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The map label for this gene is pitA [H]

Identifier: 238023860

GI number: 238023860

Start: 476682

End: 478262

Strand: Reverse

Name: pitA [H]

Synonym: bglu_2g03990

Alternate gene names: 238023860

Gene position: 478262-476682 (Counterclockwise)

Preceding gene: 238023861

Following gene: 238023859

Centisome position: 16.92

GC content: 67.81

Gene sequence:

>1581_bases
ATGAACCAATCCGCGTTACCCACCGCAGATGCCGGCCGCTCACGGCGATTCGGCCAGCTGGTCTTTCTCGCCGTGCTCGT
CGTCGGCGGCGCGTATGTGCTCACTCATCTGATGGCCGATCTCGCGCCGGTCGGCGAGCCTTCGGTGTTCCCCTACGTGC
TGCTCGGCATCGCGCTGCTGATCGCGCTCGGCTTCGAGTTCGTCAACGGCTTCCACGACACGGCCAATGCGGTCGCCACC
GTGATCTACACCCATTCGCTGGCGCCGAACGTGGCCGTGGTCTGGTCCGGACTCTGGAACTTCGCGGGCGTGCTGCTCTC
GAGCGGCGCCGTGGCCTTCGGCATCCTGCAGTTGCTGCCGGTGGAGCTGATCCTGCAGGTCGGCAGCGGCGCCGGCTTCG
CGATGGTGTTCGCGCTGCTGATCGCGGCGATCGTCTGGAACCTCACGACCTGGTACTTCGGCCTGCCCTCGTCTAGCTCG
CACACGCTGATCGGCTCGATCATCGGCGTCGGCGTGATGAACCAGCTCATGCACGGCGCCTCGGGCACGAGCGGCGTGGA
CTGGAGCCAGGCGCTCGGGGTCGGCAAATCGCTGCTGTTCTCGCCGATCGTCGGCTTCGTGCTCTCCGGCCTGCTGCTGC
TTCTGCTCAAGGGGCTCGTGAAGCTGCCGCAGCTCTATCGGGAACCGCCGAAGGACAAGACGCCGCCGTTCTGGATTCGC
TGCCTGCTGATCCTGACCTGCACCGGCGTATCGTTCGCGCACGGCTCGAACGACGGCCAGAAGGGCATGGGGCTGATCAT
GCTGATCCTGATCGGCACGGTGCCCACCGCCTTCGCGCTGAACAAGGCGGTCACGCCGGCCGAATCGCAGACCTTTATCG
CCGTCGCGCATCAGGCGGCCGCGACCTTCGGCAAGTACACCAACGGCGCCGCGGCGCCCAGCAACTCGCGCCGCGTGGTC
GAGACCTACCTGCAGACGCACCGGATGACGCCGGACACCGTGCCCGCCGTGCAGCAGTTGTCGAACTCGCTCGCGGCCGC
GGTCGGCTCGACCGGCTCGATCGCGAGCACGCCGCAGGGCGACGTCGACAACCTGCGCAACACCATGTACCTCGTGTCGG
AGGCGATCCGCCTGATGGACAAGGCCGGGCAGCCGGCCTTCTCGGCCGACGATCGGGCCGCGATCAACAACTATCGCGCG
CAGCTCGACCACGCCACCAAGTTCATCCCGACCTGGGTCAAGGTCGCCGTCGCGCTCGCGCTCGGCCTCGGCACGATGGT
GGGCTGGAGGCGGATCGTGGTCACCGTGGGCGAGAAGATCGGCAAGCAGCATCTGACCTACGGCCAGGGCGCCTCGGCCG
AAGTCGTCGCGATGGTCACGATCGGCGCGGCCGACATGTACGGGCTGCCGGTCTCGACCACCCATGTGCTGTCGTCGGGC
GTGGCAGGCACGATGGCCGCCAACGGCTCGGGCCTGCAATGGTCGACCGTGCGCAGCCTGGTGCTGGCCTGGCTGCTGAC
GCTGCCGGCCTCGATCGTGCTGGCCGGCGCGCTCTACTGGGTGTTCCACGCGCTGCTCTGA

Upstream 100 bases:

>100_bases
TGGAAAGATTGCGGGTGGCACCGCGCCATGTCATCAAACTGCCTGAGTCGCGGCGCTAGGATCGGGTGATCGACTCCTTT
CACTCCCCTCCCGGCCCGCC

Downstream 100 bases:

>100_bases
GCCCGCGCGGCGTGGCGCGGGCCGCGCCCGCCTCGGCCCGGCCCCTGGCCCGGCGCGGCAGCCGGAGTTGCCGCGCAAGA
AACGGAGCGGCACGCGCGCG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNQSALPTADAGRSRRFGQLVFLAVLVVGGAYVLTHLMADLAPVGEPSVFPYVLLGIALLIALGFEFVNGFHDTANAVAT
VIYTHSLAPNVAVVWSGLWNFAGVLLSSGAVAFGILQLLPVELILQVGSGAGFAMVFALLIAAIVWNLTTWYFGLPSSSS
HTLIGSIIGVGVMNQLMHGASGTSGVDWSQALGVGKSLLFSPIVGFVLSGLLLLLLKGLVKLPQLYREPPKDKTPPFWIR
CLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAFALNKAVTPAESQTFIAVAHQAAATFGKYTNGAAAPSNSRRVV
ETYLQTHRMTPDTVPAVQQLSNSLAAAVGSTGSIASTPQGDVDNLRNTMYLVSEAIRLMDKAGQPAFSADDRAAINNYRA
QLDHATKFIPTWVKVAVALALGLGTMVGWRRIVVTVGEKIGKQHLTYGQGASAEVVAMVTIGAADMYGLPVSTTHVLSSG
VAGTMAANGSGLQWSTVRSLVLAWLLTLPASIVLAGALYWVFHALL

Sequences:

>Translated_526_residues
MNQSALPTADAGRSRRFGQLVFLAVLVVGGAYVLTHLMADLAPVGEPSVFPYVLLGIALLIALGFEFVNGFHDTANAVAT
VIYTHSLAPNVAVVWSGLWNFAGVLLSSGAVAFGILQLLPVELILQVGSGAGFAMVFALLIAAIVWNLTTWYFGLPSSSS
HTLIGSIIGVGVMNQLMHGASGTSGVDWSQALGVGKSLLFSPIVGFVLSGLLLLLLKGLVKLPQLYREPPKDKTPPFWIR
CLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAFALNKAVTPAESQTFIAVAHQAAATFGKYTNGAAAPSNSRRVV
ETYLQTHRMTPDTVPAVQQLSNSLAAAVGSTGSIASTPQGDVDNLRNTMYLVSEAIRLMDKAGQPAFSADDRAAINNYRA
QLDHATKFIPTWVKVAVALALGLGTMVGWRRIVVTVGEKIGKQHLTYGQGASAEVVAMVTIGAADMYGLPVSTTHVLSSG
VAGTMAANGSGLQWSTVRSLVLAWLLTLPASIVLAGALYWVFHALL
>Mature_526_residues
MNQSALPTADAGRSRRFGQLVFLAVLVVGGAYVLTHLMADLAPVGEPSVFPYVLLGIALLIALGFEFVNGFHDTANAVAT
VIYTHSLAPNVAVVWSGLWNFAGVLLSSGAVAFGILQLLPVELILQVGSGAGFAMVFALLIAAIVWNLTTWYFGLPSSSS
HTLIGSIIGVGVMNQLMHGASGTSGVDWSQALGVGKSLLFSPIVGFVLSGLLLLLLKGLVKLPQLYREPPKDKTPPFWIR
CLLILTCTGVSFAHGSNDGQKGMGLIMLILIGTVPTAFALNKAVTPAESQTFIAVAHQAAATFGKYTNGAAAPSNSRRVV
ETYLQTHRMTPDTVPAVQQLSNSLAAAVGSTGSIASTPQGDVDNLRNTMYLVSEAIRLMDKAGQPAFSADDRAAINNYRA
QLDHATKFIPTWVKVAVALALGLGTMVGWRRIVVTVGEKIGKQHLTYGQGASAEVVAMVTIGAADMYGLPVSTTHVLSSG
VAGTMAANGSGLQWSTVRSLVLAWLLTLPASIVLAGALYWVFHALL

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=474, Percent_Identity=44.3037974683544, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1789360, Length=482, Percent_Identity=42.1161825726141, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 55148; Mature: 55148

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure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CCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]