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The map label for this gene is proP [H]

Identifier: 238023838

GI number: 238023838

Start: 446823

End: 448118

Strand: Reverse

Name: proP [H]

Synonym: bglu_2g03770

Alternate gene names: 238023838

Gene position: 448118-446823 (Counterclockwise)

Preceding gene: 238023839

Following gene: 238023836

Centisome position: 15.85

GC content: 68.98

Gene sequence:

>1296_bases
ATGCAAGCATACGAAACGAGCCACGCGCAAACCGCTGCCGCCGAGCGCCCCCTCACGCGCAGCGATTACAAGACCCTCGG
CCTCGCGGCGCTCGGCGGTGCCCTGGAGTTCTACGACTTCATCATCTTCGTGTTCTTCGCACCGGCGATCGGGCAGCTGT
TCTTCCCGCACGACATTCCGGACTGGCTGCGCCAGTTGCAGACTTTCGGCATCTTCGCGGCCGGCTATCTGGCGCGCCCG
CTCGGCGGCATCATCATGGCGCACTTCGGCGACCTGGTCGGGCGCAAGCGGATGTTCACGCTGAGCGTGCTGCTGATGGC
GATCCCGACGCTGCTGATGGGCCTGCTGCCCACCTTCGACACGATCGGCATCCTCGCCCCGGTGCTGCTGCTGCTGTTCC
GCATCCTGCAGGGCGCGGCGGTGGGCGGAGAAGTGCCGGGCGCCTGGGTGTTCGTGTCCGAACACGTGCCCGGCAGCCGC
ATCGGCTACGCCTGCGGCACGCTCACGGCGGGCCTGACGGCCGGCATCCTGCTCGGCTCGCTGGTGGCCGCCAGCGTCAC
CGCGCACTTCTCGCAGGCCGAGATCGCCGCCTACGCGTGGCGCCTGCCGTTCCTGCTCGGCGGCGTGTTCGGCATGTTCT
CCGTCTACCTGCGCCGCTGGCTGCACGAGACGCCGGTGTTCGCCGAGCTGCAGGCGCGCAAGGCGCTGGCCGCCGAGATT
CCGCTCAAGGCCGTGCTGCGCGATCACGGCCGCGCGGTGCTGGTCTCGATGGTGCTGACCTGGATGCTGTCGGCCGCGAT
CGTGGTGGTGATCCTGATGACGCCGACGCTGCTGCAAAAGCAGCTGCACATCCCCGCGGCCACCGCGCTGTTCGCCAACA
GCATCGCCACGCTGTGCCTGACGATCGGCTGCGTGACGGCGGGCGCGCTGGCCGACCGCTTCGGCGCGAAGGCGGTGCTG
GTGGCGGGCTCGCTGCTGTTGGCCGCCAGCTTCTGCACGCTGTATGCGCGGGTGGCCGCCGACGCCTCGACGCTGGTGCC
GCTCTACGCGCTCACCGGCTTCCTGGTGGGCACCGTGGGCGCGGTGCCCACCGCCATGGTGCGCAGTTTCCCGGCCGTGA
TCCGCTTTTCCGGCATCTCGTTCTCGTACAACGTCGCTTACGCGATTTTCGGCGGGCTCTCGCCGGTGATCGTGTCGCTG
ATGATCAAATCGAACCCGTTCGCACCGACCGCCTATGTCGGCGCGGTCTGCGTGATCGGCGCACTCGGCGCGCTTTGCGC
GCGCAGCGTGCGCTGA

Upstream 100 bases:

>100_bases
CGCAGCCCGGGCCGCGCGCCGATCCGGCGCACCGCGCGGGACGCTTGACGGTGTCGCGCGCCGCGCCCGCCGCAAAACTC
GAATATCGATCGTCCAGACC

Downstream 100 bases:

>100_bases
GCGGCGGGAGCCGGCCGCTTCGTGGTCCGCCCGGGGCGCCTCGCGGCGCCCTTTTTCATGCCCGGCGCCCATGCGAGGCG
CGGCCGAGGCGGGACCTCAG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQAYETSHAQTAAAERPLTRSDYKTLGLAALGGALEFYDFIIFVFFAPAIGQLFFPHDIPDWLRQLQTFGIFAAGYLARP
LGGIIMAHFGDLVGRKRMFTLSVLLMAIPTLLMGLLPTFDTIGILAPVLLLLFRILQGAAVGGEVPGAWVFVSEHVPGSR
IGYACGTLTAGLTAGILLGSLVAASVTAHFSQAEIAAYAWRLPFLLGGVFGMFSVYLRRWLHETPVFAELQARKALAAEI
PLKAVLRDHGRAVLVSMVLTWMLSAAIVVVILMTPTLLQKQLHIPAATALFANSIATLCLTIGCVTAGALADRFGAKAVL
VAGSLLLAASFCTLYARVAADASTLVPLYALTGFLVGTVGAVPTAMVRSFPAVIRFSGISFSYNVAYAIFGGLSPVIVSL
MIKSNPFAPTAYVGAVCVIGALGALCARSVR

Sequences:

>Translated_431_residues
MQAYETSHAQTAAAERPLTRSDYKTLGLAALGGALEFYDFIIFVFFAPAIGQLFFPHDIPDWLRQLQTFGIFAAGYLARP
LGGIIMAHFGDLVGRKRMFTLSVLLMAIPTLLMGLLPTFDTIGILAPVLLLLFRILQGAAVGGEVPGAWVFVSEHVPGSR
IGYACGTLTAGLTAGILLGSLVAASVTAHFSQAEIAAYAWRLPFLLGGVFGMFSVYLRRWLHETPVFAELQARKALAAEI
PLKAVLRDHGRAVLVSMVLTWMLSAAIVVVILMTPTLLQKQLHIPAATALFANSIATLCLTIGCVTAGALADRFGAKAVL
VAGSLLLAASFCTLYARVAADASTLVPLYALTGFLVGTVGAVPTAMVRSFPAVIRFSGISFSYNVAYAIFGGLSPVIVSL
MIKSNPFAPTAYVGAVCVIGALGALCARSVR
>Mature_431_residues
MQAYETSHAQTAAAERPLTRSDYKTLGLAALGGALEFYDFIIFVFFAPAIGQLFFPHDIPDWLRQLQTFGIFAAGYLARP
LGGIIMAHFGDLVGRKRMFTLSVLLMAIPTLLMGLLPTFDTIGILAPVLLLLFRILQGAAVGGEVPGAWVFVSEHVPGSR
IGYACGTLTAGLTAGILLGSLVAASVTAHFSQAEIAAYAWRLPFLLGGVFGMFSVYLRRWLHETPVFAELQARKALAAEI
PLKAVLRDHGRAVLVSMVLTWMLSAAIVVVILMTPTLLQKQLHIPAATALFANSIATLCLTIGCVTAGALADRFGAKAVL
VAGSLLLAASFCTLYARVAADASTLVPLYALTGFLVGTVGAVPTAMVRSFPAVIRFSGISFSYNVAYAIFGGLSPVIVSL
MIKSNPFAPTAYVGAVCVIGALGALCARSVR

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=28.436018957346, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=32.183908045977, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=33.4146341463415, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1789941, Length=410, Percent_Identity=30.2439024390244, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082231, Length=450, Percent_Identity=22.6666666666667, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082404, Length=314, Percent_Identity=22.9299363057325, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45515; Mature: 45515

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]