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The map label for this gene is narK2 [H]

Identifier: 238025087

GI number: 238025087

Start: 2187773

End: 2189062

Strand: Direct

Name: narK2 [H]

Synonym: bglu_2g17430

Alternate gene names: 238025087

Gene position: 2187773-2189062 (Clockwise)

Preceding gene: 238025086

Following gene: 238025089

Centisome position: 77.38

GC content: 69.3

Gene sequence:

>1290_bases
ATGACTCGTCAACTCAACCCCGGCTCGTCGCCGGATGCGCAGAAGATCCCACTGCGCGCATGGTCGGTGCTCTTCGCGAG
CACGTTCGCATTCATGGTCTGTTTCGTCGTCTGGATGATGTTCGGCGTGCTGGGGGTGCAACTGCGCACCGATCTCGCGC
TCAACAGCTTCGAATTCGGCCTGCTGACCGCCACGCCGGTGCTGACGGGCGCCGTGATGCGCCTGCCGCTCGGCATCTGG
ACCGACCGCTTCGGCGGGCGCGTGGTGATGACCACGCTGCTGGTCGGCTGCGCGGTGCCGGTGTACGGCATCAGCTATGC
GACGCAGTTCTGGCAGTTCCTCGTGATCGGCCTGTTCCTCGGCTGCGTGGGCGCCTCGTTCGCGGTCGGCACGCCCTACG
TGGCGCGCTTCTTCCCGCCCAAGCGGCGCGGCTTCGCGATGGGCTTCTTCGGCGCGGGCACGGTGGGGGCGGCCGTCAAC
CTGTTCGTCACGCCGTCGCTCGAAGTGGCCTACGGCTGGCGCTTCGTGCCGCGCGCGTATGCGGTGGTGCTGCTCGTCAC
GGCGGCGATCTTCTGGTTCGCCTCGGCGCGCGACCCCGGCGCGGGCGCCAGGCGCGGCTCGATACTCGCCTCGTTCCGCA
CGCTGCGCGACCCGCGCGTCTGGCGGCTTTGCCAGTATTACTCGGTCACGTTCGGCGGCTTCACGGCGCTCTCGCTCTGG
ATCCCGCAGTACGTCACGCGCGAATACAGCGTCTCGCTGGTGGCCGCCTCGGCCTTCGCCGCCGGCTTCTCGCTGCCGGG
CTCGGTGCTGCGCGCGCTCGGCGGCGCGCTGTCGGACCGCTTCGGCGCCCACGCGGTGACCTGGCACGGCCTGTGGGTCG
CCTGGATCTGCCTGTTCCTGCTGTCGTATCCGACCACCGAGTTCGTGATCCGCACCATCGACGGCACGGCGCGGCTGCAC
ATCGCCATGCCGCTCGCCGGCTTCCTCGTGCTGACCTTCGTGCTCGGCGTGGTGTTCGCGTTCGGCATGGCCTCCACCTT
CAAGTACGTCGCCGACGATTTCCCCGACCACATGGGCGTGGTGACCGGCATCGTCGGGCTGGCGGGCGGCCTCGGCGGCT
TCCTGCTGCCGGTGATGTGGGGCGCGCTGCTCGAATTCATCCCCGTGCGCACCACCTGCTTCATGCTGCTCTACGGCATC
GTATGGGTGTCGCTGATCCTGCTCTACGTGTCCGGCGTGCGGCGCACGCCGCTGACCGGCGCGGTGCAGCCCGCGGGCAA
GGGCGGTTGA

Upstream 100 bases:

>100_bases
CCCCGCCCGACTCTGCCCTGCCCGAAATCGAGCCGGCCGCCGCGCCGCGGACGCGGTGCTGCCCCGGCGACTCTCGCCTC
AATCACACGGAGTGTCGTGT

Downstream 100 bases:

>100_bases
GCGGGCACGCGAGCGCACCGGCGATCGTCCGGCGGCAACGCAGGGCCCCGATCCTCCGGATCTCGACCCTGCCTACGACG
ATCGCGCATCACGCATCGCG

Product: nitrate/nitrite transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTRQLNPGSSPDAQKIPLRAWSVLFASTFAFMVCFVVWMMFGVLGVQLRTDLALNSFEFGLLTATPVLTGAVMRLPLGIW
TDRFGGRVVMTTLLVGCAVPVYGISYATQFWQFLVIGLFLGCVGASFAVGTPYVARFFPPKRRGFAMGFFGAGTVGAAVN
LFVTPSLEVAYGWRFVPRAYAVVLLVTAAIFWFASARDPGAGARRGSILASFRTLRDPRVWRLCQYYSVTFGGFTALSLW
IPQYVTREYSVSLVAASAFAAGFSLPGSVLRALGGALSDRFGAHAVTWHGLWVAWICLFLLSYPTTEFVIRTIDGTARLH
IAMPLAGFLVLTFVLGVVFAFGMASTFKYVADDFPDHMGVVTGIVGLAGGLGGFLLPVMWGALLEFIPVRTTCFMLLYGI
VWVSLILLYVSGVRRTPLTGAVQPAGKGG

Sequences:

>Translated_429_residues
MTRQLNPGSSPDAQKIPLRAWSVLFASTFAFMVCFVVWMMFGVLGVQLRTDLALNSFEFGLLTATPVLTGAVMRLPLGIW
TDRFGGRVVMTTLLVGCAVPVYGISYATQFWQFLVIGLFLGCVGASFAVGTPYVARFFPPKRRGFAMGFFGAGTVGAAVN
LFVTPSLEVAYGWRFVPRAYAVVLLVTAAIFWFASARDPGAGARRGSILASFRTLRDPRVWRLCQYYSVTFGGFTALSLW
IPQYVTREYSVSLVAASAFAAGFSLPGSVLRALGGALSDRFGAHAVTWHGLWVAWICLFLLSYPTTEFVIRTIDGTARLH
IAMPLAGFLVLTFVLGVVFAFGMASTFKYVADDFPDHMGVVTGIVGLAGGLGGFLLPVMWGALLEFIPVRTTCFMLLYGI
VWVSLILLYVSGVRRTPLTGAVQPAGKGG
>Mature_428_residues
TRQLNPGSSPDAQKIPLRAWSVLFASTFAFMVCFVVWMMFGVLGVQLRTDLALNSFEFGLLTATPVLTGAVMRLPLGIWT
DRFGGRVVMTTLLVGCAVPVYGISYATQFWQFLVIGLFLGCVGASFAVGTPYVARFFPPKRRGFAMGFFGAGTVGAAVNL
FVTPSLEVAYGWRFVPRAYAVVLLVTAAIFWFASARDPGAGARRGSILASFRTLRDPRVWRLCQYYSVTFGGFTALSLWI
PQYVTREYSVSLVAASAFAAGFSLPGSVLRALGGALSDRFGAHAVTWHGLWVAWICLFLLSYPTTEFVIRTIDGTARLHI
AMPLAGFLVLTFVLGVVFAFGMASTFKYVADDFPDHMGVVTGIVGLAGGLGGFLLPVMWGALLEFIPVRTTCFMLLYGIV
WVSLILLYVSGVRRTPLTGAVQPAGKGG

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=441, Percent_Identity=27.2108843537415, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787475, Length=302, Percent_Identity=26.4900662251656, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46380; Mature: 46249

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQLNPGSSPDAQKIPLRAWSVLFASTFAFMVCFVVWMMFGVLGVQLRTDLALNSFEFG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LLTATPVLTGAVMRLPLGIWTDRFGGRVVMTTLLVGCAVPVYGISYATQFWQFLVIGLFL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCVGASFAVGTPYVARFFPPKRRGFAMGFFGAGTVGAAVNLFVTPSLEVAYGWRFVPRAY
HHHHCCHHCCCCHHHHCCCCCCCCCEEEECCCCCHHHEEEEEECCCEEHHHCCCHHHHHH
AVVLLVTAAIFWFASARDPGAGARRGSILASFRTLRDPRVWRLCQYYSVTFGGFTALSLW
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHH
IPQYVTREYSVSLVAASAFAAGFSLPGSVLRALGGALSDRFGAHAVTWHGLWVAWICLFL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHH
LSYPTTEFVIRTIDGTARLHIAMPLAGFLVLTFVLGVVFAFGMASTFKYVADDFPDHMGV
HHCCCHHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VTGIVGLAGGLGGFLLPVMWGALLEFIPVRTTCFMLLYGIVWVSLILLYVSGVRRTPLTG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AVQPAGKGG
CCCCCCCCC
>Mature Secondary Structure 
TRQLNPGSSPDAQKIPLRAWSVLFASTFAFMVCFVVWMMFGVLGVQLRTDLALNSFEFG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
LLTATPVLTGAVMRLPLGIWTDRFGGRVVMTTLLVGCAVPVYGISYATQFWQFLVIGLFL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCVGASFAVGTPYVARFFPPKRRGFAMGFFGAGTVGAAVNLFVTPSLEVAYGWRFVPRAY
HHHHCCHHCCCCHHHHCCCCCCCCCEEEECCCCCHHHEEEEEECCCEEHHHCCCHHHHHH
AVVLLVTAAIFWFASARDPGAGARRGSILASFRTLRDPRVWRLCQYYSVTFGGFTALSLW
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHH
IPQYVTREYSVSLVAASAFAAGFSLPGSVLRALGGALSDRFGAHAVTWHGLWVAWICLFL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHH
LSYPTTEFVIRTIDGTARLHIAMPLAGFLVLTFVLGVVFAFGMASTFKYVADDFPDHMGV
HHCCCHHHEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VTGIVGLAGGLGGFLLPVMWGALLEFIPVRTTCFMLLYGIVWVSLILLYVSGVRRTPLTG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AVQPAGKGG
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]