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The map label for this gene is phnU [H]

Identifier: 238023853

GI number: 238023853

Start: 463859

End: 464788

Strand: Direct

Name: phnU [H]

Synonym: bglu_2g03920

Alternate gene names: 238023853

Gene position: 463859-464788 (Clockwise)

Preceding gene: 238023852

Following gene: 238023854

Centisome position: 16.41

GC content: 70.0

Gene sequence:

>930_bases
ATGTCGTCCTTATCGAGCCCTGATCTGGACGGCGCGGGCGCCGCCGCGCGCCGCGAGGCGGCGGTGTCCAGTCACGCGCG
CGCGGCGCGCCGGGCCACGCACCGCGCGCGGCTGCATCTGCTGGTGCTGGTGCTGGTGGTGCTCGGGCCGCTCGTGCTCT
ATCCGCTCGCGCGCTTGCTGCTGCTCAGCATGACAGGCGATCATGGCTTGACGCTTGCCGCCTATCGCACGTTCTTCGGC
AATCCCGACACGCGCGGCGTGCTCGGCACCACGCTATCGGTGCTGTGCGTGAGCGCCGGCAGCGCGTCGGTGCTCGGCGT
GCTGCTCGCGGCACTGCTGTTCTTCCGGCCGTTTCCGGGCGCCTCGCTCGTCACGCGCTTCCTCGAGCTCTACGTGGCCT
TCCCCTCGTTCCTGGTCGCCTTCACGCTGATCTTCCTGTACGGCTCGCAGGGCTCGATCAGCATCGCGCTGCAGCGGCTG
TTCCACCTCGATGCGCCGCCGCTCGACTTCCTGTTCGGCATCGGCGGCGTGATCCTGGCGCAGACGGTGTTCTACACGCC
GTTCGTGGTGCGGCCCACGCTCGCCGCGTTCGCCACGCTCGACCTGCGGCTGGTCGAGGCGGCGCGCAGCCTCGGCGCGA
ACGGCTGGATGCTGGCAAGCCGCGTGATTCTGCCGATCGCGTGGCCCGGCATCGCGGCCGGCACGATCCTCTGCTTCCTG
CTCACGCTCAACGAGTTCGGGATCCTGCTCGTGCTCGGCAGCGCGCGGCTGGTCACGCTGCCGGTCGCGATCTACACCAG
TGCGTCGGTCGATCTCGACCTGCCGGCCGCGGCGGCCGGCGCCGTCGTGATGCTCGCCATGTCGCTGTCGCTGTATGTGC
TTTACCGCTGGATCAATCGCCGGGCGATGGGAGGGCGGGATGGCCGCTGA

Upstream 100 bases:

>100_bases
GACGCGGCCGCCGACGAGCCCGTGCGCGTCACCATGCAGCGCGGCCGCGGTCCGACCCCGGAGCGCGGCACGCGCGTTTC
CCTGCATTGCGAGGCCGATG

Downstream 100 bases:

>100_bases
CCTGCGCCGGAATGGCGATGCCGCCGGCGGTCCGGCCGGCGTGCCGGCGCCGCGACACGCCGCCGGCTGGACCGACCCCG
TGCTGAAGCATGCCGCGCGC

Product: 2-aminoethylphosphonate transport system permease PhnU

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSSLSSPDLDGAGAAARREAAVSSHARAARRATHRARLHLLVLVLVVLGPLVLYPLARLLLLSMTGDHGLTLAAYRTFFG
NPDTRGVLGTTLSVLCVSAGSASVLGVLLAALLFFRPFPGASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSISIALQRL
FHLDAPPLDFLFGIGGVILAQTVFYTPFVVRPTLAAFATLDLRLVEAARSLGANGWMLASRVILPIAWPGIAAGTILCFL
LTLNEFGILLVLGSARLVTLPVAIYTSASVDLDLPAAAAGAVVMLAMSLSLYVLYRWINRRAMGGRDGR

Sequences:

>Translated_309_residues
MSSLSSPDLDGAGAAARREAAVSSHARAARRATHRARLHLLVLVLVVLGPLVLYPLARLLLLSMTGDHGLTLAAYRTFFG
NPDTRGVLGTTLSVLCVSAGSASVLGVLLAALLFFRPFPGASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSISIALQRL
FHLDAPPLDFLFGIGGVILAQTVFYTPFVVRPTLAAFATLDLRLVEAARSLGANGWMLASRVILPIAWPGIAAGTILCFL
LTLNEFGILLVLGSARLVTLPVAIYTSASVDLDLPAAAAGAVVMLAMSLSLYVLYRWINRRAMGGRDGR
>Mature_308_residues
SSLSSPDLDGAGAAARREAAVSSHARAARRATHRARLHLLVLVLVVLGPLVLYPLARLLLLSMTGDHGLTLAAYRTFFGN
PDTRGVLGTTLSVLCVSAGSASVLGVLLAALLFFRPFPGASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSISIALQRLF
HLDAPPLDFLFGIGGVILAQTVFYTPFVVRPTLAAFATLDLRLVEAARSLGANGWMLASRVILPIAWPGIAAGTILCFLL
TLNEFGILLVLGSARLVTLPVAIYTSASVDLDLPAAAAGAVVMLAMSLSLYVLYRWINRRAMGGRDGR

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=241, Percent_Identity=25.3112033195021, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786980, Length=233, Percent_Identity=30.4721030042918, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788764, Length=217, Percent_Identity=27.6497695852535, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017636
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32811; Mature: 32680

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSSPDLDGAGAAARREAAVSSHARAARRATHRARLHLLVLVLVVLGPLVLYPLARLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSMTGDHGLTLAAYRTFFGNPDTRGVLGTTLSVLCVSAGSASVLGVLLAALLFFRPFPG
HHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
ASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSISIALQRLFHLDAPPLDFLFGIGGVILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
QTVFYTPFVVRPTLAAFATLDLRLVEAARSLGANGWMLASRVILPIAWPGIAAGTILCFL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHHHHHHHH
LTLNEFGILLVLGSARLVTLPVAIYTSASVDLDLPAAAAGAVVMLAMSLSLYVLYRWINR
HHHHCCCEEEEECCCEEEEEEHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAMGGRDGR
HCCCCCCCC
>Mature Secondary Structure 
SSLSSPDLDGAGAAARREAAVSSHARAARRATHRARLHLLVLVLVVLGPLVLYPLARLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSMTGDHGLTLAAYRTFFGNPDTRGVLGTTLSVLCVSAGSASVLGVLLAALLFFRPFPG
HHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
ASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSISIALQRLFHLDAPPLDFLFGIGGVILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
QTVFYTPFVVRPTLAAFATLDLRLVEAARSLGANGWMLASRVILPIAWPGIAAGTILCFL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCHHHHHHHHHH
LTLNEFGILLVLGSARLVTLPVAIYTSASVDLDLPAAAAGAVVMLAMSLSLYVLYRWINR
HHHHCCCEEEEECCCEEEEEEHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAMGGRDGR
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA