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The map label for this gene is braF [H]

Identifier: 237814445

GI number: 237814445

Start: 4063308

End: 4064030

Strand: Reverse

Name: braF [H]

Synonym: GBP346_A4243

Alternate gene names: 237814445

Gene position: 4064030-4063308 (Counterclockwise)

Preceding gene: 237814446

Following gene: 237814444

Centisome position: 99.16

GC content: 70.54

Gene sequence:

>723_bases
ATGATCGAAATCTCGAACCTGACCGTGCAATTCGGCGGCACGCGCGTGCTCGACGCGCTCGACGCGAAGCTCGCCGCGCC
CATATGCGGGCTGATCGGCCCGAACGGCGCGGGCAAGACGACGCTGCTGAACGTACTGAGCGGCTTCTTGCGGCCGCGCG
CGGGCAGCGTCGCGCTGGACGGCCGCGCGCTGCTCGCGCTGTCCGTCGCCGAGCGCGTGCGCGCGGGCGTGCGGCGCACG
TTCCAGACCGAACAGATCGTCGAGGATCTGAGCGTGCACGACAACGTGCTCGCGCTCGCCGAGCACGTGATGCCGGCTCT
CGCCGCGCGCGACGACACGCGGCGCGCGCTCGAACTCGTCGGCCTCGCCGACGTCGCGCACGTGCAGGGCGCGGCGCTCA
ACCTGTATCAGCGCCGCATGCTGGAACTCGGCAAGGCGCTCGTCGGCGCGCCGCGCCTGCTGCTGCTCGACGAGCCGGGC
GCGGGCCTGAACGAGACCGAAGCCGCGCGGCTCGCCGACGTGATCGTTCGCATTCCCGAATGCGTCGGCGCGCAGGTGCT
GCTGATCGATCACGACGTCGACCTGATCGATGCCGTGTGCGAGGCGACGCTCGTGCTCGATTTCGGCAAGCGGCTCGCGC
TCGGGCCCACGCGCGCGGTGCTCGACGATCCGCTCGTGCGCAGTGCGTATCTCGGCCAAGCGTTCGACGAACATGAAGCG
TGA

Upstream 100 bases:

>100_bases
TGCATGCGCTCGTCACGGCGCCCGCCGGCATCGCCGGGCAGCTTGCCGGGCTCGCGTCGCGGGTCGGGCGCGCGTTTCGC
GCCCGCGACGGAGGCGCGCG

Downstream 100 bases:

>100_bases
CGCGAAGCGCCGGATCGGCCGGCCGGCGTCGAATCGCCGCTCGTGCACGAGCGCGGGCGCGAGTGCCCGTGCGAACGGCG
CCCGAACGGCGTGCGGCGCG

Product: branched-chain amino acid ABC transporter, ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALDGRALLALSVAERVRAGVRRT
FQTEQIVEDLSVHDNVLALAEHVMPALAARDDTRRALELVGLADVAHVQGAALNLYQRRMLELGKALVGAPRLLLLDEPG
AGLNETEAARLADVIVRIPECVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHEA

Sequences:

>Translated_240_residues
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALDGRALLALSVAERVRAGVRRT
FQTEQIVEDLSVHDNVLALAEHVMPALAARDDTRRALELVGLADVAHVQGAALNLYQRRMLELGKALVGAPRLLLLDEPG
AGLNETEAARLADVIVRIPECVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHEA
>Mature_240_residues
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALDGRALLALSVAERVRAGVRRT
FQTEQIVEDLSVHDNVLALAEHVMPALAARDDTRRALELVGLADVAHVQGAALNLYQRRMLELGKALVGAPRLLLLDEPG
AGLNETEAARLADVIVRIPECVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=192, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI27477115, Length=181, Percent_Identity=29.2817679558011, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI153792144, Length=170, Percent_Identity=31.7647058823529, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI27881501, Length=212, Percent_Identity=24.0566037735849, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI30795238, Length=212, Percent_Identity=24.0566037735849, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1789864, Length=252, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789593, Length=235, Percent_Identity=32.3404255319149, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786563, Length=261, Percent_Identity=30.6513409961686, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786398, Length=243, Percent_Identity=30.4526748971193, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87081791, Length=231, Percent_Identity=27.7056277056277, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788472, Length=250, Percent_Identity=26.4, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87081709, Length=243, Percent_Identity=25.9259259259259, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789991, Length=234, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788761, Length=216, Percent_Identity=28.7037037037037, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082268, Length=234, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787029, Length=228, Percent_Identity=26.7543859649123, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789873, Length=220, Percent_Identity=31.3636363636364, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789586, Length=231, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790525, Length=236, Percent_Identity=25, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI48995001, Length=243, Percent_Identity=26.3374485596708, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786872, Length=228, Percent_Identity=25.8771929824561, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787712, Length=193, Percent_Identity=26.9430051813472, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI48994997, Length=243, Percent_Identity=27.9835390946502, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788897, Length=214, Percent_Identity=28.5046728971963, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI87081782, Length=198, Percent_Identity=31.3131313131313, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787164, Length=197, Percent_Identity=35.0253807106599, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787370, Length=237, Percent_Identity=23.6286919831224, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788225, Length=234, Percent_Identity=25.6410256410256, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787792, Length=223, Percent_Identity=25.5605381165919, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790544, Length=249, Percent_Identity=28.1124497991968, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081834, Length=178, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788450, Length=229, Percent_Identity=26.2008733624454, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI2367384, Length=213, Percent_Identity=28.169014084507, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789672, Length=214, Percent_Identity=24.2990654205607, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788506, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994943, Length=226, Percent_Identity=24.3362831858407, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788210, Length=191, Percent_Identity=27.7486910994764, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790739, Length=238, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789032, Length=222, Percent_Identity=26.1261261261261, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786345, Length=220, Percent_Identity=26.8181818181818, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1790467, Length=218, Percent_Identity=25.6880733944954, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=222, Percent_Identity=25.2252252252252, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=220, Percent_Identity=28.1818181818182, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=220, Percent_Identity=28.1818181818182, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=220, Percent_Identity=28.1818181818182, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=218, Percent_Identity=30.2752293577982, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=211, Percent_Identity=25.5924170616114, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221512771, Length=212, Percent_Identity=25.4716981132075, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24643674, Length=202, Percent_Identity=26.7326732673267, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25484; Mature: 25484

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALD
CEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEC
GRALLALSVAERVRAGVRRTFQTEQIVEDLSVHDNVLALAEHVMPALAARDDTRRALELV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GLADVAHVQGAALNLYQRRMLELGKALVGAPRLLLLDEPGAGLNETEAARLADVIVRIPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
CVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHEA
HCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCHHHHCCHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MIEISNLTVQFGGTRVLDALDAKLAAPICGLIGPNGAGKTTLLNVLSGFLRPRAGSVALD
CEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEC
GRALLALSVAERVRAGVRRTFQTEQIVEDLSVHDNVLALAEHVMPALAARDDTRRALELV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GLADVAHVQGAALNLYQRRMLELGKALVGAPRLLLLDEPGAGLNETEAARLADVIVRIPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
CVGAQVLLIDHDVDLIDAVCEATLVLDFGKRLALGPTRAVLDDPLVRSAYLGQAFDEHEA
HCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCHHHHCCHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]