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The map label for this gene is emrB [H]

Identifier: 238024013

GI number: 238024013

Start: 681572

End: 683230

Strand: Direct

Name: emrB [H]

Synonym: bglu_2g05670

Alternate gene names: 238024013

Gene position: 681572-683230 (Clockwise)

Preceding gene: 238024012

Following gene: 238024014

Centisome position: 24.11

GC content: 69.02

Gene sequence:

>1659_bases
ATGAGTGTCTCCCCGCTGGCCGCTGCCGATAGCGCCCCGCCCGAAGCGGCCCTGCCCGATGCCACCGGCGCCATCGGCGG
CGTGAGCGGCATGAACGCCGCGCTGCCGCCGCCTGCCGGGCCCGAGCCCGCCGACGCCGCGCCGCCCATGCGCGGCGCGC
GGCTCGCGCTGCTGACCTTCGCGCTGTCGCTCGCCACCTTCATCGAGGTGCTCGATTCGACCGTGACCAACGTCGCCGTG
CCGTCGATCTCGGGCAGTCTCGGCGTGTCGAACAGCCAGGGCACCTGGGTGATCAGCTCGTACTCGGTGGCCGCCGCGAT
CGCGGTGCCGCTGACCGGCTGGCTCGCGCGCCGGGTCGGCGAGCGGCGCCTGTTCGTCACCTCGATCGTGCTGTTTACGC
TGACCTCGCTGCTGTGCGGGCTGGCCGGCGACCTGCGCGTGCTGGTGGCCTGCCGCGCGCTGCAGGGCCTGTTCTCGGGG
CCGATGGTGCCGCTCTCGCAGACCATCCTGATGCGTGCGTTCCCGCCCGAGCGGCGCACCCTCGCGCTCGCGCTGTGGGC
GATGACGGTGCTGCTCGCGCCGATCCTCGGTCCGGTGGCGGGCGGCTGGATCGTCGACAACTATTCTTGGCCATGGATCT
TCCTCGTCAACCTGCCGATCGGGCTGTTCTCGTTCACGGTCTGCGTGACCCTGCTCAAGCCGGCCCGCGAGGGCGGCCAC
GCCGGCCCGATCGACGTGGTGGGCATCGTGCTGCTGGTGATCGGGGTGGGCGCGCTGCAGGCCATGCTCGACCTGGGCCA
CGACCGCGGCTGGTTCGATTCCACGCTGATCTGCGCGCTGGCCGTCACGGCCGGCATCGCGATCGCCTCGCTGCTGATCT
GGGAGCGCGGCGAGGCGCATCCGGTGGTGGACCTGAGCCTGTTCCGCGACCGCACCTTTTCGTTCTGCGTGCTGATCATC
TCGCTCGGCATGATGGCGTTCTCGGTGGTGGGGGTGGTGTTCCCGCTCTGGCTGCAGACCGTGATGGGCTACAACGCGTT
CCATTCCGGGCTCGCGGCCGCACCGCTCGGCATCCTCGCGTTCGTGTTCTCGATCCTGGTGGGGCTCTATTCGAGCCGCT
TCGACGCGCGCGTGCTGGCCACCTTCGGCTTCCTGGTGTTCGCCGGCGTGCAATGGAACAACGCCCACTTCACGCTGACC
ATGACCTTCACGCAGATCATCACGCCGGGGCTGATCCAGGGCATGGGGCTGCCGTGCTTCTTCATTCCGCTCACGGCCGC
CACGCTGTCGCGGATTCCCGACGACAAGCTCGCGGCGGCCTCCAGCCTGTCGAACTTCCTGCGCACGCTGTCGGCCGCGT
TCGGCACCGCCATGAGCGTGACGCTATGGCAGAACCGCGCCACTTACCATTACGACGTGGTCTCGCAGGCGGTGACGAAG
TCGGACGGCAACACGCAGCGCTATGTCGACGCGCTGCACGCGATGGGCATGACCGGCACGCGCGAGCTGGTCTCGCTGAG
CAACGTGGTGCAGAAGCAGGCCTACATGATGGCCACCGGCGACATGTATTTCATGGCCAGCATGACGTGCCTGACGCTGG
CCTGCCTGATGTGGCTGACGCGGCCCAGGCGCGGCGCGGCCATGACGATCGGACATTGA

Upstream 100 bases:

>100_bases
AGCGGATCGCGCCGGGCGGCGGCATCGACGGCGAACCTGCGCCGCGAGGCTGCCGCGGTGCCACCGGCCCCGGCCTGGCC
GCACCGGAGGCGTCGGGCGC

Downstream 100 bases:

>100_bases
GGCGGGGAGGCGAGGTGAGCAGCCGGACCCTGCCGGAACACATGCCCGCCGCGGCGCGGGCCAACCCTGGTGGCATGAGA
TGCGGAGCAAGGCGATGAGG

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSVSPLAAADSAPPEAALPDATGAIGGVSGMNAALPPPAGPEPADAAPPMRGARLALLTFALSLATFIEVLDSTVTNVAV
PSISGSLGVSNSQGTWVISSYSVAAAIAVPLTGWLARRVGERRLFVTSIVLFTLTSLLCGLAGDLRVLVACRALQGLFSG
PMVPLSQTILMRAFPPERRTLALALWAMTVLLAPILGPVAGGWIVDNYSWPWIFLVNLPIGLFSFTVCVTLLKPAREGGH
AGPIDVVGIVLLVIGVGALQAMLDLGHDRGWFDSTLICALAVTAGIAIASLLIWERGEAHPVVDLSLFRDRTFSFCVLII
SLGMMAFSVVGVVFPLWLQTVMGYNAFHSGLAAAPLGILAFVFSILVGLYSSRFDARVLATFGFLVFAGVQWNNAHFTLT
MTFTQIITPGLIQGMGLPCFFIPLTAATLSRIPDDKLAAASSLSNFLRTLSAAFGTAMSVTLWQNRATYHYDVVSQAVTK
SDGNTQRYVDALHAMGMTGTRELVSLSNVVQKQAYMMATGDMYFMASMTCLTLACLMWLTRPRRGAAMTIGH

Sequences:

>Translated_552_residues
MSVSPLAAADSAPPEAALPDATGAIGGVSGMNAALPPPAGPEPADAAPPMRGARLALLTFALSLATFIEVLDSTVTNVAV
PSISGSLGVSNSQGTWVISSYSVAAAIAVPLTGWLARRVGERRLFVTSIVLFTLTSLLCGLAGDLRVLVACRALQGLFSG
PMVPLSQTILMRAFPPERRTLALALWAMTVLLAPILGPVAGGWIVDNYSWPWIFLVNLPIGLFSFTVCVTLLKPAREGGH
AGPIDVVGIVLLVIGVGALQAMLDLGHDRGWFDSTLICALAVTAGIAIASLLIWERGEAHPVVDLSLFRDRTFSFCVLII
SLGMMAFSVVGVVFPLWLQTVMGYNAFHSGLAAAPLGILAFVFSILVGLYSSRFDARVLATFGFLVFAGVQWNNAHFTLT
MTFTQIITPGLIQGMGLPCFFIPLTAATLSRIPDDKLAAASSLSNFLRTLSAAFGTAMSVTLWQNRATYHYDVVSQAVTK
SDGNTQRYVDALHAMGMTGTRELVSLSNVVQKQAYMMATGDMYFMASMTCLTLACLMWLTRPRRGAAMTIGH
>Mature_551_residues
SVSPLAAADSAPPEAALPDATGAIGGVSGMNAALPPPAGPEPADAAPPMRGARLALLTFALSLATFIEVLDSTVTNVAVP
SISGSLGVSNSQGTWVISSYSVAAAIAVPLTGWLARRVGERRLFVTSIVLFTLTSLLCGLAGDLRVLVACRALQGLFSGP
MVPLSQTILMRAFPPERRTLALALWAMTVLLAPILGPVAGGWIVDNYSWPWIFLVNLPIGLFSFTVCVTLLKPAREGGHA
GPIDVVGIVLLVIGVGALQAMLDLGHDRGWFDSTLICALAVTAGIAIASLLIWERGEAHPVVDLSLFRDRTFSFCVLIIS
LGMMAFSVVGVVFPLWLQTVMGYNAFHSGLAAAPLGILAFVFSILVGLYSSRFDARVLATFGFLVFAGVQWNNAHFTLTM
TFTQIITPGLIQGMGLPCFFIPLTAATLSRIPDDKLAAASSLSNFLRTLSAAFGTAMSVTLWQNRATYHYDVVSQAVTKS
DGNTQRYVDALHAMGMTGTRELVSLSNVVQKQAYMMATGDMYFMASMTCLTLACLMWLTRPRRGAAMTIGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=495, Percent_Identity=43.8383838383838, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1788710, Length=498, Percent_Identity=44.1767068273092, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1790195, Length=437, Percent_Identity=29.5194508009153, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788392, Length=419, Percent_Identity=31.2649164677804, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87081983, Length=427, Percent_Identity=26.463700234192, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI87082320, Length=199, Percent_Identity=25.6281407035176, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=236, Percent_Identity=24.5762711864407, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=145, Percent_Identity=26.2068965517241, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58509; Mature: 58377

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPLAAADSAPPEAALPDATGAIGGVSGMNAALPPPAGPEPADAAPPMRGARLALLTF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ALSLATFIEVLDSTVTNVAVPSISGSLGVSNSQGTWVISSYSVAAAIAVPLTGWLARRVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHC
ERRLFVTSIVLFTLTSLLCGLAGDLRVLVACRALQGLFSGPMVPLSQTILMRAFPPERRT
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHH
LALALWAMTVLLAPILGPVAGGWIVDNYSWPWIFLVNLPIGLFSFTVCVTLLKPAREGGH
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC
AGPIDVVGIVLLVIGVGALQAMLDLGHDRGWFDSTLICALAVTAGIAIASLLIWERGEAH
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVVDLSLFRDRTFSFCVLIISLGMMAFSVVGVVFPLWLQTVMGYNAFHSGLAAAPLGILA
CEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHH
FVFSILVGLYSSRFDARVLATFGFLVFAGVQWNNAHFTLTMTFTQIITPGLIQGMGLPCF
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCEEEEEEEHHHHHCHHHHHCCCCCCH
FIPLTAATLSRIPDDKLAAASSLSNFLRTLSAAFGTAMSVTLWQNRATYHYDVVSQAVTK
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHC
SDGNTQRYVDALHAMGMTGTRELVSLSNVVQKQAYMMATGDMYFMASMTCLTLACLMWLT
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
RPRRGAAMTIGH
CCCCCCEEECCC
>Mature Secondary Structure 
SVSPLAAADSAPPEAALPDATGAIGGVSGMNAALPPPAGPEPADAAPPMRGARLALLTF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ALSLATFIEVLDSTVTNVAVPSISGSLGVSNSQGTWVISSYSVAAAIAVPLTGWLARRVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHC
ERRLFVTSIVLFTLTSLLCGLAGDLRVLVACRALQGLFSGPMVPLSQTILMRAFPPERRT
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHH
LALALWAMTVLLAPILGPVAGGWIVDNYSWPWIFLVNLPIGLFSFTVCVTLLKPAREGGH
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCC
AGPIDVVGIVLLVIGVGALQAMLDLGHDRGWFDSTLICALAVTAGIAIASLLIWERGEAH
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVVDLSLFRDRTFSFCVLIISLGMMAFSVVGVVFPLWLQTVMGYNAFHSGLAAAPLGILA
CEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHH
FVFSILVGLYSSRFDARVLATFGFLVFAGVQWNNAHFTLTMTFTQIITPGLIQGMGLPCF
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCEEEEEEEHHHHHCHHHHHCCCCCCH
FIPLTAATLSRIPDDKLAAASSLSNFLRTLSAAFGTAMSVTLWQNRATYHYDVVSQAVTK
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHC
SDGNTQRYVDALHAMGMTGTRELVSLSNVVQKQAYMMATGDMYFMASMTCLTLACLMWLT
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
RPRRGAAMTIGH
CCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]