Click here to switch to the map view.

The map label for this gene is grsT [H]

Identifier: 238023740

GI number: 238023740

Start: 319966

End: 320754

Strand: Direct

Name: grsT [H]

Synonym: bglu_2g02720

Alternate gene names: 238023740

Gene position: 319966-320754 (Clockwise)

Preceding gene: 238023739

Following gene: 238023741

Centisome position: 11.32

GC content: 73.38

Gene sequence:

>789_bases
ATGACCGGCGCCGGCCTGCAGACGCGCGCATCCGACTGGCTGCTGTTCGGCGCCGGCGGTCCGAGGTCGGACGCGCGCGT
GCGGCTCGTCTGCTTCCATTACGCGGGCAGCGGCGCGTCGATGTTCGCGCCCTGGGCCGCGGCGCTGCCCGACGCGGTGG
AGCTGCTCGCCGTGCAGCTGCCGGGCCGCGAGAACCGCCTGGACGAGCCGCTGCTGCATGCCATGGACGAGATCGGCTCG
CCGGTGGCCGAGGCGCTCGCGCCCTGGCTCGACCGGCCCTACCTGATGTTCGGCCACAGCACCGGCGCGCGCATCGCGTT
CGACGTGGCGCGGCGGCTGCGCCGCGCCGGCCTGGCGCTGCCGCGCGCGCTGATCGTCTCGGCGCAATACGCCCCCGACG
TGAGGCCCGCGGTGATGCGCTACGCGCTCGACGACGCGCAGTTCGTGGAGGTATTGCGCGCCTGCGAGGGCACGCCCGAC
ACGATCCTCGACGATCCGGTGCTGCTCGACCTGCTGCTGCCCAGGATCCGCGCCGACGGCGAGGTGTTCGAGACCTACCG
CTACCAGGCCGAGCCGCCGCTCGACTGCCCGATCGTGGTGTTCCAGGGCACCGCCGACGCGATGGTCGACGATGCCGGGG
CCGCGGGCTGGGCGCGCCATACGCGCGGCGGCTTTCGGCTGCGCCGCTTCGAGGGCGGCCACTTCTTCATTCACGAGAAG
GAGGCCGAGGTGCTCGACGAGATCAACCGGGAGCTGGCCGCGCTGCTCGGCGAGCCGGACTCTCCCTGA

Upstream 100 bases:

>100_bases
CGATCGCTACTCGACGGTGCTGGTGTCCGGCGAGGATCGCTACGGCCACAACCGCGTCACGCTGCCACTGGCCGCCAAGG
CCGCGGCGACGGCCTGAGCG

Downstream 100 bases:

>100_bases
CTGGATGACAAGACGATGGCCCAATGGAATACGGATGAGGAACTGTTCGCGCTGATGCGCGAGGAACTGGCGACCTGCCC
CGTCAGCGACGTGCTCGAGC

Product: Non-ribosomal peptide synthesis thioesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTGAGLQTRASDWLLFGAGGPRSDARVRLVCFHYAGSGASMFAPWAAALPDAVELLAVQLPGRENRLDEPLLHAMDEIGS
PVAEALAPWLDRPYLMFGHSTGARIAFDVARRLRRAGLALPRALIVSAQYAPDVRPAVMRYALDDAQFVEVLRACEGTPD
TILDDPVLLDLLLPRIRADGEVFETYRYQAEPPLDCPIVVFQGTADAMVDDAGAAGWARHTRGGFRLRRFEGGHFFIHEK
EAEVLDEINRELAALLGEPDSP

Sequences:

>Translated_262_residues
MTGAGLQTRASDWLLFGAGGPRSDARVRLVCFHYAGSGASMFAPWAAALPDAVELLAVQLPGRENRLDEPLLHAMDEIGS
PVAEALAPWLDRPYLMFGHSTGARIAFDVARRLRRAGLALPRALIVSAQYAPDVRPAVMRYALDDAQFVEVLRACEGTPD
TILDDPVLLDLLLPRIRADGEVFETYRYQAEPPLDCPIVVFQGTADAMVDDAGAAGWARHTRGGFRLRRFEGGHFFIHEK
EAEVLDEINRELAALLGEPDSP
>Mature_261_residues
TGAGLQTRASDWLLFGAGGPRSDARVRLVCFHYAGSGASMFAPWAAALPDAVELLAVQLPGRENRLDEPLLHAMDEIGSP
VAEALAPWLDRPYLMFGHSTGARIAFDVARRLRRAGLALPRALIVSAQYAPDVRPAVMRYALDDAQFVEVLRACEGTPDT
ILDDPVLLDLLLPRIRADGEVFETYRYQAEPPLDCPIVVFQGTADAMVDDAGAAGWARHTRGGFRLRRFEGGHFFIHEKE
AEVLDEINRELAALLGEPDSP

Specific function: Probable thioesterase involved in the biosynthesis of gramicidin S [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=236, Percent_Identity=29.6610169491525, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI8922871, Length=297, Percent_Identity=24.2424242424242, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 28659; Mature: 28528

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAGLQTRASDWLLFGAGGPRSDARVRLVCFHYAGSGASMFAPWAAALPDAVELLAVQL
CCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCHHCCHHHHCHHHHHHHHEEC
PGRENRLDEPLLHAMDEIGSPVAEALAPWLDRPYLMFGHSTGARIAFDVARRLRRAGLAL
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
PRALIVSAQYAPDVRPAVMRYALDDAQFVEVLRACEGTPDTILDDPVLLDLLLPRIRADG
CHHHHEECCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
EVFETYRYQAEPPLDCPIVVFQGTADAMVDDAGAAGWARHTRGGFRLRRFEGGHFFIHEK
HHHHHHCCCCCCCCCCCEEEECCCHHHHHCCCCCCCHHHHCCCCCEEEEECCCEEEEECC
EAEVLDEINRELAALLGEPDSP
HHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TGAGLQTRASDWLLFGAGGPRSDARVRLVCFHYAGSGASMFAPWAAALPDAVELLAVQL
CCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCCCCHHCCHHHHCHHHHHHHHEEC
PGRENRLDEPLLHAMDEIGSPVAEALAPWLDRPYLMFGHSTGARIAFDVARRLRRAGLAL
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
PRALIVSAQYAPDVRPAVMRYALDDAQFVEVLRACEGTPDTILDDPVLLDLLLPRIRADG
CHHHHEECCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
EVFETYRYQAEPPLDCPIVVFQGTADAMVDDAGAAGWARHTRGGFRLRRFEGGHFFIHEK
HHHHHHCCCCCCCCCCCEEEECCCHHHHHCCCCCCCHHHHCCCCCEEEEECCCEEEEECC
EAEVLDEINRELAALLGEPDSP
HHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2477357; 7512553 [H]