Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 238024761

GI number: 238024761

Start: 1790994

End: 1791866

Strand: Reverse

Name: gcvA [H]

Synonym: bglu_2g13990

Alternate gene names: 238024761

Gene position: 1791866-1790994 (Counterclockwise)

Preceding gene: 238024762

Following gene: 238024754

Centisome position: 63.38

GC content: 72.85

Gene sequence:

>873_bases
ATGCAACGTCCGCCGCCTCTCAATGCCCTGCACGTGTTCGTCACGGCCGCGCGCCACCTGAGCTTCACGCGCGCGGCCGC
CGCGCTGTGCCTGACGCAGGGCGCGGTCAGCCGGCAAATCCAGTCGCTCGAGGCGCACTACGGCTTTTTGCTGTTCGTGC
GGCGCGCGAAGGGGCTCGCGCTGACGCCGGAGGGCGAGCAACTGCTGCCCGTGGTGGCCGACAGCCTCGCGCGCATCGAC
GAGGTGTCGCGGCGCCTCACGCGCGAGCGCAGCGATCTCGCGCTGAAGGTGCCCACCTGCGTGATGCGCTGGATCCTGCC
GAAAGTCATGCAGTTCCAGGCCGAGCACCCCGACCTGCACGTGCAGTTGACCACCGCCTGGCGGCACGACGTCGATTTCG
CGACCGAGCCGTTCGACGCGGCGATCGTCTACGGCGACGCGGGCGGCCCGGGCATCCACGCGGTGCCCTTGTTCGACGAG
CGGCTCACGCCCGTGTGCGCGCCAGAGCTGCTGGCGGCCCGGCCGCTCGCCGCGGGCGCCGATCTGGCGCGCCACACGCT
GCTGCACCCCACGCGCGACCATCGCGACTGGCGGCAGTGGCTCGCCCACGCCGGCATCGACGGCATCGATGCGGCGCGCG
GGCCGAGCTTCGACACGCTCGACCTGGCGACCAACGCGGCCATGCAGGGCTTCGGCGTGGCCATCGGCGACCTCACGCTG
GTGGCCGAGGACGTGGCAGCACGGCGCCTGGCACGGCCGTTCGACGCGGTGCTGGCCACCGGCGCGCGCTATGCGTTGGT
CTGCCCGGCGCGCAACGCCGCGCAGCCCAAGCTCGGCCTGTTCGGCGCCTGGCTCGCCGCGCATCGCGATTGA

Upstream 100 bases:

>100_bases
TTATCGTGATGCCTGACGCGCCGGCATTCGCATCCGCCATCGCCGGCGCGGCCGCTTGCCGTTCGTCAACCCGCTCGCCC
GCGCGTCGCGCGCCTTCGCC

Downstream 100 bases:

>100_bases
GCGGCCCGGCCGGCCCGGGCTCAGGTGCTGCGCTTGCGCACCGCGCCGACGACCACCAGCAGCACGATCGCGCCGATGAT
CGAGGCGATCCAGCCCGCCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MQRPPPLNALHVFVTAARHLSFTRAAAALCLTQGAVSRQIQSLEAHYGFLLFVRRAKGLALTPEGEQLLPVVADSLARID
EVSRRLTRERSDLALKVPTCVMRWILPKVMQFQAEHPDLHVQLTTAWRHDVDFATEPFDAAIVYGDAGGPGIHAVPLFDE
RLTPVCAPELLAARPLAAGADLARHTLLHPTRDHRDWRQWLAHAGIDGIDAARGPSFDTLDLATNAAMQGFGVAIGDLTL
VAEDVAARRLARPFDAVLATGARYALVCPARNAAQPKLGLFGAWLAAHRD

Sequences:

>Translated_290_residues
MQRPPPLNALHVFVTAARHLSFTRAAAALCLTQGAVSRQIQSLEAHYGFLLFVRRAKGLALTPEGEQLLPVVADSLARID
EVSRRLTRERSDLALKVPTCVMRWILPKVMQFQAEHPDLHVQLTTAWRHDVDFATEPFDAAIVYGDAGGPGIHAVPLFDE
RLTPVCAPELLAARPLAAGADLARHTLLHPTRDHRDWRQWLAHAGIDGIDAARGPSFDTLDLATNAAMQGFGVAIGDLTL
VAEDVAARRLARPFDAVLATGARYALVCPARNAAQPKLGLFGAWLAAHRD
>Mature_290_residues
MQRPPPLNALHVFVTAARHLSFTRAAAALCLTQGAVSRQIQSLEAHYGFLLFVRRAKGLALTPEGEQLLPVVADSLARID
EVSRRLTRERSDLALKVPTCVMRWILPKVMQFQAEHPDLHVQLTTAWRHDVDFATEPFDAAIVYGDAGGPGIHAVPLFDE
RLTPVCAPELLAARPLAAGADLARHTLLHPTRDHRDWRQWLAHAGIDGIDAARGPSFDTLDLATNAAMQGFGVAIGDLTL
VAEDVAARRLARPFDAVLATGARYALVCPARNAAQPKLGLFGAWLAAHRD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=37.9310344827586, Blast_Score=191, Evalue=3e-50,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=29.0657439446367, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=29.7202797202797, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=26.6211604095563, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790262, Length=214, Percent_Identity=31.7757009345794, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787589, Length=284, Percent_Identity=27.8169014084507, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI157672245, Length=166, Percent_Identity=30.1204819277108, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=23.2638888888889, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31548; Mature: 31548

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRPPPLNALHVFVTAARHLSFTRAAAALCLTQGAVSRQIQSLEAHYGFLLFVRRAKGLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCE
LTPEGEQLLPVVADSLARIDEVSRRLTRERSDLALKVPTCVMRWILPKVMQFQAEHPDLH
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCCCEE
VQLTTAWRHDVDFATEPFDAAIVYGDAGGPGIHAVPLFDERLTPVCAPELLAARPLAAGA
EEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCHHCCCCCCCHHHHHCCCCCCCH
DLARHTLLHPTRDHRDWRQWLAHAGIDGIDAARGPSFDTLDLATNAAMQGFGVAIGDLTL
HHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEHHHHHH
VAEDVAARRLARPFDAVLATGARYALVCPARNAAQPKLGLFGAWLAAHRD
HHHHHHHHHHHCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQRPPPLNALHVFVTAARHLSFTRAAAALCLTQGAVSRQIQSLEAHYGFLLFVRRAKGLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCE
LTPEGEQLLPVVADSLARIDEVSRRLTRERSDLALKVPTCVMRWILPKVMQFQAEHPDLH
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCCCEE
VQLTTAWRHDVDFATEPFDAAIVYGDAGGPGIHAVPLFDERLTPVCAPELLAARPLAAGA
EEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCHHCCCCCCCHHHHHCCCCCCCH
DLARHTLLHPTRDHRDWRQWLAHAGIDGIDAARGPSFDTLDLATNAAMQGFGVAIGDLTL
HHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEHHHHHH
VAEDVAARRLARPFDAVLATGARYALVCPARNAAQPKLGLFGAWLAAHRD
HHHHHHHHHHHCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]