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The map label for this gene is hipO [H]

Identifier: 238024450

GI number: 238024450

Start: 1335485

End: 1336723

Strand: Direct

Name: hipO [H]

Synonym: bglu_2g10460

Alternate gene names: 238024450

Gene position: 1335485-1336723 (Clockwise)

Preceding gene: 238024449

Following gene: 238024457

Centisome position: 47.23

GC content: 69.49

Gene sequence:

>1239_bases
ATGCGCCGGCAGGGCAGCAGGCAAGCCGTTTATTTTTCGAAGGTGCCGAAATTGGAACACGCCGTCATTTCCGACACGTC
GCTGAGCACGCATCAGGCACACTGGGCCGGATTGCGCCGCGATCTGCACGCGCATCCCGAGCTGCGGTTCGAGGAGCACC
GCACCGCCGACGTCGTCGCCCGCGAACTGGAGTCGCTCGGCTATTCGGTGGCGCGCGGGCTGGGCGGCACCGGTGTGGTG
GCCAGCCTGGCCGGCACCGACCCGGCACGCGGCATCGTGCTGCGCGCCGATCTGGACGCGCTGCCGATCCACGAGGCCAA
CGATTTCGCGCATGCCTCGTGCGCGCACGGGATCATGCATGCCTGCGGCCATGACGGCCATACGGTCATGCTGCTGGGCG
CCGCGCGCATGCTGAAGTCGCTGCCGCCGCTGCCGGGCAGCGTGCATTTCGTGTTTCAGCCGGGCGAGGAGGGCGGCGCG
GGCGCGCGCAAGATGATCGACGACGGGCTGTTCGAGCAATATCCCACCGAAGCGGTGTTCGGCATGCACAACTGGCCCGG
CTTGCCGGCCGGGCAGTTCGGGCTGCGCACCGGGCCCATCATGGCCGCGGGCTCGCGCTTCCGGATCACGGTGCGGGGCA
AGGGCGCGCACGCGGCGCAGCCGCATCTGGGCATCGATCCGGTGCCGCTCGCCTGCCTGATGGTGCTGCAGTGCCAGACC
ATCGCCGCACGGCACAAGGACCCCGTCCAACCCGCCGTGATTTCCGTCTGCATGTTCCAGGCGGGCACGACCGACAACGT
CATTCCAGACACGGCCGAGCTGCGCGGCACGATTCGCACCCTGTCGTCCGAGCTGCAGCAGAGGCTGCAGCGCGACGTCC
GGCAGGCCTGCGAGGGGCTGGCCGCGGCGCAGGGCGCGCAGGTCGAGGTGGAGTTCTTTCAATACTATCCGGCGACGGTC
AACACGCCGGCCGAGACGGCGCTGTGCGAAGCGGTGATCCGCGACACGTTCGGCGAGGCACGCCTGCACCGCGAGGTGCC
GCCGAACATGACGTCCGAGGACTTCGGCTTCATGCTGGAGGCGCGGCCGGGCGCCTACGTGCTGATCGGCAATGCGCCGG
ACGGCGCGGCCGCGGCGGCGCTGCATCACCCGAAATACGATTTCAACGACGCCATCATTCCGGCCGGCGTCCGGTACTGG
GTGGCCCTGGCGCAGCGTTACTTCGGCCGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GTCGGGCAACGGCCGCGAGTACGGGATCGAAGGGATGGAGGAGTACCTGGAGCTCAAGTCGGTGCTGGGCTACTACGACT
GACGCGGCCGCGGCGCGCCG

Downstream 100 bases:

>100_bases
GGCGCCGGGGCGCCGCGACCGGCCGGCCAGGCCGCATGGGGTGACGGCCGGCGGCGGCGGTGCCTTGGCGGCCGCCTCAG
GCCGTCACGCCCTCGATGCC

Product: metal-dependent amidase/aminoacylase/carboxypeptidase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MRRQGSRQAVYFSKVPKLEHAVISDTSLSTHQAHWAGLRRDLHAHPELRFEEHRTADVVARELESLGYSVARGLGGTGVV
ASLAGTDPARGIVLRADLDALPIHEANDFAHASCAHGIMHACGHDGHTVMLLGAARMLKSLPPLPGSVHFVFQPGEEGGA
GARKMIDDGLFEQYPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGSRFRITVRGKGAHAAQPHLGIDPVPLACLMVLQCQT
IAARHKDPVQPAVISVCMFQAGTTDNVIPDTAELRGTIRTLSSELQQRLQRDVRQACEGLAAAQGAQVEVEFFQYYPATV
NTPAETALCEAVIRDTFGEARLHREVPPNMTSEDFGFMLEARPGAYVLIGNAPDGAAAAALHHPKYDFNDAIIPAGVRYW
VALAQRYFGRAA

Sequences:

>Translated_412_residues
MRRQGSRQAVYFSKVPKLEHAVISDTSLSTHQAHWAGLRRDLHAHPELRFEEHRTADVVARELESLGYSVARGLGGTGVV
ASLAGTDPARGIVLRADLDALPIHEANDFAHASCAHGIMHACGHDGHTVMLLGAARMLKSLPPLPGSVHFVFQPGEEGGA
GARKMIDDGLFEQYPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGSRFRITVRGKGAHAAQPHLGIDPVPLACLMVLQCQT
IAARHKDPVQPAVISVCMFQAGTTDNVIPDTAELRGTIRTLSSELQQRLQRDVRQACEGLAAAQGAQVEVEFFQYYPATV
NTPAETALCEAVIRDTFGEARLHREVPPNMTSEDFGFMLEARPGAYVLIGNAPDGAAAAALHHPKYDFNDAIIPAGVRYW
VALAQRYFGRAA
>Mature_412_residues
MRRQGSRQAVYFSKVPKLEHAVISDTSLSTHQAHWAGLRRDLHAHPELRFEEHRTADVVARELESLGYSVARGLGGTGVV
ASLAGTDPARGIVLRADLDALPIHEANDFAHASCAHGIMHACGHDGHTVMLLGAARMLKSLPPLPGSVHFVFQPGEEGGA
GARKMIDDGLFEQYPTEAVFGMHNWPGLPAGQFGLRTGPIMAAGSRFRITVRGKGAHAAQPHLGIDPVPLACLMVLQCQT
IAARHKDPVQPAVISVCMFQAGTTDNVIPDTAELRGTIRTLSSELQQRLQRDVRQACEGLAAAQGAQVEVEFFQYYPATV
NTPAETALCEAVIRDTFGEARLHREVPPNMTSEDFGFMLEARPGAYVLIGNAPDGAAAAALHHPKYDFNDAIIPAGVRYW
VALAQRYFGRAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=427, Percent_Identity=30.2107728337237, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787598, Length=360, Percent_Identity=28.0555555555556, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 44419; Mature: 44419

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRQGSRQAVYFSKVPKLEHAVISDTSLSTHQAHWAGLRRDLHAHPELRFEEHRTADVVA
CCCCCCCCEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHH
RELESLGYSVARGLGGTGVVASLAGTDPARGIVLRADLDALPIHEANDFAHASCAHGIMH
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
ACGHDGHTVMLLGAARMLKSLPPLPGSVHFVFQPGEEGGAGARKMIDDGLFEQYPTEAVF
HHCCCCCEEEEEEHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHC
GMHNWPGLPAGQFGLRTGPIMAAGSRFRITVRGKGAHAAQPHLGIDPVPLACLMVLQCQT
CCCCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
IAARHKDPVQPAVISVCMFQAGTTDNVIPDTAELRGTIRTLSSELQQRLQRDVRQACEGL
HHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAQGAQVEVEFFQYYPATVNTPAETALCEAVIRDTFGEARLHREVPPNMTSEDFGFMLE
HHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
ARPGAYVLIGNAPDGAAAAALHHPKYDFNDAIIPAGVRYWVALAQRYFGRAA
ECCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRQGSRQAVYFSKVPKLEHAVISDTSLSTHQAHWAGLRRDLHAHPELRFEEHRTADVVA
CCCCCCCCEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHH
RELESLGYSVARGLGGTGVVASLAGTDPARGIVLRADLDALPIHEANDFAHASCAHGIMH
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
ACGHDGHTVMLLGAARMLKSLPPLPGSVHFVFQPGEEGGAGARKMIDDGLFEQYPTEAVF
HHCCCCCEEEEEEHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHC
GMHNWPGLPAGQFGLRTGPIMAAGSRFRITVRGKGAHAAQPHLGIDPVPLACLMVLQCQT
CCCCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
IAARHKDPVQPAVISVCMFQAGTTDNVIPDTAELRGTIRTLSSELQQRLQRDVRQACEGL
HHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAQGAQVEVEFFQYYPATVNTPAETALCEAVIRDTFGEARLHREVPPNMTSEDFGFMLE
HHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
ARPGAYVLIGNAPDGAAAAALHHPKYDFNDAIIPAGVRYWVALAQRYFGRAA
ECCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]