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The map label for this gene is nylB' [H]

Identifier: 238024113

GI number: 238024113

Start: 813029

End: 814192

Strand: Direct

Name: nylB' [H]

Synonym: bglu_2g06860

Alternate gene names: 238024113

Gene position: 813029-814192 (Clockwise)

Preceding gene: 238024112

Following gene: 238024121

Centisome position: 28.76

GC content: 60.31

Gene sequence:

>1164_bases
ATGAGAATTAAAAAATCAGTTTCAATCGCGGCAATTGCGTCGCTGTGCCTGCTGATCGTTTCGTGGAACCTGTTGCGTCT
GCCGAGCCCGATCGTGCTCCTCGATCTCGAGTTCGGCTGGACCCCGACCAGCCAGACATTTCGGCTCTTTCAGAATCGAC
TGCTCGAAAAATCCGCCCAGCCCCTTGTGCTGAACACCGCCGAGCCTCGGTTCAATCACGCCGTGACCTGGAAAGGTGCC
GATTCGAACGTCGACGATTTCCTGAAGAACACGCAGACTCGCGCATTCCTGGTGATTCAGGACGGAAAGATCGCGTATGA
GAGATACGCGAACGGCTACAGCGCCGCCTCGAAATTTGCGTCTTACTCGGTAGCGAAGTCCTTTGTCGCCACGATGGTGG
GGGCTGCCTTGTTCGATGGACGGATTCACTCGCTGCAAGATCCGATCGGCAACTATCTCGAACCCGGCGACGTGCGCGAG
CAGTATCGTCGCGTGACCATCGAACAGCTGCTTTCCATGCGATCCGGCATCGACGCGGCCGAGAGCTACGATTCCGCCTT
CGCGCCGGTCGTGCAGATGTACCTGACCACCGATCTGAACCGCTTTCTGTCACGTGTATCGGGGTTTCGTCATCCGCCGG
GACAGGAATTCGAGTACCGGAGCGTGGACACGCTGGTGCTGTCGAAGGTCTTGACGCGCGCCACGGGCGTACCGCTGTCG
GCCTACGCGCAGCAGAAGCTGTGGACTCCGTTGGGCGCATCTTCCGACGCGAGCTGGAGTCTGGACAGCGCGACACACGG
CGTGGAGAAATCCTTCTGCTGCCTCAATGCCACCGCGCGCGATTTCGCGAAGCTCGGTTTGCTGTATCTGGCGGAAGGCG
ATCTGGCGGGCCACCGTATCGTGAGTCGTGACTGGGCGCTCGCGCCCGGCAAGTCTCCGAACCCGACCGATGCCCTTGCC
TACTTCGATGGCTGGTGGATTCCGCCGAACCACGGGCGCGACCGGGATTTTGCCGCAATCGGCATCTACGGGCAGTTCGT
CTACGTCAATCCGTCCACGCACACGACGATCGTGAAGCTCAGCGAGTACGGTGCGGAGCAGGACGAACTCGACACCTTGC
TGGCGATGCGCCAGATCGCGCACTCGATCACCGCCGCGCAATGA

Upstream 100 bases:

>100_bases
ATGCGGCAGTTGCCAGTTCCAACACGCCATCAATGTATAATTCGCTATCCTGCCAGCACCAAAAAGCCCTTGGCTATACA
AATAAAAAACCTCAAAAATA

Downstream 100 bases:

>100_bases
AATGCCGGTGAGTCGACCGCGCGGCCCGCTGCGCCGCGCGGTCGACGTGCATCAGCGAGTCCCGTCGATGCGATCCGAAA
ATTCAGGCTTGCCGGCGAAT

Product: beta-lactamase class C protein

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MRIKKSVSIAAIASLCLLIVSWNLLRLPSPIVLLDLEFGWTPTSQTFRLFQNRLLEKSAQPLVLNTAEPRFNHAVTWKGA
DSNVDDFLKNTQTRAFLVIQDGKIAYERYANGYSAASKFASYSVAKSFVATMVGAALFDGRIHSLQDPIGNYLEPGDVRE
QYRRVTIEQLLSMRSGIDAAESYDSAFAPVVQMYLTTDLNRFLSRVSGFRHPPGQEFEYRSVDTLVLSKVLTRATGVPLS
AYAQQKLWTPLGASSDASWSLDSATHGVEKSFCCLNATARDFAKLGLLYLAEGDLAGHRIVSRDWALAPGKSPNPTDALA
YFDGWWIPPNHGRDRDFAAIGIYGQFVYVNPSTHTTIVKLSEYGAEQDELDTLLAMRQIAHSITAAQ

Sequences:

>Translated_387_residues
MRIKKSVSIAAIASLCLLIVSWNLLRLPSPIVLLDLEFGWTPTSQTFRLFQNRLLEKSAQPLVLNTAEPRFNHAVTWKGA
DSNVDDFLKNTQTRAFLVIQDGKIAYERYANGYSAASKFASYSVAKSFVATMVGAALFDGRIHSLQDPIGNYLEPGDVRE
QYRRVTIEQLLSMRSGIDAAESYDSAFAPVVQMYLTTDLNRFLSRVSGFRHPPGQEFEYRSVDTLVLSKVLTRATGVPLS
AYAQQKLWTPLGASSDASWSLDSATHGVEKSFCCLNATARDFAKLGLLYLAEGDLAGHRIVSRDWALAPGKSPNPTDALA
YFDGWWIPPNHGRDRDFAAIGIYGQFVYVNPSTHTTIVKLSEYGAEQDELDTLLAMRQIAHSITAAQ
>Mature_387_residues
MRIKKSVSIAAIASLCLLIVSWNLLRLPSPIVLLDLEFGWTPTSQTFRLFQNRLLEKSAQPLVLNTAEPRFNHAVTWKGA
DSNVDDFLKNTQTRAFLVIQDGKIAYERYANGYSAASKFASYSVAKSFVATMVGAALFDGRIHSLQDPIGNYLEPGDVRE
QYRRVTIEQLLSMRSGIDAAESYDSAFAPVVQMYLTTDLNRFLSRVSGFRHPPGQEFEYRSVDTLVLSKVLTRATGVPLS
AYAQQKLWTPLGASSDASWSLDSATHGVEKSFCCLNATARDFAKLGLLYLAEGDLAGHRIVSRDWALAPGKSPNPTDALA
YFDGWWIPPNHGRDRDFAAIGIYGQFVYVNPSTHTTIVKLSEYGAEQDELDTLLAMRQIAHSITAAQ

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 42856; Mature: 42856

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKKSVSIAAIASLCLLIVSWNLLRLPSPIVLLDLEFGWTPTSQTFRLFQNRLLEKSAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
PLVLNTAEPRFNHAVTWKGADSNVDDFLKNTQTRAFLVIQDGKIAYERYANGYSAASKFA
CEEEECCCCCCCCEEEECCCCCCHHHHHHCCCCEEEEEEECCCEEHHHHCCCHHHHHHHH
SYSVAKSFVATMVGAALFDGRIHSLQDPIGNYLEPGDVREQYRRVTIEQLLSMRSGIDAA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
ESYDSAFAPVVQMYLTTDLNRFLSRVSGFRHPPGQEFEYRSVDTLVLSKVLTRATGVPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
AYAQQKLWTPLGASSDASWSLDSATHGVEKSFCCLNATARDFAKLGLLYLAEGDLAGHRI
HHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHEEECCHHHHHHHHCCCEEECCCCCCCEE
VSRDWALAPGKSPNPTDALAYFDGWWIPPNHGRDRDFAAIGIYGQFVYVNPSTHTTIVKL
ECCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEEEE
SEYGAEQDELDTLLAMRQIAHSITAAQ
HHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIKKSVSIAAIASLCLLIVSWNLLRLPSPIVLLDLEFGWTPTSQTFRLFQNRLLEKSAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
PLVLNTAEPRFNHAVTWKGADSNVDDFLKNTQTRAFLVIQDGKIAYERYANGYSAASKFA
CEEEECCCCCCCCEEEECCCCCCHHHHHHCCCCEEEEEEECCCEEHHHHCCCHHHHHHHH
SYSVAKSFVATMVGAALFDGRIHSLQDPIGNYLEPGDVREQYRRVTIEQLLSMRSGIDAA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
ESYDSAFAPVVQMYLTTDLNRFLSRVSGFRHPPGQEFEYRSVDTLVLSKVLTRATGVPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
AYAQQKLWTPLGASSDASWSLDSATHGVEKSFCCLNATARDFAKLGLLYLAEGDLAGHRI
HHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHEEECCHHHHHHHHCCCEEECCCCCCCEE
VSRDWALAPGKSPNPTDALAYFDGWWIPPNHGRDRDFAAIGIYGQFVYVNPSTHTTIVKL
ECCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEEEE
SEYGAEQDELDTLLAMRQIAHSITAAQ
HHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]