Click here to switch to the map view.

The map label for this gene is purR [H]

Identifier: 238023863

GI number: 238023863

Start: 481242

End: 482288

Strand: Reverse

Name: purR [H]

Synonym: bglu_2g04020

Alternate gene names: 238023863

Gene position: 482288-481242 (Counterclockwise)

Preceding gene: 238023870

Following gene: 238023861

Centisome position: 17.06

GC content: 71.63

Gene sequence:

>1047_bases
ATGGTGACGCTCGCCGAAGTCGCGCGCCGCGCGAACGTGACGGCCGCGACCGTCTCGAACGTGCTGCGCAACCCGCAGAA
GGTGAAGACGTCCACCGTCGAGCGCGTGATGGCCGCCATCCGTGAGCTCGGCTACCGGCCGAACCTCACGGCTCGCGCGC
TGGCCGAAGGCCGCACGCCGACGCTCGCGCTGATGCTGTCGAACATCACCAACCCGTTCTATCCGGAGTTCGTGCTGGCG
GCCGAGCGCGAGGCGCGCAAGCGCGGCTACTACCTGCTCGTCACGAACACCGACGACGACCCCGCGATCACGCGCAGTTA
CCTGGAGCGGATTGCCGGCACGCTCGCGGCCGGCGCGATCGTGATGAACACCGATCTCGCCGAAGCCGAGCTGGCCGACC
TCTCGCGCCACGGCGCAGCCATCGTGCTGTGCATGTGGGAGCACCTGCCGGCGTCGCACACGCTGCCCTGCGTGACGGTC
GACTTCGCGGCGGCCGGTGCGCTGGCCGCCGCGCACCTGTCCGAGCTGCGGCACCGGCGCTTCGGCGCGCTGGTGGGCAA
GGGCTCGGGCGGCCCGCAGTCGGTGCGGCTGCGCGGCTTCGTCGATGCGCTGCTGGCGCGCGGCCACGCCGAAGGCGCGG
TCACGACGCTGTCGACGCACGATTCGATCGAGGGCGGCTATGAAGCGACCATGACGCTGCTGCGCGCGCATCCGGAGCTG
ACCGCGCTGTTCGCGACCAACGACCTGATGGCGATCGGCGCGTTGCAGGCGGCGGCCGACCTGGGCCGGCGCGTGCCCGC
CGACCTGTCGGTGGTCGGCATGACGGATACCCAGCTCGCACACCAGTTCCGTCCCGCGCTGACGACGGTGCGGTTCCCGA
CGCCGTTGATCGCCGCGCATGCGATCGCCACGGCGCTCGAGATGATCGAGGGCGAGCTGCCAGGCGCAAGCACGCGCGTG
TGCGGCACACCGGAGCTCGTGGTCAGGGAGTCCACGGCCGTCGCGCCGGCACGCCGCAAGGCGGCATCGGCGCGACGCCC
GAATTGA

Upstream 100 bases:

>100_bases
ACCTTCACTGTGCAACTTGTGGAACGCCATCCCAATATATGCGCGCAGTTTCGTTTTCGTCAACCCTGACCCTCCTGCCG
GCCGCGGCCGACCAGCGCCG

Downstream 100 bases:

>100_bases
AGCGATGGCGCCCGGCCGCGGTCGTCGGCTGCACGGCCCGGCGGCGGCCGGCGCCTCGCCCCGAGCCTGCGCGAGATCGA
AGCGCTGCCTCCGGCAAGCT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MVTLAEVARRANVTAATVSNVLRNPQKVKTSTVERVMAAIRELGYRPNLTARALAEGRTPTLALMLSNITNPFYPEFVLA
AEREARKRGYYLLVTNTDDDPAITRSYLERIAGTLAAGAIVMNTDLAEAELADLSRHGAAIVLCMWEHLPASHTLPCVTV
DFAAAGALAAAHLSELRHRRFGALVGKGSGGPQSVRLRGFVDALLARGHAEGAVTTLSTHDSIEGGYEATMTLLRAHPEL
TALFATNDLMAIGALQAAADLGRRVPADLSVVGMTDTQLAHQFRPALTTVRFPTPLIAAHAIATALEMIEGELPGASTRV
CGTPELVVRESTAVAPARRKAASARRPN

Sequences:

>Translated_348_residues
MVTLAEVARRANVTAATVSNVLRNPQKVKTSTVERVMAAIRELGYRPNLTARALAEGRTPTLALMLSNITNPFYPEFVLA
AEREARKRGYYLLVTNTDDDPAITRSYLERIAGTLAAGAIVMNTDLAEAELADLSRHGAAIVLCMWEHLPASHTLPCVTV
DFAAAGALAAAHLSELRHRRFGALVGKGSGGPQSVRLRGFVDALLARGHAEGAVTTLSTHDSIEGGYEATMTLLRAHPEL
TALFATNDLMAIGALQAAADLGRRVPADLSVVGMTDTQLAHQFRPALTTVRFPTPLIAAHAIATALEMIEGELPGASTRV
CGTPELVVRESTAVAPARRKAASARRPN
>Mature_348_residues
MVTLAEVARRANVTAATVSNVLRNPQKVKTSTVERVMAAIRELGYRPNLTARALAEGRTPTLALMLSNITNPFYPEFVLA
AEREARKRGYYLLVTNTDDDPAITRSYLERIAGTLAAGAIVMNTDLAEAELADLSRHGAAIVLCMWEHLPASHTLPCVTV
DFAAAGALAAAHLSELRHRRFGALVGKGSGGPQSVRLRGFVDALLARGHAEGAVTTLSTHDSIEGGYEATMTLLRAHPEL
TALFATNDLMAIGALQAAADLGRRVPADLSVVGMTDTQLAHQFRPALTTVRFPTPLIAAHAIATALEMIEGELPGASTRV
CGTPELVVRESTAVAPARRKAASARRPN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=348, Percent_Identity=32.183908045977, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=29.6407185628743, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=32.6347305389222, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=33.6734693877551, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1790194, Length=310, Percent_Identity=30, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789068, Length=342, Percent_Identity=29.2397660818713, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=25.7142857142857, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=26.9592476489028, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786540, Length=360, Percent_Identity=26.6666666666667, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787906, Length=271, Percent_Identity=29.8892988929889, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790192, Length=284, Percent_Identity=26.7605633802817, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786268, Length=285, Percent_Identity=27.3684210526316, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36979; Mature: 36979

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLAEVARRANVTAATVSNVLRNPQKVKTSTVERVMAAIRELGYRPNLTARALAEGRTP
CCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCC
TLALMLSNITNPFYPEFVLAAEREARKRGYYLLVTNTDDDPAITRSYLERIAGTLAAGAI
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCEE
VMNTDLAEAELADLSRHGAAIVLCMWEHLPASHTLPCVTVDFAAAGALAAAHLSELRHRR
EEECCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
FGALVGKGSGGPQSVRLRGFVDALLARGHAEGAVTTLSTHDSIEGGYEATMTLLRAHPEL
HHHEEECCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCE
TALFATNDLMAIGALQAAADLGRRVPADLSVVGMTDTQLAHQFRPALTTVRFPTPLIAAH
EEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHEEECCCHHHHHH
AIATALEMIEGELPGASTRVCGTPELVVRESTAVAPARRKAASARRPN
HHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MVTLAEVARRANVTAATVSNVLRNPQKVKTSTVERVMAAIRELGYRPNLTARALAEGRTP
CCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCC
TLALMLSNITNPFYPEFVLAAEREARKRGYYLLVTNTDDDPAITRSYLERIAGTLAAGAI
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCEE
VMNTDLAEAELADLSRHGAAIVLCMWEHLPASHTLPCVTVDFAAAGALAAAHLSELRHRR
EEECCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
FGALVGKGSGGPQSVRLRGFVDALLARGHAEGAVTTLSTHDSIEGGYEATMTLLRAHPEL
HHHEEECCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCE
TALFATNDLMAIGALQAAADLGRRVPADLSVVGMTDTQLAHQFRPALTTVRFPTPLIAAH
EEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHEEECCCHHHHHH
AIATALEMIEGELPGASTRVCGTPELVVRESTAVAPARRKAASARRPN
HHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA