Click here to switch to the map view.

The map label for this gene is emrA [H]

Identifier: 238024016

GI number: 238024016

Start: 685824

End: 687014

Strand: Direct

Name: emrA [H]

Synonym: bglu_2g05700

Alternate gene names: 238024016

Gene position: 685824-687014 (Clockwise)

Preceding gene: 238024015

Following gene: 238024017

Centisome position: 24.26

GC content: 73.55

Gene sequence:

>1191_bases
ATGCACGACGACAACCACGAGGCGCCCCGCCACGGCGCGAGCGCCACGCCCGAGGCGGGCGGGCTGCAGGCCAGGCGCGC
CGCGCGCCGCCGCCATTTCGCATTGTTCTTCGGCGTGGTCGCGGTGGCCGCGATCGCGGCGGCCGCCGACTGGTATCTGA
GCGGCCGCTACCATGAGGAGACCGACGACGCCTACGTGGCCGGCAACATCGTGCAGGTGGCCGCGCAGGTGCCGGGCACC
GTGGTCGACGTGCTGGTCAGCGACACGCAGCGCGTGCGCGCGGGCCAGACGCTGGTGCGCCTGGACGACGCCGAGGCGTC
GGTGGCGCTCGCGCAGGCGCGGGCCCAGCTCGCGCAGGCGGTGCGCCAGGCCGCCAGCTCGCGCGTGTCGAATGCGATGT
ATGCCGACGCGGTCAGCGCGCGCGAGGCCGATCTCGAAGTCGCGCGGCGTGCCTACGAGGCACGCGCCAATGCCAGCGTC
GAGGTGGTCTCGCCCGAGGAACTCGCGCGCGCGCGGGCTCAGATGATCGGCGCGCAGGCGCAACTGGCGGCGGCGCGCGC
GCAGCTCGAATCGGCGCGCGCGCTCGGCAGCCGGCTCGCGGTGGAGGACGACCCCGCCGTGGTGCAGGCCGCCGCGCAGT
TCAAGCTCGCCTGGCGCAACCTGAAGCGCACCGCGATCGTCGCGCCGGTGGACGGCACGGTGGCGCAGCGCTCGGTGCAG
GTGGGCGCGCAGGTCGGCCCGGGCGTGCCGCTGCTGTCGCTGATCCAGCTCAACGCGCTGTGGATCGAGGCGAACTTCAA
GGAAGGGCAGATCCGCAACATGCGCGCCGGCCAGCCGGTCAGCATCGTGTCGGACATCTATGGCGCGAAGGTGGTCTATC
ACGGGCGCGTGGAGGGCTTCTCGGCCGGCACCGGCAGCGCGTTCTCGATGCTGCCGTCGCAGAACGCGGCCGGCAACTGG
ATCAAGGTGGTGCAGCGCGTGCCGGTGGTGGTGTCGATCGACGCGGCCGATCTCGCCGCGCATCCGCTGCGCATCGGGCT
GTCGATGCGCGTGGTGGTGGACACGCACCAGCGCGGCGGCCACCTGCTCGACAACGAGCAGCCGCTGCCGGGACAGAGCA
CGCGCGTGCACGACGACGTGGCGGCCGAAGCCGCCGCGGCCGCCGCCGCGGTAATCCGCGCGAACCGGTAG

Upstream 100 bases:

>100_bases
GGCTTCGACGCGAGCCAGCTGCCGGGGCCCGCCATCGTCGGCTCCGCCCCCGCTTCTGCCCCCCACGACCGACCGACCGA
TTCGCATTTCGACTGAGCGC

Downstream 100 bases:

>100_bases
GCGAGCGCGGCCGCGCCTCGCATCGCGTGCCCGCTCGCCGGGCACGCGCGAGCTGCGTCGTTCCCGGCGATTTCCATCCG
GCCCGCGCCGCGCGGTGCCG

Product: Secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MHDDNHEAPRHGASATPEAGGLQARRAARRRHFALFFGVVAVAAIAAAADWYLSGRYHEETDDAYVAGNIVQVAAQVPGT
VVDVLVSDTQRVRAGQTLVRLDDAEASVALAQARAQLAQAVRQAASSRVSNAMYADAVSAREADLEVARRAYEARANASV
EVVSPEELARARAQMIGAQAQLAAARAQLESARALGSRLAVEDDPAVVQAAAQFKLAWRNLKRTAIVAPVDGTVAQRSVQ
VGAQVGPGVPLLSLIQLNALWIEANFKEGQIRNMRAGQPVSIVSDIYGAKVVYHGRVEGFSAGTGSAFSMLPSQNAAGNW
IKVVQRVPVVVSIDAADLAAHPLRIGLSMRVVVDTHQRGGHLLDNEQPLPGQSTRVHDDVAAEAAAAAAAVIRANR

Sequences:

>Translated_396_residues
MHDDNHEAPRHGASATPEAGGLQARRAARRRHFALFFGVVAVAAIAAAADWYLSGRYHEETDDAYVAGNIVQVAAQVPGT
VVDVLVSDTQRVRAGQTLVRLDDAEASVALAQARAQLAQAVRQAASSRVSNAMYADAVSAREADLEVARRAYEARANASV
EVVSPEELARARAQMIGAQAQLAAARAQLESARALGSRLAVEDDPAVVQAAAQFKLAWRNLKRTAIVAPVDGTVAQRSVQ
VGAQVGPGVPLLSLIQLNALWIEANFKEGQIRNMRAGQPVSIVSDIYGAKVVYHGRVEGFSAGTGSAFSMLPSQNAAGNW
IKVVQRVPVVVSIDAADLAAHPLRIGLSMRVVVDTHQRGGHLLDNEQPLPGQSTRVHDDVAAEAAAAAAAVIRANR
>Mature_396_residues
MHDDNHEAPRHGASATPEAGGLQARRAARRRHFALFFGVVAVAAIAAAADWYLSGRYHEETDDAYVAGNIVQVAAQVPGT
VVDVLVSDTQRVRAGQTLVRLDDAEASVALAQARAQLAQAVRQAASSRVSNAMYADAVSAREADLEVARRAYEARANASV
EVVSPEELARARAQMIGAQAQLAAARAQLESARALGSRLAVEDDPAVVQAAAQFKLAWRNLKRTAIVAPVDGTVAQRSVQ
VGAQVGPGVPLLSLIQLNALWIEANFKEGQIRNMRAGQPVSIVSDIYGAKVVYHGRVEGFSAGTGSAFSMLPSQNAAGNW
IKVVQRVPVVVSIDAADLAAHPLRIGLSMRVVVDTHQRGGHLLDNEQPLPGQSTRVHDDVAAEAAAAAAAVIRANR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=345, Percent_Identity=43.4782608695652, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1788711, Length=332, Percent_Identity=43.3734939759036, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1790519, Length=311, Percent_Identity=29.5819935691318, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081951, Length=306, Percent_Identity=26.4705882352941, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789637, Length=314, Percent_Identity=25.4777070063694, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41798; Mature: 41798

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDDNHEAPRHGASATPEAGGLQARRAARRRHFALFFGVVAVAAIAAAADWYLSGRYHEE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TDDAYVAGNIVQVAAQVPGTVVDVLVSDTQRVRAGQTLVRLDDAEASVALAQARAQLAQA
CCCCEEHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHH
VRQAASSRVSNAMYADAVSAREADLEVARRAYEARANASVEVVSPEELARARAQMIGAQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
QLAAARAQLESARALGSRLAVEDDPAVVQAAAQFKLAWRNLKRTAIVAPVDGTVAQRSVQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
VGAQVGPGVPLLSLIQLNALWIEANFKEGQIRNMRAGQPVSIVSDIYGAKVVYHGRVEGF
HCCCCCCCCHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCC
SAGTGSAFSMLPSQNAAGNWIKVVQRVPVVVSIDAADLAAHPLRIGLSMRVVVDTHQRGG
CCCCCCHHEECCCCCCCCHHHHHHHHCCEEEEECHHHHHCCCCEECCEEEEEEECCCCCC
HLLDNEQPLPGQSTRVHDDVAAEAAAAAAAVIRANR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHDDNHEAPRHGASATPEAGGLQARRAARRRHFALFFGVVAVAAIAAAADWYLSGRYHEE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TDDAYVAGNIVQVAAQVPGTVVDVLVSDTQRVRAGQTLVRLDDAEASVALAQARAQLAQA
CCCCEEHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHH
VRQAASSRVSNAMYADAVSAREADLEVARRAYEARANASVEVVSPEELARARAQMIGAQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
QLAAARAQLESARALGSRLAVEDDPAVVQAAAQFKLAWRNLKRTAIVAPVDGTVAQRSVQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
VGAQVGPGVPLLSLIQLNALWIEANFKEGQIRNMRAGQPVSIVSDIYGAKVVYHGRVEGF
HCCCCCCCCHHHHHHHEEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCC
SAGTGSAFSMLPSQNAAGNWIKVVQRVPVVVSIDAADLAAHPLRIGLSMRVVVDTHQRGG
CCCCCCHHEECCCCCCCCHHHHHHHHCCEEEEECHHHHHCCCCEECCEEEEEEECCCCCC
HLLDNEQPLPGQSTRVHDDVAAEAAAAAAAVIRANR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]