Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 238025331

GI number: 238025331

Start: 2493354

End: 2494892

Strand: Direct

Name: emrB [H]

Synonym: bglu_2g20050

Alternate gene names: 238025331

Gene position: 2493354-2494892 (Clockwise)

Preceding gene: 238025330

Following gene: 238025332

Centisome position: 88.19

GC content: 68.29

Gene sequence:

>1539_bases
ATGGCGCACGTCACTCGGGGGGCGGCGCCGAAACTGCCGCTGGGGCCGATGCAAGGCCCGGCTCTCGTGCTCGTCGCGAT
CGCGATCAGCGTCGCCAACTTCATGCAGACGCTCGATCTGACCATCGCCAACGTCGCGGTACCGACCATCGCCGGCGATC
TGGGCGTCGCGCCCGACATGGGCACCTGGATGCTGACCTCGTTCGCGACGCCGCTCGCCATCACGCTGCCGCTGACCGGC
TGGCTCGCCCAGCGCTTCGGCCAGGTGCGGCTGTTCCGGGTGTCGGTCATGCTGTTCGTGCTGACCTCGATCCTGTGCGG
CCTCGCACCGAATTTCGAATCGCTGCTGCTGTTCCGCGCGCTGCAGGGCGCGGCGTCGGGGCCGATCACCGCACTCTCGC
AAGCACTGCTGATCGCCGTGTTTCCGGCCACGCGCCGCAACGTTGCATTGTCGGTCTGGCAGACCACCACGTTCGTCGCG
CCCGTGCTCGGCCCCATCATCGGCGGCTGGATCACCGACAATCTCAGCTGGCCTTACATCTTCTACGTCAACGTGCCGAC
CGGCGTGCTGGTCCTGCTCGTCCTGACCCGCTTCCTCGGTCAGCGCGACAACCCGACCCGCCACCTTCCCGTCGATGTCG
TCGGGCTGCTGCTGCTCGCCGCCGCATTCGGCTCGCTGCAGCTGATGCTCGACCGCGGCGAGAACCTCGACTGGTTTGCG
GCGCTCGAGATTCGGGTGCTCGCCGTGATCTGCGTCGTGTCGTTCGTCGCGCTGGTGCTCTGGGAACTCACCGATGCGCA
TCCGATCGTCGATCTGCGGCTGTTCGCGGATCGCGGATTCACCACCGGAACGATCGCCGTGACGGTCGGCTTCGGCGTCT
ACTACGGCGCGCTCGTTCTCGTGCCGCTCTGGCTGCAGACCGAGCAGGGCTACACGGCGACCTGGGCCGGCATCGCGACC
GCCCCGCTCGGCATCGCGGGCATCGTGATCGCGCCGCTGATCGGACGCATCCAGGGGCGCAGCGACCCGCGCCACCTCGC
GACCGTGGCGCTGGTCGGCTGGGGCGCGGCATCATTCTGGCGCATGGGCTTCAATACCGACGTGACGCTCGGCGACATCG
CGGCCAACTCGCTGCTGATGGGCGCGGCAACGGCCTTTTTCATCACGCCGCTCGTGTCGCTGTCGATCGCCGGGCTGCCG
CGAGAAAGACTGCCGGCAGCCTCCGGCCTGCAGAACGCGCTGCGCCGGATCGGCACGAGCGTCGCCACCTCGCTCGCGCC
GACCTATTTCGAACGGCGCTCGCGCATTCACCACACCTACCTCGTCGATCGCATCACGCCCTACGATCCGGCCGCCGGCG
ACTGGCTCGCCCGGCTGGAACATGCCGGCATGTCGCCCGCCGGCGCGCTCGCCTCGGTCAGCCGCTCGCTCGACACGCAA
GCCCACATGCTGGCGCTCAACGATTTCTCGTTCGCTTGCCTGCTGTTGTTCGGCGCGACCCTGTTGACCGTCTGGTCCAT
CCGCTACCGGCGTCCGTGA

Upstream 100 bases:

>100_bases
CGATCTTCGACGACGATGCCTCGTCCGGCGATGCGCTCGTCATCGACACGATTCGCGCGAACGACGGTGCGGCCGCCGCG
CGCGCGCCGGGAGCCTGACG

Downstream 100 bases:

>100_bases
CGCCGATCACCCGGTACCTTCCGATGAGCACCGACCTCCAGGATCTCGCCACGCCTCACGCCGCCGCGGCGCGCACGGCC
CGGTTGATCTGCTTTGGCGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAHVTRGAAPKLPLGPMQGPALVLVAIAISVANFMQTLDLTIANVAVPTIAGDLGVAPDMGTWMLTSFATPLAITLPLTG
WLAQRFGQVRLFRVSVMLFVLTSILCGLAPNFESLLLFRALQGAASGPITALSQALLIAVFPATRRNVALSVWQTTTFVA
PVLGPIIGGWITDNLSWPYIFYVNVPTGVLVLLVLTRFLGQRDNPTRHLPVDVVGLLLLAAAFGSLQLMLDRGENLDWFA
ALEIRVLAVICVVSFVALVLWELTDAHPIVDLRLFADRGFTTGTIAVTVGFGVYYGALVLVPLWLQTEQGYTATWAGIAT
APLGIAGIVIAPLIGRIQGRSDPRHLATVALVGWGAASFWRMGFNTDVTLGDIAANSLLMGAATAFFITPLVSLSIAGLP
RERLPAASGLQNALRRIGTSVATSLAPTYFERRSRIHHTYLVDRITPYDPAAGDWLARLEHAGMSPAGALASVSRSLDTQ
AHMLALNDFSFACLLLFGATLLTVWSIRYRRP

Sequences:

>Translated_512_residues
MAHVTRGAAPKLPLGPMQGPALVLVAIAISVANFMQTLDLTIANVAVPTIAGDLGVAPDMGTWMLTSFATPLAITLPLTG
WLAQRFGQVRLFRVSVMLFVLTSILCGLAPNFESLLLFRALQGAASGPITALSQALLIAVFPATRRNVALSVWQTTTFVA
PVLGPIIGGWITDNLSWPYIFYVNVPTGVLVLLVLTRFLGQRDNPTRHLPVDVVGLLLLAAAFGSLQLMLDRGENLDWFA
ALEIRVLAVICVVSFVALVLWELTDAHPIVDLRLFADRGFTTGTIAVTVGFGVYYGALVLVPLWLQTEQGYTATWAGIAT
APLGIAGIVIAPLIGRIQGRSDPRHLATVALVGWGAASFWRMGFNTDVTLGDIAANSLLMGAATAFFITPLVSLSIAGLP
RERLPAASGLQNALRRIGTSVATSLAPTYFERRSRIHHTYLVDRITPYDPAAGDWLARLEHAGMSPAGALASVSRSLDTQ
AHMLALNDFSFACLLLFGATLLTVWSIRYRRP
>Mature_511_residues
AHVTRGAAPKLPLGPMQGPALVLVAIAISVANFMQTLDLTIANVAVPTIAGDLGVAPDMGTWMLTSFATPLAITLPLTGW
LAQRFGQVRLFRVSVMLFVLTSILCGLAPNFESLLLFRALQGAASGPITALSQALLIAVFPATRRNVALSVWQTTTFVAP
VLGPIIGGWITDNLSWPYIFYVNVPTGVLVLLVLTRFLGQRDNPTRHLPVDVVGLLLLAAAFGSLQLMLDRGENLDWFAA
LEIRVLAVICVVSFVALVLWELTDAHPIVDLRLFADRGFTTGTIAVTVGFGVYYGALVLVPLWLQTEQGYTATWAGIATA
PLGIAGIVIAPLIGRIQGRSDPRHLATVALVGWGAASFWRMGFNTDVTLGDIAANSLLMGAATAFFITPLVSLSIAGLPR
ERLPAASGLQNALRRIGTSVATSLAPTYFERRSRIHHTYLVDRITPYDPAAGDWLARLEHAGMSPAGALASVSRSLDTQA
HMLALNDFSFACLLLFGATLLTVWSIRYRRP

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=491, Percent_Identity=42.5661914460285, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1788710, Length=474, Percent_Identity=40.9282700421941, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1790195, Length=323, Percent_Identity=30.3405572755418, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081983, Length=424, Percent_Identity=28.7735849056604, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788392, Length=399, Percent_Identity=26.3157894736842, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1789152, Length=190, Percent_Identity=24.7368421052632, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=241, Percent_Identity=23.6514522821577, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=180, Percent_Identity=25.5555555555556, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=25.3333333333333, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54840; Mature: 54709

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVTRGAAPKLPLGPMQGPALVLVAIAISVANFMQTLDLTIANVAVPTIAGDLGVAPDM
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GTWMLTSFATPLAITLPLTGWLAQRFGQVRLFRVSVMLFVLTSILCGLAPNFESLLLFRA
CHHHHHHHCCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LQGAASGPITALSQALLIAVFPATRRNVALSVWQTTTFVAPVLGPIIGGWITDNLSWPYI
HHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHCCCCCCCEE
FYVNVPTGVLVLLVLTRFLGQRDNPTRHLPVDVVGLLLLAAAFGSLQLMLDRGENLDWFA
EEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
ALEIRVLAVICVVSFVALVLWELTDAHPIVDLRLFADRGFTTGTIAVTVGFGVYYGALVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEHHHHHHHHHHH
VPLWLQTEQGYTATWAGIATAPLGIAGIVIAPLIGRIQGRSDPRHLATVALVGWGAASFW
HHHHHCCCCCCEEEECCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHH
RMGFNTDVTLGDIAANSLLMGAATAFFITPLVSLSIAGLPRERLPAASGLQNALRRIGTS
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
VATSLAPTYFERRSRIHHTYLVDRITPYDPAAGDWLARLEHAGMSPAGALASVSRSLDTQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCH
AHMLALNDFSFACLLLFGATLLTVWSIRYRRP
HHEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AHVTRGAAPKLPLGPMQGPALVLVAIAISVANFMQTLDLTIANVAVPTIAGDLGVAPDM
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GTWMLTSFATPLAITLPLTGWLAQRFGQVRLFRVSVMLFVLTSILCGLAPNFESLLLFRA
CHHHHHHHCCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LQGAASGPITALSQALLIAVFPATRRNVALSVWQTTTFVAPVLGPIIGGWITDNLSWPYI
HHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHCCCCCCCEE
FYVNVPTGVLVLLVLTRFLGQRDNPTRHLPVDVVGLLLLAAAFGSLQLMLDRGENLDWFA
EEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
ALEIRVLAVICVVSFVALVLWELTDAHPIVDLRLFADRGFTTGTIAVTVGFGVYYGALVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEHHHHHHHHHHH
VPLWLQTEQGYTATWAGIATAPLGIAGIVIAPLIGRIQGRSDPRHLATVALVGWGAASFW
HHHHHCCCCCCEEEECCHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHH
RMGFNTDVTLGDIAANSLLMGAATAFFITPLVSLSIAGLPRERLPAASGLQNALRRIGTS
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHH
VATSLAPTYFERRSRIHHTYLVDRITPYDPAAGDWLARLEHAGMSPAGALASVSRSLDTQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCH
AHMLALNDFSFACLLLFGATLLTVWSIRYRRP
HHEEEECCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]