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The map label for this gene is alkJ [H]

Identifier: 237814453

GI number: 237814453

Start: 4071133

End: 4072803

Strand: Reverse

Name: alkJ [H]

Synonym: GBP346_A4251

Alternate gene names: 237814453

Gene position: 4072803-4071133 (Counterclockwise)

Preceding gene: 237814456

Following gene: 237814452

Centisome position: 99.37

GC content: 69.9

Gene sequence:

>1671_bases
ATGTCGGCGGCAAACCAGAACGGCTCGACGGAATTCGACTACATCGTGATCGGCGGCGGTTCGGCCGGCTGCGTCGTCAC
GCACCGGCTCGTGAGCGCCGGGCATCGCGTGCTGCTGCTCGAAGCCGGCCCGCCCGACAATTCGTTCTTCGTCCACACGC
CCGCGACGTTCGTGCGGGTGATCGGCACGAAACGCACGTGGGTTTACGAGACCGAGCCGCAAGCGCACGCGGCCGGCCGC
CGGATGTACGTGCCGCAGGGCCGCACGCTCGGCGGCGGCAGCTCGGTGAACGCGATGGTCTACATCCGCGGCACGCCGGC
CGATTACGACGGCTGGCGCGACGCCGGCTGCGACGGCTGGGGCTGGGACGACGTGCTGCCGTTCTTCCGGCGCGCCGAGC
ACAATCATCGGCTCGCCGGGCCGCTGCACGGCGTCGACGGGCCGCTGCACGTAAGCGACTCGCGCTTTCGGCATCCGCTG
AGCCACGCGTTCGTCCAGGGCGCGCAGGAGTTCGGACTGCCATACAACGACGACTTCAACGGCGCGTCACAGGCAGGCGT
CGGCTTCTATCAGACGACGACGTTCGAAGGCCGGCGCGGCAGCACCGCCGCGACGTATCTCGCCGCCGTCAAGCGCGATC
CGCTGCTGACGACCGAAACCGACGCGTTCGTCACGCGCATCGTGTTCGAGAACGGCGCGGCGGTCGGCGTGCGTTACCAG
GCGCGCGACGGCGAAGAGCGGATCGCGCGTGCGCGCGCGGAAATCGTGCTGTGCGCCGGCGCGCTCGCGAGCCCGAAGCT
CCTGATGCTGTCGGGCGTCGGCCCGGCGGAGCAACTGCTGCAGCACGGCATTCCGGTGGTGCACGATTCGCCCGAGGTCG
GGCTCAACTTCCAGGATCATCTCGAAGTGTCGCTGTACGGGCGCGCGCGCGAGCCGGTCAGTCTCGCGGGGCAGGACCGC
GGGCTCAACGCGCTGCGCCACGGCATCCAGTACACGTTGTTTCATACGGGGCTGCTCACGTCGAACGTCGTCGAAAGCGG
CGGCTTCGTCGACACGGCGAACGGCGGCCGCCCGGACGTGCAGTTCCACGTGCTGCCCGTGCTCGTCGGCGACGTCGGCC
GCGAACCGCTCGCGGGCCACGGAATCTCGATCAATCCATGCTTTCTGCGGCCGAAGTCGCGCGGCACCGTGCGCCTGCGC
AGCGCCGATCCGCACGCGCCGATCCTTTTCGACGGCAATTTCCTCAGTCATCCGGACGATTTCGCGGCGCTCATGCGCGG
CCTGTCGCTCGCGCGCGAGATCATGCGGATGCCGTCGATGTCGAAGGCGATCGCGGGCGAGATGCTGCCGACCGACGGCG
GCCGCGTCGATCTCGACGCGTACGTGCGCTCGCACGCGAAGACCGTCTATCACCCGTCGGGCACGTGCCGCATGGGCGGC
GATCCGGATTCGGTCGTCGACGCGCAACTGCGCGTGCGCGGCGTCGGCGGGCTGCGGATCTGCGACGCATCGGTGATGCC
CTCGCTCGTGTCCGGCAACACCAATGCGCCGACGATCATGATCGCCGAGCGCTGCGCCGAATTCATGCTGTCGCCGGCGC
TCGCGCCGACTGCGCGCGCCGGCGCGAGTACGCCGCAAGCCGCGCCGCAGGACGCGAGCCACGCGCGCTGA

Upstream 100 bases:

>100_bases
CGCAGTTTGAATTGACCAACAGCGCACACGGCGTGCACACCGGCCGATTTAGCCGATAGTGGCAGCCTGTCCATCCATTC
GACCAAGCGAGAAGCGACAC

Downstream 100 bases:

>100_bases
GCGCCGCGCGCTCGCCGTCGCGCCGCGCGAGCGCGCGGGCCGTCACGATCGCGCTGTCACATTCATTCGAAGGAGACGTC
AGTCATGCATACGTTCAACG

Product: alcohol dehydrogenase (acceptor)

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSAANQNGSTEFDYIVIGGGSAGCVVTHRLVSAGHRVLLLEAGPPDNSFFVHTPATFVRVIGTKRTWVYETEPQAHAAGR
RMYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGWGWDDVLPFFRRAEHNHRLAGPLHGVDGPLHVSDSRFRHPL
SHAFVQGAQEFGLPYNDDFNGASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTTETDAFVTRIVFENGAAVGVRYQ
ARDGEERIARARAEIVLCAGALASPKLLMLSGVGPAEQLLQHGIPVVHDSPEVGLNFQDHLEVSLYGRAREPVSLAGQDR
GLNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDVQFHVLPVLVGDVGREPLAGHGISINPCFLRPKSRGTVRLR
SADPHAPILFDGNFLSHPDDFAALMRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGG
DPDSVVDAQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPTARAGASTPQAAPQDASHAR

Sequences:

>Translated_556_residues
MSAANQNGSTEFDYIVIGGGSAGCVVTHRLVSAGHRVLLLEAGPPDNSFFVHTPATFVRVIGTKRTWVYETEPQAHAAGR
RMYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGWGWDDVLPFFRRAEHNHRLAGPLHGVDGPLHVSDSRFRHPL
SHAFVQGAQEFGLPYNDDFNGASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTTETDAFVTRIVFENGAAVGVRYQ
ARDGEERIARARAEIVLCAGALASPKLLMLSGVGPAEQLLQHGIPVVHDSPEVGLNFQDHLEVSLYGRAREPVSLAGQDR
GLNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDVQFHVLPVLVGDVGREPLAGHGISINPCFLRPKSRGTVRLR
SADPHAPILFDGNFLSHPDDFAALMRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGG
DPDSVVDAQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPTARAGASTPQAAPQDASHAR
>Mature_555_residues
SAANQNGSTEFDYIVIGGGSAGCVVTHRLVSAGHRVLLLEAGPPDNSFFVHTPATFVRVIGTKRTWVYETEPQAHAAGRR
MYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGWGWDDVLPFFRRAEHNHRLAGPLHGVDGPLHVSDSRFRHPLS
HAFVQGAQEFGLPYNDDFNGASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTTETDAFVTRIVFENGAAVGVRYQA
RDGEERIARARAEIVLCAGALASPKLLMLSGVGPAEQLLQHGIPVVHDSPEVGLNFQDHLEVSLYGRAREPVSLAGQDRG
LNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDVQFHVLPVLVGDVGREPLAGHGISINPCFLRPKSRGTVRLRS
ADPHAPILFDGNFLSHPDDFAALMRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGGD
PDSVVDAQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPTARAGASTPQAAPQDASHAR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=549, Percent_Identity=40.9836065573771, Blast_Score=390, Evalue=1e-108,
Organism=Escherichia coli, GI1786503, Length=554, Percent_Identity=41.1552346570397, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17532301, Length=542, Percent_Identity=34.5018450184502, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24642042, Length=564, Percent_Identity=38.2978723404255, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI45549471, Length=565, Percent_Identity=37.3451327433628, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI45551458, Length=565, Percent_Identity=37.3451327433628, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24642048, Length=581, Percent_Identity=35.1118760757315, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24642055, Length=569, Percent_Identity=37.2583479789104, Blast_Score=298, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24642059, Length=564, Percent_Identity=36.8794326241135, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24650267, Length=567, Percent_Identity=34.0388007054674, Blast_Score=277, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24642051, Length=574, Percent_Identity=34.1463414634146, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI17137792, Length=550, Percent_Identity=34, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI18859995, Length=565, Percent_Identity=33.4513274336283, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24642039, Length=566, Percent_Identity=33.7455830388693, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24642037, Length=575, Percent_Identity=34.4347826086956, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI18859993, Length=579, Percent_Identity=34.3696027633851, Blast_Score=249, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24642035, Length=305, Percent_Identity=39.0163934426229, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24645930, Length=573, Percent_Identity=27.7486910994764, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24642057, Length=580, Percent_Identity=28.7931034482759, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59466; Mature: 59335

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAANQNGSTEFDYIVIGGGSAGCVVTHRLVSAGHRVLLLEAGPPDNSFFVHTPATFVRV
CCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEECHHHHHHH
IGTKRTWVYETEPQAHAAGRRMYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGW
HCCCCEEEEECCCCHHHCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
GWDDVLPFFRRAEHNHRLAGPLHGVDGPLHVSDSRFRHPLSHAFVQGAQEFGLPYNDDFN
CHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHCHHHHHHHHHHHHHCCCCCCCCC
GASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTTETDAFVTRIVFENGAAVGVRYQ
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEECHHHHEEEEEECCCCEEEEEEE
ARDGEERIARARAEIVLCAGALASPKLLMLSGVGPAEQLLQHGIPVVHDSPEVGLNFQDH
CCCCHHHHHHHHHEEEEEECCCCCCCEEEEECCCHHHHHHHCCCCEEECCCCCCCCHHHC
LEVSLYGRAREPVSLAGQDRGLNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDV
EEEEEECCCCCCCEECCCCCCHHHHHHCCEEEEEEHHHHHHHHHHCCCEEECCCCCCCCE
QFHVLPVLVGDVGREPLAGHGISINPCFLRPKSRGTVRLRSADPHAPILFDGNFLSHPDD
EEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCEEECCCCCCCCHH
FAALMRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGG
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEHHHHHHHCCCEEECCCCCEECCC
DPDSVVDAQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPTARA
CCCHHEEEEEEEECCCCEEEECHHHCCHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCC
GASTPQAAPQDASHAR
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAANQNGSTEFDYIVIGGGSAGCVVTHRLVSAGHRVLLLEAGPPDNSFFVHTPATFVRV
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEECHHHHHHH
IGTKRTWVYETEPQAHAAGRRMYVPQGRTLGGGSSVNAMVYIRGTPADYDGWRDAGCDGW
HCCCCEEEEECCCCHHHCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
GWDDVLPFFRRAEHNHRLAGPLHGVDGPLHVSDSRFRHPLSHAFVQGAQEFGLPYNDDFN
CHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHCHHHHHHHHHHHHHCCCCCCCCC
GASQAGVGFYQTTTFEGRRGSTAATYLAAVKRDPLLTTETDAFVTRIVFENGAAVGVRYQ
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEECHHHHEEEEEECCCCEEEEEEE
ARDGEERIARARAEIVLCAGALASPKLLMLSGVGPAEQLLQHGIPVVHDSPEVGLNFQDH
CCCCHHHHHHHHHEEEEEECCCCCCCEEEEECCCHHHHHHHCCCCEEECCCCCCCCHHHC
LEVSLYGRAREPVSLAGQDRGLNALRHGIQYTLFHTGLLTSNVVESGGFVDTANGGRPDV
EEEEEECCCCCCCEECCCCCCHHHHHHCCEEEEEEHHHHHHHHHHCCCEEECCCCCCCCE
QFHVLPVLVGDVGREPLAGHGISINPCFLRPKSRGTVRLRSADPHAPILFDGNFLSHPDD
EEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCEEECCCCCCCCHH
FAALMRGLSLAREIMRMPSMSKAIAGEMLPTDGGRVDLDAYVRSHAKTVYHPSGTCRMGG
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEHHHHHHHCCCEEECCCCCEECCC
DPDSVVDAQLRVRGVGGLRICDASVMPSLVSGNTNAPTIMIAERCAEFMLSPALAPTARA
CCCHHEEEEEEEECCCCEEEECHHHCCHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCCC
GASTPQAAPQDASHAR
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]