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The map label for this gene is paaF [C]

Identifier: 238024456

GI number: 238024456

Start: 1343686

End: 1344513

Strand: Reverse

Name: paaF [C]

Synonym: bglu_2g10520

Alternate gene names: 238024456

Gene position: 1344513-1343686 (Counterclockwise)

Preceding gene: 238024458

Following gene: 238024455

Centisome position: 47.55

GC content: 64.86

Gene sequence:

>828_bases
ATGAGCTACGAAGGTCGCTGGACAACGGTCAAGGTCACGGTCGAGGCGGGCATCGGCTGGGTGGTGCTGAATCGCCCCGA
GAAGCGCAACGCGATGAGCCCGACGCTGAACAAGGAAATGATCGACGTGCTCGAAACGCTCGAGCTCGACGACGAGGCGC
AGGTGCTGGTACTGACCGGCGAAGGCGATGCGTGGACCGCCGGCATGGACCTGAAGGAATATTTCCGCGAGGTGGACGCG
GCCTCGGACGTGGTGCAGGAGCGCATTCGCCGCGACGCGAGCCGCTGGCAATGGCAACTGCTGCGCATGTATTCGAAGCC
GACCATCGCGATGGTCAACGGCTGGTGTTTCGGCGGCGGGTTTTCGCCGCTCGTCGCCTGCGATCTCGCCATCGCCGCCG
ACGAGGCCACCTTCGGCCTGTCCGAAATCAACTGGGGCATTCCCCCCGGCAACCTGGTGAGCAAGGCCATGGCGGACACC
GTCGGCCATCGCCAGGCGCTCTACTACATCATGACCGGCGACACCTTCACCGGCAAGCAGGCCGCGCAGATGGGCCTGGT
CAACCAGAGCGTGCCGCGCGCGGCCTTGCGCGAGGCGACCGTCGCGCTCGCGGCCAAGCTGCTCGACAAGAACCCGGTGG
TGCTGCGCAACGCGAAGCACGGCTTCAAGCGCAGCCGCGAACTGACCTGGGAGCAGAACGAGGACTACCTGTACGCCAAG
CTCGACCAGGCCAATTATCGCGACAAGGAAGGCGGCCGCGAAAAGGGCCTCAAGCAGTTTCTCGACGACAAGAGCATCAA
GCCGGGCCTGCAAGCCTACAAGCGCTGA

Upstream 100 bases:

>100_bases
GGTTTTTACCAGCACGGCGATATTCCGCTTTACTTATCAGGCAGCCTGATATTAAATTGTCGTCAGGCCGCAGTCGCGGC
ACCATTCACGGAGAAATTCA

Downstream 100 bases:

>100_bases
TACGGCCGGCTCGAGCGGGCACACGGAGGAGACAACACCATGAACGAGATCGGTCTGTTGATCGACGGTCAGGCACGGCC
GGCGTCGAACGGCGGCACCT

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSYEGRWTTVKVTVEAGIGWVVLNRPEKRNAMSPTLNKEMIDVLETLELDDEAQVLVLTGEGDAWTAGMDLKEYFREVDA
ASDVVQERIRRDASRWQWQLLRMYSKPTIAMVNGWCFGGGFSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADT
VGHRQALYYIMTGDTFTGKQAAQMGLVNQSVPRAALREATVALAAKLLDKNPVVLRNAKHGFKRSRELTWEQNEDYLYAK
LDQANYRDKEGGREKGLKQFLDDKSIKPGLQAYKR

Sequences:

>Translated_275_residues
MSYEGRWTTVKVTVEAGIGWVVLNRPEKRNAMSPTLNKEMIDVLETLELDDEAQVLVLTGEGDAWTAGMDLKEYFREVDA
ASDVVQERIRRDASRWQWQLLRMYSKPTIAMVNGWCFGGGFSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADT
VGHRQALYYIMTGDTFTGKQAAQMGLVNQSVPRAALREATVALAAKLLDKNPVVLRNAKHGFKRSRELTWEQNEDYLYAK
LDQANYRDKEGGREKGLKQFLDDKSIKPGLQAYKR
>Mature_274_residues
SYEGRWTTVKVTVEAGIGWVVLNRPEKRNAMSPTLNKEMIDVLETLELDDEAQVLVLTGEGDAWTAGMDLKEYFREVDAA
SDVVQERIRRDASRWQWQLLRMYSKPTIAMVNGWCFGGGFSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADTV
GHRQALYYIMTGDTFTGKQAAQMGLVNQSVPRAALREATVALAAKLLDKNPVVLRNAKHGFKRSRELTWEQNEDYLYAKL
DQANYRDKEGGREKGLKQFLDDKSIKPGLQAYKR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=29.9595141700405, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=28, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=29.9610894941634, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI45643119, Length=224, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI260275230, Length=224, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI260274832, Length=229, Percent_Identity=28.82096069869, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI20127408, Length=190, Percent_Identity=28.4210526315789, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI25453481, Length=224, Percent_Identity=23.2142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI51538573, Length=224, Percent_Identity=23.2142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4757966, Length=224, Percent_Identity=23.2142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI51538581, Length=224, Percent_Identity=23.2142857142857, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4757968, Length=224, Percent_Identity=23.6607142857143, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI49355787, Length=224, Percent_Identity=23.6607142857143, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221307494, Length=229, Percent_Identity=24.0174672489083, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136756, Length=229, Percent_Identity=24.0174672489083, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=229, Percent_Identity=24.0174672489083, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=31.7269076305221, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788597, Length=207, Percent_Identity=37.1980676328502, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI221142681, Length=230, Percent_Identity=27.3913043478261, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788682, Length=195, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082183, Length=216, Percent_Identity=24.0740740740741, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790281, Length=208, Percent_Identity=27.4038461538462, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787660, Length=197, Percent_Identity=28.4263959390863, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=29.4573643410853, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=28, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=206, Percent_Identity=28.6407766990291, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=224, Percent_Identity=27.2321428571429, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=24.2424242424242, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=29.7560975609756, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=205, Percent_Identity=27.3170731707317, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=222, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=167, Percent_Identity=25.748502994012, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20129971, Length=273, Percent_Identity=27.4725274725275, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24653477, Length=273, Percent_Identity=27.4725274725275, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24650670, Length=226, Percent_Identity=29.646017699115, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357171, Length=184, Percent_Identity=27.7173913043478, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24653139, Length=234, Percent_Identity=29.0598290598291, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920382, Length=244, Percent_Identity=25.8196721311475, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922422, Length=199, Percent_Identity=26.6331658291457, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583165, Length=279, Percent_Identity=23.2974910394265, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30825; Mature: 30694

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEGRWTTVKVTVEAGIGWVVLNRPEKRNAMSPTLNKEMIDVLETLELDDEAQVLVLTG
CCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
EGDAWTAGMDLKEYFREVDAASDVVQERIRRDASRWQWQLLRMYSKPTIAMVNGWCFGGG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEECCC
FSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADTVGHRQALYYIMTGDTFTGKQ
CCHHHHHHHHEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
AAQMGLVNQSVPRAALREATVALAAKLLDKNPVVLRNAKHGFKRSRELTWEQNEDYLYAK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCCEEEEE
LDQANYRDKEGGREKGLKQFLDDKSIKPGLQAYKR
ECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCC
>Mature Secondary Structure 
SYEGRWTTVKVTVEAGIGWVVLNRPEKRNAMSPTLNKEMIDVLETLELDDEAQVLVLTG
CCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
EGDAWTAGMDLKEYFREVDAASDVVQERIRRDASRWQWQLLRMYSKPTIAMVNGWCFGGG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECEEECCC
FSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMADTVGHRQALYYIMTGDTFTGKQ
CCHHHHHHHHEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
AAQMGLVNQSVPRAALREATVALAAKLLDKNPVVLRNAKHGFKRSRELTWEQNEDYLYAK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCCEEEEE
LDQANYRDKEGGREKGLKQFLDDKSIKPGLQAYKR
ECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA