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The map label for this gene is ynfL [H]

Identifier: 238025235

GI number: 238025235

Start: 2377390

End: 2378406

Strand: Direct

Name: ynfL [H]

Synonym: bglu_2g19070

Alternate gene names: 238025235

Gene position: 2377390-2378406 (Clockwise)

Preceding gene: 238025232

Following gene: 238025236

Centisome position: 84.09

GC content: 75.42

Gene sequence:

>1017_bases
ATGGAATCCGCCCGCATCCTCAAGTACTTCCTGGCCGTGGCCGACGCCGGCAGCGTGACCGGCGCGGCCGAGCGGCTCGG
GCTCGCGCAGCCGGCGCTGAGCCAGGCCATCACGCGCTTCGAGCGCGAGCTCGGCGTGCGCCTGTTCTCGCGCACGCGCC
GCGGCGCGGCCCTGACGGCCGCCGGCGAGGCGATCGTCGACGACCTGCGCCTGAGCATGGCGCGCATCGAGGCAGCCGAG
CGGCGCGCGCGCGATATCGGCGCGCAGCGCGGCGGGCGGCTCACGATCGGCTTCGCCTCGGCCGCGCTGTTCGAGCTCAT
GCCGAGGGCGATTGCCGCGCTGCGTGACGAGGTGCCGAACGTCGAGCTGACGCTGCGCGAGATGAGCAATGCCGAGCAGG
CCGGCGCGCTCGAATCGGGCGAGATCGACATCGGCCTGCTGCATACGCCGGTGGCGGTCCGCGGGCGCATGCACGAGAAG
CTGATCGCACGCGAGCGGCTGCAGGCGGTGGTACACGAATCGCATCCGCTCGGCGCGGACGGCCGGCTCGCGCTGGCCGA
TCTCGCCCGCCTCGGCCTCGTCTGGTTCCCGCAGGAGCAGTTGCCGGCGATCCGCGCCGGCATCCTGAGCGCGTTCCGCA
AGGCCGGCTGCGAGACCAGCGTGGTGCAGGACGCGAACCGCTCGCTGACGGTGCTGGCCTGCGTCGCGGCAGGCTGCGGC
GCCTCGCTGCTGCCGCGTTCGGTGCAGGCGCTGCGCTTTTCGGGGGTGCGCGTCTGCGAGCTGCGCGACGGCGCCGCGCT
GCCGTTCTTCGAACTCAGCGCGGTATGGCCCAAGCGGTCGCGGCCGACCCTGGCGGACCGCTTCGCGGCCCTGCTCGAGC
AGGACGACGCCCCAGCGGACGGGCGGGCGGGAGCGAACACGGGCGGGCGGCGGGACGACCAGCCCGCCGCGCCGGGCGGC
GGCTCGGCGAAGCCCGCGGCCCGGCGCCCGGGCCGCGGCACGCACCGGCAGCGGTGA

Upstream 100 bases:

>100_bases
ACGCCGGCGCCCGACCGCGATTGAATGATGGTACGCATTCTGGTGCGGGACGCAGTGCCGTGCATACTATGGAAACGTTG
ATCTTTCATTGCATGGCCTT

Downstream 100 bases:

>100_bases
GCGCGGCGGGCCGGCGCTCGCCGTCGTGCCTCGGCCCGACTGCGTCCACGCCGCGCGATCGCTCCGCCCGGGCCCTCGGC
CGCATCGCGACGCGGCCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MESARILKYFLAVADAGSVTGAAERLGLAQPALSQAITRFERELGVRLFSRTRRGAALTAAGEAIVDDLRLSMARIEAAE
RRARDIGAQRGGRLTIGFASAALFELMPRAIAALRDEVPNVELTLREMSNAEQAGALESGEIDIGLLHTPVAVRGRMHEK
LIARERLQAVVHESHPLGADGRLALADLARLGLVWFPQEQLPAIRAGILSAFRKAGCETSVVQDANRSLTVLACVAAGCG
ASLLPRSVQALRFSGVRVCELRDGAALPFFELSAVWPKRSRPTLADRFAALLEQDDAPADGRAGANTGGRRDDQPAAPGG
GSAKPAARRPGRGTHRQR

Sequences:

>Translated_338_residues
MESARILKYFLAVADAGSVTGAAERLGLAQPALSQAITRFERELGVRLFSRTRRGAALTAAGEAIVDDLRLSMARIEAAE
RRARDIGAQRGGRLTIGFASAALFELMPRAIAALRDEVPNVELTLREMSNAEQAGALESGEIDIGLLHTPVAVRGRMHEK
LIARERLQAVVHESHPLGADGRLALADLARLGLVWFPQEQLPAIRAGILSAFRKAGCETSVVQDANRSLTVLACVAAGCG
ASLLPRSVQALRFSGVRVCELRDGAALPFFELSAVWPKRSRPTLADRFAALLEQDDAPADGRAGANTGGRRDDQPAAPGG
GSAKPAARRPGRGTHRQR
>Mature_338_residues
MESARILKYFLAVADAGSVTGAAERLGLAQPALSQAITRFERELGVRLFSRTRRGAALTAAGEAIVDDLRLSMARIEAAE
RRARDIGAQRGGRLTIGFASAALFELMPRAIAALRDEVPNVELTLREMSNAEQAGALESGEIDIGLLHTPVAVRGRMHEK
LIARERLQAVVHESHPLGADGRLALADLARLGLVWFPQEQLPAIRAGILSAFRKAGCETSVVQDANRSLTVLACVAAGCG
ASLLPRSVQALRFSGVRVCELRDGAALPFFELSAVWPKRSRPTLADRFAALLEQDDAPADGRAGANTGGRRDDQPAAPGG
GSAKPAARRPGRGTHRQR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=284, Percent_Identity=32.7464788732394, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=30.8474576271186, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI157672245, Length=276, Percent_Identity=32.2463768115942, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=32.7935222672065, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI2367136, Length=261, Percent_Identity=29.5019157088123, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=27.5720164609054, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790399, Length=243, Percent_Identity=29.2181069958848, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081904, Length=233, Percent_Identity=30.0429184549356, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693105, Length=140, Percent_Identity=29.2857142857143, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787601, Length=137, Percent_Identity=32.1167883211679, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=24.1935483870968, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789204, Length=268, Percent_Identity=24.6268656716418, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=24.1935483870968, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35997; Mature: 35997

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESARILKYFLAVADAGSVTGAAERLGLAQPALSQAITRFERELGVRLFSRTRRGAALTA
CCHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHH
AGEAIVDDLRLSMARIEAAERRARDIGAQRGGRLTIGFASAALFELMPRAIAALRDEVPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCC
VELTLREMSNAEQAGALESGEIDIGLLHTPVAVRGRMHEKLIARERLQAVVHESHPLGAD
CEEEHHHHCCHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GRLALADLARLGLVWFPQEQLPAIRAGILSAFRKAGCETSVVQDANRSLTVLACVAAGCG
CCHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCC
ASLLPRSVQALRFSGVRVCELRDGAALPFFELSAVWPKRSRPTLADRFAALLEQDDAPAD
HHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCC
GRAGANTGGRRDDQPAAPGGGSAKPAARRPGRGTHRQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MESARILKYFLAVADAGSVTGAAERLGLAQPALSQAITRFERELGVRLFSRTRRGAALTA
CCHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHH
AGEAIVDDLRLSMARIEAAERRARDIGAQRGGRLTIGFASAALFELMPRAIAALRDEVPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCC
VELTLREMSNAEQAGALESGEIDIGLLHTPVAVRGRMHEKLIARERLQAVVHESHPLGAD
CEEEHHHHCCHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GRLALADLARLGLVWFPQEQLPAIRAGILSAFRKAGCETSVVQDANRSLTVLACVAAGCG
CCHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCC
ASLLPRSVQALRFSGVRVCELRDGAALPFFELSAVWPKRSRPTLADRFAALLEQDDAPAD
HHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCC
GRAGANTGGRRDDQPAAPGGGSAKPAARRPGRGTHRQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]