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The map label for this gene is narY [H]

Identifier: 238025084

GI number: 238025084

Start: 2184544

End: 2186082

Strand: Direct

Name: narY [H]

Synonym: bglu_2g17400

Alternate gene names: 238025084

Gene position: 2184544-2186082 (Clockwise)

Preceding gene: 238025083

Following gene: 238025085

Centisome position: 77.27

GC content: 68.75

Gene sequence:

>1539_bases
ATGAAAATCCGTGCCCAGATCGCGATGGTGCTGAACCTCGACAAATGCATCGGCTGCCACACCTGCTCGGTCACCTGCAA
GAACGTCTGGACGAGCCGCGAAGGCGTGGAATACGCCTGGTTCAACAACGTCGAGTCCAAGCCCGGCGTCGGCTATCCGA
ACGAATGGGAAAACCAGCGGCGCTGGCACGGCGGCTGGCAACGCCACGCCGACGGCCGCCTCACGCCGCGCGGCGGCAAC
CGGCTGCGCGTGCTCGCGAAACTCTTCGCGAACCCCGACCTGCCCCAGATCGACGACTACTACGAGCCGTTCACCTTCGA
CTACGCGCATCTGCACGACGCCGGCCCCACCGGCGCGCCGCCGGTGGCAAGGCCGCGCTCGCTGGTCAGCGGCCGGCGGC
TCGACAAGATCGTGGGCGGGCCGAACTGGGAGGAGATCCTCGGCGGCGAGTTCGACAAGCGCGCCCGCGATGCGAACTTC
GAGGCCGTGCAGAAGGAGATCTACGGCCAGTTCGAAAACACCTTCATGATGTACCTGCCGCGGCTCTGCGAGCACTGCCT
GAACCCGGCCTGCGTCGCGTCGTGCCCGTCGGGCTCGATCTACAAGCGCGAGGAGGACGGCGTGGTGCTGATCGACCAGG
ACAAGTGCCGCGGCTGGCGCATGTGCGTGTCGGGCTGCCCGTACAAGAAGATCTACTACAACTGGAAGAGCGGCAAGGCC
GAGAAGTGCATCTTCTGCTATCCGCGCATCGAGGCCGGCGAGCCGACCGTCTGCTCGGAAACCTGCGTGGGCCGCATCCG
CTATCTCGGCGTGCTGCTCTACGACGCCGACCGGATCGGCGAGGCCGCCGCCGTCGAGCGCGAGACCGATCTCTACGAGG
CGCAGTGCTCGATGTTCCTCGATCCGCACGATCCGCTCGTGATCGCGCAGGCCGAGCGCGACGGCGTGCCGGCCGCCTGG
CTCGAGGCGGCGCGCCGCTCGCCCGTCTACAAGATGGCGATCGACTGGAAGATCGCGTTTCCGCTGCACCCGGAATACCG
CACGCTGCCGATGGTCTGGTACGTGCCGCCGCTCTCGCCGATCAACGCGGCGGCCAACGCCGGCGCGCTCGGCATGCACG
GCCTGCTGCCCGACGTGGGCGCGCTGCGCATCCCGCTGCGCTACCTCGCGAACCTGCTGACGGCAGGCGACGAGGCGCCG
GTGAAGCTCGCGCTCGAACGGCTGCTGGCGATGCGCGGCTTCATGCGCTCGCGCCACGTGGACGGCGCCAGCGACGCCGA
GGCGCGCGCCGTGCTCGACCAGGCCGGCCTGACGGTCGCGCAGGTCGAGGAGATGTACCGCTACCTCGCGATCGCCAACC
ACGAGGACCGCTTCGTGATCCCGAGCAGCCACCGCGAATACGCGGAGGACACCTTCGACCTGCGCGCCGCCTGCGGCTTC
TCGTTCGGCAACGGCTGCTCGGACGGGCGCTCCGACACCGGCCTGTTCGGCTCGGCGCGGCCGCGCAAGACCATCCCGAT
CCGCGAGGTGACGCCATGA

Upstream 100 bases:

>100_bases
GCTCGAACCGCGACGAGTTCCTGATCGTGCGCAAGATGAACCGTATCGACTGGCTCGATGGCGAGACCGCCACGGCCAGC
CAGCCTGCCGGAGAAACACG

Downstream 100 bases:

>100_bases
GCCCGACGCCGTCCGACACGGTGCAGCCGGTCGCCTACGCGGCGCTGTCGATGCTGCTCGACTACCCCGACGCGACGCTG
CTCGACGCGCTCGATTCGAT

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVESKPGVGYPNEWENQRRWHGGWQRHADGRLTPRGGN
RLRVLAKLFANPDLPQIDDYYEPFTFDYAHLHDAGPTGAPPVARPRSLVSGRRLDKIVGGPNWEEILGGEFDKRARDANF
EAVQKEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
EKCIFCYPRIEAGEPTVCSETCVGRIRYLGVLLYDADRIGEAAAVERETDLYEAQCSMFLDPHDPLVIAQAERDGVPAAW
LEAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANAGALGMHGLLPDVGALRIPLRYLANLLTAGDEAP
VKLALERLLAMRGFMRSRHVDGASDAEARAVLDQAGLTVAQVEEMYRYLAIANHEDRFVIPSSHREYAEDTFDLRAACGF
SFGNGCSDGRSDTGLFGSARPRKTIPIREVTP

Sequences:

>Translated_512_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVESKPGVGYPNEWENQRRWHGGWQRHADGRLTPRGGN
RLRVLAKLFANPDLPQIDDYYEPFTFDYAHLHDAGPTGAPPVARPRSLVSGRRLDKIVGGPNWEEILGGEFDKRARDANF
EAVQKEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
EKCIFCYPRIEAGEPTVCSETCVGRIRYLGVLLYDADRIGEAAAVERETDLYEAQCSMFLDPHDPLVIAQAERDGVPAAW
LEAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANAGALGMHGLLPDVGALRIPLRYLANLLTAGDEAP
VKLALERLLAMRGFMRSRHVDGASDAEARAVLDQAGLTVAQVEEMYRYLAIANHEDRFVIPSSHREYAEDTFDLRAACGF
SFGNGCSDGRSDTGLFGSARPRKTIPIREVTP
>Mature_512_residues
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVESKPGVGYPNEWENQRRWHGGWQRHADGRLTPRGGN
RLRVLAKLFANPDLPQIDDYYEPFTFDYAHLHDAGPTGAPPVARPRSLVSGRRLDKIVGGPNWEEILGGEFDKRARDANF
EAVQKEIYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
EKCIFCYPRIEAGEPTVCSETCVGRIRYLGVLLYDADRIGEAAAVERETDLYEAQCSMFLDPHDPLVIAQAERDGVPAAW
LEAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPINAAANAGALGMHGLLPDVGALRIPLRYLANLLTAGDEAP
VKLALERLLAMRGFMRSRHVDGASDAEARAVLDQAGLTVAQVEEMYRYLAIANHEDRFVIPSSHREYAEDTFDLRAACGF
SFGNGCSDGRSDTGLFGSARPRKTIPIREVTP

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=501, Percent_Identity=71.2574850299401, Blast_Score=783, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=502, Percent_Identity=70.1195219123506, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=114, Percent_Identity=41.2280701754386, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787122, Length=114, Percent_Identity=41.2280701754386, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI2367345, Length=146, Percent_Identity=25.3424657534247, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=36.3636363636364, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57392; Mature: 57392

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVESKPGVGYPNEWENQR
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCH
RWHGGWQRHADGRLTPRGGNRLRVLAKLFANPDLPQIDDYYEPFTFDYAHLHDAGPTGAP
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PVARPRSLVSGRRLDKIVGGPNWEEILGGEFDKRARDANFEAVQKEIYGQFENTFMMYLP
CCCCCHHHHCCHHHHHHHCCCCHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECCCCC
EKCIFCYPRIEAGEPTVCSETCVGRIRYLGVLLYDADRIGEAAAVERETDLYEAQCSMFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECHHHCCCHHHHHHHHHHHHHHHHEEE
DPHDPLVIAQAERDGVPAAWLEAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCH
INAAANAGALGMHGLLPDVGALRIPLRYLANLLTAGDEAPVKLALERLLAMRGFMRSRHV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DGASDAEARAVLDQAGLTVAQVEEMYRYLAIANHEDRFVIPSSHREYAEDTFDLRAACGF
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCC
SFGNGCSDGRSDTGLFGSARPRKTIPIREVTP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVESKPGVGYPNEWENQR
CCCCEEEEEEEEHHHHHCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCH
RWHGGWQRHADGRLTPRGGNRLRVLAKLFANPDLPQIDDYYEPFTFDYAHLHDAGPTGAP
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PVARPRSLVSGRRLDKIVGGPNWEEILGGEFDKRARDANFEAVQKEIYGQFENTFMMYLP
CCCCCHHHHCCHHHHHHHCCCCHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREEDGVVLIDQDKCRGWRMCVSGCPYKKIYYNWKSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECCCCC
EKCIFCYPRIEAGEPTVCSETCVGRIRYLGVLLYDADRIGEAAAVERETDLYEAQCSMFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEECHHHCCCHHHHHHHHHHHHHHHHEEE
DPHDPLVIAQAERDGVPAAWLEAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCEEEEECCCCH
INAAANAGALGMHGLLPDVGALRIPLRYLANLLTAGDEAPVKLALERLLAMRGFMRSRHV
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DGASDAEARAVLDQAGLTVAQVEEMYRYLAIANHEDRFVIPSSHREYAEDTFDLRAACGF
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCC
SFGNGCSDGRSDTGLFGSARPRKTIPIREVTP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]