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The map label for this gene is ydaP [H]

Identifier: 238023530

GI number: 238023530

Start: 32060

End: 33868

Strand: Reverse

Name: ydaP [H]

Synonym: bglu_2g00310

Alternate gene names: 238023530

Gene position: 33868-32060 (Counterclockwise)

Preceding gene: 238023534

Following gene: 238023529

Centisome position: 1.2

GC content: 70.76

Gene sequence:

>1809_bases
ATGACGACAGTTTCGGATTTCATCGTCGAACGACTCGCCGAATGGGGCGTCGACCGCATCTACGGCTACCCCGGCGACGG
CATCAACGGCATGTTCGGCGCGCTCAACCGCGCGCACGGAAAGATCACCTTCATCCAGGCACGCCACGAGGAAATGGCGG
CGTTCATGGCCAGCGCGCATGCCAAGTTCACCGGCAAGCTCGGCGTGTGCATCGCCACCTCCGGCCCCGGCGCCTCGCAC
CTGATCACCGGCCTCTACGACGCGCGGCTCGACCACATGCCGGTGCTCGCGATCGTCGGCCAGCAGGCACGCGCCGCGCT
CGGCGGCCACTACCAGCAGGAGCTGGACCTGCCGGCGCTGTTCAAGGACGTGGCCGGCGCCTTCGTGCAGCAGGCGAGCG
TGCCGGGGCAGGTCCGCCATCTGGTCGATCGGGCCGTGCGCACCGCGCTCGGCGCGCGCACCGTCACCGCGCTGATCCTG
CCCAACGACCTGCAGGAGCTCGACTACGCGCCGCCCGCGCGCGCCCACGGCACCGTGCATTCGGGCGTCGGCTACACGCG
CCCGAAGGTGGTGCCGTATCCGGAGGATCTGAAGCGCGCGGCCGAGGTGCTCAATGCCGGCTCGAAGGTCGCGATGCTGG
TGGGCGCCGGCGCGCTGGGCGCCACCGACCAGGTGATCGCGGTGGCCGACCGGCTCGGCGCGGGCGCGGCCAAGGCGCTG
CTCGGCAAGGCCGCGCTGCCCGACGATCTGCCCTGGGTGACCGGCGCGATCGGCCTGCTCGGCACCCGGCCGAGCTACGA
GCTGATGAACGAGTGTGACACGCTGCTGGTGGTGGGCTCCGGCTTCCCCTATGCGGAGTTCCTACCGAAGGAGGGCCAGG
CACGCGCGGTGCAGATCGACCTGAAGGCCGACATGCTGAGCCTGCGCTATCCGATGGAGGTCAACCTGGTCGGCGACAGC
GCCGAGACGCTCACCCAGCTGCTGCCGCTGCTGGCCGATCGCACCGAGCACCGCTGGCGCGACCGGATCGCGCGCTGGAA
CGAGGCATGGCGTGACACGCTGGCCAGCCGCGCGGCCGCCGACGCGAGCCCCGGGCGCGGCCTCAACCCGCAGCGCGCGT
TCACCGAACTCTCGCCGCGCCTGCCCGACGACGTGATCCTCACGAGCGACTCGGGCTCCTGCGCGAACTGGTATGCGCGC
GACCTGCAGATGCGGCGCGGCATGATGGCATCGCTGTCGGGCGGGCTCGCCTCGATGGGCGCCGCGGTGCCCTACGCGAT
CGCGGCGAAGTTCGCGTATCCGGGGCGGCCGGTGATCGCGATGGTCGGCGACGGCGCGATGCAGATGAACAACATGGCCG
AGCTGATCACCGTCGCCAAATACTGGCGGCAGTGGCAGGACCCGCGCTGGATCTGCCTCGTGCTCAACAACGAGGACCTG
AACCAGGTCACCTGGGAGCAGCGCGTGATGGAGGGCGACCCGAAGTTCGACGCCTCGCAGCAGGTGCCCGACGTGCCCTA
TCACCGCTTCGCCGAGCTGATCGGGCTCAAGGGCCTCTACGTCGATGATCCGGACCGGATCGGCGCGGCCTGGGACGACG
CGCTCGCGGCCGGCAAGCCGGTGGTGCTCGAGGTCAAGACCGATCCCGAGGTGCCGCCCCTGCCGCCGCACGTCACGCTG
CAGCAGGCCAGACAGTTCGCCGAGACGCTCATCAAGGGCGACCCGCGCGAGCGCGGCGTGATCGTCGAGACCGCGCGACA
GGTGCTGTCGGCCGTGCTGCCCGCGGGCGACCGGCACGGCAAGTCCTGA

Upstream 100 bases:

>100_bases
CCCCCCGGCGCGCGAACCGGCGGCCCGCGCGGCCTCCAGGGCGCACCGCGTAACACGGGCGGTCGCGCACGGCGGCCCAC
CGACATCAAGGAGCAACGGC

Downstream 100 bases:

>100_bases
AGCCGCGGCGGCGCGGCACGCCCGGCCGGGCGCGAGCCGGCCGAGGGCCCGCGTCCGCCGCGTCCGCCTCGTCCGTCCTG
CCCGCCTGCCCTGTTGCCAT

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MTTVSDFIVERLAEWGVDRIYGYPGDGINGMFGALNRAHGKITFIQARHEEMAAFMASAHAKFTGKLGVCIATSGPGASH
LITGLYDARLDHMPVLAIVGQQARAALGGHYQQELDLPALFKDVAGAFVQQASVPGQVRHLVDRAVRTALGARTVTALIL
PNDLQELDYAPPARAHGTVHSGVGYTRPKVVPYPEDLKRAAEVLNAGSKVAMLVGAGALGATDQVIAVADRLGAGAAKAL
LGKAALPDDLPWVTGAIGLLGTRPSYELMNECDTLLVVGSGFPYAEFLPKEGQARAVQIDLKADMLSLRYPMEVNLVGDS
AETLTQLLPLLADRTEHRWRDRIARWNEAWRDTLASRAAADASPGRGLNPQRAFTELSPRLPDDVILTSDSGSCANWYAR
DLQMRRGMMASLSGGLASMGAAVPYAIAAKFAYPGRPVIAMVGDGAMQMNNMAELITVAKYWRQWQDPRWICLVLNNEDL
NQVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGLYVDDPDRIGAAWDDALAAGKPVVLEVKTDPEVPPLPPHVTL
QQARQFAETLIKGDPRERGVIVETARQVLSAVLPAGDRHGKS

Sequences:

>Translated_602_residues
MTTVSDFIVERLAEWGVDRIYGYPGDGINGMFGALNRAHGKITFIQARHEEMAAFMASAHAKFTGKLGVCIATSGPGASH
LITGLYDARLDHMPVLAIVGQQARAALGGHYQQELDLPALFKDVAGAFVQQASVPGQVRHLVDRAVRTALGARTVTALIL
PNDLQELDYAPPARAHGTVHSGVGYTRPKVVPYPEDLKRAAEVLNAGSKVAMLVGAGALGATDQVIAVADRLGAGAAKAL
LGKAALPDDLPWVTGAIGLLGTRPSYELMNECDTLLVVGSGFPYAEFLPKEGQARAVQIDLKADMLSLRYPMEVNLVGDS
AETLTQLLPLLADRTEHRWRDRIARWNEAWRDTLASRAAADASPGRGLNPQRAFTELSPRLPDDVILTSDSGSCANWYAR
DLQMRRGMMASLSGGLASMGAAVPYAIAAKFAYPGRPVIAMVGDGAMQMNNMAELITVAKYWRQWQDPRWICLVLNNEDL
NQVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGLYVDDPDRIGAAWDDALAAGKPVVLEVKTDPEVPPLPPHVTL
QQARQFAETLIKGDPRERGVIVETARQVLSAVLPAGDRHGKS
>Mature_601_residues
TTVSDFIVERLAEWGVDRIYGYPGDGINGMFGALNRAHGKITFIQARHEEMAAFMASAHAKFTGKLGVCIATSGPGASHL
ITGLYDARLDHMPVLAIVGQQARAALGGHYQQELDLPALFKDVAGAFVQQASVPGQVRHLVDRAVRTALGARTVTALILP
NDLQELDYAPPARAHGTVHSGVGYTRPKVVPYPEDLKRAAEVLNAGSKVAMLVGAGALGATDQVIAVADRLGAGAAKALL
GKAALPDDLPWVTGAIGLLGTRPSYELMNECDTLLVVGSGFPYAEFLPKEGQARAVQIDLKADMLSLRYPMEVNLVGDSA
ETLTQLLPLLADRTEHRWRDRIARWNEAWRDTLASRAAADASPGRGLNPQRAFTELSPRLPDDVILTSDSGSCANWYARD
LQMRRGMMASLSGGLASMGAAVPYAIAAKFAYPGRPVIAMVGDGAMQMNNMAELITVAKYWRQWQDPRWICLVLNNEDLN
QVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGLYVDDPDRIGAAWDDALAAGKPVVLEVKTDPEVPPLPPHVTLQ
QARQFAETLIKGDPRERGVIVETARQVLSAVLPAGDRHGKS

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=571, Percent_Identity=23.292469352014, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1787096, Length=590, Percent_Identity=32.5423728813559, Blast_Score=268, Evalue=1e-72,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=28.1362007168459, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1790104, Length=575, Percent_Identity=26.4347826086956, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=25.668449197861, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1788716, Length=583, Percent_Identity=22.6415094339623, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=600, Percent_Identity=25, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=600, Percent_Identity=25, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17542570, Length=587, Percent_Identity=23.8500851788756, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=26.4448336252189, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6320816, Length=473, Percent_Identity=21.5644820295983, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922626, Length=584, Percent_Identity=23.6301369863014, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 65048; Mature: 64917

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVSDFIVERLAEWGVDRIYGYPGDGINGMFGALNRAHGKITFIQARHEEMAAFMASAH
CCCHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHH
AKFTGKLGVCIATSGPGASHLITGLYDARLDHMPVLAIVGQQARAALGGHYQQELDLPAL
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHCCCHHHHCCHHHH
FKDVAGAFVQQASVPGQVRHLVDRAVRTALGARTVTALILPNDLQELDYAPPARAHGTVH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHCCCCCCCCCCCCEE
SGVGYTRPKVVPYPEDLKRAAEVLNAGSKVAMLVGAGALGATDQVIAVADRLGAGAAKAL
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHH
LGKAALPDDLPWVTGAIGLLGTRPSYELMNECDTLLVVGSGFPYAEFLPKEGQARAVQID
HHCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCHHHHCCCCCCCEEEEEE
LKADMLSLRYPMEVNLVGDSAETLTQLLPLLADRTEHRWRDRIARWNEAWRDTLASRAAA
ECHHHHHEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DASPGRGLNPQRAFTELSPRLPDDVILTSDSGSCANWYARDLQMRRGMMASLSGGLASMG
CCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
AAVPYAIAAKFAYPGRPVIAMVGDGAMQMNNMAELITVAKYWRQWQDPRWICLVLNNEDL
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
NQVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGLYVDDPDRIGAAWDDALAAGKP
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEECCHHHCCCCHHHHHHCCCC
VVLEVKTDPEVPPLPPHVTLQQARQFAETLIKGDPRERGVIVETARQVLSAVLPAGDRHG
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCC
KS
CC
>Mature Secondary Structure 
TTVSDFIVERLAEWGVDRIYGYPGDGINGMFGALNRAHGKITFIQARHEEMAAFMASAH
CCHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHH
AKFTGKLGVCIATSGPGASHLITGLYDARLDHMPVLAIVGQQARAALGGHYQQELDLPAL
HHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHCCCHHHHCCHHHH
FKDVAGAFVQQASVPGQVRHLVDRAVRTALGARTVTALILPNDLQELDYAPPARAHGTVH
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHCCCCCCCCCCCCEE
SGVGYTRPKVVPYPEDLKRAAEVLNAGSKVAMLVGAGALGATDQVIAVADRLGAGAAKAL
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHH
LGKAALPDDLPWVTGAIGLLGTRPSYELMNECDTLLVVGSGFPYAEFLPKEGQARAVQID
HHCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCHHHHCCCCCCCEEEEEE
LKADMLSLRYPMEVNLVGDSAETLTQLLPLLADRTEHRWRDRIARWNEAWRDTLASRAAA
ECHHHHHEECCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DASPGRGLNPQRAFTELSPRLPDDVILTSDSGSCANWYARDLQMRRGMMASLSGGLASMG
CCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
AAVPYAIAAKFAYPGRPVIAMVGDGAMQMNNMAELITVAKYWRQWQDPRWICLVLNNEDL
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
NQVTWEQRVMEGDPKFDASQQVPDVPYHRFAELIGLKGLYVDDPDRIGAAWDDALAAGKP
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEECCHHHCCCCHHHHHHCCCC
VVLEVKTDPEVPPLPPHVTLQQARQFAETLIKGDPRERGVIVETARQVLSAVLPAGDRHG
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCC
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.03 {sodium} 2 {ferricyanide}} 0.0012 {thiamine} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]