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The map label for this gene is hpaC [H]

Identifier: 238025091

GI number: 238025091

Start: 2193101

End: 2193634

Strand: Direct

Name: hpaC [H]

Synonym: bglu_2g17480

Alternate gene names: 238025091

Gene position: 2193101-2193634 (Clockwise)

Preceding gene: 238025090

Following gene: 238025098

Centisome position: 77.57

GC content: 73.41

Gene sequence:

>534_bases
ATGACCGACCCTTCGCGCGCGAGTGACGCCGACGATGCCCGCACGCTGTTCCGCCACGCGATGGCGCATCTGGGCGCCGC
CGTCAACGTGATCACGACGGCCGGCCCGCGCGGCCGCTGCGGCGTGACCGCGAGCGCCGTCTGCTCGGTCACCGACTCGC
CGCCGACGCTGCTCGTCTGCCTGAACCGCAGCAGCAGCACGCACGCGGCGTTCACGGCCCACCGCTCGCTCTGCGTGAAC
GTGCTGCCGGCCGAGCACGAGCCGCTCGCGCGCCACTTCGCGGGCCTGACCAACCTGCCGATGGACGCGCGCTTCGGCCA
GACCGGCTGGGACGAGGGCCCCGGCGGCCTGCCGGTGCTGCGCGACGCGCTGGCGAGCGTGCAGGGCACCATCGTCGAGA
TGAAGGAGGTGGGCTCGCACACGGTGCTGTTCGTCGAGGCGACGGCCATCCGCGTGCGCGGCGAAGGCGACGCGCTGATC
TACTTCGGGCGCGCGTTCCATCGCGTCGGCAGGGCCGTGGCCAGGCAGGACTGA

Upstream 100 bases:

>100_bases
CGAGCCGACGCGCGACACTGCCGGTCCGGCCGGCGAATTCCGCGGCGCGGTATTCCAGGCCATCCGTCGTTTCGTTCTGC
TTTCCGTTTCAGGAGTTCTC

Downstream 100 bases:

>100_bases
ACCCAGGGGCATGGCCCGCTGCGTTTCGATCCGGGTCTCGCCCAGCCGTGCCGTCAGGGCGCGAACTTCCCGAACCTCGG
CGCCCGCGCGGCCGGCACCA

Product: 4-hydroxyphenylacetate 3-monooxygenase reductase subunit

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-monooxygenase small component; Flavin:NADH reductase [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MTDPSRASDADDARTLFRHAMAHLGAAVNVITTAGPRGRCGVTASAVCSVTDSPPTLLVCLNRSSSTHAAFTAHRSLCVN
VLPAEHEPLARHFAGLTNLPMDARFGQTGWDEGPGGLPVLRDALASVQGTIVEMKEVGSHTVLFVEATAIRVRGEGDALI
YFGRAFHRVGRAVARQD

Sequences:

>Translated_177_residues
MTDPSRASDADDARTLFRHAMAHLGAAVNVITTAGPRGRCGVTASAVCSVTDSPPTLLVCLNRSSSTHAAFTAHRSLCVN
VLPAEHEPLARHFAGLTNLPMDARFGQTGWDEGPGGLPVLRDALASVQGTIVEMKEVGSHTVLFVEATAIRVRGEGDALI
YFGRAFHRVGRAVARQD
>Mature_176_residues
TDPSRASDADDARTLFRHAMAHLGAAVNVITTAGPRGRCGVTASAVCSVTDSPPTLLVCLNRSSSTHAAFTAHRSLCVNV
LPAEHEPLARHFAGLTNLPMDARFGQTGWDEGPGGLPVLRDALASVQGTIVEMKEVGSHTVLFVEATAIRVRGEGDALIY
FGRAFHRVGRAVARQD

Specific function: Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the large hpaB component or to other electron acceptors such as cytochrome c and Fe(3+) ion [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. HpaC subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=155, Percent_Identity=50.3225806451613, Blast_Score=148, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR011982
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.14.13.3

Molecular weight: Translated: 18727; Mature: 18596

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPSRASDADDARTLFRHAMAHLGAAVNVITTAGPRGRCGVTASAVCSVTDSPPTLLVC
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHEEEECCCCCEEEEE
LNRSSSTHAAFTAHRSLCVNVLPAEHEPLARHFAGLTNLPMDARFGQTGWDEGPGGLPVL
ECCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
RDALASVQGTIVEMKEVGSHTVLFVEATAIRVRGEGDALIYFGRAFHRVGRAVARQD
HHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDPSRASDADDARTLFRHAMAHLGAAVNVITTAGPRGRCGVTASAVCSVTDSPPTLLVC
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHEEEECCCCCEEEEE
LNRSSSTHAAFTAHRSLCVNVLPAEHEPLARHFAGLTNLPMDARFGQTGWDEGPGGLPVL
ECCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
RDALASVQGTIVEMKEVGSHTVLFVEATAIRVRGEGDALIYFGRAFHRVGRAVARQD
HHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10633095 [H]