACGCGCTGC
TCACGCAGGCCGCGCGCGAACAGGCCTCGGACATCCACATCGAGCCCTTCGAGAACGCGTCGGTGGTGCGCTTCCGCGTG
GACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAGGCGCTGCATGGCGCGCTGATCTCGCGGATCAAGATCATGGCGCA
GCTCGACATCGCCGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTCGGCGGCCGTCCGGTGGACGTGCGCG
TCTCGACGCTGCCCACCGGGCACGGCGAGCGCGCGGTGCTGCGTCTGCTGGAAAAGGACGCGCAGCGCCTGAACCTCGAG
GCGCTCGGCATGGGCCGCGACACCCTGGTGCAGTTCGACAAGCTGATCGCGCGCCCGCACGGCATCGTGCTGGTGACCGG
CCCGACCGGCTCGGGCAAGACCACCACGCTCTACGCCGCGATGTCGCGGCTCGAGACCGCGACCACCAACATCATGACGG
TCGAGGATCCGATCGAATACGACCTGTCCGGCATCGGCCAGACCCAGGTCAACGAGAAGATCGGCATGAGTTTCGCGCGT
GCGCTGCGCTCGATCCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAAATCCGCGATCTCGAGACCGCGCAGATCGC
CGTGCAGGCGTCGCTGACGGGCCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGCGTCGGCCGTCACGCGCCTGA
CCGACATGGGCGTGGAGCCGTACCTGCTCGCCTCGTCGCTGCTCGGCGTGCTCGCGCAGCGTCTGGTGCGCCAGCTCTGC
CCGGTCTGCAAGGAGCCGCGCGAGGAAGACGGGCGCACCCTCTATCACCCGGTCGGCTGCGAGAAGTGCGGCCATTCCGG
CTACACCGGCCGGCGCGGCGTATACGAGCTGCTGGTGGTGGACGAGGCGATCCGCGCGCAGATCCACAACAACGCGCCCG
ACTCGGAAATCCTGCGCGCGGGCCGCGAGAACGGCATGCGCACGCTGCGCGACGACGCCGAGCGCTGGCTCGCGGCGGGC
AACACCTCGCTCGAGGAAGTGCTGCGCGTGACGGGAGGCGCATAG

Upstream 100 bases:

>100_bases
CCAGCGCCGGCGCGGCCACGCCGCCGGCCGCCAGCCAGCCCGCGCCGGCCGTGACGACGCCGGTGCCCACGCCGGGCATC
GCCTCGCCGGGAGCGCAGCC

Downstream 100 bases:

>100_bases
CGCGATGCCGGCATTCCGTTTCGAAGCGATCGACGCAGGCGGGCGCCCCCAGAAGGGCGTGATCGAGGCAGACAGCGCGC
GCGCCGCGCGCGGCCAGCTG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSELASAAPGEPAAPSAVAARLLPYGFARAGQILVAQQDGDALEVWISERTTDAALAEVARNFGALTARRLPADELAQAI
NTAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRV
DGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRLNLE
ALGMGRDTLVQFDKLIARPHGIVLVTGPTGSGKTTTLYAAMSRLETATTNIMTVEDPIEYDLSGIGQTQVNEKIGMSFAR
ALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLC
PVCKEPREEDGRTLYHPVGCEKCGHSGYTGRRGVYELLVVDEAIRAQIHNNAPDSEILRAGRENGMRTLRDDAERWLAAG
NTSLEEVLRVTGGA

Sequences:

>Translated_494_residues
MSELASAAPGEPAAPSAVAARLLPYGFARAGQILVAQQDGDALEVWISERTTDAALAEVARNFGALTARRLPADELAQAI
NTAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRV
DGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRLNLE
ALGMGRDTLVQFDKLIARPHGIVLVTGPTGSGKTTTLYAAMSRLETATTNIMTVEDPIEYDLSGIGQTQVNEKIGMSFAR
ALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLC
PVCKEPREEDGRTLYHPVGCEKCGHSGYTGRRGVYELLVVDEAIRAQIHNNAPDSEILRAGRENGMRTLRDDAERWLAAG
NTSLEEVLRVTGGA
>Mature_493_residues
SELASAAPGEPAAPSAVAARLLPYGFARAGQILVAQQDGDALEVWISERTTDAALAEVARNFGALTARRLPADELAQAIN
TAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVD
GTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRLNLEA
LGMGRDTLVQFDKLIARPHGIVLVTGPTGSGKTTTLYAAMSRLETATTNIMTVEDPIEYDLSGIGQTQVNEKIGMSFARA
LRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCP
VCKEPREEDGRTLYHPVGCEKCGHSGYTGRRGVYELLVVDEAIRAQIHNNAPDSEILRAGRENGMRTLRDDAERWLAAGN
TSLEEVLRVTGGA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=449, Percent_Identity=51.8930957683742, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1786296, Length=436, Percent_Identity=42.2018348623853, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI87082188, Length=194, Percent_Identity=43.298969072165, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53481; Mature: 53349

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELASAAPGEPAAPSAVAARLLPYGFARAGQILVAQQDGDALEVWISERTTDAALAEVA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCHHHHHHHHH
RNFGALTARRLPADELAQAINTAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLES
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
EDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGALIS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
RIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRLNLE
HHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHH
ALGMGRDTLVQFDKLIARPHGIVLVTGPTGSGKTTTLYAAMSRLETATTNIMTVEDPIEY
HCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHH
DLSGIGQTQVNEKIGMSFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLAT
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEEEEEE
LHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEPREEDGRTLYHPVGC
EECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCH
EKCGHSGYTGRRGVYELLVVDEAIRAQIHNNAPDSEILRAGRENGMRTLRDDAERWLAAG
HHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
NTSLEEVLRVTGGA
CCCHHHHHHHCCCC
>Mature Secondary Structure 
SELASAAPGEPAAPSAVAARLLPYGFARAGQILVAQQDGDALEVWISERTTDAALAEVA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCHHHHHHHHH
RNFGALTARRLPADELAQAINTAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLES
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
EDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGALIS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
RIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRLNLE
HHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHH
ALGMGRDTLVQFDKLIARPHGIVLVTGPTGSGKTTTLYAAMSRLETATTNIMTVEDPIEY
HCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHH
DLSGIGQTQVNEKIGMSFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLAT
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEEEEEE
LHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEPREEDGRTLYHPVGC
EECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCH
EKCGHSGYTGRRGVYELLVVDEAIRAQIHNNAPDSEILRAGRENGMRTLRDDAERWLAAG
HHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
NTSLEEVLRVTGGA
CCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]