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The map label for this gene is puuE [H]

Identifier: 238023802

GI number: 238023802

Start: 399954

End: 401237

Strand: Reverse

Name: puuE [H]

Synonym: bglu_2g03380

Alternate gene names: 238023802

Gene position: 401237-399954 (Counterclockwise)

Preceding gene: 238023805

Following gene: 238023801

Centisome position: 14.19

GC content: 70.09

Gene sequence:

>1284_bases
GTGAAGAATGCCGATCTGCAAGCCCGCAAGAACGCCGCCACCCCGCGCGGCGTCGGCGTGATGTGCGACTTCTATGCCGC
GCGCGCCGAGAACGCCGAGCTGTGGGACGTGGAGGGCCGCCGCTTCATCGATTTCGCGGCCGGCATCGCGGTGATGAACA
CCGGCCACCGCCACCCGAAGCTCGTCAAGGCGATCGCCGCGCAGCTCGAGCAATTCACGCACACGGCCTACCAGATCGTT
CCCTACGCGTCCTACGTCGAACTGGCCGAGAAGATCATCGCGCGCGCGCCGGGCAGCTTCGCGAAGAAGGCCGCGTTCTT
CACCACGGGCGCCGAGGCCGTCGAGAACGCCGTCAAGATGGCGCGCGCCTTCACCGGCCGCCCCGGCGTGATCGCGTTCG
CGGGCGGCTTCCACGGCCGCACCATGATGGGCATGGCGCTGACCGGCAAGGTCGCGCCGTACAAGCTCAATTTCGGGCCG
TTCCCGGGCGACGTGTTCCACGCGCCCTACCCGAACCCGCTGCACGGCGTGAGCGTGGCCGCCTCGCTGAAGGCGATCGA
CCTGCTGTTCAAGGCCGACATCGATCCGAAGCGCGTGGCGGCGATCATCTTCGAGCCGGTGCAGGGCGAAGGCGGCTTCT
ACGCGGCACCGGCCGATTTCGTGCGCGGCCTGCGCAAGATCTGCGACGAGCACGGCATCGTGCTGATCGCCGACGAGGTG
CAGACCGGCTTCGCGCGCACCGGCAAGCTGTTCGCGATGCAGCACCACGACGTGCTGCCCGACCTCATCACCATGGCCAA
GAGCCTCGCGGGCGGCATGCCGCTGTCGGGCGTGGTCGGCCGCGCCGACGTGATGGACGCGGCCGCGCCGGGCGGCCTCG
GCGGCACCTACGCGGGCAATCCGCTCGCGGTGGCCGCCGCGCACGCGGTGCTCGACATCATCGACGAGGAACGGCTCGAA
GCGCGCGCCGTCACGCTCGGCGACAAGCTCAAGGCGCGGCTCGCCGCGCTGCAGCCCGAGGTGCCGCAGATCGCCGACGT
GCGCGGCCCGGGCGCCATGGTGGCCGTCGAGTTCTTCAAGGCCGGCACGCAGGAGCCGGACGCGGACTTCACCAAGCGCG
TGCAGGCGCGCGCGCTCGAACGCGGCCTGCTGCTGCTGATCTGCGGCGTCCACTCGAACGTGATCCGCTTCCTGTTCCCG
CTGACGATCCCGGACGCCGTGTTCGACGAAGCGCTCGGCATCCTCGACACCGTGCTGAAGGAAACCGTCGGCGTCGCCGC
CTGA

Upstream 100 bases:

>100_bases
CTCTGATTTCCAGAGCCAAATTTGATTATATTCAACTCCGTCGGAGGCCCCCCGCGCCCCCCTGTATTGACCGAGTCGAA
TCGACTGGAAGGAGATATCC

Downstream 100 bases:

>100_bases
CGCGGCACCGCCCCGGCAGGCCGCCTGCCTCGCCTGCGCCGCGCCCGTCCGGGACGCGGCGTCTTGCATCGGCTTCACGG
AATCTCACATGAGCACCGCA

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKNADLQARKNAATPRGVGVMCDFYAARAENAELWDVEGRRFIDFAAGIAVMNTGHRHPKLVKAIAAQLEQFTHTAYQIV
PYASYVELAEKIIARAPGSFAKKAAFFTTGAEAVENAVKMARAFTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLNFGP
FPGDVFHAPYPNPLHGVSVAASLKAIDLLFKADIDPKRVAAIIFEPVQGEGGFYAAPADFVRGLRKICDEHGIVLIADEV
QTGFARTGKLFAMQHHDVLPDLITMAKSLAGGMPLSGVVGRADVMDAAAPGGLGGTYAGNPLAVAAAHAVLDIIDEERLE
ARAVTLGDKLKARLAALQPEVPQIADVRGPGAMVAVEFFKAGTQEPDADFTKRVQARALERGLLLLICGVHSNVIRFLFP
LTIPDAVFDEALGILDTVLKETVGVAA

Sequences:

>Translated_427_residues
MKNADLQARKNAATPRGVGVMCDFYAARAENAELWDVEGRRFIDFAAGIAVMNTGHRHPKLVKAIAAQLEQFTHTAYQIV
PYASYVELAEKIIARAPGSFAKKAAFFTTGAEAVENAVKMARAFTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLNFGP
FPGDVFHAPYPNPLHGVSVAASLKAIDLLFKADIDPKRVAAIIFEPVQGEGGFYAAPADFVRGLRKICDEHGIVLIADEV
QTGFARTGKLFAMQHHDVLPDLITMAKSLAGGMPLSGVVGRADVMDAAAPGGLGGTYAGNPLAVAAAHAVLDIIDEERLE
ARAVTLGDKLKARLAALQPEVPQIADVRGPGAMVAVEFFKAGTQEPDADFTKRVQARALERGLLLLICGVHSNVIRFLFP
LTIPDAVFDEALGILDTVLKETVGVAA
>Mature_427_residues
MKNADLQARKNAATPRGVGVMCDFYAARAENAELWDVEGRRFIDFAAGIAVMNTGHRHPKLVKAIAAQLEQFTHTAYQIV
PYASYVELAEKIIARAPGSFAKKAAFFTTGAEAVENAVKMARAFTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLNFGP
FPGDVFHAPYPNPLHGVSVAASLKAIDLLFKADIDPKRVAAIIFEPVQGEGGFYAAPADFVRGLRKICDEHGIVLIADEV
QTGFARTGKLFAMQHHDVLPDLITMAKSLAGGMPLSGVVGRADVMDAAAPGGLGGTYAGNPLAVAAAHAVLDIIDEERLE
ARAVTLGDKLKARLAALQPEVPQIADVRGPGAMVAVEFFKAGTQEPDADFTKRVQARALERGLLLLICGVHSNVIRFLFP
LTIPDAVFDEALGILDTVLKETVGVAA

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=406, Percent_Identity=33.9901477832512, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI13994255, Length=418, Percent_Identity=30.1435406698565, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI37574042, Length=420, Percent_Identity=29.7619047619048, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI284507298, Length=324, Percent_Identity=34.2592592592593, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI226442709, Length=393, Percent_Identity=30.2798982188295, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI226442705, Length=420, Percent_Identity=29.0476190476191, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI24119277, Length=414, Percent_Identity=27.0531400966184, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI188536080, Length=445, Percent_Identity=29.6629213483146, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI38679950, Length=445, Percent_Identity=29.6629213483146, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI38679946, Length=445, Percent_Identity=29.6629213483146, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=69.1211401425178, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789016, Length=421, Percent_Identity=54.6318289786223, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1789759, Length=412, Percent_Identity=34.7087378640777, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788044, Length=393, Percent_Identity=35.8778625954198, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI145693181, Length=382, Percent_Identity=35.0785340314136, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786991, Length=344, Percent_Identity=33.4302325581395, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1786349, Length=317, Percent_Identity=33.1230283911672, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25144271, Length=402, Percent_Identity=33.3333333333333, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI32564660, Length=425, Percent_Identity=31.2941176470588, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71992977, Length=422, Percent_Identity=31.7535545023697, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25144274, Length=331, Percent_Identity=31.7220543806647, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17541228, Length=430, Percent_Identity=27.6744186046512, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71981843, Length=158, Percent_Identity=32.2784810126582, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324432, Length=405, Percent_Identity=32.3456790123457, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323470, Length=398, Percent_Identity=32.9145728643216, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321456, Length=359, Percent_Identity=28.9693593314763, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324386, Length=331, Percent_Identity=24.773413897281, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574759, Length=453, Percent_Identity=30.6843267108168, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI161085790, Length=448, Percent_Identity=30.1339285714286, Blast_Score=198, Evalue=6e-51,
Organism=Drosophila melanogaster, GI21357415, Length=397, Percent_Identity=32.9974811083123, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21356575, Length=404, Percent_Identity=30.6930693069307, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281366494, Length=363, Percent_Identity=27.8236914600551, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24667139, Length=363, Percent_Identity=27.8236914600551, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24667143, Length=363, Percent_Identity=27.8236914600551, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 45325; Mature: 45325

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00600 AA_TRANSFER_CLASS_3 ; PS00636 DNAJ_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNADLQARKNAATPRGVGVMCDFYAARAENAELWDVEGRRFIDFAAGIAVMNTGHRHPK
CCCCCCCHHHCCCCCCCCCCEEHHHHHCCCCCEEEECCCCEEHHHHHCHHHCCCCCCCHH
LVKAIAAQLEQFTHTAYQIVPYASYVELAEKIIARAPGSFAKKAAFFTTGAEAVENAVKM
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
ARAFTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLNFGPFPGDVFHAPYPNPLHGVSVA
HHHHCCCCCEEEEECCCCCCEEECEEEECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHH
ASLKAIDLLFKADIDPKRVAAIIFEPVQGEGGFYAAPADFVRGLRKICDEHGIVLIADEV
HHHHHHHHHHHCCCCHHHEEEEEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCH
QTGFARTGKLFAMQHHDVLPDLITMAKSLAGGMPLSGVVGRADVMDAAAPGGLGGTYAGN
HCCHHHCCCEEEEHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
PLAVAAAHAVLDIIDEERLEARAVTLGDKLKARLAALQPEVPQIADVRGPGAMVAVEFFK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHEEEHHHH
AGTQEPDADFTKRVQARALERGLLLLICGVHSNVIRFLFPLTIPDAVFDEALGILDTVLK
CCCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ETVGVAA
HHHCCCC
>Mature Secondary Structure
MKNADLQARKNAATPRGVGVMCDFYAARAENAELWDVEGRRFIDFAAGIAVMNTGHRHPK
CCCCCCCHHHCCCCCCCCCCEEHHHHHCCCCCEEEECCCCEEHHHHHCHHHCCCCCCCHH
LVKAIAAQLEQFTHTAYQIVPYASYVELAEKIIARAPGSFAKKAAFFTTGAEAVENAVKM
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
ARAFTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLNFGPFPGDVFHAPYPNPLHGVSVA
HHHHCCCCCEEEEECCCCCCEEECEEEECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHH
ASLKAIDLLFKADIDPKRVAAIIFEPVQGEGGFYAAPADFVRGLRKICDEHGIVLIADEV
HHHHHHHHHHHCCCCHHHEEEEEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCH
QTGFARTGKLFAMQHHDVLPDLITMAKSLAGGMPLSGVVGRADVMDAAAPGGLGGTYAGN
HCCHHHCCCEEEEHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
PLAVAAAHAVLDIIDEERLEARAVTLGDKLKARLAALQPEVPQIADVRGPGAMVAVEFFK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHEEEHHHH
AGTQEPDADFTKRVQARALERGLLLLICGVHSNVIRFLFPLTIPDAVFDEALGILDTVLK
CCCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ETVGVAA
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]