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The map label for this gene is artJ [H]

Identifier: 238024354

GI number: 238024354

Start: 1166939

End: 1167745

Strand: Direct

Name: artJ [H]

Synonym: bglu_2g09470

Alternate gene names: 238024354

Gene position: 1166939-1167745 (Clockwise)

Preceding gene: 238024353

Following gene: 238024355

Centisome position: 41.27

GC content: 59.11

Gene sequence:

>807_bases
ATGTTCCAACCTACGCCGCACCGTGCTTCGGGCAAGCACGCCAGTTTCTTCCAGCGCATTCTCGTCACCGCTGCCTTGGC
GGTCGCCGCCGCGTTGGGAGCCAGCCCGGCATGGGCAGCGCAACTGACGGTTTCCGCCGGAACCACGCCGTCCAGCATTC
CCAACGCCTTCTTGAACATCAAGACCAATCAGATCGAAGGCGTCATGCCGGACGTCATCCGCGCAATCGGGCAGCGTGAG
GGGTTCGGCGTTTCATTCAACGTCATTCCCTACTCCGCCTTGATCCAGTCGGTCGTCTCCGACAAGATCGACCTGATCGT
GGCCGGAATGACGCCGACGTCGAGGCGGGAAGAGGTGATCGATTTTTCTCAGCCCGTGGCTTTCTTTGGAAAGGGCATCA
TCGTCAGCGCCAACGACAAGCATGCTTACCGGACGATTGACGACCTGAAGGGCACGGTGGTCGGCGCCGGCGCCGGCGAG
GAAACCTCGGAGGAACTCGCGAAGCTCGGCATCTTCAAGGAAGTGAAGTTGTACAACAATGCCGCCGATATGGCCCGCGA
TGTCACGCTTGGCCGGATTACGGCAGCATTCAATGATTATCCGATCTGGAAGGCGCAGGAGGCGGCCGGTACGCTGCAGG
GCTGCCGTGTGGTGGACGGATTCAAACCGCTCCGGCAGGTCCCGATTGCATTCGGCGTGAAGAAAGGCAATACGGCCTTG
CTGTCGAAAATCAACGATGGCTTGGCAAAGATCAAGGCGGACGGGACGTTGGACAAGATCCTGAAGAAATGGAATTACGT
CGATTGA

Upstream 100 bases:

>100_bases
GGCACCGCATTGAATGCGTTTCGGCATGTAAAGGCAGGCGAACCTCTTGTCGGCATGCTTTTGCAGCACGTGGATAAAGA
AACTTCCTCAAGGATAGACC

Downstream 100 bases:

>100_bases
CGTGCCGGATGTCAAGCGGGCCTGGTGCGAGGGCATAGCGGCGCGGGTCTTTTGCCGGGTCGAGCGGGACGCGCTCCGAC
TCGCCGGATGCACCGGGGTA

Product: ABC polar amino acid family transporter periplasmic ligand binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MFQPTPHRASGKHASFFQRILVTAALAVAAALGASPAWAAQLTVSAGTTPSSIPNAFLNIKTNQIEGVMPDVIRAIGQRE
GFGVSFNVIPYSALIQSVVSDKIDLIVAGMTPTSRREEVIDFSQPVAFFGKGIIVSANDKHAYRTIDDLKGTVVGAGAGE
ETSEELAKLGIFKEVKLYNNAADMARDVTLGRITAAFNDYPIWKAQEAAGTLQGCRVVDGFKPLRQVPIAFGVKKGNTAL
LSKINDGLAKIKADGTLDKILKKWNYVD

Sequences:

>Translated_268_residues
MFQPTPHRASGKHASFFQRILVTAALAVAAALGASPAWAAQLTVSAGTTPSSIPNAFLNIKTNQIEGVMPDVIRAIGQRE
GFGVSFNVIPYSALIQSVVSDKIDLIVAGMTPTSRREEVIDFSQPVAFFGKGIIVSANDKHAYRTIDDLKGTVVGAGAGE
ETSEELAKLGIFKEVKLYNNAADMARDVTLGRITAAFNDYPIWKAQEAAGTLQGCRVVDGFKPLRQVPIAFGVKKGNTAL
LSKINDGLAKIKADGTLDKILKKWNYVD
>Mature_268_residues
MFQPTPHRASGKHASFFQRILVTAALAVAAALGASPAWAAQLTVSAGTTPSSIPNAFLNIKTNQIEGVMPDVIRAIGQRE
GFGVSFNVIPYSALIQSVVSDKIDLIVAGMTPTSRREEVIDFSQPVAFFGKGIIVSANDKHAYRTIDDLKGTVVGAGAGE
ETSEELAKLGIFKEVKLYNNAADMARDVTLGRITAAFNDYPIWKAQEAAGTLQGCRVVDGFKPLRQVPIAFGVKKGNTAL
LSKINDGLAKIKADGTLDKILKKWNYVD

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=255, Percent_Identity=30.1960784313725, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=26.984126984127, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788228, Length=201, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=28.3898305084746, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787031, Length=243, Percent_Identity=28.3950617283951, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28649; Mature: 28649

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQPTPHRASGKHASFFQRILVTAALAVAAALGASPAWAAQLTVSAGTTPSSIPNAFLNI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEEE
KTNQIEGVMPDVIRAIGQREGFGVSFNVIPYSALIQSVVSDKIDLIVAGMTPTSRREEVI
ECCCCCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
DFSQPVAFFGKGIIVSANDKHAYRTIDDLKGTVVGAGAGEETSEELAKLGIFKEVKLYNN
HCCCCHHHHCCCEEEECCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHCCHHHHHHHCC
AADMARDVTLGRITAAFNDYPIWKAQEAAGTLQGCRVVDGFKPLRQVPIAFGVKKGNTAL
HHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHCCEEHHCHHHHHHCCHHEEECCCCHHH
LSKINDGLAKIKADGTLDKILKKWNYVD
HHHHHHHHHEEECCCCHHHHHHHCCCCC
>Mature Secondary Structure
MFQPTPHRASGKHASFFQRILVTAALAVAAALGASPAWAAQLTVSAGTTPSSIPNAFLNI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCEEEE
KTNQIEGVMPDVIRAIGQREGFGVSFNVIPYSALIQSVVSDKIDLIVAGMTPTSRREEVI
ECCCCCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
DFSQPVAFFGKGIIVSANDKHAYRTIDDLKGTVVGAGAGEETSEELAKLGIFKEVKLYNN
HCCCCHHHHCCCEEEECCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHCCHHHHHHHCC
AADMARDVTLGRITAAFNDYPIWKAQEAAGTLQGCRVVDGFKPLRQVPIAFGVKKGNTAL
HHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHCCEEHHCHHHHHHCCHHEEECCCCHHH
LSKINDGLAKIKADGTLDKILKKWNYVD
HHHHHHHHHEEECCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]