Click here to switch to the map view.

The map label for this gene is ytxM [H]

Identifier: 238023572

GI number: 238023572

Start: 75398

End: 76306

Strand: Direct

Name: ytxM [H]

Synonym: bglu_2g00740

Alternate gene names: 238023572

Gene position: 75398-76306 (Clockwise)

Preceding gene: 238023571

Following gene: 238023573

Centisome position: 2.67

GC content: 69.97

Gene sequence:

>909_bases
ATGCCGGCCGGGGAGCAGATGATGAACACGGCATCCGCCAGCAATGGATGGGTCGACAACGATGGACTGCGGCTGCACTA
TGTGATCTGGGGGCGCGACGATGCTCCCACCGTGGTGATGCTGCACGGCTTGCGCAGCTACGCACAGACCTGGGCGCCCG
TCGCCGATACGCTGATCGACCGCTACCGGGTCGTCGCTCTCGACCAGCGCGGGCGCGGGCTGAGCGACTGGGATCCCGGG
CGGGATTACCACGCGGCGGCCTATGTGCGCGATCTCGAGGCGCTGGTGCAGGCGCTCGACCTGCGACGCTTCGTGCTGGT
GGGGCACTCGATGGGCGGCGCCAACGCGTTCGTCTACGCGTCGGCTCGGCCGGAACGAATCGCCGGTCTGGCGATTGAGG
ACATGGGGCCCGGGGCGTCGGCCGGCTCGCCGGGTTCCGCACGCATCAAGCGCGAACTGCAGGACACGCCGAACGCATTC
GCCTCGTGGGACGAGGCGCGCGCGTTCTGGCGGCGCCAGCGGCCCCGCATCGCGCAAGCCGCGCTCGATTCGCGCCTCGT
GCACTCGCTCAAGGCAGATGCGCGCGGCCGGATCGTATGGCGGCACGACGCGGAAGGGATCGCGGCGGCGCGGCTGGCCG
CGACGCCCGAGCAGCTCGTCGATCTCTGGCCGCTGATCCGCGACCTGCGGGTGCCCACCTTGTTGTTGCGCGGCGGCGAT
TCGGATTTCCTGTCCGCGCAGGTGGCGGCCGAAATGGCGGCCGCCAATGCCTGCATCGAACGGATCGACATTGCCGGCGC
GACGCATTACGTGCACGACGATCAGCCGGCGGCATTCAATCGCGCGCTGCGCGGCTGGCTCGATCGTCTCGATGACGCGG
CATGGCGCGCGGGGGGGCGGGTCCAATGA

Upstream 100 bases:

>100_bases
GGTGATCTTCGCGTTCGCCACGCGCGGCCGGCTGCATGCGCGGCTCGGGGGCCTGAAGGCGAGCGAGATCGCCGGCCGCG
ACGGCCTCGTCTGATCGCGC

Downstream 100 bases:

>100_bases
AAGCGAACCACCGGCACCGCACCTCGGTCGCCATCGTCGGTGCGGGGCCGAACGGCGCGGCCATGGCGAACCTGCTCGGC
CTGTACGGCGTCGATACGAT

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MPAGEQMMNTASASNGWVDNDGLRLHYVIWGRDDAPTVVMLHGLRSYAQTWAPVADTLIDRYRVVALDQRGRGLSDWDPG
RDYHAAAYVRDLEALVQALDLRRFVLVGHSMGGANAFVYASARPERIAGLAIEDMGPGASAGSPGSARIKRELQDTPNAF
ASWDEARAFWRRQRPRIAQAALDSRLVHSLKADARGRIVWRHDAEGIAAARLAATPEQLVDLWPLIRDLRVPTLLLRGGD
SDFLSAQVAAEMAAANACIERIDIAGATHYVHDDQPAAFNRALRGWLDRLDDAAWRAGGRVQ

Sequences:

>Translated_302_residues
MPAGEQMMNTASASNGWVDNDGLRLHYVIWGRDDAPTVVMLHGLRSYAQTWAPVADTLIDRYRVVALDQRGRGLSDWDPG
RDYHAAAYVRDLEALVQALDLRRFVLVGHSMGGANAFVYASARPERIAGLAIEDMGPGASAGSPGSARIKRELQDTPNAF
ASWDEARAFWRRQRPRIAQAALDSRLVHSLKADARGRIVWRHDAEGIAAARLAATPEQLVDLWPLIRDLRVPTLLLRGGD
SDFLSAQVAAEMAAANACIERIDIAGATHYVHDDQPAAFNRALRGWLDRLDDAAWRAGGRVQ
>Mature_301_residues
PAGEQMMNTASASNGWVDNDGLRLHYVIWGRDDAPTVVMLHGLRSYAQTWAPVADTLIDRYRVVALDQRGRGLSDWDPGR
DYHAAAYVRDLEALVQALDLRRFVLVGHSMGGANAFVYASARPERIAGLAIEDMGPGASAGSPGSARIKRELQDTPNAFA
SWDEARAFWRRQRPRIAQAALDSRLVHSLKADARGRIVWRHDAEGIAAARLAATPEQLVDLWPLIRDLRVPTLLLRGGDS
DFLSAQVAAEMAAANACIERIDIAGATHYVHDDQPAAFNRALRGWLDRLDDAAWRAGGRVQ

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=267, Percent_Identity=29.2134831460674, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI23200008, Length=267, Percent_Identity=29.2134831460674, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI218777837, Length=291, Percent_Identity=24.0549828178694, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786902, Length=253, Percent_Identity=28.0632411067194, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554012, Length=302, Percent_Identity=23.841059602649, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24646055, Length=279, Percent_Identity=26.1648745519713, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33192; Mature: 33061

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGEQMMNTASASNGWVDNDGLRLHYVIWGRDDAPTVVMLHGLRSYAQTWAPVADTLID
CCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYRVVALDQRGRGLSDWDPGRDYHAAAYVRDLEALVQALDLRRFVLVGHSMGGANAFVYA
HHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEE
SARPERIAGLAIEDMGPGASAGSPGSARIKRELQDTPNAFASWDEARAFWRRQRPRIAQA
CCCCCHHHCEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCHHHHH
ALDSRLVHSLKADARGRIVWRHDAEGIAAARLAATPEQLVDLWPLIRDLRVPTLLLRGGD
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCC
SDFLSAQVAAEMAAANACIERIDIAGATHYVHDDQPAAFNRALRGWLDRLDDAAWRAGGR
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
VQ
CC
>Mature Secondary Structure 
PAGEQMMNTASASNGWVDNDGLRLHYVIWGRDDAPTVVMLHGLRSYAQTWAPVADTLID
CCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYRVVALDQRGRGLSDWDPGRDYHAAAYVRDLEALVQALDLRRFVLVGHSMGGANAFVYA
HHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEE
SARPERIAGLAIEDMGPGASAGSPGSARIKRELQDTPNAFASWDEARAFWRRQRPRIAQA
CCCCCHHHCEEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCHHHHH
ALDSRLVHSLKADARGRIVWRHDAEGIAAARLAATPEQLVDLWPLIRDLRVPTLLLRGGD
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCC
SDFLSAQVAAEMAAANACIERIDIAGATHYVHDDQPAAFNRALRGWLDRLDDAAWRAGGR
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCC
VQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8566759; 9387221; 9384377 [H]