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The map label for this gene is nadE [H]

Identifier: 238023879

GI number: 238023879

Start: 501578

End: 502441

Strand: Reverse

Name: nadE [H]

Synonym: bglu_2g04180

Alternate gene names: 238023879

Gene position: 502441-501578 (Counterclockwise)

Preceding gene: 238023880

Following gene: 238023878

Centisome position: 17.77

GC content: 71.41

Gene sequence:

>864_bases
ATGACCCGTCCCGACTACGCCGCGCGGCAACGCGCGATCGCCGCCGAACTGCTGGTGGCACCGAGCTTCGACGCCGCCGC
CGAGGCCGAGCGCCGCGTCGCCTTCCTGGCCGACTATCTGCGCTCGACCGGCCTGGCCACCTACGTGCTCGGCATCAGCG
GCGGGGTCGATTCGTCGACCGCCGGCCGGCTCGCGCAACTGGCCGTCGAGCGGCTGCGCGCGAGCGGCTACGATGCGCGC
TTCATCGCCGTGCGGCTGCCCTACGGCGTGCAGCGCGACGAGGAAGACGCGCAGCGCGCGCTGGCCTTCGTGGCCGCCGA
CGAGAACCTGACGATCGACGTCAAGCCCGCCGCCGACGCCATGCTCGGCGCCCTGGTGGCCGCCGGCCAGAGGTTTCGCG
ACGAGGCCCAGCAGGATTTCGTCCACGGCAACGTCAAGGCGCGCGAGCGGATGATCGCGCAGTACGCGGTGGCCGGCGCG
CGCCGCGGCATCGTGATCGGCACCGATCACGCGGCCGAATCGGTGATGGGCTTCTTCACCAAGTTCGGCGACGGCGGCGC
CGACCTCCTGCCGCTCGCCGGCCTGACCAAGCGCCGCGTGCGCGCGGTGGCGCGCCACCTGGGCGGCGCCGAAGCGCTCG
TCATGAAGGTGCCGACCGCCGACCTCGAGGCGCTGCGTCCGCTGCTGCCCGACGAGACCGCCTACGGCGTGACCTACGAT
CAGATCGACGATTTCCTCGAAGGCAAGCCGGTGGACGATCACGTCTACGAAACCGTGCTGCGCTTCTACGAGATGACGCG
CCACAAGCGCGCGCTGCCCTACACGCCGTTCGACTGGCCGCATCAGCCAGGCGGCGTGGTATGA

Upstream 100 bases:

>100_bases
CGCTGGCGCTGGCCGGGGTCGTGCTGGCGCGGGCGCGCTGAGCCGCGCGTTTGCGCTTATGATGCCCGATCTGACCTGAC
CCGTTCCGGAGCAACCCGAG

Downstream 100 bases:

>100_bases
GCACCGCGCCGCTCGGGGAGGCGCCGTCATGCTGATCGACTGGCAGGCCATCGTCGCCGTGTTGTCCGTCTACGTGGTCG
GCGTGGTCGCGCCCGGCCCG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTRPDYAARQRAIAAELLVAPSFDAAAEAERRVAFLADYLRSTGLATYVLGISGGVDSSTAGRLAQLAVERLRASGYDAR
FIAVRLPYGVQRDEEDAQRALAFVAADENLTIDVKPAADAMLGALVAAGQRFRDEAQQDFVHGNVKARERMIAQYAVAGA
RRGIVIGTDHAAESVMGFFTKFGDGGADLLPLAGLTKRRVRAVARHLGGAEALVMKVPTADLEALRPLLPDETAYGVTYD
QIDDFLEGKPVDDHVYETVLRFYEMTRHKRALPYTPFDWPHQPGGVV

Sequences:

>Translated_287_residues
MTRPDYAARQRAIAAELLVAPSFDAAAEAERRVAFLADYLRSTGLATYVLGISGGVDSSTAGRLAQLAVERLRASGYDAR
FIAVRLPYGVQRDEEDAQRALAFVAADENLTIDVKPAADAMLGALVAAGQRFRDEAQQDFVHGNVKARERMIAQYAVAGA
RRGIVIGTDHAAESVMGFFTKFGDGGADLLPLAGLTKRRVRAVARHLGGAEALVMKVPTADLEALRPLLPDETAYGVTYD
QIDDFLEGKPVDDHVYETVLRFYEMTRHKRALPYTPFDWPHQPGGVV
>Mature_286_residues
TRPDYAARQRAIAAELLVAPSFDAAAEAERRVAFLADYLRSTGLATYVLGISGGVDSSTAGRLAQLAVERLRASGYDARF
IAVRLPYGVQRDEEDAQRALAFVAADENLTIDVKPAADAMLGALVAAGQRFRDEAQQDFVHGNVKARERMIAQYAVAGAR
RGIVIGTDHAAESVMGFFTKFGDGGADLLPLAGLTKRRVRAVARHLGGAEALVMKVPTADLEALRPLLPDETAYGVTYDQ
IDDFLEGKPVDDHVYETVLRFYEMTRHKRALPYTPFDWPHQPGGVV

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=50, Blast_Score=247, Evalue=5e-67,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 31220; Mature: 31089

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPDYAARQRAIAAELLVAPSFDAAAEAERRVAFLADYLRSTGLATYVLGISGGVDSST
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHH
AGRLAQLAVERLRASGYDARFIAVRLPYGVQRDEEDAQRALAFVAADENLTIDVKPAADA
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHEEECCCEEEEECCHHHH
MLGALVAAGQRFRDEAQQDFVHGNVKARERMIAQYAVAGARRGIVIGTDHAAESVMGFFT
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHH
KFGDGGADLLPLAGLTKRRVRAVARHLGGAEALVMKVPTADLEALRPLLPDETAYGVTYD
HHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCHH
QIDDFLEGKPVDDHVYETVLRFYEMTRHKRALPYTPFDWPHQPGGVV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRPDYAARQRAIAAELLVAPSFDAAAEAERRVAFLADYLRSTGLATYVLGISGGVDSST
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHH
AGRLAQLAVERLRASGYDARFIAVRLPYGVQRDEEDAQRALAFVAADENLTIDVKPAADA
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHEEECCCEEEEECCHHHH
MLGALVAAGQRFRDEAQQDFVHGNVKARERMIAQYAVAGARRGIVIGTDHAAESVMGFFT
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHH
KFGDGGADLLPLAGLTKRRVRAVARHLGGAEALVMKVPTADLEALRPLLPDETAYGVTYD
HHCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCHH
QIDDFLEGKPVDDHVYETVLRFYEMTRHKRALPYTPFDWPHQPGGVV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA