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The map label for this gene is phnV [H]

Identifier: 238023854

GI number: 238023854

Start: 464778

End: 465656

Strand: Direct

Name: phnV [H]

Synonym: bglu_2g03930

Alternate gene names: 238023854

Gene position: 464778-465656 (Clockwise)

Preceding gene: 238023853

Following gene: 339717966

Centisome position: 16.44

GC content: 71.56

Gene sequence:

>879_bases
ATGGCCGCTGACCTGCGCCGGAATGGCGATGCCGCCGGCGGTCCGGCCGGCGTGCCGGCGCCGCGACACGCCGCCGGCTG
GACCGACCCCGTGCTGAAGCATGCCGCGCGCATCGCGCTGGCCTTGGCCGCGTTCGCCTGCCTCTGGCTGTTCGTGCTGC
CGGTGGTGGTGGTCGCGCTGTCGAGCGTGGCCACGCAATGGTCCGGCACGATCCTGCCGACCGGCTACAGCTTGCGCTGG
TTCTCGCGGCTCGGCGAGCCCGAGTTCGATGCGCTCGCCGCCAGCCTCGAGATCGGCTTCGGCGTAGCGGTGATCGGCAC
CGCGCTCGGGCTCTGGCTCGCGCTTGCGCTGGAAGGGCGCGTGCAGGGGCGGGCGGGCGCGCTCGTCGATGCGCTGGTGA
TGCTGCCCAACGGCGTGCCGAGCGTGGTGCTGGGACTTGCGGTGCTGATCGCCTATCATCAAAGACCGGTCGATCTTTCG
AGCTCGGCCGCGATCGTCATGCTCGTGCAACTCGCGCTGGTTCTGCCATTTTGTTACCGATGCGCGGCCGCCGCGCTGCG
GCCCGAATTGACGGTGCTGCGGGAAGCCGCCGCGAGCCTTGGTGCGCCGCCGTCGATGGTGCTGCGCCGCGTGCTGCTGC
CGCAACTCGTGCCGGCGCTGCGCGCGAGCCTGGCGCTCGGCTTCGCGCTGTCGCTCGGCGAGCTGGGCGCGACGATGACG
GTCTATCCGCCCGGCTTCGCCACCGTACCGATCGTCGTGATCGGCGAGGTCGAGCGCGGCTATTACTTGCCGGCTTCGGC
GCTCTCGCTGCTGATGCTGGCGGCTTCGCTCGCGGCGCTGATGCTGATCGCCGCGCGAGTGCCGCGGCTGCGGCGCTGA

Upstream 100 bases:

>100_bases
CTGCCGGCCGCGGCGGCCGGCGCCGTCGTGATGCTCGCCATGTCGCTGTCGCTGTATGTGCTTTACCGCTGGATCAATCG
CCGGGCGATGGGAGGGCGGG

Downstream 100 bases:

>100_bases
TGGATTGGTTGATCGATGGACTGAATGGCTGAATCGGCCCCGTGGATGCCGCGCGCGGCGCGGGCCGCCGGCGCAGATCG
CACGGCGGTGCCGGCGGCCG

Product: binding-protein-dependent transport system protein

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MAADLRRNGDAAGGPAGVPAPRHAAGWTDPVLKHAARIALALAAFACLWLFVLPVVVVALSSVATQWSGTILPTGYSLRW
FSRLGEPEFDALAASLEIGFGVAVIGTALGLWLALALEGRVQGRAGALVDALVMLPNGVPSVVLGLAVLIAYHQRPVDLS
SSAAIVMLVQLALVLPFCYRCAAAALRPELTVLREAAASLGAPPSMVLRRVLLPQLVPALRASLALGFALSLGELGATMT
VYPPGFATVPIVVIGEVERGYYLPASALSLLMLAASLAALMLIAARVPRLRR

Sequences:

>Translated_292_residues
MAADLRRNGDAAGGPAGVPAPRHAAGWTDPVLKHAARIALALAAFACLWLFVLPVVVVALSSVATQWSGTILPTGYSLRW
FSRLGEPEFDALAASLEIGFGVAVIGTALGLWLALALEGRVQGRAGALVDALVMLPNGVPSVVLGLAVLIAYHQRPVDLS
SSAAIVMLVQLALVLPFCYRCAAAALRPELTVLREAAASLGAPPSMVLRRVLLPQLVPALRASLALGFALSLGELGATMT
VYPPGFATVPIVVIGEVERGYYLPASALSLLMLAASLAALMLIAARVPRLRR
>Mature_291_residues
AADLRRNGDAAGGPAGVPAPRHAAGWTDPVLKHAARIALALAAFACLWLFVLPVVVVALSSVATQWSGTILPTGYSLRWF
SRLGEPEFDALAASLEIGFGVAVIGTALGLWLALALEGRVQGRAGALVDALVMLPNGVPSVVLGLAVLIAYHQRPVDLSS
SAAIVMLVQLALVLPFCYRCAAAALRPELTVLREAAASLGAPPSMVLRRVLLPQLVPALRASLALGFALSLGELGATMTV
YPPGFATVPIVVIGEVERGYYLPASALSLLMLAASLAALMLIAARVPRLRR

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=215, Percent_Identity=25.1162790697674, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017661
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30332; Mature: 30200

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADLRRNGDAAGGPAGVPAPRHAAGWTDPVLKHAARIALALAAFACLWLFVLPVVVVAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVATQWSGTILPTGYSLRWFSRLGEPEFDALAASLEIGFGVAVIGTALGLWLALALEGR
HHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VQGRAGALVDALVMLPNGVPSVVLGLAVLIAYHQRPVDLSSSAAIVMLVQLALVLPFCYR
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
CAAAALRPELTVLREAAASLGAPPSMVLRRVLLPQLVPALRASLALGFALSLGELGATMT
HHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VYPPGFATVPIVVIGEVERGYYLPASALSLLMLAASLAALMLIAARVPRLRR
EECCCCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AADLRRNGDAAGGPAGVPAPRHAAGWTDPVLKHAARIALALAAFACLWLFVLPVVVVAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVATQWSGTILPTGYSLRWFSRLGEPEFDALAASLEIGFGVAVIGTALGLWLALALEGR
HHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VQGRAGALVDALVMLPNGVPSVVLGLAVLIAYHQRPVDLSSSAAIVMLVQLALVLPFCYR
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
CAAAALRPELTVLREAAASLGAPPSMVLRRVLLPQLVPALRASLALGFALSLGELGATMT
HHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
VYPPGFATVPIVVIGEVERGYYLPASALSLLMLAASLAALMLIAARVPRLRR
EECCCCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA