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The map label for this gene is opuAB [H]

Identifier: 238023929

GI number: 238023929

Start: 574642

End: 575535

Strand: Direct

Name: opuAB [H]

Synonym: bglu_2g04780

Alternate gene names: 238023929

Gene position: 574642-575535 (Clockwise)

Preceding gene: 238023928

Following gene: 238023930

Centisome position: 20.32

GC content: 67.67

Gene sequence:

>894_bases
ATGTCTGAACTGATTCCGCTCGGCAGCTGGGTCGACCAGTCGGTCCGTTACCTGCTCGACCACGACGCGAAGACGTTCGA
CGCGATCGGCCGGGTGATCGAAAGCCTCGCCGCGCTGGTCGAGCACGGCTTGCAGGCGATTCCGATGTGGCTGCTGATGG
CGATCTTCATCGGCATCGGGCTGTGGCGCGTGGGCTGGCGCTTCGCGATCTTCACCACGCTCTCGCTGCTGCTGATCTTC
GCCACCGGCTTCTGGGACCAGACGGTCGTCACGCTCGGCCTCACGCTGTCGTCCACCATCATCAGCCTCGTGCTGGGCAT
CCCGCTCGGCGTGTGGGCGGCCAAGAGCAAGTGGGTGTCGGCCATCGTGCGGCCGATCCTGGACCTGATGCAGACCATGC
CGGCGTTCGTCTACCTGATCCCGGCCGCGATGCTGTTCGGTCTCGGCCGCGTGCCGGGGATCCTGTCCACGGTGATCTTC
GCGATGCCGCCGGCGGTGCGCCTGACGAGCCTCGGCATCCGCCACGTCAACCGCGAGATCGTCGAGGCCGGCCAGGCGTT
CGGCTGCACGCCATGGCAGCTGCTCTACAAGGTGCAGTTCCCGAACGCGCTGCCGTCGATCATGCAGGGCGTGAACCAGA
CCATCATGATGGCGCTGTCGATGGTGATCATCGCCTCGATGGTGGGCGCGGGCGGCCTCGGCAACGACGTGCTCGCCAGC
ATCCAGCGCCTCGACATCGGCCTCGGCTTCGAGAGCGGGCTGTCGGTGGTGCTGCTCGCCATCATCCTGGACCGCATCAC
GGAAAGCTTCGGCCGCGCCCCCGGTGCCGCGCGCGCGCCGCTGTTCGCGGGCTTCCGGCAGCTGTTCGGCAGCAAGGCCG
CGGCGCAGGCCTGA

Upstream 100 bases:

>100_bases
AGCCCGAATGCACTGCCCGTCGTCGATGGCGACGGCCGTTACTGCGGCTCCGTCGATCGGGCCGTGCTCCTGAAGGCCAT
TACGCGTTCGCGAGGCTCCC

Downstream 100 bases:

>100_bases
GCCTGCCGCCCCGGTTCGTCTGATCCGTTTCGTTTCTGTTGCCGGTGCCGGTGGCCACGCGCCGCCGGCACCGGCTTCGT
GCAACCTGCTCATGTCGGGA

Product: choline ABC transporter membrane-spanning permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSELIPLGSWVDQSVRYLLDHDAKTFDAIGRVIESLAALVEHGLQAIPMWLLMAIFIGIGLWRVGWRFAIFTTLSLLLIF
ATGFWDQTVVTLGLTLSSTIISLVLGIPLGVWAAKSKWVSAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIF
AMPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGFRQLFGSKAAAQA

Sequences:

>Translated_297_residues
MSELIPLGSWVDQSVRYLLDHDAKTFDAIGRVIESLAALVEHGLQAIPMWLLMAIFIGIGLWRVGWRFAIFTTLSLLLIF
ATGFWDQTVVTLGLTLSSTIISLVLGIPLGVWAAKSKWVSAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIF
AMPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGFRQLFGSKAAAQA
>Mature_296_residues
SELIPLGSWVDQSVRYLLDHDAKTFDAIGRVIESLAALVEHGLQAIPMWLLMAIFIGIGLWRVGWRFAIFTTLSLLLIFA
TGFWDQTVVTLGLTLSSTIISLVLGIPLGVWAAKSKWVSAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFA
MPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLASI
QRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGFRQLFGSKAAAQA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=271, Percent_Identity=46.1254612546125, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1788451, Length=249, Percent_Identity=26.9076305220884, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788449, Length=180, Percent_Identity=27.2222222222222, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31963; Mature: 31831

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELIPLGSWVDQSVRYLLDHDAKTFDAIGRVIESLAALVEHGLQAIPMWLLMAIFIGIG
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWRVGWRFAIFTTLSLLLIFATGFWDQTVVTLGLTLSSTIISLVLGIPLGVWAAKSKWVS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFAMPPAVRLTSLGIRHVNREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGFRQLFGSKAAAQA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
SELIPLGSWVDQSVRYLLDHDAKTFDAIGRVIESLAALVEHGLQAIPMWLLMAIFIGIG
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWRVGWRFAIFTTLSLLLIFATGFWDQTVVTLGLTLSSTIISLVLGIPLGVWAAKSKWVS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFAMPPAVRLTSLGIRHVNREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGFRQLFGSKAAAQA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]