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The map label for this gene is gatA [H]

Identifier: 238023592

GI number: 238023592

Start: 100025

End: 101158

Strand: Reverse

Name: gatA [H]

Synonym: bglu_2g00950

Alternate gene names: 238023592

Gene position: 101158-100025 (Counterclockwise)

Preceding gene: 238023593

Following gene: 238023591

Centisome position: 3.58

GC content: 75.13

Gene sequence:

>1134_bases
ATGAGCACCTTCATCCAGACCCTGGCGCTCGGCGGCCGCGGCCCCGACAGCCCGACGATCGCCATCAAGGACACGATCGA
CATCGCCGGCCACCGAACCACCGGCGCGAGCCGCGCCCTCGCCGAGGTCGCGCCGGCGCGCCGCCACGCGCACGTGGTGC
AGCGCCTGCTCGACGCCGGCTGGCGCATCGTCGGCAAGGCCAACATGCACGAGCTGGCATTCGGCATGACCGGCATCAAC
GACTACACCGGCACGCCCGAGAACCCGCAGGCGCGCACGCGCATTCCCGGCGGTTCGTCGAGCGGCTCGGCTGCCGCGGT
CGGGCTGGGACTCGCCGACGCCGCGCTCGGCACCGACACCGGCGGCTCGATCCGCGGCCCGGCGGCCTGCTGCGGCGTGA
TCGGGCTGAAGCCGACGTTCGGGCGCGTCTCGCGCGACGGCGTGGCCCCGGCCGTCACCACGCTCGACTGCGTCGGCCCG
TTCGCGCGCGAGATGCGCATGCTGATCGCCGCGATGACGGCGATCAGCGCCGATTTCGACGGCCGGGCGGCCGCCGCGCC
GGCCGCCGCGCTGCGGATCGGCATCGTCGCGGCGCCGGCTCGGCACGAGTTCGGCGCCGCCGTCGTCGACGCGGTCCGGC
GCGCCGGCAACGGCGCTCGGGCGTGCGCGCTGCCGCGCCTGGGCGAGGCCTTCGAAGCCGGCCTCGCCGTGATCAATGCC
GAAACCTCGGCGGCCTTCGGCCATCTGGTGGGCAGCGGCAAGCTGGGCGCCGATCTCGAGGCACGCCTGCGCGCCGCGGC
GGCCACGACGCGCGAGGCGCTCGAGGCCGCCGAGCGGGTGCGGCGCGCCTTCACCGACGAGGTGGATCGCGCGCTCGAAC
GGGTCGACCTGCTGGTCATGCCGACCCTGCCGGACTTCCCGATCACCGTGGAGGAGGCGCGCGGCGGCCGCCCGGTCATC
GCGATGTCGTCGCTGATCCGCCCGTTCAATCTCAGCGGTCATCCGGCGCTGAGCCTGCCGCTGCCGGTCGCCGGCTCGAC
GCTGAAGGCCGGCCTGCAGATCGTCGGCCGCCACGACGCGGACGAGCAGGTATGCGCGTTCGGCTCGCACCTGGAGCGCG
CGCTGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCTGGCGAATCGCGCATTTGCGCACCGAACGCCTGTTCGACGCGCCGTGGCAGGTCAATCCGCGCGCGGCACTGCCGCCC
GCCGACGGGAGCACGCCCGC

Downstream 100 bases:

>100_bases
GCCCGGCGCCGGCGAGGCCGCGCCGCCAGCCGGCGCAGCGCGCCGGCTCGTTCGCTGCTCATACGAAATCGCGCGCGCCG
ACGCGCCCGCGCACGGTGGC

Product: Pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSTFIQTLALGGRGPDSPTIAIKDTIDIAGHRTTGASRALAEVAPARRHAHVVQRLLDAGWRIVGKANMHELAFGMTGIN
DYTGTPENPQARTRIPGGSSSGSAAAVGLGLADAALGTDTGGSIRGPAACCGVIGLKPTFGRVSRDGVAPAVTTLDCVGP
FAREMRMLIAAMTAISADFDGRAAAAPAAALRIGIVAAPARHEFGAAVVDAVRRAGNGARACALPRLGEAFEAGLAVINA
ETSAAFGHLVGSGKLGADLEARLRAAAATTREALEAAERVRRAFTDEVDRALERVDLLVMPTLPDFPITVEEARGGRPVI
AMSSLIRPFNLSGHPALSLPLPVAGSTLKAGLQIVGRHDADEQVCAFGSHLERALAA

Sequences:

>Translated_377_residues
MSTFIQTLALGGRGPDSPTIAIKDTIDIAGHRTTGASRALAEVAPARRHAHVVQRLLDAGWRIVGKANMHELAFGMTGIN
DYTGTPENPQARTRIPGGSSSGSAAAVGLGLADAALGTDTGGSIRGPAACCGVIGLKPTFGRVSRDGVAPAVTTLDCVGP
FAREMRMLIAAMTAISADFDGRAAAAPAAALRIGIVAAPARHEFGAAVVDAVRRAGNGARACALPRLGEAFEAGLAVINA
ETSAAFGHLVGSGKLGADLEARLRAAAATTREALEAAERVRRAFTDEVDRALERVDLLVMPTLPDFPITVEEARGGRPVI
AMSSLIRPFNLSGHPALSLPLPVAGSTLKAGLQIVGRHDADEQVCAFGSHLERALAA
>Mature_376_residues
STFIQTLALGGRGPDSPTIAIKDTIDIAGHRTTGASRALAEVAPARRHAHVVQRLLDAGWRIVGKANMHELAFGMTGIND
YTGTPENPQARTRIPGGSSSGSAAAVGLGLADAALGTDTGGSIRGPAACCGVIGLKPTFGRVSRDGVAPAVTTLDCVGPF
AREMRMLIAAMTAISADFDGRAAAAPAAALRIGIVAAPARHEFGAAVVDAVRRAGNGARACALPRLGEAFEAGLAVINAE
TSAAFGHLVGSGKLGADLEARLRAAAATTREALEAAERVRRAFTDEVDRALERVDLLVMPTLPDFPITVEEARGGRPVIA
MSSLIRPFNLSGHPALSLPLPVAGSTLKAGLQIVGRHDADEQVCAFGSHLERALAA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=430, Percent_Identity=26.046511627907, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=403, Percent_Identity=30.5210918114144, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17537465, Length=378, Percent_Identity=24.0740740740741, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=415, Percent_Identity=26.7469879518072, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319685, Length=143, Percent_Identity=45.4545454545455, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648113, Length=412, Percent_Identity=31.3106796116505, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356731, Length=130, Percent_Identity=35.3846153846154, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648435, Length=162, Percent_Identity=31.4814814814815, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648437, Length=162, Percent_Identity=31.4814814814815, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550774, Length=162, Percent_Identity=31.4814814814815, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648441, Length=162, Percent_Identity=31.4814814814815, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648439, Length=162, Percent_Identity=31.4814814814815, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652985, Length=358, Percent_Identity=26.2569832402235, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922090, Length=358, Percent_Identity=26.2569832402235, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24652981, Length=358, Percent_Identity=26.2569832402235, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24652983, Length=358, Percent_Identity=26.2569832402235, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 38643; Mature: 38511

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFIQTLALGGRGPDSPTIAIKDTIDIAGHRTTGASRALAEVAPARRHAHVVQRLLDAG
CCHHHHHHHCCCCCCCCCEEEEEECEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
WRIVGKANMHELAFGMTGINDYTGTPENPQARTRIPGGSSSGSAAAVGLGLADAALGTDT
CEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEECCHHHHHCCCCC
GGSIRGPAACCGVIGLKPTFGRVSRDGVAPAVTTLDCVGPFAREMRMLIAAMTAISADFD
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GRAAAAPAAALRIGIVAAPARHEFGAAVVDAVRRAGNGARACALPRLGEAFEAGLAVINA
CCCHHCCHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHCEEEEEC
ETSAAFGHLVGSGKLGADLEARLRAAAATTREALEAAERVRRAFTDEVDRALERVDLLVM
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
PTLPDFPITVEEARGGRPVIAMSSLIRPFNLSGHPALSLPLPVAGSTLKAGLQIVGRHDA
CCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHCCCCCC
DEQVCAFGSHLERALAA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STFIQTLALGGRGPDSPTIAIKDTIDIAGHRTTGASRALAEVAPARRHAHVVQRLLDAG
CHHHHHHHCCCCCCCCCEEEEEECEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
WRIVGKANMHELAFGMTGINDYTGTPENPQARTRIPGGSSSGSAAAVGLGLADAALGTDT
CEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCEEEECCHHHHHCCCCC
GGSIRGPAACCGVIGLKPTFGRVSRDGVAPAVTTLDCVGPFAREMRMLIAAMTAISADFD
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GRAAAAPAAALRIGIVAAPARHEFGAAVVDAVRRAGNGARACALPRLGEAFEAGLAVINA
CCCHHCCHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHCEEEEEC
ETSAAFGHLVGSGKLGADLEARLRAAAATTREALEAAERVRRAFTDEVDRALERVDLLVM
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
PTLPDFPITVEEARGGRPVIAMSSLIRPFNLSGHPALSLPLPVAGSTLKAGLQIVGRHDA
CCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHCCCCCC
DEQVCAFGSHLERALAA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA