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The map label for this gene is fdoI [H]

Identifier: 238024283

GI number: 238024283

Start: 1051867

End: 1052511

Strand: Direct

Name: fdoI [H]

Synonym: bglu_2g08660

Alternate gene names: 238024283

Gene position: 1051867-1052511 (Clockwise)

Preceding gene: 238024282

Following gene: 238024284

Centisome position: 37.2

GC content: 65.27

Gene sequence:

>645_bases
ATGAGCCACCAGGACCCGAACCTGATCGTCCGTTACTCCGCGAATGAACGCTCGAATCACTGGATCACCGCGATCTCGTT
CGTGCTGCTCGCGCTGTCCGGCCTCGCGCTGTTCCATCCGTCGATGTTCTGGCTCACCGCGCTGTTCGGCGGCGGGCAAT
GGACGCGCATCCTGCATCCGTTCGTCGGCGTCGTGATGTTCGTGTCGTTCGCGATCATGGTGGTGCGCTACTGGCATCAC
AATCTGCTCGACGCGGACGACCGCCGCTGGCTCGCACAGATCGGCGACGTGCTGGGCAATCGCGAGGAAAACCTGCCGCC
CGTCGGCCGCTACAACGCGGGGCAGAAGCTGCTGTTCTTCACGCTCGTCATCTGCCTGCTGCTGCTGCTGCTGTCGGGCA
TCGTGATCTGGCGCCGGTATTTCTCGCTGTATTTCCCGATCGAGGCCATCCGGCTCGCGGCCGTGGTGCATGCGGCCGCC
GCATTCGTGCTGATCGCCGGCATCATCGTCCACATCTACGCGGCGCTGTGGGTGAAGGGCTCGATCGGCGCGATGGTGCG
CGGCACGGTGTCGTTCGGCTGGGCGCGCAAGCACCATCCGAAGTGGTTCCGCCAGGCGGTGAAGCAGTCGGGGCGCGCGC
GCTAG

Upstream 100 bases:

>100_bases
CCGGGTTCTTCCACTACACGCGGGTCGGCCCCAACGAGGTGAGCGAGCAAGAGGACGCCGCCGCCCGTGAGGAAGCCCGC
CGGATCGAGGAGGACACCAA

Downstream 100 bases:

>100_bases
GGCGCGCCGCGCATCACGCGCCATCGCGCGCCGGCCGCCCGGCGCGCTTCGGGGCCATCCATGGGATGGCCCTTGTCGTT
TGGCGCGCGGCCGGCCGCGG

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSHQDPNLIVRYSANERSNHWITAISFVLLALSGLALFHPSMFWLTALFGGGQWTRILHPFVGVVMFVSFAIMVVRYWHH
NLLDADDRRWLAQIGDVLGNREENLPPVGRYNAGQKLLFFTLVICLLLLLLSGIVIWRRYFSLYFPIEAIRLAAVVHAAA
AFVLIAGIIVHIYAALWVKGSIGAMVRGTVSFGWARKHHPKWFRQAVKQSGRAR

Sequences:

>Translated_214_residues
MSHQDPNLIVRYSANERSNHWITAISFVLLALSGLALFHPSMFWLTALFGGGQWTRILHPFVGVVMFVSFAIMVVRYWHH
NLLDADDRRWLAQIGDVLGNREENLPPVGRYNAGQKLLFFTLVICLLLLLLSGIVIWRRYFSLYFPIEAIRLAAVVHAAA
AFVLIAGIIVHIYAALWVKGSIGAMVRGTVSFGWARKHHPKWFRQAVKQSGRAR
>Mature_213_residues
SHQDPNLIVRYSANERSNHWITAISFVLLALSGLALFHPSMFWLTALFGGGQWTRILHPFVGVVMFVSFAIMVVRYWHHN
LLDADDRRWLAQIGDVLGNREENLPPVGRYNAGQKLLFFTLVICLLLLLLSGIVIWRRYFSLYFPIEAIRLAAVVHAAAA
FVLIAGIIVHIYAALWVKGSIGAMVRGTVSFGWARKHHPKWFRQAVKQSGRAR

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=206, Percent_Identity=53.8834951456311, Blast_Score=220, Evalue=4e-59,
Organism=Escherichia coli, GI1787750, Length=208, Percent_Identity=45.1923076923077, Blast_Score=198, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24290; Mature: 24159

Theoretical pI: Translated: 11.22; Mature: 11.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQDPNLIVRYSANERSNHWITAISFVLLALSGLALFHPSMFWLTALFGGGQWTRILHP
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FVGVVMFVSFAIMVVRYWHHNLLDADDRRWLAQIGDVLGNREENLPPVGRYNAGQKLLFF
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
TLVICLLLLLLSGIVIWRRYFSLYFPIEAIRLAAVVHAAAAFVLIAGIIVHIYAALWVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIGAMVRGTVSFGWARKHHPKWFRQAVKQSGRAR
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SHQDPNLIVRYSANERSNHWITAISFVLLALSGLALFHPSMFWLTALFGGGQWTRILHP
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FVGVVMFVSFAIMVVRYWHHNLLDADDRRWLAQIGDVLGNREENLPPVGRYNAGQKLLFF
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
TLVICLLLLLLSGIVIWRRYFSLYFPIEAIRLAAVVHAAAAFVLIAGIIVHIYAALWVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIGAMVRGTVSFGWARKHHPKWFRQAVKQSGRAR
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]