Click here to switch to the map view.

The map label for this gene is mycB [H]

Identifier: 238023737

GI number: 238023737

Start: 317073

End: 318662

Strand: Direct

Name: mycB [H]

Synonym: bglu_2g02690

Alternate gene names: 238023737

Gene position: 317073-318662 (Clockwise)

Preceding gene: 238023736

Following gene: 238023738

Centisome position: 11.21

GC content: 69.06

Gene sequence:

>1590_bases
ATGTCCGGACACATCGTTTACGCCATCCAGACACACGCGCAGGCCACGCCGTATCGCGCGGCGCTGTCCGATTCACGCGG
CGATTTCAGCTATGCCGACCTCGACATCTTCAGCACGCGTTTCGCGCTGCGCCTGCAGTCGTATGGCTGCGTGCCGGGCG
ACCGCGTGGTGATGGTGGCGAGCCGCTCGGCGCTGATGCTCGCATGCGTGATCGGCACCTTCAAGGCCGGCTGCATCCAC
GTGCCGCTCGACCCGAAGATGCCGGCCGAGCGGCTGCGCTACATCCTCGCCGACATCGCGCCGGCGCTCGTGATCGCCGA
CGGCGAGCTAGCCGAACGGGTTCGCGAGGCGCTGCCCGAGGCGGCGCCGGTGCTGCGCGTGAGCGACGTCGAGCGGCTTC
GCGACGATGACGACTCGGCGCTGCGCGATGCCTGCGTGCGCGCGCTGCCGCTGCCCGATCTGGATGCGTCGGCCACCGCC
TACTGCATCTACACCTCGGGCTCGACCGGCCGGCCCAAGGGCGTGCTGATCGCCCATCGCGCCATCATCGACTTCTTCGA
CGGCACGCGCCGCGTCTACGAGGTGACGGCGCAGTCGCGCTGCGCGAGCTTCTCGCCGCTGCACTTCGACGTGTTCCTGA
TGGACATGCTGTTTCCGCTCGCACAGGGCGCGCACCTGTACGTCCACGACGACGTGGTGGTGCCCGACATCATGCTCGAC
GCGCTCTGCGCGCACGAGGTCACGCACTTTTCCGCGTGGGGCATGATGCTGGGCCTGCTGGCGCAGGCCGAGCGCTTCGG
CAGCGCGCCGCTGCCGCATCTGAAGCAGATCCTGACCGGCACCGACGTGCCCGACATCAAGACGGTCCAGCGCTGGATGC
GCAAGACGCCCGGCGTGCAGGTGATCAACGCCTACGGGCCGACCGAGGCGACCTGCGCGGCCACCGCGCACGTGATCCGC
GAGATCGAGCCGGAGCGCCGCACGCTCTACCCGATCGGCGTGCCGCTTGAGCACGTGAAGGTGCTGCTGGTGGGCGACGA
CGGCCGTGCGATCGACACCCCCGACACGCCCGGCGAGCTGATGGTGGGCGGCACCCAGGTGATGCAGGGCTACTGGCACC
TGCCCGAGGAGACGGCCGCGCGGCTCACGCAGGTGGGCGGCGTGCCGTTCTATCGCACCGGCGACCTGTGCCGCTGGCTC
GACGACGGCAGCCTGTTCTACATGGGCCGCCGCGACAACGAGGTCAAGCTCGGCGGCTACCGGATCCATCTGAACGAGGT
GCGGCGCGTGATCGACAGCGTGCCGCAGGTTCACGCCTCGGAGGTCGTGGTGCTCGACACGCGCATCGGCGAGCCGGTGC
TCGCAGCCGCGGTGCTGTTCGAGCAGGCGCACGCGGCCGACACCGAGCCGCGGCTCGCCGCGATCCGGCGGCGGCTCGCG
GGCGAGCTACCGGCCTACATGGTGCCGCGCTATCTGACGGCGCTGGACGAGTTTCCGATGCTGTCGTCAGGGAAGACGGA
CCGCAAGGCGCTGCTGTCGATTTTGCAGCAACGCATCAACACAAGCCACCAGGAGGAAGTCAATTCATGA

Upstream 100 bases:

>100_bases
TGCCCGCAGGCACGACGCGACGTGCCCGCGGGACCGGTGCGCGATGCCGCCCGCCTTGCAGAAGGTGCCCCTGGAGACAC
ATTGCAGGAGGGTCACCGAG

Downstream 100 bases:

>100_bases
AAACCGAAGCCATCTTGATACAGGTTCTGGAAGACGTGATCGGCGTGAAGAACGTGACGCCCGACACGCGCTTTCCGGAT
ATCGGCGGCAACTCGCTGAA

Product: amino acid adenylation domain-containing protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent tyrosine adenylase; TyrA; Tyrosine activase; ATP-dependent asparagine adenylase 2; AsnA 2; Asparagine activase 2; ATP-dependent glutamine adenylase; GlnA; Glutamine activase; ATP-dependent proline adenylase; ProA; Proline activase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSGHIVYAIQTHAQATPYRAALSDSRGDFSYADLDIFSTRFALRLQSYGCVPGDRVVMVASRSALMLACVIGTFKAGCIH
VPLDPKMPAERLRYILADIAPALVIADGELAERVREALPEAAPVLRVSDVERLRDDDDSALRDACVRALPLPDLDASATA
YCIYTSGSTGRPKGVLIAHRAIIDFFDGTRRVYEVTAQSRCASFSPLHFDVFLMDMLFPLAQGAHLYVHDDVVVPDIMLD
ALCAHEVTHFSAWGMMLGLLAQAERFGSAPLPHLKQILTGTDVPDIKTVQRWMRKTPGVQVINAYGPTEATCAATAHVIR
EIEPERRTLYPIGVPLEHVKVLLVGDDGRAIDTPDTPGELMVGGTQVMQGYWHLPEETAARLTQVGGVPFYRTGDLCRWL
DDGSLFYMGRRDNEVKLGGYRIHLNEVRRVIDSVPQVHASEVVVLDTRIGEPVLAAAVLFEQAHAADTEPRLAAIRRRLA
GELPAYMVPRYLTALDEFPMLSSGKTDRKALLSILQQRINTSHQEEVNS

Sequences:

>Translated_529_residues
MSGHIVYAIQTHAQATPYRAALSDSRGDFSYADLDIFSTRFALRLQSYGCVPGDRVVMVASRSALMLACVIGTFKAGCIH
VPLDPKMPAERLRYILADIAPALVIADGELAERVREALPEAAPVLRVSDVERLRDDDDSALRDACVRALPLPDLDASATA
YCIYTSGSTGRPKGVLIAHRAIIDFFDGTRRVYEVTAQSRCASFSPLHFDVFLMDMLFPLAQGAHLYVHDDVVVPDIMLD
ALCAHEVTHFSAWGMMLGLLAQAERFGSAPLPHLKQILTGTDVPDIKTVQRWMRKTPGVQVINAYGPTEATCAATAHVIR
EIEPERRTLYPIGVPLEHVKVLLVGDDGRAIDTPDTPGELMVGGTQVMQGYWHLPEETAARLTQVGGVPFYRTGDLCRWL
DDGSLFYMGRRDNEVKLGGYRIHLNEVRRVIDSVPQVHASEVVVLDTRIGEPVLAAAVLFEQAHAADTEPRLAAIRRRLA
GELPAYMVPRYLTALDEFPMLSSGKTDRKALLSILQQRINTSHQEEVNS
>Mature_528_residues
SGHIVYAIQTHAQATPYRAALSDSRGDFSYADLDIFSTRFALRLQSYGCVPGDRVVMVASRSALMLACVIGTFKAGCIHV
PLDPKMPAERLRYILADIAPALVIADGELAERVREALPEAAPVLRVSDVERLRDDDDSALRDACVRALPLPDLDASATAY
CIYTSGSTGRPKGVLIAHRAIIDFFDGTRRVYEVTAQSRCASFSPLHFDVFLMDMLFPLAQGAHLYVHDDVVVPDIMLDA
LCAHEVTHFSAWGMMLGLLAQAERFGSAPLPHLKQILTGTDVPDIKTVQRWMRKTPGVQVINAYGPTEATCAATAHVIRE
IEPERRTLYPIGVPLEHVKVLLVGDDGRAIDTPDTPGELMVGGTQVMQGYWHLPEETAARLTQVGGVPFYRTGDLCRWLD
DGSLFYMGRRDNEVKLGGYRIHLNEVRRVIDSVPQVHASEVVVLDTRIGEPVLAAAVLFEQAHAADTEPRLAAIRRRLAG
ELPAYMVPRYLTALDEFPMLSSGKTDRKALLSILQQRINTSHQEEVNS

Specific function: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Tyr, Asn, Gln and Pro as part of the synthesis of mycosubtilin. The Asn and Gln residues are further epimerized to the D-isomer form. The activation sites for these

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=547, Percent_Identity=26.3254113345521, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI187761343, Length=547, Percent_Identity=26.3254113345521, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI156151445, Length=550, Percent_Identity=24, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI45580730, Length=385, Percent_Identity=25.974025974026, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=24.9027237354086, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI38505220, Length=492, Percent_Identity=25.4065040650407, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI42544132, Length=502, Percent_Identity=23.7051792828685, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI58082049, Length=483, Percent_Identity=23.3954451345756, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157311624, Length=492, Percent_Identity=22.9674796747967, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI157311622, Length=492, Percent_Identity=22.9674796747967, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1786801, Length=529, Percent_Identity=30.6238185255198, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI145693145, Length=549, Percent_Identity=26.2295081967213, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788107, Length=523, Percent_Identity=24.4741873804971, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789201, Length=417, Percent_Identity=26.378896882494, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786810, Length=525, Percent_Identity=24.7619047619048, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=21.6796875, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=501, Percent_Identity=22.3552894211577, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=529, Percent_Identity=24.1965973534972, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=529, Percent_Identity=24.1965973534972, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=529, Percent_Identity=24.1965973534972, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556356, Length=373, Percent_Identity=26.2734584450402, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319591, Length=598, Percent_Identity=24.9163879598662, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319699, Length=489, Percent_Identity=22.4948875255624, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648676, Length=499, Percent_Identity=27.2545090180361, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24581924, Length=523, Percent_Identity=23.9005736137667, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922652, Length=523, Percent_Identity=24.282982791587, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648260, Length=519, Percent_Identity=21.9653179190751, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18859661, Length=414, Percent_Identity=26.0869565217391, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24582852, Length=387, Percent_Identity=23.5142118863049, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653035, Length=490, Percent_Identity=22.8571428571429, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356441, Length=415, Percent_Identity=21.4457831325301, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648253, Length=522, Percent_Identity=22.9885057471264, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356947, Length=504, Percent_Identity=21.6269841269841, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076582, Length=496, Percent_Identity=21.5725806451613, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281365686, Length=508, Percent_Identity=21.4566929133858, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 58274; Mature: 58142

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGHIVYAIQTHAQATPYRAALSDSRGDFSYADLDIFSTRFALRLQSYGCVPGDRVVMVA
CCCCEEEEEECCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEE
SRSALMLACVIGTFKAGCIHVPLDPKMPAERLRYILADIAPALVIADGELAERVREALPE
CCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCC
AAPVLRVSDVERLRDDDDSALRDACVRALPLPDLDASATAYCIYTSGSTGRPKGVLIAHR
CCCEEEHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHH
AIIDFFDGTRRVYEVTAQSRCASFSPLHFDVFLMDMLFPLAQGAHLYVHDDVVVPDIMLD
HHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCEECHHHHHH
ALCAHEVTHFSAWGMMLGLLAQAERFGSAPLPHLKQILTGTDVPDIKTVQRWMRKTPGVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEE
VINAYGPTEATCAATAHVIREIEPERRTLYPIGVPLEHVKVLLVGDDGRAIDTPDTPGEL
EEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHEEEEEECCCCCEECCCCCCCCE
MVGGTQVMQGYWHLPEETAARLTQVGGVPFYRTGDLCRWLDDGSLFYMGRRDNEVKLGGY
EECHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCHHHEECCCCEEEEECCCCEEEECEE
RIHLNEVRRVIDSVPQVHASEVVVLDTRIGEPVLAAAVLFEQAHAADTEPRLAAIRRRLA
EEEHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GELPAYMVPRYLTALDEFPMLSSGKTDRKALLSILQQRINTSHQEEVNS
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
SGHIVYAIQTHAQATPYRAALSDSRGDFSYADLDIFSTRFALRLQSYGCVPGDRVVMVA
CCCEEEEEECCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEE
SRSALMLACVIGTFKAGCIHVPLDPKMPAERLRYILADIAPALVIADGELAERVREALPE
CCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCC
AAPVLRVSDVERLRDDDDSALRDACVRALPLPDLDASATAYCIYTSGSTGRPKGVLIAHR
CCCEEEHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHH
AIIDFFDGTRRVYEVTAQSRCASFSPLHFDVFLMDMLFPLAQGAHLYVHDDVVVPDIMLD
HHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCEECHHHHHH
ALCAHEVTHFSAWGMMLGLLAQAERFGSAPLPHLKQILTGTDVPDIKTVQRWMRKTPGVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCEE
VINAYGPTEATCAATAHVIREIEPERRTLYPIGVPLEHVKVLLVGDDGRAIDTPDTPGEL
EEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHEEEEEECCCCCEECCCCCCCCE
MVGGTQVMQGYWHLPEETAARLTQVGGVPFYRTGDLCRWLDDGSLFYMGRRDNEVKLGGY
EECHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCHHHEECCCCEEEEECCCCEEEECEE
RIHLNEVRRVIDSVPQVHASEVVVLDTRIGEPVLAAAVLFEQAHAADTEPRLAAIRRRLA
EEEHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GELPAYMVPRYLTALDEFPMLSSGKTDRKALLSILQQRINTSHQEEVNS
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]