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The map label for this gene is prpD [H]

Identifier: 238024236

GI number: 238024236

Start: 996631

End: 998082

Strand: Reverse

Name: prpD [H]

Synonym: bglu_2g08180

Alternate gene names: 238024236

Gene position: 998082-996631 (Counterclockwise)

Preceding gene: 238024237

Following gene: 238024235

Centisome position: 35.3

GC content: 69.9

Gene sequence:

>1452_bases
ATGTCCGCTCCGCAAACGAACGTCCGGCCCGCGCCGGACCCGGTACTGGTCGAGATCGCCGACTACGTGCTGGATGTCGA
GATCGGCAGTGCGCTCGCGCTCGACACGGCGCGCTACTGCCTGCTCGATACGCTCGGTTGCGGACTCGAGGCCCTGTCGT
ATCCGGCCTGCACCAAGCTGCTGGGGCCGCTGGTGCCGGGCACGCAGGTGCCGCATGGCGCCCGGGTGCCCGGCACCTCG
CTCGAACTCGACCCGGTCAAGGCCGCGTTCGACATCGGCACCACGATCCGCTGGCTCGACTTCAACGACACCTGGCTCGC
GGCCGAATGGGGCCATCCGTCCGACAACCTGGGCGGCATCCTGGCCACCGCCGACTGGCTCTCGCGCACCGCGGTGGCGG
CCGGCAAGCCGCCGCTCACGATGCGCGAGGTGCTGGTGGCGATGGTCAAGGCCCACGAGATCCAGGGCTGCCTCGCGCTC
GAGAACGCGTTCAACCGCGTCGGGCTCGACCACGTGCTGCTGGTCAAGGTCGCCTCCACCGCGGTGGTCGGCAAGCTGCT
GGGCCTCACGCGCGACGAGCTCGTCAACGCGCTGTCGCTCGCCTTCGTCGACGGCCAGGCGCTTCGCACCTACCGGCACG
CGCCCAACACCGGCTCGCGCAAGTCGTGGGCGGCCGGCGACGCCACCTCGCGCGCCGTGCGCCTCGCGCTGATCGCCCGG
ACCGGCGAGATGGGCTACCCCACGGCGCTCAGCGCGCCCGTCTGGGGCTTCTACGACGTGCTGTTCGAGGGCAAGCCGTT
CCGCTTTCAACGCCCCTACGGCAGCTACGTGATGGAGAACGTGCTGTTCAAGATCGCGTTCCCCGCCGAATTCCACGCAC
AGACCGCCGCCGAGGCGGCGCTCGTGCTGCATCGCCGGCTCGCCGAGGCGGGCCGCGGCGCCGCGTCGATCCGGCGCGTC
ACGATCCGCACGCACGAGGCCGCGCTCCGGATCATCGACAAGGCCGGGCCGCTCGCGAACCCCGCCGACCGCGACCACTG
CATCCAGTACATGGTTGCCGTGCCGCTGCTGTTCGGGCGCCTCACCGCCGCCGACTACGAGGACGACGTCGCGGCCGATC
CGCGCATCGACGCGCTGCGCGCCAAGATCGCCTGCGTCGAGGACCCGCAGTTCACGCGCGATTATCACGACCCGGACAAG
CGCGCGATCGCCAACGGATTGACCGTCGAGCTCGACGACGGCACCGTGTTGGACGAGGTGGTGGTCGAATATCCGCTCGG
CCACCGGCGCCGCCGCGCGCAGGGCATCCCGCTGCTGCTCGACAAATTCCGCAGCCACCTCGCGCGGCGCCTGCCGGCGA
AACAGCAACAAGCGATTCTCGACGTGTCGCTGGACCAGGCAAGGCTCGAGGCCATGCCGGTCAATGAATACGTCGACTTG
TATGTGATCTAG

Upstream 100 bases:

>100_bases
AACGAACTCGCCCCCAACACCGGCAAGTAAGCGCACCCGGCGCCACCGCCACCCAGCCAGCCATTCAGGAAGCAATCGGA
CCGCCACGACAGGAAACCCG

Downstream 100 bases:

>100_bases
CCGTCAGATACAGGACTTCTTTCCTCGCCTTAACCCAGGAAAAACACCATGGCCCACAATCTCCACAAGACACTGCAAGA
ATTCGACAGCGGCTCCGGCA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSAPQTNVRPAPDPVLVEIADYVLDVEIGSALALDTARYCLLDTLGCGLEALSYPACTKLLGPLVPGTQVPHGARVPGTS
LELDPVKAAFDIGTTIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVAAGKPPLTMREVLVAMVKAHEIQGCLAL
ENAFNRVGLDHVLLVKVASTAVVGKLLGLTRDELVNALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAR
TGEMGYPTALSAPVWGFYDVLFEGKPFRFQRPYGSYVMENVLFKIAFPAEFHAQTAAEAALVLHRRLAEAGRGAASIRRV
TIRTHEAALRIIDKAGPLANPADRDHCIQYMVAVPLLFGRLTAADYEDDVAADPRIDALRAKIACVEDPQFTRDYHDPDK
RAIANGLTVELDDGTVLDEVVVEYPLGHRRRRAQGIPLLLDKFRSHLARRLPAKQQQAILDVSLDQARLEAMPVNEYVDL
YVI

Sequences:

>Translated_483_residues
MSAPQTNVRPAPDPVLVEIADYVLDVEIGSALALDTARYCLLDTLGCGLEALSYPACTKLLGPLVPGTQVPHGARVPGTS
LELDPVKAAFDIGTTIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVAAGKPPLTMREVLVAMVKAHEIQGCLAL
ENAFNRVGLDHVLLVKVASTAVVGKLLGLTRDELVNALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAR
TGEMGYPTALSAPVWGFYDVLFEGKPFRFQRPYGSYVMENVLFKIAFPAEFHAQTAAEAALVLHRRLAEAGRGAASIRRV
TIRTHEAALRIIDKAGPLANPADRDHCIQYMVAVPLLFGRLTAADYEDDVAADPRIDALRAKIACVEDPQFTRDYHDPDK
RAIANGLTVELDDGTVLDEVVVEYPLGHRRRRAQGIPLLLDKFRSHLARRLPAKQQQAILDVSLDQARLEAMPVNEYVDL
YVI
>Mature_482_residues
SAPQTNVRPAPDPVLVEIADYVLDVEIGSALALDTARYCLLDTLGCGLEALSYPACTKLLGPLVPGTQVPHGARVPGTSL
ELDPVKAAFDIGTTIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTAVAAGKPPLTMREVLVAMVKAHEIQGCLALE
NAFNRVGLDHVLLVKVASTAVVGKLLGLTRDELVNALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIART
GEMGYPTALSAPVWGFYDVLFEGKPFRFQRPYGSYVMENVLFKIAFPAEFHAQTAAEAALVLHRRLAEAGRGAASIRRVT
IRTHEAALRIIDKAGPLANPADRDHCIQYMVAVPLLFGRLTAADYEDDVAADPRIDALRAKIACVEDPQFTRDYHDPDKR
AIANGLTVELDDGTVLDEVVVEYPLGHRRRRAQGIPLLLDKFRSHLARRLPAKQQQAILDVSLDQARLEAMPVNEYVDLY
VI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=483, Percent_Identity=76.1904761904762, Blast_Score=791, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=485, Percent_Identity=60.4123711340206, Blast_Score=607, Evalue=1e-174,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 52657; Mature: 52526

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPQTNVRPAPDPVLVEIADYVLDVEIGSALALDTARYCLLDTLGCGLEALSYPACTKL
CCCCCCCCCCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHH
LGPLVPGTQVPHGARVPGTSLELDPVKAAFDIGTTIRWLDFNDTWLAAEWGHPSDNLGGI
HHCCCCCCCCCCCCCCCCCCEECCCHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCH
LATADWLSRTAVAAGKPPLTMREVLVAMVKAHEIQGCLALENAFNRVGLDHVLLVKVAST
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AVVGKLLGLTRDELVNALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAR
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHEEEEEEEE
TGEMGYPTALSAPVWGFYDVLFEGKPFRFQRPYGSYVMENVLFKIAFPAEFHAQTAAEAA
CCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHEEEECCHHHHHHHHHHHH
LVLHRRLAEAGRGAASIRRVTIRTHEAALRIIDKAGPLANPADRDHCIQYMVAVPLLFGR
HHHHHHHHHHCCCHHHEEEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LTAADYEDDVAADPRIDALRAKIACVEDPQFTRDYHDPDKRAIANGLTVELDDGTVLDEV
HHCCCCCCCCCCCCCHHHHHHHEEEECCCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHH
VVEYPLGHRRRRAQGIPLLLDKFRSHLARRLPAKQQQAILDVSLDQARLEAMPVNEYVDL
HHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCCEEE
YVI
EEC
>Mature Secondary Structure 
SAPQTNVRPAPDPVLVEIADYVLDVEIGSALALDTARYCLLDTLGCGLEALSYPACTKL
CCCCCCCCCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHH
LGPLVPGTQVPHGARVPGTSLELDPVKAAFDIGTTIRWLDFNDTWLAAEWGHPSDNLGGI
HHCCCCCCCCCCCCCCCCCCEECCCHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCH
LATADWLSRTAVAAGKPPLTMREVLVAMVKAHEIQGCLALENAFNRVGLDHVLLVKVAST
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AVVGKLLGLTRDELVNALSLAFVDGQALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAR
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHEEEEEEEE
TGEMGYPTALSAPVWGFYDVLFEGKPFRFQRPYGSYVMENVLFKIAFPAEFHAQTAAEAA
CCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHEEEECCHHHHHHHHHHHH
LVLHRRLAEAGRGAASIRRVTIRTHEAALRIIDKAGPLANPADRDHCIQYMVAVPLLFGR
HHHHHHHHHHCCCHHHEEEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LTAADYEDDVAADPRIDALRAKIACVEDPQFTRDYHDPDKRAIANGLTVELDDGTVLDEV
HHCCCCCCCCCCCCCHHHHHHHEEEECCCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHH
VVEYPLGHRRRRAQGIPLLLDKFRSHLARRLPAKQQQAILDVSLDQARLEAMPVNEYVDL
HHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCCEEE
YVI
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]