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The map label for this gene is xylF [H]

Identifier: 238023826

GI number: 238023826

Start: 431269

End: 432327

Strand: Reverse

Name: xylF [H]

Synonym: bglu_2g03640

Alternate gene names: 238023826

Gene position: 432327-431269 (Counterclockwise)

Preceding gene: 238023827

Following gene: 238023825

Centisome position: 15.29

GC content: 69.88

Gene sequence:

>1059_bases
ATGAACCGATCCACCCTCACCCTCGCGCGCACCGCCGCGCTCGGTCTTGCGCTGCTCGGCGGCACGGCGGCCTTCGCGGC
GCCGGCCGGCACGGTCGCGTTCCTGATGCCCGACCAGGCCTCGACGCGCTACGAGCAGCACGACTTCCCCGGCTTCAAGG
CGCAGATGCACAAGCTCTGCCCCGACTGCAAGGTGCTGTACCAGAACGCGAACGCCGACGCCTCGCAACAGCAGCAGCAG
TTCAACTCGGTGATCGCGCAAGGCGCCAAGGTGATCGTGATCGATCCGGTCGATTCGACCGCCGCCGCCTCGCTCGTGCA
CCTGGCACGGGCGCAGGGCGTCAAGGTGATCGCCTACGACCGCCCGATCCCGTCCACGCCGGCCGACTACTACGTGTCGT
TCGACAACGAGGCGATCGGCAAGGCGATCGCGCAGTCGCTGGTGCAGCACCTGAAGGCGCTGAACGTGCCGACGACCAGG
GGCGGCGTGCTGGAAGTCAACGGCTCGCCCACCGACGCGGCGGCCGGCCTGATCCGCAACGGCATCCACGCGGGCCTGCA
GGGCAGCGGCTACGCCACGCTCGCCGAATTCGACACGCCCGAATGGGCGCCGCCGAAGGCCCAGCAATGGGTGAGCGGAC
AGATCACGCGCTTCGGCGCGAAGATCGTCGGCGTGGTGGCCGCCAACGACGGCACCGCGGGCGGCGCGATCGCGGCGTTC
AACGCGGCCGGCGTGAAGCCGCTGCCGCCCGTGACCGGCAACGACGCGACGCTCGCCGCGCTGCAGCTGATCATCGCCGG
GGACCAGTACAACACCATCCTCAAGCCAAGCGAGATCGTCGCGGCGGCCGCGGCACAGGTGGCCGTCGGCTTCCTGTCGG
GGCAGACGCCCAAGGGCGAGACGACGCTCTACCAGACGCCGACGCGGCTGTTCCAGCCGGCTCTCATCACGGCGCAGAAC
CTGAAGGCCGAAGTGGTCGACAAGGGCTTCGCGAGCGCCAAGGCGCTCTGCACCGGCCGCTACGCGCAGGGCTGCAGGCA
GCTCGGCATCACGAACTGA

Upstream 100 bases:

>100_bases
CTGGCCAACGCCATCGTTTCGGCATGCAGACAATCGACTGAACGGCGAGCGGCGCACCCAGGCCGGCCGCCACCATCAAC
CCAACGACGGAGACGAACCG

Downstream 100 bases:

>100_bases
GCGCATCCCGAGCGGCGCGCCGCGGCCACCCGCCGGCGGCCCGTCGCGGCCCATCCCTCACGGAGTCCAGACATGACGGA
GCACCCCCTCGCGCCCCGCG

Product: putative sugar ABC transporter substrate-binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNRSTLTLARTAALGLALLGGTAAFAAPAGTVAFLMPDQASTRYEQHDFPGFKAQMHKLCPDCKVLYQNANADASQQQQQ
FNSVIAQGAKVIVIDPVDSTAAASLVHLARAQGVKVIAYDRPIPSTPADYYVSFDNEAIGKAIAQSLVQHLKALNVPTTR
GGVLEVNGSPTDAAAGLIRNGIHAGLQGSGYATLAEFDTPEWAPPKAQQWVSGQITRFGAKIVGVVAANDGTAGGAIAAF
NAAGVKPLPPVTGNDATLAALQLIIAGDQYNTILKPSEIVAAAAAQVAVGFLSGQTPKGETTLYQTPTRLFQPALITAQN
LKAEVVDKGFASAKALCTGRYAQGCRQLGITN

Sequences:

>Translated_352_residues
MNRSTLTLARTAALGLALLGGTAAFAAPAGTVAFLMPDQASTRYEQHDFPGFKAQMHKLCPDCKVLYQNANADASQQQQQ
FNSVIAQGAKVIVIDPVDSTAAASLVHLARAQGVKVIAYDRPIPSTPADYYVSFDNEAIGKAIAQSLVQHLKALNVPTTR
GGVLEVNGSPTDAAAGLIRNGIHAGLQGSGYATLAEFDTPEWAPPKAQQWVSGQITRFGAKIVGVVAANDGTAGGAIAAF
NAAGVKPLPPVTGNDATLAALQLIIAGDQYNTILKPSEIVAAAAAQVAVGFLSGQTPKGETTLYQTPTRLFQPALITAQN
LKAEVVDKGFASAKALCTGRYAQGCRQLGITN
>Mature_352_residues
MNRSTLTLARTAALGLALLGGTAAFAAPAGTVAFLMPDQASTRYEQHDFPGFKAQMHKLCPDCKVLYQNANADASQQQQQ
FNSVIAQGAKVIVIDPVDSTAAASLVHLARAQGVKVIAYDRPIPSTPADYYVSFDNEAIGKAIAQSLVQHLKALNVPTTR
GGVLEVNGSPTDAAAGLIRNGIHAGLQGSGYATLAEFDTPEWAPPKAQQWVSGQITRFGAKIVGVVAANDGTAGGAIAAF
NAAGVKPLPPVTGNDATLAALQLIIAGDQYNTILKPSEIVAAAAAQVAVGFLSGQTPKGETTLYQTPTRLFQPALITAQN
LKAEVVDKGFASAKALCTGRYAQGCRQLGITN

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=282, Percent_Identity=36.1702127659575, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788473, Length=312, Percent_Identity=28.525641025641, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36466; Mature: 36466

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSTLTLARTAALGLALLGGTAAFAAPAGTVAFLMPDQASTRYEQHDFPGFKAQMHKLC
CCCCEEEHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCCCCHHCCCCCHHHHHHHHC
PDCKVLYQNANADASQQQQQFNSVIAQGAKVIVIDPVDSTAAASLVHLARAQGVKVIAYD
CCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEC
RPIPSTPADYYVSFDNEAIGKAIAQSLVQHLKALNVPTTRGGVLEVNGSPTDAAAGLIRN
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHH
GIHAGLQGSGYATLAEFDTPEWAPPKAQQWVSGQITRFGAKIVGVVAANDGTAGGAIAAF
HHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHCCCEEEEEEEECCCCCCCEEEEE
NAAGVKPLPPVTGNDATLAALQLIIAGDQYNTILKPSEIVAAAAAQVAVGFLSGQTPKGE
CCCCCCCCCCCCCCCHHHHHEEHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
TTLYQTPTRLFQPALITAQNLKAEVVDKGFASAKALCTGRYAQGCRQLGITN
EEEEECHHHHHCCHHHHHHCCHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure
MNRSTLTLARTAALGLALLGGTAAFAAPAGTVAFLMPDQASTRYEQHDFPGFKAQMHKLC
CCCCEEEHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCCCCHHCCCCCHHHHHHHHC
PDCKVLYQNANADASQQQQQFNSVIAQGAKVIVIDPVDSTAAASLVHLARAQGVKVIAYD
CCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEC
RPIPSTPADYYVSFDNEAIGKAIAQSLVQHLKALNVPTTRGGVLEVNGSPTDAAAGLIRN
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHH
GIHAGLQGSGYATLAEFDTPEWAPPKAQQWVSGQITRFGAKIVGVVAANDGTAGGAIAAF
HHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCHHHHCCCEEEEEEEECCCCCCCEEEEE
NAAGVKPLPPVTGNDATLAALQLIIAGDQYNTILKPSEIVAAAAAQVAVGFLSGQTPKGE
CCCCCCCCCCCCCCCHHHHHEEHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
TTLYQTPTRLFQPALITAQNLKAEVVDKGFASAKALCTGRYAQGCRQLGITN
EEEEECHHHHHCCHHHHHHCCHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]