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The map label for this gene is rutF [H]

Identifier: 238023748

GI number: 238023748

Start: 326375

End: 327007

Strand: Direct

Name: rutF [H]

Synonym: bglu_2g02800

Alternate gene names: 238023748

Gene position: 326375-327007 (Clockwise)

Preceding gene: 238023747

Following gene: 238023749

Centisome position: 11.54

GC content: 76.3

Gene sequence:

>633_bases
ATGGCCAGCCCATGGAGCAACGCAACAATCGACGGCGCGCCGGGCGCCGGACTCGAGGCGGCCGCGCCGGCGCCGGGGCA
GGCGCGGCCCGCCGCGCCGGGCGCACCGTCCGCCGCCGCGCCGGAGGCGCATGACGGGCGCGGCGGCGCCGAACTCGCGC
GCCAGTTCCGGCACGCGATGCGCCGGCTCACTGCCGCCGTGTCGATCGTGGCGACACGCGAGCACGGCGCGCGCTACGGC
ATGGCGGCCACCGCGCTCAGCACGCTCTGCACCGAGCCGCCCGCGATCGTGGTCTGCATCAATCGCAACGCGAGCGTCTA
CCTGCCGCTCGCGCGCACGCGGCGCTTCTCGGTCAATCTGCTGCAGGCGCGCCAGGCCGACCTGATCGCGCCGTTCAGCG
GCAAGCTCGAGCACGCGGCGCGCTTCGCGTTCGGCGCCTGGCGCGAGGCGCGCGGGCTGCCGGTGCTGGGCGGCGCGCAG
GCCACGCTGCTGTGCCGGGTCGACGCGATGTACGGCTACGGCAGCCACGATCTCGTGATCGGGCGCGTCGAGGCGGTGAC
GGCCGCCGAATCGGTCACGCCGCTGTTGTGGCAGAACGGCGCGCCGGCCGCCGCGAGCGCGCTCGGCGCATGA

Upstream 100 bases:

>100_bases
CTGCTGCTGCTGATGTTCTCCGGCACGCTGATGTACTCGGTGCTCAAGAGCTGAGCGGCGCGCCGCCGCTTCCAACCAAC
CGACATGACGAGGAGACGTG

Downstream 100 bases:

>100_bases
GCACGGCGCGCCGCTCGCCTGGCCGTGCTACGGCGGGCCTTCGCACGCAGGCCCGCGCAGCCAGGCCGGCAGCCCGGGCG
CGGCGCCAGACGGCCGCCTG

Product: Flavin reductase domain-containing protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MASPWSNATIDGAPGAGLEAAAPAPGQARPAAPGAPSAAAPEAHDGRGGAELARQFRHAMRRLTAAVSIVATREHGARYG
MAATALSTLCTEPPAIVVCINRNASVYLPLARTRRFSVNLLQARQADLIAPFSGKLEHAARFAFGAWREARGLPVLGGAQ
ATLLCRVDAMYGYGSHDLVIGRVEAVTAAESVTPLLWQNGAPAAASALGA

Sequences:

>Translated_210_residues
MASPWSNATIDGAPGAGLEAAAPAPGQARPAAPGAPSAAAPEAHDGRGGAELARQFRHAMRRLTAAVSIVATREHGARYG
MAATALSTLCTEPPAIVVCINRNASVYLPLARTRRFSVNLLQARQADLIAPFSGKLEHAARFAFGAWREARGLPVLGGAQ
ATLLCRVDAMYGYGSHDLVIGRVEAVTAAESVTPLLWQNGAPAAASALGA
>Mature_209_residues
ASPWSNATIDGAPGAGLEAAAPAPGQARPAAPGAPSAAAPEAHDGRGGAELARQFRHAMRRLTAAVSIVATREHGARYGM
AATALSTLCTEPPAIVVCINRNASVYLPLARTRRFSVNLLQARQADLIAPFSGKLEHAARFAFGAWREARGLPVLGGAQA
TLLCRVDAMYGYGSHDLVIGRVEAVTAAESVTPLLWQNGAPAAASALGA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=145, Percent_Identity=33.7931034482759, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 21570; Mature: 21439

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPWSNATIDGAPGAGLEAAAPAPGQARPAAPGAPSAAAPEAHDGRGGAELARQFRHAM
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RRLTAAVSIVATREHGARYGMAATALSTLCTEPPAIVVCINRNASVYLPLARTRRFSVNL
HHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHCCEEEHHH
LQARQADLIAPFSGKLEHAARFAFGAWREARGLPVLGGAQATLLCRVDAMYGYGSHDLVI
HHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEEHHCCCCCCCEEE
GRVEAVTAAESVTPLLWQNGAPAAASALGA
EEHHHHHHHHHCCHHEECCCCCHHHHHCCC
>Mature Secondary Structure 
ASPWSNATIDGAPGAGLEAAAPAPGQARPAAPGAPSAAAPEAHDGRGGAELARQFRHAM
CCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RRLTAAVSIVATREHGARYGMAATALSTLCTEPPAIVVCINRNASVYLPLARTRRFSVNL
HHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHCCEEEHHH
LQARQADLIAPFSGKLEHAARFAFGAWREARGLPVLGGAQATLLCRVDAMYGYGSHDLVI
HHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEEHHCCCCCCCEEE
GRVEAVTAAESVTPLLWQNGAPAAASALGA
EEHHHHHHHHHCCHHEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA